January 2024

Cover articles

  1. Pastoral support for students

    Jennifer Tullet, Jennifer Leigh ... Emma Hargreaves
    1. Cell Biology
    2. Neuroscience

    DKK3 and Alzheimer’s disease

    Nuria Martin Flores, Marina Podpolny ... Patricia C Salinas
    1. Physics of Living Systems

    Vocal motifs in orangutans

    Adriano R Lameira, Madeleine E Hardus ... Marco Gamba
    1. Cell Biology

    Secretion secrets

    Sara Chelaghma, Huiling Ke ... Ross F Waller

Highlights controls:

Research articles

    1. Medicine

    Tumor Purity-Related Genes for Predicting the Prognosis and Drug Sensitivity of DLBCL Patients

    Zhenbang Ye, Ning Huang ... Wenting Huang
    1. Cell Biology
    2. Neuroscience

    Cardiac glycosides restore autophagy flux in an iPSC-derived neuronal model of WDR45 deficiency

    Apostolos Papandreou, Nivedita Singh ... Robin Ketteler
    1. Computational and Systems Biology

    Detection of new pioneer transcription factors as cell-type-specific nucleosome binders

    Yunhui Peng, Wei Song ... Anna R Panchenko
    A computational method has been developed for predicting the cell-type-specific binding of transcription factors to nucleosomes, and involves integration of ChIP-seq, MNase-seq, and DNase-seq data with details of nucleosome structure.
    1. Physics of Living Systems

    The ability to sense the environment is heterogeneously distributed in cell populations

    Andrew Goetz, Hoda Akl, Purushottam Dixit
    Information transduction capacity of mammalian cells is high and varies substantially from cell to cell.
    1. Microbiology and Infectious Disease

    A dynamic bactofilin cytoskeleton cooperates with an M23 endopeptidase to control bacterial morphogenesis

    Sebastian Pöhl, Manuel Osorio-Valeriano ... Martin Thanbichler
    Analyses of the stalked budding bacterium Hyphomonas neptunium and its spiral-shaped relative Rhodospirillum rubrum reveal a conserved morphogenetic module that controls the establishment of complex bacterial cell shapes.
    1. Plant Biology

    Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet

    Carla de la Fuente, Alexandre Grondin ... Laurent Laplaze
    Increased primary root growth in pearl millet is correlated with early water stress tolerance, an important constraint in agrosystems in the Sahel, and is associated with the regulation of root cell elongation by a glutaredoxin.
    1. Cancer Biology

    STAT3 is a genetic modifier of TGF-beta induced EMT in KRAS mutant pancreatic cancer

    Stephen D’Amico, Varvara Kirillov ... Nancy C. Reich
    1. Cell Biology

    Autoacetylation-mediated phase separation of TIP60 is critical for its functions

    Shraddha Dubey, Himanshu Gupta, Ashish Gupta
    1. Cancer Biology

    Targeting the Hippo pathway in cancers via ubiquitination dependent TEAD degradation

    Trang H. Pham, Kanika Bajaj Pahuja ... Anwesha Dey
    1. Medicine

    Loss of CTRP10 results in female obesity with preserved metabolic health

    Fangluo Chen, Dylan C. Sarver ... G. William Wong
    1. Cell Biology

    CEP44 is required for maintaining centriole duplication and spindle integrity

    Donghui Zhang, Wenlu Wei ... Jianguo Chen
    1. Evolutionary Biology
    2. Genetics and Genomics

    Stable population structure in Europe since the Iron Age, despite high mobility

    Margaret L Antonio, Clemens L Weiß ... Jonathan K Pritchard
    Reconstructing human mobility patterns using historical period genomes illustrates how the Roman Empire’s military and economic activities catalyzed an era of transient movement against the backdrop of generations-long prehistoric migrations.
    1. Structural Biology and Molecular Biophysics

    Explicit ion modeling predicts physicochemical interactions for chromatin organization

    Xingcheng Lin, Bin Zhang
    The physicochemical interactions among wild-type nucleosomes hold substantial significance and play a role in chromatin folding under physiological salt concentrations.
    1. Neuroscience

    Spatial transcriptomics and single-nucleus RNA sequencing reveal a transcriptomic atlas of adult human spinal cord

    Donghang Zhang, Yali Chen ... Cheng Zhou
    The molecular, cellular, and spatial heterogeneity of adult human spinal cord serve as an important resource to explore the mechanisms underlying spinal cord physiology and diseases.
    1. Genetics and Genomics
    2. Neuroscience

    Orai-mediated calcium entry determines activity of central dopaminergic neurons by regulation of gene expression

    Rishav Mitra, Shlesha Richhariya, Gaiti Hasan
    Expression of the appropriate repertoire of membrane channels, essential for activity of flight-promoting central dopaminergic neurons of Drosophila, requires store-operated Ca2+ entry during late pupal development and early adults.
    1. Genetics and Genomics

    Targeting sex determination to suppress mosquito populations

    Ming Li, Nikolay P Kandul ... Omar S Akbari
    Genetic analyses and mathematical modeling reveal that disrupting genes essential for sex determination and fertility in Aedes aegypti leads to the production of sterile males, effectively suppressing mosquito populations.
    1. Neuroscience

    Behavioral entrainment to rhythmic auditory stimulation can be modulated by tACS depending on the electrical stimulation field properties

    Yuranny Cabral-Calderin, Daniela van Hinsberg ... Molly J Henry
    Transcranial alternating current stimulation modulates behavioral entrainment to sounds.
    1. Cell Biology
    2. Developmental Biology

    Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function

    Chao Chang, Sara L Banerjee ... Artur Kania
    Extracellular signals mediated by the EphB2 receptor tyrosine kinase may access the effectors of fundamental cell functions controlled by MYCBP2.
    1. Neuroscience

    Coupling of Slack and NaV1.6 sensitizes Slack to quinidine blockade and guides anti-seizure strategy development

    Tian Yuan, Yifan Wang ... Zhuo Huang
    Unveiling NaV1.6's role in sensitizing Slack to quinidine disruption redefines KCNT1-related epilepsy treatment strategies.
    1. Neuroscience

    SRF-deficient astrocytes provide neuroprotection in mouse models of excitotoxicity and neurodegeneration

    Surya Chandra Rao Thumu, Monika Jain ... Narendrakumar Ramanan
    Serum response factor (SRF) deficient reactive astrocytes are neuroprotective in the mammalian brain.
    1. Structural Biology and Molecular Biophysics

    Discovery of lipid binding sites in a ligand-gated ion channel by integrating simulations and cryo-EM

    Cathrine Bergh, Urška Rovšnik ... Erik Lindahl
    A combination of molecular dynamics simulations and electron cryomicroscopy characterizes interactions of a pentameric ligand-gated ion channel with 25 lipid molecules, specifically in a functional state in which they were not previously observed.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Heat stress-induced activation of MAPK pathway attenuates Atf1-dependent epigenetic inheritance of heterochromatin in fission yeast

