January 2024

Research articles

1. 1. Cell Biology
   2. Neuroscience

   Cardiac glycosides restore autophagy flux in an iPSC-derived neuronal model of WDR45 deficiency

   Apostolos Papandreou, Nivedita Singh ... Robin Ketteler
2. 1. Plant Biology

   Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet

   Carla de la Fuente, Alexandre Grondin ... Laurent Laplaze

   Increased primary root growth in pearl millet is correlated with early water stress tolerance, an important constraint in agrosystems in the Sahel, and is associated with the regulation of root cell elongation by a glutaredoxin.
3. 1. Microbiology and Infectious Disease

   A dynamic bactofilin cytoskeleton cooperates with an M23 endopeptidase to control bacterial morphogenesis

   Sebastian Pöhl, Manuel Osorio-Valeriano ... Martin Thanbichler

   Analyses of the stalked budding bacterium Hyphomonas neptunium and its spiral-shaped relative Rhodospirillum rubrum reveal a conserved morphogenetic module that controls the establishment of complex bacterial cell shapes.
4. 1. Physics of Living Systems

   The ability to sense the environment is heterogeneously distributed in cell populations

   Andrew Goetz, Hoda Akl, Purushottam Dixit

   Information transduction capacity of mammalian cells is high and varies substantially from cell to cell.
5. 1. Computational and Systems Biology

   Detection of new pioneer transcription factors as cell-type-specific nucleosome binders

   Yunhui Peng, Wei Song ... Anna R Panchenko

   A computational method has been developed for predicting the cell-type-specific binding of transcription factors to nucleosomes, and involves integration of ChIP-seq, MNase-seq, and DNase-seq data with details of nucleosome structure.
6. 1. Cell Biology

   CEP44 is required for maintaining centriole duplication and spindle integrity

   Donghui Zhang, Wenlu Wei ... Jianguo Chen
7. 1. Medicine

   Loss of CTRP10 results in female obesity with preserved metabolic health

   Fangluo Chen, Dylan C. Sarver ... G. William Wong
8. 1. Cell Biology

   Autoacetylation-mediated phase separation of TIP60 is critical for its functions

   Shraddha Dubey, Himanshu Gupta, Ashish Gupta
9. 1. Cancer Biology

   Targeting the Hippo pathway in cancers via ubiquitination dependent TEAD degradation

   Trang H. Pham, Kanika Bajaj Pahuja ... Anwesha Dey
10. 1. Cell Biology
    2. Neuroscience

    Aβ-driven nuclear pore complex dysfunction alters activation of necroptosis proteins in a mouse model of Alzheimer’s Disease

    Vibhavari Aysha Bansal, Jia Min Tan ... Toh Hean Ch’ng
11. 1. Neuroscience

    SRF-deficient astrocytes provide neuroprotection in mouse models of excitotoxicity and neurodegeneration

    Surya Chandra Rao Thumu, Monika Jain ... Narendrakumar Ramanan

    Serum response factor (SRF) deficient reactive astrocytes are neuroprotective in the mammalian brain.
12. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Heat stress-induced activation of MAPK pathway attenuates Atf1-dependent epigenetic inheritance of heterochromatin in fission yeast

    Li Sun, Libo Liu ... Quan-wen Jin

    Fission yeast loses its heterochromatin stability at mating-type locus under high temperature due to compromised binding between heterochromatin protein Swi6^HP1 and Atf1.
13. 1. Evolutionary Biology
    2. Genetics and Genomics

    Stable population structure in Europe since the Iron Age, despite high mobility

    Margaret L Antonio, Clemens L Weiß ... Jonathan K Pritchard

    Reconstructing human mobility patterns using historical period genomes illustrates how the Roman Empire’s military and economic activities catalyzed an era of transient movement against the backdrop of generations-long prehistoric migrations.
14. 1. Structural Biology and Molecular Biophysics

    Discovery of lipid binding sites in a ligand-gated ion channel by integrating simulations and cryo-EM

    Cathrine Bergh, Urška Rovšnik ... Erik Lindahl

    A combination of molecular dynamics simulations and electron cryomicroscopy characterizes interactions of a pentameric ligand-gated ion channel with 25 lipid molecules, specifically in a functional state in which they were not previously observed.
15. 1. Neuroscience

    Coupling of Slack and Na_V1.6 sensitizes Slack to quinidine blockade and guides anti-seizure strategy development

    Tian Yuan, Yifan Wang ... Zhuo Huang

    Unveiling Na_V1.6's role in sensitizing Slack to quinidine disruption redefines KCNT1-related epilepsy treatment strategies.
16. 1. Neuroscience

    Behavioral entrainment to rhythmic auditory stimulation can be modulated by tACS depending on the electrical stimulation field properties

    Yuranny Cabral-Calderin, Daniela van Hinsberg ... Molly J Henry

    Transcranial alternating current stimulation modulates behavioral entrainment to sounds.
17. 1. Cell Biology
    2. Developmental Biology

    Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function

    Chao Chang, Sara L Banerjee ... Artur Kania

    Extracellular signals mediated by the EphB2 receptor tyrosine kinase may access the effectors of fundamental cell functions controlled by MYCBP2.
18. 1. Genetics and Genomics
    2. Neuroscience

    Orai-mediated calcium entry determines activity of central dopaminergic neurons by regulation of gene expression

    Rishav Mitra, Shlesha Richhariya, Gaiti Hasan

    Expression of the appropriate repertoire of membrane channels, essential for activity of flight-promoting central dopaminergic neurons of Drosophila, requires store-operated Ca²⁺ entry during late pupal development and early adults.
19. 1. Genetics and Genomics

    Targeting sex determination to suppress mosquito populations

    Ming Li, Nikolay P Kandul ... Omar S Akbari

    Genetic analyses and mathematical modeling reveal that disrupting genes essential for sex determination and fertility in Aedes aegypti leads to the production of sterile males, effectively suppressing mosquito populations.
20. 1. Structural Biology and Molecular Biophysics

    Explicit ion modeling predicts physicochemical interactions for chromatin organization

    Xingcheng Lin, Bin Zhang

    The physicochemical interactions among wild-type nucleosomes hold substantial significance and play a role in chromatin folding under physiological salt concentrations.
21. 1. Neuroscience

    Spatial transcriptomics and single-nucleus RNA sequencing reveal a transcriptomic atlas of adult human spinal cord

    Donghang Zhang, Yali Chen ... Cheng Zhou

    The molecular, cellular, and spatial heterogeneity of adult human spinal cord serve as an important resource to explore the mechanisms underlying spinal cord physiology and diseases.
22. 1. Evolutionary Biology

    Analyses of allele age and fitness impact reveal human beneficial alleles to be older than neutral controls

    Alyssa M. Pivirotto, Alexander Platt ... Jody Hey
23. 1. Plant Biology

    The tRNA thiolation-mediated translational control is essential for plant immunity

    Xueao Zheng, Hanchen Chen ... Shunping Yan

    The transfer RNA thiolation modification is required for the efficient translation of the master immune regulator NPR1 in plants.
24. 1. Epidemiology and Global Health

    Region of Attainable Redaction, an extension of Ellipse of Insignificance analysis for gauging impacts of data redaction in dichotomous outcome trials

    David Robert Grimes

    Region of Attainable Redaction analysis allows scientists to estimate the potential impacts of redaction in biomedical science, helping to uncover potentially spurious or non-robust results.
25. 1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Ebola virus sequesters IRF3 in viral inclusion bodies to evade host antiviral immunity