    Li Sun, Libo Liu ... Quan-wen Jin
    Fission yeast loses its heterochromatin stability at mating-type locus under high temperature due to compromised binding between heterochromatin protein Swi6HP1 and Atf1.
    1. Developmental Biology

    Gene regulatory patterning codes in early cell fate specification of the C. elegans embryo

    Alison G Cole, Tamar Hashimshony ... Itai Yanai
    The transcriptomes of individual cells of the 1- to 102-cell stages in Caenorhabditis elegans embryogenesis are identified along with 119 embryonic cell states during cell fate specification, including ‘equivalence-group’ cell identities.
    1. Cell Biology
    2. Neuroscience

    Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model

    Nuria Martin Flores, Marina Podpolny ... Patricia C Salinas
    The Wnt antagonist DKK3 is a key regulator of excitatory and inhibitory synapses, and its downregulation in the hippocampus restores synaptic connectivity and memory in an Alzheimer's disease mouse model.
    1. Neuroscience

    osl-dynamics, a toolbox for modeling fast dynamic brain activity

    Chetan Gohil, Rukuang Huang ... Mark W Woolrich
    A generative-model-based, unsupervised learning toolbox for characterizing oscillatory bursting and brain network dynamics in univariate or multivariate time series.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Ebola virus sequesters IRF3 in viral inclusion bodies to evade host antiviral immunity

    Lin Zhu, Jing Jin ... Cheng Cao
    Ebola virus VP35 protein evades host antiviral immunity by interacting with STING to sequester IRF3 into inclusion bodies and inhibit type I interferon production.
    1. Plant Biology

    The tRNA thiolation-mediated translational control is essential for plant immunity

    Xueao Zheng, Hanchen Chen ... Shunping Yan
    The transfer RNA thiolation modification is required for the efficient translation of the master immune regulator NPR1 in plants.
    1. Epidemiology and Global Health

    Region of Attainable Redaction, an extension of Ellipse of Insignificance analysis for gauging impacts of data redaction in dichotomous outcome trials

    David Robert Grimes
    Region of Attainable Redaction analysis allows scientists to estimate the potential impacts of redaction in biomedical science, helping to uncover potentially spurious or non-robust results.
    1. Neuroscience

    Feedback inhibition by a descending GABAergic neuron regulates timing of escape behavior in Drosophila larvae

    Jiayi Zhu, Jean-Christophe Boivin ... Tomoko Ohyama
    1. Neuroscience

    Perceptual error based on Bayesian cue combination drives implicit motor adaptation

    Zhaoran Zhang, Huijun Wang ... Kunlin Wei
    1. Genetics and Genomics

    Association of genetic variation in COL11A1 with adolescent idiopathic scoliosis

    Hao Yu, Anas M Khanshour ... Carol A Wise
    Variation in type XI collagen alters estrogen-dependent signaling in the extracellular matrix and is identified as a genetic risk factor for adolescent idiopathic scoliosis, a common childhood deformity.
    1. Cell Biology

    Calcium transients trigger switch-like discharge of prostaglandin E2 in an extracellular signal-regulated kinase-dependent manner

    Tetsuya Watabe, Shinya Yamahira ... Kenta Terai
    Prostaglandin E2-mediated radial spread of PKA activation, caused by calcium transients, is a novel mode of intercellular communication in living mice.
    1. Immunology and Inflammation

    Variation in the basal immune state and implications for disease

    Aisha Souquette, Paul G Thomas
    An integrative conceptual and mathematical framework of immune variation that includes unique basal profiles associated with differential susceptibility to disease, distinct acute immune signatures associated with disease severity, and how different determinants can lead to individual pathways of illness outcome.
    1. Chromosomes and Gene Expression

    Intrinsic protein disorder is insufficient to drive subnuclear clustering in embryonic transcription factors

    Colleen E Hannon, Michael B Eisen
    A broad, live imaging-based survey of intrinsic protein disorder in Drosophila transcription factors reveals a limited role for their contribution to the protein clustering observed at enhancers.
    1. Chromosomes and Gene Expression
    2. Evolutionary Biology

    Tempo and mode of gene expression evolution in the brain across primates

    Katherine Rickelton, Trisha M Zintel ... Courtney C Babbitt
    Larger phylogenetic context is required to understand human and nonhuman primate brain evolution.
    1. Developmental Biology

    Coordination of cell cycle and morphogenesis during organ formation

    Jeffrey Matthew, Vishakha Vishwakarma ... SeYeon Chung
    The SP1/KLF transcription factor Huckebein controls endoreplication in the Drosophila salivary gland, ensuring proper morphogenesis during organ formation.
    1. Cell Biology

    Exceptional longevity of mammalian ovarian and oocyte macromolecules throughout the reproductive lifespan

    Ewa K. Bomba-Warczak, Karen M. Velez ... Francesca E. Duncan
    1. Biochemistry and Chemical Biology
    2. Evolutionary Biology

    Fitness landscape of substrate-adaptive mutations in evolved APC transporters

    Foteini Karapanagioti, Úlfur Á. Atlason ... Sebastian Obermaier
    1. Microbiology and Infectious Disease

    A novel computational pipeline for var gene expression augments the discovery of changes in the Plasmodium falciparum transcriptome during transition from in vivo to short-term in vitro culture

    Clare Andradi-Brown, Jan Stephan Wichers-Misterek ... Anna Bachmann
    An enhanced bioinformatic pipeline to quantify Plasmodium falciparum core gene and polymorphic var gene expression revealed changes occurring during early culture adaptation of parasites from naturally infected individuals.
    1. Immunology and Inflammation

    Kidins220 regulates the development of B cells bearing the λ light chain

    Anna-Maria Schaffer, Gina Jasmin Fiala ... Susana Minguet
    The scaffold protein Kidins220 regulates the development of λLC B cells by supporting B cell precursor survival and optimizing pre‑BCR and BCR signaling.
    1. Cell Biology
    2. Developmental Biology

    Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice

    Longjie Sun, Zheng Lv ... Jiali Liu
    SRSF1 plays a critical role in posttranscriptional regulation to implement homing of precursor spermatogonial stem cells.
    1. Neuroscience

    New genetic tools for mushroom body output neurons in Drosophila

    Gerald M Rubin, Yoshinori Aso
    Improved cell-type-specific genetic drivers for the study of learning and memory and other complex behaviors in Drosophila are provided and validated.
    1. Computational and Systems Biology
    2. Microbiology and Infectious Disease

    Light-inducible protein degradation in E. coli with the LOVdeg tag

    Nathan Tague, Cristian Coriano-Ortiz ... Mary J Dunlop
    The LOVdeg tag is a versatile tool for bacterial optogenetics, offering modular, blue light-inducible protein degradation for a range of synthetic biology and metabolic engineering applications.
    1. Computational and Systems Biology
    2. Genetics and Genomics