    Lin Zhu, Jing Jin ... Cheng Cao

    Ebola virus VP35 protein evades host antiviral immunity by interacting with STING to sequester IRF3 into inclusion bodies and inhibit type I interferon production.
26. 1. Developmental Biology

    Gene regulatory patterning codes in early cell fate specification of the C. elegans embryo

    Alison G Cole, Tamar Hashimshony ... Itai Yanai

    The transcriptomes of individual cells of the 1- to 102-cell stages in Caenorhabditis elegans embryogenesis are identified along with 119 embryonic cell states during cell fate specification, including ‘equivalence-group’ cell identities.
27. 1. Cell Biology
    2. Neuroscience

    Downregulation of Dickkopf-3, a Wnt antagonist elevated in Alzheimer’s disease, restores synapse integrity and memory in a disease mouse model

    Nuria Martin Flores, Marina Podpolny ... Patricia C Salinas

    The Wnt antagonist DKK3 is a key regulator of excitatory and inhibitory synapses, and its downregulation in the hippocampus restores synaptic connectivity and memory in an Alzheimer's disease mouse model.
28. 1. Neuroscience

    osl-dynamics, a toolbox for modeling fast dynamic brain activity

    Chetan Gohil, Rukuang Huang ... Mark W Woolrich

    A generative-model-based, unsupervised learning toolbox for characterizing oscillatory bursting and brain network dynamics in univariate or multivariate time series.
29. 1. Neuroscience

    Insights in neuronal tuning: Navigating the statistical challenges of autocorrelation and missing variables

    Fredrik Nevjen, Benjamin Adric Dunn
30. 1. Immunology and Inflammation

    Variation in the basal immune state and implications for disease

    Aisha Souquette, Paul G Thomas

    An integrative conceptual and mathematical framework of immune variation that includes unique basal profiles associated with differential susceptibility to disease, distinct acute immune signatures associated with disease severity, and how different determinants can lead to individual pathways of illness outcome.
31. 1. Developmental Biology

    Coordination of cell cycle and morphogenesis during organ formation

    Jeffrey Matthew, Vishakha Vishwakarma ... SeYeon Chung

    The SP1/KLF transcription factor Huckebein controls endoreplication in the Drosophila salivary gland, ensuring proper morphogenesis during organ formation.
32. 1. Chromosomes and Gene Expression
    2. Evolutionary Biology

    Tempo and mode of gene expression evolution in the brain across primates

    Katherine Rickelton, Trisha M Zintel ... Courtney C Babbitt

    Larger phylogenetic context is required to understand human and nonhuman primate brain evolution.
33. 1. Genetics and Genomics

    Association of genetic variation in COL11A1 with adolescent idiopathic scoliosis

    Hao Yu, Anas M Khanshour ... Carol A Wise

    Variation in type XI collagen alters estrogen-dependent signaling in the extracellular matrix and is identified as a genetic risk factor for adolescent idiopathic scoliosis, a common childhood deformity.
34. 1. Chromosomes and Gene Expression

    Intrinsic protein disorder is insufficient to drive subnuclear clustering in embryonic transcription factors

    Colleen E Hannon, Michael B Eisen

    A broad, live imaging-based survey of intrinsic protein disorder in Drosophila transcription factors reveals a limited role for their contribution to the protein clustering observed at enhancers.
35. 1. Cell Biology

    Calcium transients trigger switch-like discharge of prostaglandin E₂ in an extracellular signal-regulated kinase-dependent manner

    Tetsuya Watabe, Shinya Yamahira ... Kenta Terai

    Prostaglandin E₂-mediated radial spread of PKA activation, caused by calcium transients, is a novel mode of intercellular communication in living mice.
36. 1. Neuroscience
    2. Computational and Systems Biology

    Gain neuromodulation mediates perceptual switches: evidence from pupillometry, fMRI, and RNN Modelling

    Gabriel Wainstein, Christopher J. Whyte ... James M. Shine
37. 1. Immunology and Inflammation
    2. Cancer Biology

    Systematic evaluation of intratumoral and peripheral BCR repertoires in three cancers

    Sofia V Krasik, Ekaterina A Bryushkova ... Ekaterina O Serebrovskaya
38. 1. Computational and Systems Biology
    2. Physics of Living Systems

    Exploiting fluctuations in gene expression to detect causal interactions between genes

    Euan Joly-Smith, Mir Mikdad Talpur ... Andreas Hilfinger
39. 1. Ecology

    Automating an insect biodiversity metric using distributed optical sensors: an evaluation across Kansas, USA cropping systems

    Klas Rydhmer, James O Eckberg ... Emily N Bick
40. 1. Cell Biology

    Apical annuli are specialised sites of post-invasion secretion of dense granules in Toxoplasma

    Sara Chelaghma, Huiling Ke ... Ross F Waller

    Apicomplexan parasites secrete proteins to manipulate their hosts from sub-apical openings in their cell pellicle that are distinct from the apical complex from where invasion factors are secreted.
41. 1. Immunology and Inflammation

    Kidins220 regulates the development of B cells bearing the λ light chain

    Anna-Maria Schaffer, Gina Jasmin Fiala ... Susana Minguet

    The scaffold protein Kidins220 regulates the development of λLC B cells by supporting B cell precursor survival and optimizing pre‑BCR and BCR signaling.
42. 1. Neuroscience

    Involvement of superior colliculus in complex figure detection of mice

    J Leonie Cazemier, Robin Haak ... J Alexander Heimel

    Mouse superior colliculus is involved in figure detection based on differences in contrast and texture orientation and phase.
43. 1. Neuroscience

    A system of feed-forward cerebellar circuits that extend and diversify sensory signaling

    Harsh N Hariani, A Brynn Algstam ... Timothy S Balmer

    Unipolar brush cells subtypes, classified by their excitatory or inhibitory response to glutamate, form circuits with one another that may enhance the capacity of the cerebellum to transform input signals.
44. 1. Computational and Systems Biology
    2. Genetics and Genomics

    Comparative interactome analysis of α-arrestin families in human and Drosophila

    Kyung-Tae Lee, Inez KA Pranoto ... Jin-Wu Nam

    Unveiling the high-confidence protein–protein interaction networks of human and fly α-arrestins provides insights into conserved and species-specific functional roles depending on their protein domains and motifs.
45. 1. Cell Biology
    2. Developmental Biology

    Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice

    Longjie Sun, Zheng Lv ... Jiali Liu

    SRSF1 plays a critical role in posttranscriptional regulation to implement homing of precursor spermatogonial stem cells.
46. 1. Computational and Systems Biology
    2. Microbiology and Infectious Disease

    Light-inducible protein degradation in E. coli with the LOVdeg tag

    Nathan Tague, Cristian Coriano-Ortiz ... Mary J Dunlop

    The LOVdeg tag is a versatile tool for bacterial optogenetics, offering modular, blue light-inducible protein degradation for a range of synthetic biology and metabolic engineering applications.
47. 1. Microbiology and Infectious Disease

    A novel computational pipeline for var gene expression augments the discovery of changes in the Plasmodium falciparum transcriptome during transition from in vivo to short-term in vitro culture

    Clare Andradi-Brown, Jan Stephan Wichers-Misterek ... Anna Bachmann

    An enhanced bioinformatic pipeline to quantify Plasmodium falciparum core gene and polymorphic var gene expression revealed changes occurring during early culture adaptation of parasites from naturally infected individuals.
48. 1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Exploring the K⁺ binding site and its coupling to transport in the neurotransmitter:sodium symporter LeuT