    Comparative interactome analysis of α-arrestin families in human and Drosophila

    Kyung-Tae Lee, Inez KA Pranoto ... Jin-Wu Nam
    Unveiling the high-confidence protein–protein interaction networks of human and fly α-arrestins provides insights into conserved and species-specific functional roles depending on their protein domains and motifs.
    1. Neuroscience

    Involvement of superior colliculus in complex figure detection of mice

    J Leonie Cazemier, Robin Haak ... J Alexander Heimel
    Mouse superior colliculus is involved in figure detection based on differences in contrast and texture orientation and phase.
    1. Neuroscience

    A system of feed-forward cerebellar circuits that extend and diversify sensory signaling

    Harsh N Hariani, A Brynn Algstam ... Timothy S Balmer
    Unipolar brush cells subtypes, classified by their excitatory or inhibitory response to glutamate, form circuits with one another that may enhance the capacity of the cerebellum to transform input signals.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Exploring the K+ binding site and its coupling to transport in the neurotransmitter:sodium symporter LeuT

    Solveig G Schmidt, Andreas Nygaard ... Claus J Loland
    Potassium presumably binds to the Na1 site in LeuT, playing a role in inwardly rectifying the transport of substrates.
    1. Cell Biology

    Apical annuli are specialised sites of post-invasion secretion of dense granules in Toxoplasma

    Sara Chelaghma, Huiling Ke ... Ross F Waller
    Apicomplexan parasites secrete proteins to manipulate their hosts from sub-apical openings in their cell pellicle that are distinct from the apical complex from where invasion factors are secreted.
    1. Immunology and Inflammation

    Self-inhibiting percolation and viral spreading in epithelial tissue

    Xiaochan Xu, Bjarke Frost Nielsen, Kim Sneppen
    1. Neuroscience

    The involvement of the human prefrontal cortex in the emergence of visual awareness

    Zepeng Fang, Yuanyuan Dang ... Mingsha Zhang
    While minimizing the report-related motor confounding, event-related potential (ERP), high-frequency power, and functional connectivity of the local field potential (LFP) activity in human prefrontal cortex were enhanced under the emergence of visual awareness.
    1. Cell Biology
    2. Developmental Biology

    Dally is not essential for Dpp spreading or internalization but for Dpp stability by antagonizing Tkv-mediated Dpp internalization

    Niklas Simon, Abu Safyan ... Shinya Matsuda
    The heparan sulfate chains of the glypican Dally stabilizes morphogen Dpp on the cell surface by antagonizing receptor-mediated Dpp internalization largely independent of direct interaction with Dpp.
    1. Evolutionary Biology
    2. Medicine

    Taste shaped the use of botanical drugs

    Marco Leonti, Joanna Baker ... Julie Hawkins
    Plant drugs used by ancient Graeco-Roman societies have tastes and flavours that predict how they were used therapeutically.
    1. Genetics and Genomics

    Yeast eIF2A has a minimal role in translation initiation and uORF-mediated translational control in vivo

    Swati Gaikwad, Fardin Ghobakhlou ... Alan G Hinnebusch
    Eliminating eukaryotic initiation factor 2 (eIF2) from wild-type budding yeast had little impact on translation of individual mRNAs, including those with regulatory upstream open-reading frames, even when canonical initiation factor eIF2 activity was impaired.
    1. Neuroscience

    Passive exposure to task-relevant stimuli enhances categorization learning

    Christian Schmid, Muhammad Haziq ... Santiago Jaramillo
    Mice learn a categorization task faster when exposed to task-relevant stimuli outside training, supporting neural-network models in which unsupervised learning first captures the statistical structure of inputs before classifying them.
    1. Cell Biology

    Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

    Yin Luo, Huanmeng Hao ... Ding Xu
    By binding to TRAIL and inducing its oligomerization, heparan sulfate plays a role in regulating the pro-apoptotic activity of TRAIL towards several types of tumor cells.
    1. Evolutionary Biology

    Measures of genetic diversification in somatic tissues at bulk and single-cell resolution

    Marius E Moeller, Nathaniel V Mon Père ... Weini Huang
    Single-cell and bulk sequencing data are combined through theoretical modeling to reveal the number of tissue-specific stem cells, mutation, and proliferation rates under sampling.
    1. Microbiology and Infectious Disease

    Physiological and metabolic insights into the first cultured anaerobic representative of deep-sea Planctomycetes bacteria

    Rikuan Zheng, Chong Wang ... Chaomin Sun
    A deep-sea Planctomycetes bacterium performs a unique budding mode of division and recruits chronic phages for metabolizing nitrogen through the function of auxiliary metabolic genes.
    1. Genetics and Genomics
    2. Neuroscience

    The gene expression landscape of the human locus coeruleus revealed by single-nucleus and spatially-resolved transcriptomics

    Lukas M Weber, Heena R Divecha ... Stephanie C Hicks
    Spatially-resolved transcriptomics and single-nucleus RNA-sequencing are applied to the human locus coeruleus to characterize the gene expression profiles of norepinephrine (NE) neurons and other cell populations in this critical brain region, with all data made publicly available.
    1. Developmental Biology

    Sperm induction of somatic cell-cell fusion as a novel functional test

    Nicolas G Brukman, Clari Valansi, Benjamin Podbilewicz
    The fertilizing potential of sperm correlates with their ability to induce viral-like fusion of somatic cells expressing JUNO.
    1. Developmental Biology

    SMAD4 promotes somatic-germline contact during oocyte growth

    Sofia Granados-Aparici, Qin Yang, Hugh Clarke
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Tracing the substrate translocation mechanism in P-glycoprotein

    Theresa Gewering, Deepali Waghray ... Qinghai Zhang
    New P-glycoprotein structures reveal a substrate translocation mechanism and pathway across lipid bilayers that challenges ATP-binding cassette transporter norms.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Cis-regulatory modes of Ultrabithorax inactivation in butterfly forewings

    Amruta Tendolkar, Anyi Mazo-Vargas ... Arnaud Martin
    CRISPR knock-outs and functional genomic approaches probe the regulatory mechanism restricting the Hox gene Ubx to butterfly hindwings, explaining color pattern differentiation from forewings.
    1. Neuroscience

    Designing optimal behavioral experiments using machine learning

    Simon Valentin, Steven Kleinegesse ... Christopher G Lucas
    Recent advances in Bayesian optimal experimental design have made it possible to improve the efficiency and informativeness of experiments using machine learning, and shed new light on considerations that affect machine learning assisted experimental designs and computational models in general.
    1. Medicine

    Dengue Viremia Kinetics and Effects on Platelet Count and Clinical Outcomes: An Analysis of 2340 Patients from Vietnam

    Nguyen Lam Vuong, Nguyen Than Ha Quyen ... Ronald B. Geskus
    1. Microbiology and Infectious Disease

    The effect of combining antibiotics on resistance: A systematic review and meta-analysis

    Berit Siedentop, Viacheslav N. Kachalov ... Sebastian Bonhoeffer
    1. Cell Biology
    2. Computational and Systems Biology

    Reprogramming of cardiac phosphoproteome, proteome, and transcriptome confers resilience to chronic adenylyl cyclase-driven stress

    Jia-Hua Qu, Khalid Chakir ... Edward G Lakatta
    Numerous pathways/function predictions were identified via gene sets, phosphopeptides, and phosphoproteins, which may point to potential novel therapeutic targets to enhance heart adaptivity, maintaining heart performance while avoiding cardiac dysfunction.
    1. Biochemistry and Chemical Biology

    Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space

    Fabio Lolicato, Julia P Steringer ... Walter Nickel
    Disulfide-bridged fibroblast growth factor 2 (FGF2) dimerization at the inner plasma membrane leaflet produces the building block for higher FGF2 oligomers that drive FGF2 membrane translocation into the extracellular space.
    1. Biochemistry and Chemical Biology
    2. Immunology and Inflammation

    Design principles for inflammasome inhibition by pyrin-only-proteins

    Shuai Wu, Archit Garg ... Jungsan Sohn
    Computational analyses and biochemical measurements redefine the mechanisms by which pyrin-only-proteins specifically target and regulate the assembly of inflammasomes.
    1. Chromosomes and Gene Expression
    2. Immunology and Inflammation

    Regulation of nuclear transcription by mitochondrial RNA in endothelial cells

    Kiran Sriram, Zhijie Qi ... Zhen Bouman Chen
    Mitochondrial RNAs attach to chromatin and regulate nuclear transcription in endothelial stress response.
    1. Ecology
    2. Physics of Living Systems

    Investigating macroecological patterns in coarse-grained microbial communities using the stochastic logistic model of growth

    William R Shoemaker, Jacopo Grilli
    Microbial macroecological patterns under coarse-graining are captured by a null model of ecology, a result which was leveraged to successfully predict the relationship between fine and coarse-grained diversity.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions

    Jixing Lyu, Tianqi Zhang ... Arthur Laganowsky
    Native mass spectrometry reveals the thermodynamic basis for high-affinity lipid binding to the ABC transporter MsbA.
    1. Microbiology and Infectious Disease

    Coordinated regulation of gene expression in Plasmodium female gametocytes by two transcription factors

    Yuho Murata, Tsubasa Nishi ... Masao Yuda
    Sequential expression of two transcription factors that bind to five- and ten-base female-specific cis-acting elements, respectively, promotes differentiation of Plasmodium female gametocytes.
    1. Cell Biology

    Phosphate starvation signaling increases mitochondrial membrane potential through respiration-independent mechanisms

    Yeyun Ouyang, Mi-Young Jeong ... Jared Rutter
    SIT4 deletion and phosphate starvation increase mitochondrial membrane potential via electron transport chain dependent and independent manners, and the increased membrane potential induced by phosphate depletion is conserved between yeast, flies, and humans.
    1. Physics of Living Systems

    Recursive self-embedded vocal motifs in wild orangutans

    Adriano R Lameira, Madeleine E Hardus ... Marco Gamba
    Wild male orangutans produce long calls composed of two hierarchical layers, wherein isochronous sub-pulses occur within isochronous pulses, indicating that recursive vocal combinatorics are not exclusive to language among hominids.
    1. Cancer Biology

    Ferredoxin 1 is essential for embryonic development and lipid homeostasis

    Shakur Mohibi, Yanhong Zhang ... Xinbin Chen
    The iron-sulfur cluster containing Ferredoxin 1 (FDX1), critical in steroidogenesis and TCA cycle, is required for mammalian embryonic development and maintenance of lipid homeostasis at cellular and organismal levels.
    1. Microbiology and Infectious Disease

    Interplay between acetylation and ubiquitination of imitation switch chromatin remodeler Isw1 confers multidrug resistance in Cryptococcus neoformans

    Yang Meng, Yue Ni ... Chen Ding
    Isw1 acts as a master regulator in modulating the expression of drug-resistance genes, and this regulatory mechanism is dependent on the interaction of Isw1 acetylation and ubiquitination.
    1. Neuroscience
    2. Computational and Systems Biology

    Inter-regional delays fluctuate in the human cerebral cortex

    Joon-Young Moon, Kathrin Müsch ... Christopher J. Honey
    1. Neuroscience

    Implications of variable synaptic weights for rate and temporal coding of cerebellar outputs

    Shuting Wu, Asem Wardak ... Wade G Regehr
    Purkinje cell inputs onto cerebellar nuclei (CbN) neurons have highly variable strengths, which allow them to influence CbN firing in a graded manner, with the largest individual inputs effectively regulating both the rate and spike timing of CbN neuron firing.
    1. Physics of Living Systems

    Free volume theory explains the unusual behavior of viscosity in a non-confluent tissue during morphogenesis

    Rajsekhar Das, Sumit Sinha ... D Thirumalai
    Theoretical explanation and a novel mechanism are given for the observation that viscosity of embryonic non-confluent tissue shows glass-like behavior till a critical cell density and saturates at higher densities.
    1. Neuroscience

    Acetylcholine modulates the precision of prediction error in the auditory cortex

    David Pérez-González, Ana Belén Lao-Rodríguez ... Manuel S Malmierca
    Acetylcholine plays a multifold role in modulating neuronal mismatch in auditory cortex, affecting the precision of prediction error signaling and gating prediction errors to hierarchically higher processing levels.
    1. Genetics and Genomics

    Auxin exposure disrupts feeding behavior and fatty acid metabolism in adult Drosophila

    Sophie A Fleck, Puja Biswas ... Lesley N Weaver
    Adult Drosophila exposed to auxin at the recommended concentrations for the AGES transgene expression system display defects in feeding behavior, have altered metabolic transcripts, and have reduced triacylglyceride levels.
    1. Chromosomes and Gene Expression

    Regulation of chromatin architecture by transcription factor binding

    Stephanie Portillo-Ledesma, Suckwoo Chung ... Tamar Schlick
    The binding of transcription factors to mesoscale chromatin fibers leads to microdomains whose features are dependent on the linker DNA length, linker histone density, and tail acetylation levels.
    1. Computational and Systems Biology

    Analysis of NIH K99/R00 awards and the career progression of awardees

    Nicole C Woitowich, Sarah R Hengel ... Daniel J Tyrrell
    There is a significant disadvantage to receive a major grant (i.e., R01) for K99/R00 awardees that are women, those at lower funded institutions, and those with lower career mobility.
    1. Genetics and Genomics
    2. Medicine

    Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition

    Dylan C Sarver, Jean Garcia-Diaz ... G William Wong
    Mice lacking Tmem263 are dwarfs, and this phenotype is associated with reduced hepatic growth hormone (GH) receptor expression, a deficit in GH-induced signaling, and low insulin-like growth factor 1 level.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Genetic basis of Arabidopsis thaliana responses to infection by naïve and adapted isolates of turnip mosaic virus

    Anamarija Butkovic, Thomas James Ellis ... Santiago F Elena
    Arabidopsis lines were screened for resistance to TuMV, identifying a region on chromosome 2 linked to necrosis that includes an antiviral gene.
    1. Chromosomes and Gene Expression