    Solveig G Schmidt, Andreas Nygaard ... Claus J Loland

    Potassium presumably binds to the Na1 site in LeuT, playing a role in inwardly rectifying the transport of substrates.
49. 1. Neuroscience

    New genetic tools for mushroom body output neurons in Drosophila

    Gerald M Rubin, Yoshinori Aso

    Improved cell-type-specific genetic drivers for the study of learning and memory and other complex behaviors in Drosophila are provided and validated.
50. 1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    The bile acid receptor TGR5 regulates the hematopoietic support capacity of the bone marrow niche

    Alejandro Alonso-Calleja, Alessia Perino ... Olaia Naveiras
51. 1. Cell Biology
    2. Cancer Biology

    TIPE drives a cancer stem-like phenotype by promoting glycolysis via PKM2/HIF-1α axis in melanoma

    Maojin Tian, Le Yang ... Peiqing Zhao
52. 1. Developmental Biology

    Sperm induction of somatic cell-cell fusion as a novel functional test

    Nicolas G Brukman, Clari Valansi, Benjamin Podbilewicz

    The fertilizing potential of sperm correlates with their ability to induce viral-like fusion of somatic cells expressing JUNO.
53. 1. Cell Biology
    2. Developmental Biology

    Dally is not essential for Dpp spreading or internalization but for Dpp stability by antagonizing Tkv-mediated Dpp internalization

    Niklas Simon, Abu Safyan ... Shinya Matsuda

    The heparan sulfate chains of the glypican Dally stabilizes morphogen Dpp on the cell surface by antagonizing receptor-mediated Dpp internalization largely independent of direct interaction with Dpp.
54. 1. Genetics and Genomics
    2. Neuroscience

    The gene expression landscape of the human locus coeruleus revealed by single-nucleus and spatially-resolved transcriptomics

    Lukas M Weber, Heena R Divecha ... Stephanie C Hicks

    Spatially-resolved transcriptomics and single-nucleus RNA-sequencing are applied to the human locus coeruleus to characterize the gene expression profiles of norepinephrine (NE) neurons and other cell populations in this critical brain region, with all data made publicly available.
55. 1. Neuroscience

    Passive exposure to task-relevant stimuli enhances categorization learning

    Christian Schmid, Muhammad Haziq ... Santiago Jaramillo

    Mice learn a categorization task faster when exposed to task-relevant stimuli outside training, supporting neural-network models in which unsupervised learning first captures the statistical structure of inputs before classifying them.
56. 1. Neuroscience

    The involvement of the human prefrontal cortex in the emergence of visual awareness

    Zepeng Fang, Yuanyuan Dang ... Mingsha Zhang

    While minimizing the report-related motor confounding, event-related potential (ERP), high-frequency power, and functional connectivity of the local field potential (LFP) activity in human prefrontal cortex were enhanced under the emergence of visual awareness.
57. 1. Evolutionary Biology

    Measures of genetic diversification in somatic tissues at bulk and single-cell resolution

    Marius E Moeller, Nathaniel V Mon Père ... Weini Huang

    Single-cell and bulk sequencing data are combined through theoretical modeling to reveal the number of tissue-specific stem cells, mutation, and proliferation rates under sampling.
58. 1. Genetics and Genomics

    Yeast eIF2A has a minimal role in translation initiation and uORF-mediated translational control in vivo

    Swati Gaikwad, Fardin Ghobakhlou ... Alan G Hinnebusch

    Eliminating eukaryotic initiation factor 2 (eIF2) from wild-type budding yeast had little impact on translation of individual mRNAs, including those with regulatory upstream open-reading frames, even when canonical initiation factor eIF2 activity was impaired.
59. 1. Microbiology and Infectious Disease

    Physiological and metabolic insights into the first cultured anaerobic representative of deep-sea Planctomycetes bacteria

    Rikuan Zheng, Chong Wang ... Chaomin Sun

    A deep-sea Planctomycetes bacterium performs a unique budding mode of division and recruits chronic phages for metabolizing nitrogen through the function of auxiliary metabolic genes.
60. 1. Cell Biology

    Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

    Yin Luo, Huanmeng Hao ... Ding Xu

    By binding to TRAIL and inducing its oligomerization, heparan sulfate plays a role in regulating the pro-apoptotic activity of TRAIL towards several types of tumor cells.
61. 1. Evolutionary Biology
    2. Medicine

    Taste shaped the use of botanical drugs

    Marco Leonti, Joanna Baker ... Julie Hawkins

    Plant drugs used by ancient Graeco-Roman societies have tastes and flavours that predict how they were used therapeutically.
62. 1. Genetics and Genomics

    Functional characterization of the disease-associated CCL2 rs1024611G-rs13900T haplotype: The role of the RNA-binding protein HuR

    Feroz Akhtar, Joselin Hernandez Ruiz ... Srinivas Mummidi
63. 1. Neuroscience

    Designing optimal behavioral experiments using machine learning

    Simon Valentin, Steven Kleinegesse ... Christopher G Lucas

    Recent advances in Bayesian optimal experimental design have made it possible to improve the efficiency and informativeness of experiments using machine learning, and shed new light on considerations that affect machine learning assisted experimental designs and computational models in general.
64. 1. Evolutionary Biology
    2. Genetics and Genomics

    Cis-regulatory modes of Ultrabithorax inactivation in butterfly forewings

    Amruta Tendolkar, Anyi Mazo-Vargas ... Arnaud Martin

    CRISPR knock-outs and functional genomic approaches probe the regulatory mechanism restricting the Hox gene Ubx to butterfly hindwings, explaining color pattern differentiation from forewings.
65. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Tracing the substrate translocation mechanism in P-glycoprotein

    Theresa Gewering, Deepali Waghray ... Qinghai Zhang

    New P-glycoprotein structures reveal a substrate translocation mechanism and pathway across lipid bilayers that challenges ATP-binding cassette transporter norms.
66. 1. Microbiology and Infectious Disease

    Interplay between acetylation and ubiquitination of imitation switch chromatin remodeler Isw1 confers multidrug resistance in Cryptococcus neoformans

    Yang Meng, Yue Ni ... Chen Ding

    Isw1 acts as a master regulator in modulating the expression of drug-resistance genes, and this regulatory mechanism is dependent on the interaction of Isw1 acetylation and ubiquitination.
67. 1. Biochemistry and Chemical Biology
    2. Immunology and Inflammation

    Design principles for inflammasome inhibition by pyrin-only-proteins

    Shuai Wu, Archit Garg ... Jungsan Sohn

    Computational analyses and biochemical measurements redefine the mechanisms by which pyrin-only-proteins specifically target and regulate the assembly of inflammasomes.
68. 1. Structural Biology and Molecular Biophysics

    Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius

    Lavinia Gambelli, Mathew McLaren ... Bertram Daum

    CryoEM reveals the structure of a two-component archaeal S-layer, which sheds new light on archaeal cell biology.
69. 1. Cell Biology

    Phosphate starvation signaling increases mitochondrial membrane potential through respiration-independent mechanisms

    Yeyun Ouyang, Mi-Young Jeong ... Jared Rutter

    SIT4 deletion and phosphate starvation increase mitochondrial membrane potential via electron transport chain dependent and independent manners, and the increased membrane potential induced by phosphate depletion is conserved between yeast, flies, and humans.
70. 1. Cancer Biology

    Ferredoxin 1 is essential for embryonic development and lipid homeostasis

    Shakur Mohibi, Yanhong Zhang ... Xinbin Chen

    The iron-sulfur cluster containing Ferredoxin 1 (FDX1), critical in steroidogenesis and TCA cycle, is required for mammalian embryonic development and maintenance of lipid homeostasis at cellular and organismal levels.
71. 1. Chromosomes and Gene Expression
    2. Immunology and Inflammation

    Regulation of nuclear transcription by mitochondrial RNA in endothelial cells

    Kiran Sriram, Zhijie Qi ... Zhen Bouman Chen

    Mitochondrial RNAs attach to chromatin and regulate nuclear transcription in endothelial stress response.
72. 1. Cell Biology
    2. Computational and Systems Biology

    Reprogramming of cardiac phosphoproteome, proteome, and transcriptome confers resilience to chronic adenylyl cyclase-driven stress

    Jia-Hua Qu, Khalid Chakir ... Edward G Lakatta

    Numerous pathways/function predictions were identified via gene sets, phosphopeptides, and phosphoproteins, which may point to potential novel therapeutic targets to enhance heart adaptivity, maintaining heart performance while avoiding cardiac dysfunction.
73. 1. Physics of Living Systems

    Recursive self-embedded vocal motifs in wild orangutans

    Adriano R Lameira, Madeleine E Hardus ... Marco Gamba

    Wild male orangutans produce long calls composed of two hierarchical layers, wherein isochronous sub-pulses occur within isochronous pulses, indicating that recursive vocal combinatorics are not exclusive to language among hominids.
74. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Double and triple thermodynamic mutant cycles reveal the basis for specific MsbA-lipid interactions

    Jixing Lyu, Tianqi Zhang ... Arthur Laganowsky

    Native mass spectrometry reveals the thermodynamic basis for high-affinity lipid binding to the ABC transporter MsbA.
75. 1. Biochemistry and Chemical Biology

    Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space

    Fabio Lolicato, Julia P Steringer ... Walter Nickel

    Disulfide-bridged fibroblast growth factor 2 (FGF2) dimerization at the inner plasma membrane leaflet produces the building block for higher FGF2 oligomers that drive FGF2 membrane translocation into the extracellular space.
76. 1. Microbiology and Infectious Disease

    Coordinated regulation of gene expression in Plasmodium female gametocytes by two transcription factors

    Yuho Murata, Tsubasa Nishi ... Masao Yuda

    Sequential expression of two transcription factors that bind to five- and ten-base female-specific cis-acting elements, respectively, promotes differentiation of Plasmodium female gametocytes.
77. 1. Ecology
    2. Physics of Living Systems

    Investigating macroecological patterns in coarse-grained microbial communities using the stochastic logistic model of growth

    William R Shoemaker, Jacopo Grilli

    Microbial macroecological patterns under coarse-graining are captured by a null model of ecology, a result which was leveraged to successfully predict the relationship between fine and coarse-grained diversity.
78. 1. Neuroscience
    2. Computational and Systems Biology

    Inter-regional delays fluctuate in the human cerebral cortex

    Joon-Young Moon, Kathrin Müsch ... Christopher J. Honey
79. 1. Computational and Systems Biology

    A mathematical model that predicts human biological age from physiological traits identifies environmental and genetic factors that influence aging

    Sergiy Libert, Alex Chekholko, Cynthia Kenyon
80. 1. Cancer Biology

    Formation of malignant, metastatic small cell lung cancers through overproduction of cMYC protein in TP53 and RB1 depleted pulmonary neuroendocrine cells derived from human embryonic stem cells

    Huanhuan Joyce Chen, Eric E. Gardner ... Harold Varmus
81. 1. Neuroscience

    Implications of variable synaptic weights for rate and temporal coding of cerebellar outputs

    Shuting Wu, Asem Wardak ... Wade G Regehr

    Purkinje cell inputs onto cerebellar nuclei (CbN) neurons have highly variable strengths, which allow them to influence CbN firing in a graded manner, with the largest individual inputs effectively regulating both the rate and spike timing of CbN neuron firing.
82. 1. Cell Biology

    Organelle proteomic profiling reveals lysosomal heterogeneity in association with longevity

    Yong Yu, Shihong M Gao ... Meng C Wang

    First systemic lysosomal proteomes in association with longevity and tissue specificity in C. elegans uncover lysosomal heterogeneity across multiple scales and provide valuable resources for understanding how lysosomes modulate signal transduction, organelle communication, tissue physiology, and organism longevity.
83. 1. Chromosomes and Gene Expression

    Monoallelically expressed noncoding RNAs form nucleolar territories on NOR-containing chromosomes and regulate rRNA expression

    Qinyu Hao, Minxue Liu ... Kannanganattu V Prasanth

    A novel family of nucleolus-enriched ncRNAs forms allele-specific territories on the nucleolar organizing region (NOR)-containing acrocentric chromosomes.
84. 1. Chromosomes and Gene Expression

    Regulation of chromatin architecture by transcription factor binding

    Stephanie Portillo-Ledesma, Suckwoo Chung ... Tamar Schlick

    The binding of transcription factors to mesoscale chromatin fibers leads to microdomains whose features are dependent on the linker DNA length, linker histone density, and tail acetylation levels.
85. 1. Neuroscience

    Acetylcholine modulates the precision of prediction error in the auditory cortex

    David Pérez-González, Ana Belén Lao-Rodríguez ... Manuel S Malmierca

    Acetylcholine plays a multifold role in modulating neuronal mismatch in auditory cortex, affecting the precision of prediction error signaling and gating prediction errors to hierarchically higher processing levels.
86. 1. Genetics and Genomics
    2. Medicine

    Tmem263 deletion disrupts the GH/IGF-1 axis and causes dwarfism and impairs skeletal acquisition

    Dylan C Sarver, Jean Garcia-Diaz ... G William Wong

    Mice lacking Tmem263 are dwarfs, and this phenotype is associated with reduced hepatic growth hormone (GH) receptor expression, a deficit in GH-induced signaling, and low insulin-like growth factor 1 level.
87. 1. Computational and Systems Biology

    Analysis of NIH K99/R00 awards and the career progression of awardees

    Nicole C Woitowich, Sarah R Hengel ... Daniel J Tyrrell

    There is a significant disadvantage to receive a major grant (i.e., R01) for K99/R00 awardees that are women, those at lower funded institutions, and those with lower career mobility.
88. 1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Genetic basis of Arabidopsis thaliana responses to infection by naïve and adapted isolates of turnip mosaic virus

    Anamarija Butkovic, Thomas James Ellis ... Santiago F Elena

    Arabidopsis lines were screened for resistance to TuMV, identifying a region on chromosome 2 linked to necrosis that includes an antiviral gene.
89. 1. Physics of Living Systems

    Free volume theory explains the unusual behavior of viscosity in a non-confluent tissue during morphogenesis

    Rajsekhar Das, Sumit Sinha ... D Thirumalai

    Theoretical explanation and a novel mechanism are given for the observation that viscosity of embryonic non-confluent tissue shows glass-like behavior till a critical cell density and saturates at higher densities.
90. 1. Genetics and Genomics

    Auxin exposure disrupts feeding behavior and fatty acid metabolism in adult Drosophila

    Sophie A Fleck, Puja Biswas ... Lesley N Weaver

    Adult Drosophila exposed to auxin at the recommended concentrations for the AGES transgene expression system display defects in feeding behavior, have altered metabolic transcripts, and have reduced triacylglyceride levels.
91. 1. Cell Biology