    Monoallelically expressed noncoding RNAs form nucleolar territories on NOR-containing chromosomes and regulate rRNA expression

    Qinyu Hao, Minxue Liu ... Kannanganattu V Prasanth
    A novel family of nucleolus-enriched ncRNAs forms allele-specific territories on the nucleolar organizing region (NOR)-containing acrocentric chromosomes.
    1. Cell Biology

    Organelle proteomic profiling reveals lysosomal heterogeneity in association with longevity

    Yong Yu, Shihong M Gao ... Meng C Wang
    First systemic lysosomal proteomes in association with longevity and tissue specificity in C. elegans uncover lysosomal heterogeneity across multiple scales and provide valuable resources for understanding how lysosomes modulate signal transduction, organelle communication, tissue physiology, and organism longevity.
    1. Cell Biology

    Progesterone induces meiosis through two obligate co-receptors with PLA2 activity

    Nancy Nader, Lubna Zarif ... Khaled Machaca
    1. Medicine

    Hammerhead-type FXR agonists induce an eRNA FincoR that ameliorates nonalcoholic steatohepatitis in mice

    Jinjing Chen, Ruoyu Wang ... Jongsook Kim Kemper
    1. Neuroscience

    The breath shape controls intonation of mouse vocalizations

    Alastair MacDonald, Kevin Yackle
    1. Cell Biology

    N-cadherin mechanosensing in ovarian follicles controls oocyte maturation and ovulation

    Alaknanda Emery, Orest W. Blaschuk ... Darryl L Russell
    1. Developmental Biology

    Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill During Fertilization

    Martina Jabloñski, Guillermina M. Luque ... Mariano G. Buffone
    1. Genetics and Genomics
    2. Evolutionary Biology

    Random genetic drift sets an upper limit on mRNA splicing accuracy in metazoans

    Florian Bénitière, Anamaria Necsulea, Laurent Duret
    1. Neuroscience

    What happens to the inhibitory control functions of the right inferior frontal cortex when this area is dominant for language?

    Esteban Villar-Rodríguez, Cristina Cano-Melle ... César Avila
    A complementary relationship exists between the hemispheric lateralization of inhibitory control (typically right) and speech (typically left), as observed in typically and atypically lateralized left-handed individuals.
    1. Immunology and Inflammation

    Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens

    Munir Akkaya, Jafar Al Souz ... Billur Akkaya
    Novel antigen-targeting probes, featuring a cysteine-rich tag emitting fluorescence, offer unprecedented insight into antigen-specific immune responses.
    1. Chromosomes and Gene Expression

    Spatial chromatin accessibility sequencing resolves high-order spatial interactions of epigenomic markers

    Yeming Xie, Fengying Ruan ... Chong Tang
    SCA-seq enables simultaneous detection of spatial interactions, chromatin accessibility, and CpG methylation at single-molecule resolution, advancing multi-omics studies of genome spatial organization.
    1. Stem Cells and Regenerative Medicine

    An optogenetic cell therapy to restore control of target muscles in an aggressive mouse model of amyotrophic lateral sclerosis

    J Barney Bryson, Alexandra Kourgiantaki ... Linda Greensmith
    Stem-cell-based neural replacement, in combination with optogenetic stimulation, could represent a translationally viable therapeutic strategy to overcome atrophy and paralysis of targeted muscles in people living with amyotrophic lateral sclerosis.
    1. Neuroscience

    14-3-3 protein augments the protein stability of phosphorylated spastin and promotes the recovery of spinal cord injury through its agonist intervention

    Qiuling Liu, Hua Yang ... Zhisheng Ji
    14-3-3 protein augments the protein stability of phosphorylated spastin and promotes the recovery of spinal cord injury in mice.
    1. Evolutionary Biology

    Evolution of the gene regulatory network of body axis by enhancer hijacking in amphioxus

    Chenggang Shi, Shuang Chen ... Guang Li
    The gene regulatory network of Nodal signaling, underpinning body axes patterning, was evolved specifically in cephalochordate lineage.
    1. Immunology and Inflammation

    Catalytic activity and autoprocessing of murine caspase-11 mediate noncanonical inflammasome assembly in response to cytosolic LPS

    Daniel C Akuma, Kimberly A Wodzanowski ... Igor E Brodsky
    Caspase-11 catalytic activity and autoprocessing play key roles upstream rather than downstream of its assembly into higher-order inflammasome complexes in response to cytosolic bacterial lipopolysaccharide.
    1. Neuroscience

    Neuroendocrine gene expression coupling of interoceptive bacterial food cues to foraging behavior of C. elegans

    Sonia A Boor, Joshua D Meisel, Dennis H Kim
    Distinct interoceptive and gustatory bacterial food cues converge to control neuroendocrine gene expression in two neurons, which modulates and is correlated with internal states driving feeding versus foraging behavior.
    1. Neuroscience

    A generative model of electrophysiological brain responses to stimulation

    Diego Vidaurre
    Genephys is a generative model for dissecting the different aspects that compound our neural responses to perceptual stimulation, identifying which aspects remain stable and which ones vary across experimental repetitions.
    1. Neuroscience

    Interaction of human keratinocytes and nerve fiber terminals at the neuro-cutaneous unit

    Christoph Erbacher, Sebastian Britz ... Nurcan Üçeyler
    Sensory nerve fiber ending ensheathment and connexin 43 contacts by keratinocytes at the neuro-cutaneous unit can be visualized and quantified at super-resolution in human skin.
    1. Cell Biology

    Dual recognition of multiple signals in bacterial outer membrane proteins enhances assembly and maintains membrane integrity

    Edward M Germany, Nakajohn Thewasano ... Takuya Shiota
    Bacterial outer membrane proteins are recognized by BamD at specific signals located in multiple β-strands at the C-terminus of these proteins, and this recognition is important for efficient outer membrane protein assembly and maintaining the integrity of outer membrane.
    1. Developmental Biology

    Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling

    Steven A Cincotta, Nainoa Richardson ... Diana J Laird
    Although developing oocytes and sperm of mice both harbor the glucocorticoid receptor, fetal oocytes are resistant to differing levels of gluocorcorticoid stress hormones, while spermatogonia alter RNA splicing.
    1. Computational and Systems Biology

    Structural assembly of the bacterial essential interactome

    Jordi Gómez Borrego, Marc Torrent Burgas
    A new study reveals structural details of the bacterial essential interactome, opening new avenues for antibiotic discovery.
    1. Neuroscience

    EphrinB2 knockdown in cervical spinal cord preserves diaphragm innervation in a mutant SOD1 mouse model of ALS

    Mark W Urban, Brittany A Charsar ... Angelo C Lepore
    In the SOD1G93A mouse model of amyotrophic lateral sclerosis (ALS), expression of transmembrane signaling molecule ephrinB2 in spinal cord astrocytes contributes to motor neuron damage and loss of diaphragm function.
    1. Neuroscience