    N-cadherin mechanosensing in ovarian follicles controls oocyte maturation and ovulation

    Alaknanda Emery, Orest W. Blaschuk ... Darryl L Russell
92. 1. Developmental Biology

    A wave of minor de novo DNA methylation initiates in mouse 8-cell embryos and co-regulates imprinted X- chromosome inactivation

    Yuan Yue, Wei Fu ... Lei An
93. 1. Medicine
    2. Stem Cells and Regenerative Medicine

    The vascularised chamber device significantly enhances the survival of transplanted liver organoids

    Denis D. Shi, Evelyn Makris ... Kiryu K. Yap
94. 1. Cell Biology

    Progesterone induces meiosis through two obligate co-receptors with PLA2 activity

    Nancy Nader, Lama Assaf ... Khaled Machaca
95. 1. Stem Cells and Regenerative Medicine

    An optogenetic cell therapy to restore control of target muscles in an aggressive mouse model of amyotrophic lateral sclerosis

    J Barney Bryson, Alexandra Kourgiantaki ... Linda Greensmith

    Stem-cell-based neural replacement, in combination with optogenetic stimulation, could represent a translationally viable therapeutic strategy to overcome atrophy and paralysis of targeted muscles in people living with amyotrophic lateral sclerosis.
96. 1. Neuroscience

    What happens to the inhibitory control functions of the right inferior frontal cortex when this area is dominant for language?

    Esteban Villar-Rodríguez, Cristina Cano-Melle ... César Avila

    A complementary relationship exists between the hemispheric lateralization of inhibitory control (typically right) and speech (typically left), as observed in typically and atypically lateralized left-handed individuals.
97. 1. Chromosomes and Gene Expression

    Spatial chromatin accessibility sequencing resolves high-order spatial interactions of epigenomic markers

    Yeming Xie, Fengying Ruan ... Chong Tang

    SCA-seq enables simultaneous detection of spatial interactions, chromatin accessibility, and CpG methylation at single-molecule resolution, advancing multi-omics studies of genome spatial organization.
98. 1. Immunology and Inflammation

    Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens

    Munir Akkaya, Jafar Al Souz ... Billur Akkaya

    Novel antigen-targeting probes, featuring a cysteine-rich tag emitting fluorescence, offer unprecedented insight into antigen-specific immune responses.
99. 1. Neuroscience

    CaBP1 and 2 enable sustained Ca_V1.3 calcium currents and synaptic transmission in inner hair cells

    David Oestreicher, Shashank Chepurwar ... Tina Pangrsic
100. 1. Neuroscience

     The Spatial Frequency Representation Predicts Category Coding in the Inferior Temporal Cortex

     Ramin Toosi, Behnam Karami ... Mohammad-Reza A. Dehaqani
101. 1. Evolutionary Biology

     Evolution of the gene regulatory network of body axis by enhancer hijacking in amphioxus

     Chenggang Shi, Shuang Chen ... Guang Li

     The gene regulatory network of Nodal signaling, underpinning body axes patterning, was evolved specifically in cephalochordate lineage.
102. 1. Immunology and Inflammation

     Catalytic activity and autoprocessing of murine caspase-11 mediate noncanonical inflammasome assembly in response to cytosolic LPS

     Daniel C Akuma, Kimberly A Wodzanowski ... Igor E Brodsky

     Caspase-11 catalytic activity and autoprocessing play key roles upstream rather than downstream of its assembly into higher-order inflammasome complexes in response to cytosolic bacterial lipopolysaccharide.
103. 1. Neuroscience

     Neuroendocrine gene expression coupling of interoceptive bacterial food cues to foraging behavior of C. elegans

     Sonia A Boor, Joshua D Meisel, Dennis H Kim

     Distinct interoceptive and gustatory bacterial food cues converge to control neuroendocrine gene expression in two neurons, which modulates and is correlated with internal states driving feeding versus foraging behavior.
104. 1. Neuroscience

     A generative model of electrophysiological brain responses to stimulation

     Diego Vidaurre

     Genephys is a generative model for dissecting the different aspects that compound our neural responses to perceptual stimulation, identifying which aspects remain stable and which ones vary across experimental repetitions.
105. 1. Neuroscience

     14-3-3 protein augments the protein stability of phosphorylated spastin and promotes the recovery of spinal cord injury through its agonist intervention

     Qiuling Liu, Hua Yang ... Zhisheng Ji

     14-3-3 protein augments the protein stability of phosphorylated spastin and promotes the recovery of spinal cord injury in mice.
106. 1. Biochemistry and Chemical Biology

     Isolating Small Extracellular Vesicles from Small Volumes of Blood Plasma using size exclusion chromatography and density gradient ultracentrifugation: A Comparative Study

     Fang Kong, Megha Upadya ... Ming Dao
107. 1. Developmental Biology

     Spatial transcriptomics in adult Drosophila reveals new cell types in the brain and identifies subcellular mRNA patterns in muscles

     Jasper Janssens, Pierre Mangeol ... Frank Schnorrer
108. 1. Neuroscience

     Interaction of human keratinocytes and nerve fiber terminals at the neuro-cutaneous unit

     Christoph Erbacher, Sebastian Britz ... Nurcan Üçeyler

     Sensory nerve fiber ending ensheathment and connexin 43 contacts by keratinocytes at the neuro-cutaneous unit can be visualized and quantified at super-resolution in human skin.
109. 1. Computational and Systems Biology

     Structural assembly of the bacterial essential interactome

     Jordi Gómez Borrego, Marc Torrent Burgas

     A new study reveals structural details of the bacterial essential interactome, opening new avenues for antibiotic discovery.
110. 1. Cell Biology

     Dual recognition of multiple signals in bacterial outer membrane proteins enhances assembly and maintains membrane integrity

     Edward M Germany, Nakajohn Thewasano ... Takuya Shiota

     Bacterial outer membrane proteins are recognized by BamD at specific signals located in multiple β-strands at the C-terminus of these proteins, and this recognition is important for efficient outer membrane protein assembly and maintaining the integrity of outer membrane.
111. 1. Developmental Biology

     Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling

     Steven A Cincotta, Nainoa Richardson ... Diana J Laird

     Although developing oocytes and sperm of mice both harbor the glucocorticoid receptor, fetal oocytes are resistant to differing levels of gluocorcorticoid stress hormones, while spermatogonia alter RNA splicing.
112. 1. Neuroscience

     Population analyses reveal heterogenous encoding in the medial prefrontal cortex during naturalistic foraging

     Ji Hoon Jeong, June-Seek Choi
113. 1. Neuroscience

     Resource-rational account of sequential effects in human prediction

     Arthur Prat-Carrabin, Florent Meyniel, Rava Azeredo da Silveira

     A proposed model of optimal inference under cognitive costs accounts for human sequential effects, including subtle patterns of attractive and repulsive influence of past observations, in a binary prediction task across a wide range of stimulus conditions.
114. 1. Genetics and Genomics

     Multi-ancestry meta-analysis of host genetic susceptibility to tuberculosis identifies shared genetic architecture

     Haiko Schurz, Vivek Naranbhai ... International Tuberculosis Host Genetics Consortium

     Results from the largest tuberculosis genome-wide association study meta-analysis identified a significant association in the human leukocyte antigen class II region, rs28383206, across multiple populations.
115. 1. Medicine
     2. Neuroscience

     Resting-state alterations in behavioral variant frontotemporal dementia are related to the distribution of monoamine and GABA neurotransmitter systems

     Lisa Hahn, Simon B Eickhoff ... Matthias L Schroeter

     Local brain functional activity reductions in bvFTD follow the distribution of serotonergic, GABAergic, and norepinephrinergic neurotransmitter systems, indicating a selective vulnerability and providing novel insights into the underlying disease mechanisms.
116. 1. Microbiology and Infectious Disease

     Dynamics of macrophage polarization support Salmonella persistence in a whole living organism

     Jade Leiba, Tamara Sipka ... Mai E Nguyen-Chi

     Real-time visualization of Salmonella enterica and polarized macrophage interaction in the living host identifies an anti-inflammatory, pro-regenerative, and motionless macrophage subset as a survival niche during persistent infection.
117. 1. Epidemiology and Global Health

     Design of the HPV-automated visual evaluation (PAVE) study: Validating a novel cervical screening strategy

     Silvia de Sanjosé, Rebecca B Perkins ... Mark Schiffman

     The PAVE strategy aims to provide a cost-effective, high quality ‘Screen-Triage-Treat’ approach for cervical cancer screening to save many lives.
118. 1. Neuroscience

     Toolkits for detailed and high-throughput interrogation of synapses in C. elegans

     Maryam Majeed, Haejun Han ... Hang Lu

     An imaging pipeline together with a collection of transgenic nematode strains provide a strategy to visualize and properly quantify synaptic connectivity in the C. elegans brain.
119. 1. Neuroscience

     EphrinB2 knockdown in cervical spinal cord preserves diaphragm innervation in a mutant SOD1 mouse model of ALS

     Mark W Urban, Brittany A Charsar ... Angelo C Lepore

     In the SOD1^G93A mouse model of amyotrophic lateral sclerosis (ALS), expression of transmembrane signaling molecule ephrinB2 in spinal cord astrocytes contributes to motor neuron damage and loss of diaphragm function.
120. 1. Genetics and Genomics

     Leveraging inter-individual transcriptional correlation structure to infer discrete signaling mechanisms across metabolic tissues

     Mingqi Zhou, Ian Tamburini ... Marcus M Seldin

     A population resource to investigate mechanisms of organ communication.
121. 1. Cell Biology
     2. Physics of Living Systems

     Probe-free optical chromatin deformation and measurement of differential mechanical properties in the nucleus

     Benjamin Seelbinder, Susan Wagner ... Moritz Kreysing

     Localized temperature gradients used as a novel method of active micro-theology that is non-invasive and probe-free, to investigate material responses of cell departments such as the different compartments of a nucleus displaying distinct material properties.
122. 1. Neuroscience

     A preclinical model of THC edibles that produces high-dose cannabimimetic responses

     Anthony English, Fleur Uittenbogaard ... Benjamin Bruce Land

     Δ⁹-Tetrahydrocannabinol (THC)-E-gel enables robust preclinical study of voluntary THC consumption in mice, revealing sex-dependent psychotomimetic effects that are valuable for understanding cannabimimetic responses.
123. 1. Neuroscience

     A visual efference copy-based navigation algorithm in Drosophila for complex visual environments

     Angel Canelo, Yeon Kim ... Anmo J Kim
124. 1. Neuroscience

     Genetic and pharmacologic alterations of claudin9 levels suffice to induce functional and mature inner hair cells

     Yingying Chen, Jeong Han Lee ... Ebenezer N. Yamoah
125. 1. Cell Biology

     Location, location, location: Protein kinase nanoclustering for optimised signalling output

     Rachel S Gormal, Ramon Martinez-Marmol ... Frédéric A Meunier

     This article reviews the latest studies that have used super-resolution microscopy and cluster analysis methodologies to study the mechanism of protein kinase signalling hubs and their organisation at nanoscale.
126. 1. Microbiology and Infectious Disease
     2. Structural Biology and Molecular Biophysics

     A Vibrio cholerae viral satellite maximizes its spread and inhibits phage by remodeling hijacked phage coat proteins into small capsids

     Caroline M Boyd, Sundharraman Subramanian ... Kimberley D Seed

     A phage parasite encodes an external scaffolding protein to pirate and rearrange phage-encoded coat proteins to more efficiently transfer the phage parasite genome to new hosts and limit phage production.
127. 1. Computational and Systems Biology

     Long-read single-cell sequencing reveals expressions of hypermutation clusters of isoforms in human liver cancer cells

     Silvia Liu, Yan-Ping Yu ... Jian-Hua Luo

     A synthetic long-read single-cell sequencing reveals critical mutation isoform expression patterns in human liver cancer cells.
128. 1. Biochemistry and Chemical Biology
     2. Cell Biology

     Comparative analysis of two Caenorhabditis elegans kinesins KLP-6 and UNC-104 reveals a common and distinct activation mechanism in kinesin-3

     Tomoki Kita, Kyoko Chiba ... Shinsuke Niwa

     Comparative biochemical analysis of a nematode-specific kinesin-3 shed light on the mechanism of kinesin-3 dimerization and activation.
129. 1. Neuroscience

     Visual experience shapes functional connectivity between occipital and non-visual networks

     Mengyu Tian, Xiang Xiao ... Marina Bedny
130. 1. Genetics and Genomics
     2. Evolutionary Biology

     The Genomic Legacy of Human Management and sex-biased Aurochs hybridization in Iberian Cattle

     Torsten Günther, Jacob Chisausky ... Cristina Valdiosera
131. 1. Neuroscience

     Visual routines for detecting causal interactions are tuned to motion direction

     Sven Ohl, Martin Rolfs
132. 1. Developmental Biology

     Gcn5 – mTORC1 – TFEB signalling axis mediated control of autophagy regulates Drosophila blood cell homeostasis

     AR Arjun, Suraj Math ... Rohan Jayant Khadilkar
133. 1. Neuroscience

     Progressively shifting patterns of co-modulation among premotor cortex neurons carry dynamically similar signals during action execution and observation

     Zhonghao Zhao, Marc H. Schieber
134. 1. Neuroscience
     2. Physics of Living Systems

     Sub-surface deformation of individual fingerprint ridges during tactile interactions

     Giulia Corniani, Zing S Lee ... Hannes P Saal
135. 1. Neuroscience

     Early roots of information-seeking: Infants predict and generalize the value of information

     Tommaso Ghilardi, Francesco Poli ... Sabine Hunnius
136. 1. Microbiology and Infectious Disease

     Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency viruses

     Cody J. Warren, Arturo Barbachano-Guerrero ... Sara L. Sawyer
137. 1. Chromosomes and Gene Expression

     A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination

     Salman Shehzada, Tomoko Noto ... Kazufumi Mochizuki

     The identification of a SUMO E3 ligase engaged in lncRNA transcription, subsequently leading to target-directed small RNA degradation, reveals an unexplored layer of regulatory mechanisms within small RNA-directed chromatin regulation.
138. 1. Computational and Systems Biology

     Affected cell types for hundreds of Mendelian diseases revealed by analysis of human and mouse single-cell data

     Idan Hekselman, Assaf Vital ... Esti Yeger-Lotem

     The PrEDiCT scheme infers disease-affected cell types from the expression of disease-associated genes in single-cell expression atlases, thereby providing cellular context and enhancing mechanistic understanding for 328 Mendelian diseases.
139. 1. Neuroscience

     The Na⁺ leak channel NALCN controls spontaneous activity and mediates synaptic modulation by α2-adrenergic receptors in auditory neurons