    Resource-rational account of sequential effects in human prediction

    Arthur Prat-Carrabin, Florent Meyniel, Rava Azeredo da Silveira
    A proposed model of optimal inference under cognitive costs accounts for human sequential effects, including subtle patterns of attractive and repulsive influence of past observations, in a binary prediction task across a wide range of stimulus conditions.
    1. Genetics and Genomics

    Leveraging inter-individual transcriptional correlation structure to infer discrete signaling mechanisms across metabolic tissues

    Mingqi Zhou, Ian Tamburini ... Marcus M Seldin
    A population resource to investigate mechanisms of organ communication.
    1. Medicine
    2. Neuroscience

    Resting-state alterations in behavioral variant frontotemporal dementia are related to the distribution of monoamine and GABA neurotransmitter systems

    Lisa Hahn, Simon B Eickhoff ... Matthias L Schroeter
    Local brain functional activity reductions in bvFTD follow the distribution of serotonergic, GABAergic, and norepinephrinergic neurotransmitter systems, indicating a selective vulnerability and providing novel insights into the underlying disease mechanisms.
    1. Epidemiology and Global Health

    Design of the HPV-automated visual evaluation (PAVE) study: Validating a novel cervical screening strategy

    Silvia de Sanjosé, Rebecca B Perkins ... Mark Schiffman
    The PAVE strategy aims to provide a cost-effective, high quality ‘Screen-Triage-Treat’ approach for cervical cancer screening to save many lives.
    1. Neuroscience

    Toolkits for detailed and high-throughput interrogation of synapses in C. elegans

    Maryam Majeed, Haejun Han ... Hang Lu
    An imaging pipeline together with a collection of transgenic nematode strains provide a strategy to visualize and properly quantify synaptic connectivity in the C. elegans brain.
    1. Genetics and Genomics

    Multi-ancestry meta-analysis of host genetic susceptibility to tuberculosis identifies shared genetic architecture

    Haiko Schurz, Vivek Naranbhai ... International Tuberculosis Host Genetics Consortium
    Results from the largest tuberculosis genome-wide association study meta-analysis identified a significant association in the human leukocyte antigen class II region, rs28383206, across multiple populations.
    1. Microbiology and Infectious Disease

    Dynamics of macrophage polarization support Salmonella persistence in a whole living organism

    Jade Leiba, Tamara Sipka ... Mai E Nguyen-Chi
    Real-time visualization of Salmonella enterica and polarized macrophage interaction in the living host identifies an anti-inflammatory, pro-regenerative, and motionless macrophage subset as a survival niche during persistent infection.
    1. Cell Biology
    2. Physics of Living Systems

    Probe-free optical chromatin deformation and measurement of differential mechanical properties in the nucleus

    Benjamin Seelbinder, Susan Wagner ... Moritz Kreysing
    Localized temperature gradients used as a novel method of active micro-theology that is non-invasive and probe-free, to investigate material responses of cell departments such as the different compartments of a nucleus displaying distinct material properties.
    1. Neuroscience

    A preclinical model of THC edibles that produces high-dose cannabimimetic responses

    Anthony English, Fleur Uittenbogaard ... Benjamin Bruce Land
    Δ9-Tetrahydrocannabinol (THC)-E-gel enables robust preclinical study of voluntary THC consumption in mice, revealing sex-dependent psychotomimetic effects that are valuable for understanding cannabimimetic responses.
    1. Structural Biology and Molecular Biophysics

    Conformational dynamics of a nicotinic receptor neurotransmitter binding site

    Mrityunjay Singh, Dinesh C. Indurthi ... Shailendra Asthana
    1. Genetics and Genomics

    PARP-1 cooperates with histone methyltransferase to regulate transcription

    Gbolahan Bamgbose, Guillaume Bordet ... Alexei Tulin
    1. Microbiology and Infectious Disease
    2. Structural Biology and Molecular Biophysics

    A Vibrio cholerae viral satellite maximizes its spread and inhibits phage by remodeling hijacked phage coat proteins into small capsids

    Caroline M Boyd, Sundharraman Subramanian ... Kimberley D Seed
    A phage parasite encodes an external scaffolding protein to pirate and rearrange phage-encoded coat proteins to more efficiently transfer the phage parasite genome to new hosts and limit phage production.
    1. Computational and Systems Biology

    Long-read single-cell sequencing reveals expressions of hypermutation clusters of isoforms in human liver cancer cells

    Silvia Liu, Yan-Ping Yu ... Jian-Hua Luo
    A synthetic long-read single-cell sequencing reveals critical mutation isoform expression patterns in human liver cancer cells.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Comparative analysis of two Caenorhabditis elegans kinesins KLP-6 and UNC-104 reveals a common and distinct activation mechanism in kinesin-3

    Tomoki Kita, Kyoko Chiba ... Shinsuke Niwa
    Comparative biochemical analysis of a nematode-specific kinesin-3 shed light on the mechanism of kinesin-3 dimerization and activation.
    1. Cell Biology

    Location, location, location: Protein kinase nanoclustering for optimised signalling output

    Rachel S Gormal, Ramon Martinez-Marmol ... Frédéric A Meunier
    This article reviews the latest studies that have used super-resolution microscopy and cluster analysis methodologies to study the mechanism of protein kinase signalling hubs and their organisation at nanoscale.
    1. Structural Biology and Molecular Biophysics

    Structure of the human heparan-α-glucosaminide N-acetyltransferase (HGSNAT)

    Vikas Navratna, Arvind Kumar, Shyamal Mosalaganti
    1. Neuroscience

    Lesions in a songbird vocal circuit increase variability in song syntax

    Avani Koparkar, Timothy L. Warren ... Lena Veit
    1. Neuroscience

    Visual experience shapes functional connectivity between occipital and non-visual networks

    Mengyu Tian, Xiang Xiao ... Marina Bedny
    1. Physics of Living Systems

    Gendered hiring and attrition on the path to parity for academic faculty

    Nicholas LaBerge, K. Hunter Wapman ... Daniel B. Larremore
    1. Cell Biology
    2. Computational and Systems Biology

    circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA

    Trine Line Hauge Okholm, Andreas Bjerregaard Kamstrup ... Christian Kroun Damgaard
    1. Neuroscience

    Early roots of information-seeking: Infants predict and generalize the value of information

    Tommaso Ghilardi, Francesco Poli ... Sabine Hunnius
    1. Neuroscience

    When Abstract Becomes Concrete: Naturalistic Encoding of Concepts in the Brain

    Viktor Kewenig, Gabriella Vigliocco, Jeremy I Skipper
    1. Neuroscience

    The Na+ leak channel NALCN controls spontaneous activity and mediates synaptic modulation by α2-adrenergic receptors in auditory neurons

    Tenzin Ngodup, Tomohiko Irie ... Laurence O Trussell
    Noradrenergic receptors can control the spontaneous electrical firing of neurons by inhibiting a Na+ channel known as NALCN using a pathway shared by metabotropic GABA receptors.
    1. Chromosomes and Gene Expression