     Tenzin Ngodup, Tomohiko Irie ... Laurence O Trussell

     Noradrenergic receptors can control the spontaneous electrical firing of neurons by inhibiting a Na⁺ channel known as NALCN using a pathway shared by metabotropic GABA receptors.
140. 1. Neuroscience

     A comprehensive neuroanatomical survey of the Drosophila Lobula Plate Tangential Neurons with predictions for their optic flow sensitivity

     Arthur Zhao, Aljoscha Nern ... Michael B. Reiser
141. 1. Biochemistry and Chemical Biology

     Acyl-CoA thioesterase-2 facilitates β-oxidation in glycolytic skeletal muscle in a lipid supply dependent manner

     Carmen Bekeova, Ji In Han ... Erin L. Seifert
142. 1. Neuroscience
     2. Evolutionary Biology

     A single-cell transcriptomic atlas reveals resident dendritic-like cells in the zebrafish brain parenchyma

     Mireia Rovira, Giuliano Ferrero ... Valérie Wittamer
143. 1. Biochemistry and Chemical Biology
     2. Genetics and Genomics

     Inhibition of mitochondrial protein import and proteostasis by a pro-apoptotic lipid

     Josep Fita-Torró, José Luis Garrido-Huarte ... Markus Proft
144. 1. Cell Biology

     ARHGAP18-ezrin functions as an autoregulatory module for RhoA in the assembly of distinct actin-based structures

     Andrew T Lombardo, Cameron AR Mitchell ... Anthony Bretscher

     ARHGAP18 specializes in controlling cell shape at the topmost layer of the cell membrane by regulating the interaction between the ERM protein family, RhoA signaling, and the actin cytoskeleton.
145. 1. Developmental Biology

     Intrinsic and extrinsic cues time somite progenitor contribution to the vertebrate primary body axis

     Lara Busby, Guillermo Serrano Nájera, Benjamin John Steventon

     Cells know when to contribute to the maturing embryonic body axis by adjusting an internal timer to the conditions around them.
146. 1. Neuroscience

     A translational MRI approach to validate acute axonal damage detection as an early event in multiple sclerosis

     Antonio Cerdán Cerdá, Nicola Toschi ... Silvia De Santis

     MRI is sensitive to the increase in axonal caliber due to pathology and was used to detect widespread increase in the average axonal caliber in multiple sclerosis brains with short disease duration only.
147. 1. Neuroscience

     MTL neurons phase-lock to human hippocampal theta

     Daniel R Schonhaut, Aditya M Rao ... Michael J Kahana

     Neural spiking throughout the MTL is synchronous with hippocampal theta phase during spatial memory and navigation experiments in humans, even after controlling for phase-coupling to local theta oscillations.
148. 1. Ecology
     2. Evolutionary Biology

     Fin whale song evolution in the North Atlantic

     Miriam Romagosa, Sharon Nieukirk ... Mónica A Silva

     The rapid and gradual fin whale songs changes adopted by individuals of a certain area provide evidence of vocal learning in this species, elucidate patterns of song evolution and hints on the limits of song variation.
149. 1. Ecology

     Role of immigrant males and muzzle contacts in the uptake of a novel food by wild vervet monkeys

     Pooja Dongre, Gaëlle Lanté ... Erica van de Waal

     New immigrants triggered fast uptake of a novel food in groupmates, who obtained information about it through muzzle contacts, opening up new questions about the role of dispersers in information flow around a population.
150. 1. Neuroscience

     Unraveling the developmental dynamic of visual exploration of social interactions in autism

     Nada Kojovic, Sezen Cekic ... Marie Schaer

     Children with ASD showed a significant divergence in gaze patterns compared to typically developing children, intensifying over early childhood, with implications for developmental and adaptive functioning.
151. 1. Immunology and Inflammation

     Ym1 protein crystals promote type 2 immunity

     Ines Heyndrickx, Kim Deswarte ... Bart N Lambrecht

     Protein crystallization is a rare event yet Ym1 crystals made from a chitinase-like protein are abundantly found in eosinophilic airway inflammation, where they promote type 2 immunity.
152. 1. Neuroscience

     Experience shapes chandelier cell function and structure in the visual cortex

     Koen Seignette, Nora Jamann ... Christiaan N Levelt

     Chandelier cells in primary visual cortex show experience-dependent plasticity, respond strongly to behaviorally relevant stimuli and only weakly inhibit pyramidal cells.
153. 1. Immunology and Inflammation
     2. Microbiology and Infectious Disease

     The infection-tolerant white-footed deermouse tempers interferon responses to endotoxin in comparison to the mouse and rat

     Ana Milovic, Jonathan V Duong, Alan G Barbour

     Comparing the white-footed deermouse with mice and rats in an inflammation model reveals a means for the observed infection tolerance in this key animal reservoir for several human diseases.
154. 1. Biochemistry and Chemical Biology
     2. Stem Cells and Regenerative Medicine

     BRAIDing receptors for cell-specific targeting

     Hui Chen, Sung-Jin Lee ... Yang Li

     A novel strategy to activate the WNT pathway in a cell type selective manner by linking designer ligands with target cell specific bridging receptors.
155. 1. Developmental Biology

     Notch signaling and Bsh homeodomain activity are integrated to diversify Drosophila lamina neuron types

     Chundi Xu, Tyler B Ramos ... Chris Q Doe

     Notch signaling specifies binary neuronal fates through its integration with primary homeodomain activity.
156. 1. Neuroscience

     Balance of activity during a critical period tunes a developing network

     Iain Hunter, Bramwell Coulson ... Richard A Baines

     Neurodevelopmental critical periods act to integrate activity to encode homeostatic set points that remain fixed thereafter.
157. 1. Neuroscience

     Pain persists in mice lacking both Substance P and CGRPα signaling

     Donald Iain MacDonald, Monessha Jayabalan ... Alexander Chesler
158. 1. Structural Biology and Molecular Biophysics

     Interaction of GAT1 with sodium ions: from efficient recruitment to stabilisation of substrate and conformation

     Erika Lazzarin, Ralph Gradisch ... Thomas Stockner
159. 1. Cell Biology

     Cohesin still drives homologous recombination repair of DNA double-strand breaks in late mitosis

     Jessel Ayra-Plasencia, Lorraine Symington, Félix Machín
160. 1. Biochemistry and Chemical Biology

     Redox Regulation of Brain Selective Kinases BRSK1/2: Implications for Dynamic Control of the Eukaryotic AMPK family through Cys-based mechanisms

     George N. Bendzunas, Dominic P Byrne ... Natarajan Kannan
161. 1. Physics of Living Systems

     Hydrodynamics and multiscale order in confluent epithelia

     Josep-Maria Armengol-Collado, Livio Nicola Carenza, Luca Giomi

     Confluent cellular layers display the remarkable ability of supportingmultiscale orientational order, that is the existence of different typeof liquid crystal order at different length scales.
162. 1. Evolutionary Biology
     2. Genetics and Genomics

     Separating phases of allopolyploid evolution with resynthesized and natural Capsella bursa-pastoris

     Tianlin Duan, Adrien Sicard ... Martin Lascoux

     A comparison between artificially created allopolyploids and natural ones reveals that the latter underwent significant evolutionary steps after their formation.
163. 1. Biochemistry and Chemical Biology
     2. Ecology

     Molecular mechanisms of microbiome modulation by the eukaryotic secondary metabolite azelaic acid

     Ahmed A Shibl, Michael A Ochsenkühn ... Shady A Amin

     Characterization of the molecular mechanisms that enable azelaic acid, a secondary metabolite produced by photosynthetic organisms, to promote or inhibit different bacterial populations.
164. 1. Cell Biology
     2. Immunology and Inflammation

     DNA damage signaling in Drosophila macrophages modulates systemic cytokine levels in response to oxidative stress

     Fabian Hersperger, Tim Meyring ... Katrin Kierdorf

     Immune activation in Drosophila macrophages during oxidative stress is regulated by DNA damage signaling, which controls proinflammatory cytokine release and the susceptibility of the fly to oxidative stress.
165. 1. Structural Biology and Molecular Biophysics

     Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states

     David Yin-wei Lin, Lauren E Kueffer ... Amy H Andreotti

     Multiple dynamic protein interactions converge to regulate the catalytic activity of the B cell kinase, BTK.
166. 1. Chromosomes and Gene Expression

     Gle1 is required for tRNA to stimulate Dbp5 ATPase activity in vitro and promote Dbp5-mediated tRNA export in vivo in Saccharomyces cerevisiae

     Arvind Arul Nambi Rajan, Ryuta Asada, Ben Montpetit

     In vivo and in vitro characterization demonstrates a direct interaction of Dbp5 with tRNA that requires Gle1 to spatially activate the Dbp5 ATPase cycle for tRNA export in Saccharomyces cerevisiae.
167. 1. Computational and Systems Biology
     2. Cancer Biology

     Computational Engineering of a Therapeutic Antibody to Inhibit Multiple Mutants of HER2 Without Compromising Inhibition of the Canonical HER2

     Sapir Peled, Julia Guez-Haddad ... Yanay Ofran
168. 1. Neuroscience
     2. Physics of Living Systems

     Criticality supports cross-frequency cortical-thalamic information transfer during conscious states

     Daniel Toker, Eli Müller ... Martin M Monti

     Cross-frequency communication between the cortex and thalamus is linked to consciousness and changes in unconscious and psychedelic states, possibly due to shifts in brain dynamics between stability and chaos.
169. 1. Structural Biology and Molecular Biophysics

     Structural and mechanistic insights into ribosomal ITS2 RNA processing by nuclease-kinase machinery

     Jiyun Chen, Hong Chen ... Liang Liu

     The structures of full-length Las1-Grc3 complexes provide crucial insights into the activation mechanism of HEPN nuclease and the pathway of pre-rRNA processing in ribosome biosynthesis.
170. 1. Neuroscience

     Inhibitory CCK+ basket synapse defects in mouse models of dystroglycanopathy

     Jennifer N Jahncke, Daniel S Miller ... Kevin M Wright

     Loss of functional dystroglycan disrupts the formation and function of CCK⁺/CB₁R⁺ inhibitory synapses in hippocampal CA1, resulting in reduced seizure thresholds in mouse models of dystroglycanopathy.
171. 1. Genetics and Genomics
     2. Medicine

     Proteomic analysis shows decreased type I fibers and ectopic fat accumulation in skeletal muscle from women with PCOS

     Elisabet Stener-Victorin, Gustaw Eriksson ... Anna Benrick

     Elevated levels of ectopic fat and a decreased number of oxidative insulin-sensitive type I muscle fibers in skeletal muscle could lead to insulin resistance in women with polycystic ovary syndrome.
172. 1. Neuroscience

     Acute stress reduces effortful prosocial behaviour

     Paul AG Forbes, Gökhan Aydogan ... Claus Lamm

     Participants under acute stress were less willing to exert a relatively low level of physical effort for actions that benefit another person compared to actions that benefit themselves.
173. 1. Neuroscience

     A maximum of two readily releasable vesicles per docking site at a cerebellar single active zone synapse

     Melissa Silva, Van Tran, Alain Marty

     Counting the number of synaptic vesicles released in simple synapses under high release probability conditions revealed a maximum readily releasable pool size of two vesicles per docking site.
174. 1. Biochemistry and Chemical Biology
     2. Cell Biology

     Interface-acting nucleotide controls polymerization dynamics at microtubule plus- and minus-ends

     Lauren A McCormick, Joseph M Cleary ... Luke M Rice

     Comparative measurements and simulations of microtubule plus and minus end growth with mixed nucleotides demonstrate that nucleotide acts across a tubulin:tubulin interface to influence microtubule stability and dynamics.
175. 1. Developmental Biology
     2. Stem Cells and Regenerative Medicine

     Regulation of multiple signaling pathways promotes the consistent expansion of human pancreatic progenitors in defined conditions

     Luka Jarc, Manuj Bandral ... Anthony Gavalas

     The signaling requirements to decouple proliferation of pancreatic progenitors from differentiation were elucidated and employed for the reproducible expansion, under GMP-compliant conditions, of pancreatic progenitors derived from different human pluripotent stem cell lines.
176. 1. Developmental Biology

     Homeodomain proteins hierarchically specify neuronal diversity and synaptic connectivity

     Chundi Xu, Tyler B Ramos ... Chris Q Doe

     The homeodomain protein Bsh increases lamina neuron types and visual function.
177. 1. Neuroscience

     Cell type-specific connectome predicts distributed working memory activity in the mouse brain

     Xingyu Ding, Sean Froudist-Walsh ... Xiao-Jing Wang

     Large-scale modeling of the mouse brain revealed that working memory is modular and determined by long-range cell type-specific connections, thereby providing a framework to interpret brain-wide recordings during cognition.
178. 1. Neuroscience

     Altered reactivity to threatening stimuli in Drosophila models of Parkinson’s disease, revealed by a trial-based assay

     Márton Kajtor, Viktor A. Billes ... Balázs Hangya
179. 1. Developmental Biology
     2. Neuroscience

     Commissureless acts as a substrate adapter in a conserved Nedd4 E3 ubiquitin ligase pathway to promote axon growth across the midline

     Kelly G Sullivan, Greg J Bashaw
180. 1. Biochemistry and Chemical Biology
     2. Neuroscience

     Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques

     Charlotte Rimbault, Christelle Breillat ... Matthieu Sainlos

     Engineering new intrabodies allows to develop selective and non-interfering tools for the visualization of endogenous PSD-95 with advanced imaging techniques.
181. 1. Immunology and Inflammation
     2. Structural Biology and Molecular Biophysics

     Asymmetric framework motion of TCRαβ controls load-dependent peptide discrimination

     Ana C Chang-Gonzalez, Robert J Mallis ... Wonmuk Hwang

     All-atom molecular dynamics simulations of an αβ T-cell receptor complexed with the major histocompatibility complex molecule presenting wild-type or mutant antigenic peptides reveal how it uses conserved framework motion to discriminate antigens by leveraging physiological force applied during immune surveillance.
182. 1. Stem Cells and Regenerative Medicine

     Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells

     Matias I Autio, Efthymios Motakis ... Roger SY Foo

     Depiction of novel human genomic safe harbour loci that have been validated in vitro for safety and stable expression in human embryonic stem cells and cell types from all the three germ lineages.
183. 1. Developmental Biology
     2. Neuroscience

     RNA fusion in human retinal development

     Wen Wang, Xiao Zhang ... Zi-Bing Jin

     Chimeric RNAs are widely distributed spatiotemporally during human retinal development and have important regulatory functions, such as silencing of CTNNBIP1-CLSTN1 biasing the progenitor cells toward the RPE cell fate at the expense of neural retinal cell fates.