    A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination

    Salman Shehzada, Tomoko Noto ... Kazufumi Mochizuki
    The identification of a SUMO E3 ligase engaged in lncRNA transcription, subsequently leading to target-directed small RNA degradation, reveals an unexplored layer of regulatory mechanisms within small RNA-directed chromatin regulation.
    1. Computational and Systems Biology

    Affected cell types for hundreds of Mendelian diseases revealed by analysis of human and mouse single-cell data

    Idan Hekselman, Assaf Vital ... Esti Yeger-Lotem
    The PrEDiCT scheme infers disease-affected cell types from the expression of disease-associated genes in single-cell expression atlases, thereby providing cellular context and enhancing mechanistic understanding for 328 Mendelian diseases.
    1. Developmental Biology

    Notch signaling and Bsh homeodomain activity are integrated to diversify Drosophila lamina neuron types

    Chundi Xu, Tyler B Ramos ... Chris Q Doe
    Notch signaling specifies binary neuronal fates through its integration with primary homeodomain activity.
    1. Biochemistry and Chemical Biology
    2. Stem Cells and Regenerative Medicine

    BRAIDing receptors for cell-specific targeting

    Hui Chen, Sung-Jin Lee ... Yang Li
    A novel strategy to activate the WNT pathway in a cell type selective manner by linking designer ligands with target cell specific bridging receptors.
    1. Immunology and Inflammation

    Ym1 protein crystals promote type 2 immunity

    Ines Heyndrickx, Kim Deswarte ... Bart N Lambrecht
    Protein crystallization is a rare event yet Ym1 crystals made from a chitinase-like protein are abundantly found in eosinophilic airway inflammation, where they promote type 2 immunity.
    1. Neuroscience

    MTL neurons phase-lock to human hippocampal theta

    Daniel R Schonhaut, Aditya M Rao ... Michael J Kahana
    1. Ecology

    Role of immigrant males and muzzle contacts in the uptake of a novel food by wild vervet monkeys

    Pooja Dongre, Gaëlle Lanté ... Erica van de Waal
    New immigrants triggered fast uptake of a novel food in groupmates, who obtained information about it through muzzle contacts, opening up new questions about the role of dispersers in information flow around a population.
    1. Neuroscience

    Experience shapes chandelier cell function and structure in the visual cortex

    Koen Seignette, Nora Jamann ... Christiaan N Levelt
    Chandelier cells in primary visual cortex show experience-dependent plasticity, respond strongly to behaviorally relevant stimuli and only weakly inhibit pyramidal cells.
    1. Neuroscience

    A translational MRI approach to validate acute axonal damage detection as an early event in multiple sclerosis

    Antonio Cerdán Cerdá, Nicola Toschi ... Silvia De Santis
    MRI is sensitive to the increase in axonal caliber due to pathology and was used to detect widespread increase in the average axonal caliber in multiple sclerosis brains with short disease duration only.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    The infection-tolerant white-footed deermouse tempers interferon responses to endotoxin in comparison to the mouse and rat

    Ana Milovic, Jonathan V Duong, Alan G Barbour
    Comparing the white-footed deermouse with mice and rats in an inflammation model reveals a means for the observed infection tolerance in this key animal reservoir for several human diseases.
    1. Ecology
    2. Evolutionary Biology

    Fin whale song evolution in the North Atlantic

    Miriam Romagosa, Sharon Nieukirk ... Mónica A Silva
    The rapid and gradual fin whale songs changes adopted by individuals of a certain area provide evidence of vocal learning in this species, elucidate patterns of song evolution and hints on the limits of song variation.
    1. Cell Biology

    ARHGAP18-ezrin functions as an autoregulatory module for RhoA in the assembly of distinct actin-based structures

    Andrew T Lombardo, Cameron AR Mitchell ... Anthony Bretscher
    ARHGAP18 specializes in controlling cell shape at the topmost layer of the cell membrane by regulating the interaction between the ERM protein family, RhoA signaling, and the actin cytoskeleton.
    1. Neuroscience

    Balance of activity during a critical period tunes a developing network

    Iain Hunter, Bramwell Coulson ... Richard A Baines
    Neurodevelopmental critical periods act to integrate activity to encode homeostatic set points that remain fixed thereafter.
    1. Developmental Biology

    Intrinsic and extrinsic cues time somite progenitor contribution to the vertebrate primary body axis

    Lara Busby, Guillermo Serrano Nájera, Benjamin John Steventon
    Cells know when to contribute to the maturing embryonic body axis by adjusting an internal timer to the conditions around them.
    1. Neuroscience

    Unraveling the developmental dynamic of visual exploration of social interactions in autism

    Nada Kojovic, Sezen Cekic ... Marie Schaer
    Children with ASD showed a significant divergence in gaze patterns compared to typically developing children, intensifying over early childhood, with implications for developmental and adaptive functioning.
    1. Microbiology and Infectious Disease

    SPARK regulates AGC kinases central to the Toxoplasma gondii asexual cycle

    Alice L. Herneisen, Michelle L. Peters ... Sebastian Lourido
    1. Cell Biology

    Cohesin still drives homologous recombination repair of DNA double-strand breaks in late mitosis

    Jessel Ayra-Plasencia, Lorraine Symington, Félix Machín
    1. Cell Biology
    2. Microbiology and Infectious Disease

    A conserved cell-pole determinant organizes proper polar flagellum formation

    Erick Eligio Arroyo-Pérez, John C. Hook ... Simon Ringgaard
    1. Medicine

    Human HPSE2 gene transfer ameliorates bladder pathophysiology in a mutant mouse model of urofacial syndrome

    Filipa M. Lopes, Celine Grenier ... Neil A. Roberts
    1. Neuroscience

    Pain persists in mice lacking both Substance P and CGRPα signaling

    Donald Iain MacDonald, Monessha Jayabalan ... Alexander Chesler
    1. Cell Biology
    2. Medicine

    Chemotherapy activates inflammasomes to cause inflammation-associated bone loss

    Chun Wang, Khushpreet Kaur ... Gabriel Mbalaviele
    1. Evolutionary Biology
    2. Genetics and Genomics

    Separating phases of allopolyploid evolution with resynthesized and natural Capsella bursa-pastoris

    Tianlin Duan, Adrien Sicard ... Martin Lascoux
    A comparison between artificially created allopolyploids and natural ones reveals that the latter underwent significant evolutionary steps after their formation.
    1. Biochemistry and Chemical Biology
    2. Ecology

    Molecular mechanisms of microbiome modulation by the eukaryotic secondary metabolite azelaic acid

    Ahmed A Shibl, Michael A Ochsenkühn ... Shady A Amin
    Characterization of the molecular mechanisms that enable azelaic acid, a secondary metabolite produced by photosynthetic organisms, to promote or inhibit different bacterial populations.
    1. Physics of Living Systems

    Hydrodynamics and multiscale orderin confluent epithelia

    Josep-Maria Armengol-Collado, Livio Nicola Carenza, Luca Giomi
    1. Structural Biology and Molecular Biophysics

    Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states

    David Yin-wei Lin, Lauren E Kueffer ... Amy H Andreotti
    Multiple dynamic protein interactions converge to regulate the catalytic activity of the B cell kinase, BTK.
    1. Chromosomes and Gene Expression

    Gle1 is required for tRNA to stimulate Dbp5 ATPase activity in vitro and promote Dbp5-mediated tRNA export in vivo in Saccharomyces cerevisiae

    Arvind Arul Nambi Rajan, Ryuta Asada, Ben Montpetit
    In vivo and in vitro characterization demonstrates a direct interaction of Dbp5 with tRNA that requires Gle1 to spatially activate the Dbp5 ATPase cycle for tRNA export in Saccharomyces cerevisiae.
    1. Cell Biology
    2. Immunology and Inflammation

    DNA damage signaling in Drosophila macrophages modulates systemic cytokine levels in response to oxidative stress

    Fabian Hersperger, Tim Meyring ... Katrin Kierdorf
    Immune activation in Drosophila macrophages during oxidative stress is regulated by DNA damage signaling, which controls proinflammatory cytokine release and the susceptibility of the fly to oxidative stress.
    1. Evolutionary Biology

    A scenario for an evolutionary selection of ageing

    T. Roget, C. MacMurray ... M. Rera
    1. Neuroscience
    2. Evolutionary Biology

    Comparative neuroimaging of sex differences in human and mouse brain anatomy

    Elisa Guma, Antoine Beauchamp ... Jason P Lerch
    1. Developmental Biology

    Homeodomain proteins hierarchically specify neuronal diversity and synaptic connectivity

    Chundi Xu, Tyler B Ramos ... Chris Q Doe
    The homeodomain protein Bsh increases lamina neuron types and visual function.
    1. Neuroscience

    Acute stress reduces effortful prosocial behaviour

    Paul AG Forbes, Gökhan Aydogan ... Claus Lamm
    Participants under acute stress were less willing to exert a relatively low level of physical effort for actions that benefit another person compared to actions that benefit themselves.
    1. Neuroscience

    A maximum of two readily releasable vesicles per docking site at a cerebellar single active zone synapse

    Melissa Silva, Van Tran, Alain Marty
    Counting the number of synaptic vesicles released in simple synapses under high release probability conditions revealed a maximum readily releasable pool size of two vesicles per docking site.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Regulation of multiple signaling pathways promotes the consistent expansion of human pancreatic progenitors in defined conditions

    Luka Jarc, Manuj Bandral ... Anthony Gavalas
    The signaling requirements to decouple proliferation of pancreatic progenitors from differentiation were elucidated and employed for the reproducible expansion, under GMP-compliant conditions, of pancreatic progenitors derived from different human pluripotent stem cell lines.
    1. Structural Biology and Molecular Biophysics

    Structural and mechanistic insights into ribosomal ITS2 RNA processing by nuclease-kinase machinery

    Jiyun Chen, Hong Chen ... Liang Liu
    The structures of full-length Las1-Grc3 complexes provide crucial insights into the activation mechanism of HEPN nuclease and the pathway of pre-rRNA processing in ribosome biosynthesis.
    1. Genetics and Genomics
    2. Medicine

    Proteomic analysis shows decreased type I fibers and ectopic fat accumulation in skeletal muscle from women with PCOS

    Elisabet Stener-Victorin, Gustaw Eriksson ... Anna Benrick
    Elevated levels of ectopic fat and a decreased number of oxidative insulin-sensitive type I muscle fibers in skeletal muscle could lead to insulin resistance in women with polycystic ovary syndrome.
    1. Neuroscience

    Inhibitory CCK+ basket synapse defects in mouse models of dystroglycanopathy

    Jennifer N Jahncke, Daniel S Miller ... Kevin M Wright
    Loss of functional dystroglycan disrupts the formation and function of CCK+/CB1R+ inhibitory synapses in hippocampal CA1, resulting in reduced seizure thresholds in mouse models of dystroglycanopathy.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Interface-acting nucleotide controls polymerization dynamics at microtubule plus- and minus-ends

    Lauren A McCormick, Joseph M Cleary ... Luke M Rice
    Comparative measurements and simulations of microtubule plus and minus end growth with mixed nucleotides demonstrate that nucleotide acts across a tubulin:tubulin interface to influence microtubule stability and dynamics.
    1. Neuroscience
    2. Physics of Living Systems

    Criticality supports cross-frequency cortical-thalamic information transfer during conscious states

    Daniel Toker, Eli Müller ... Martin M Monti
    Cross-frequency communication between the cortex and thalamus is linked to consciousness and changes in unconscious and psychedelic states, possibly due to shifts in brain dynamics between stability and chaos.
    1. Cell Biology

    Involvement of TRPV4 in temperature-dependent perspiration in mice

    Makiko Kashio, Sandra Derouiche ... Makoto Tominaga
    1. Neuroscience

    Cell type-specific connectome predicts distributed working memory activity in the mouse brain

    Xingyu Ding, Sean Froudist-Walsh ... Xiao-Jing Wang
    Large-scale modeling of the mouse brain revealed that working memory is modular and determined by long-range cell type-specific connections, thereby providing a framework to interpret brain-wide recordings during cognition.
    1. Neuroscience

    Space as a Scaffold for Rotational Generalisation of Abstract Concepts

    Jacques Pesnot Lerousseau, Christopher Summerfield
    1. Biochemistry and Chemical Biology
    2. Neuroscience

    Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques

    Charlotte Rimbault, Christelle Breillat ... Matthieu Sainlos
    Engineering new intrabodies allows to develop selective and non-interfering tools for the visualization of endogenous PSD-95 with advanced imaging techniques.
    1. Immunology and Inflammation
    2. Structural Biology and Molecular Biophysics

    Asymmetric framework motion of TCRαβ controls load-dependent peptide discrimination

    Ana C Chang-Gonzalez, Robert J Mallis ... Wonmuk Hwang
    All-atom molecular dynamics simulations of an αβ T-cell receptor complexed with the major histocompatibility complex molecule presenting wild-type or mutant antigenic peptides reveal how it uses conserved framework motion to discriminate antigens by leveraging physiological force applied during immune surveillance.
    1. Stem Cells and Regenerative Medicine

    Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells

    Matias I Autio, Efthymios Motakis ... Roger SY Foo
    Depiction of novel human genomic safe harbour loci that have been validated in vitro for safety and stable expression in human embryonic stem cells and cell types from all the three germ lineages.
    1. Developmental Biology
    2. Neuroscience

    RNA fusion in human retinal development

    Wen Wang, Xiao Zhang ... Zi-Bing Jin
    Chimeric RNAs are widely distributed spatiotemporally during human retinal development and have important regulatory functions, such as silencing of CTNNBIP1-CLSTN1 biasing the progenitor cells toward the RPE cell fate at the expense of neural retinal cell fates.