May 2017

Research articles

    1. Cell Biology

    IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium

    Jenna L Wingfield et al
    Multimegadalton intraflagellar transport (IFT) trains assemble by sequential recruitment of IFT subcomplexes from the cell body to the ciliary basal bodies and tubulin, the main IFT cargo, is loaded briefly before trains depart.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cancer Biology

    Synergistic interactions with PI3K inhibition that induce apoptosis

    Yaara Zwang et al
    A geneome-scale shRNA screen identifies five genes whose suppression promotes cell death upon PI3K inhibition both in vitro and in vivo, thus suggesting potential combination therapies involving PI3K inhibition.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Developmental Biology and Stem Cells

    Oxidative stress induces stem cell proliferation via TRPA1/RyR-mediated Ca2+ signaling in the Drosophila midgut

    Chiwei Xu et al
    In response to tissue damage, reactive oxygen species can be sensed by cation channels TRPA1/RyR to cause increases of cytosolic Ca2+ in intestinal stem cells, activating Ras/MAPK activity and stimulating stem cell proliferation in Drosophila.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Structural basis for anion conduction in the calcium-activated chloride channel TMEM16A

    Cristina Paulino et al
    Single-particle cryo-EM and electrophysiology studies of the chloride channel TMEM16A reveals the structural basis for anion conduction and uncover its relationship to lipid scramblases of the same family.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Learning shapes the aversion and reward responses of lateral habenula neurons

    Daqing Wang et al
    Neurons in the lateral habenula are activated by pain, bitterness and social defeat, and their responses are dynamically shaped by learning, suggesting a role in experience-dependent selection of behavioral actions to stressors.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Biophysics and Structural Biology

    The force-sensing peptide VemP employs extreme compaction and secondary structure formation to induce ribosomal stalling

    Ting Su et al
    The structure of the VemP-stalled ribosome reveals a helix-double turn-helix conformation of the nascent chain within the ribosomal tunnel, illustrating how secondary structure formation directly at the peptidyltransferase center of the ribosome can induce translational arrest.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Developmental Biology and Stem Cells

    Control of PNG kinase, a key regulator of mRNA translation, is coupled to meiosis completion at egg activation

    Masatoshi Hara et al
    The kinase that controls maternal mRNA translation is regulated by phosphorylation of its activating subunit to restrict kinase activity to the developmental window between meiosis completion and early embryogenesis.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Threat of shock increases excitability and connectivity of the intraparietal sulcus

    Nicholas L Balderston et al
    Multimodal neuroimaging was used to study the effect of threat on spontaneous brain activity and functional connectivity, and demonstrated that threat increases both activity and connectivity of the intraparietal sulcus, a key node in the frontoparietal attention network.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Activation of the same mGluR5 receptors in the amygdala causes divergent effects on specific versus indiscriminate fear

    Mohammed Mostafizur Rahman et al
    Activation of the same glutamate receptor in the lateral amygdala gives rise to distinct effects on specific versus indiscriminate fear by modulating intrinsic excitability and synaptic plasticity.
    Short Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genes and Chromosomes

    Cooperation between a hierarchical set of recruitment sites targets the X chromosome for dosage compensation

    Sarah Elizabeth Albritton et al
    Gene regulatory elements can target a chromatin regulatory complex to a single chromosome in the genome through hierarchical specification and long distance cooperation.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells
    2. Human Biology and Medicine

    Erythropoietin signaling regulates heme biosynthesis

    Jacky Chung et al
    EPO/JAK2/PKA signaling cascade via AKAP10 relocalization to the outer mitochondrial membrane results in the phosphorylation of the terminal heme synthesis enzyme ferrochelatase, which contributes to heme production in red cells.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Genes and Chromosomes

    Methylated cis-regulatory elements mediate KLF4-dependent gene transactivation and cell migration

    Jun Wan et al
    A new paradigm of DNA methylation mediated gene activation and chromatin remodeling provides a general framework to dissect the biological functions of DNA methylation readers and effectors.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine
    2. Neuroscience

    Rai1 frees mice from the repression of active wake behaviors by light

    Shanaz Diessler et al
    Halving dosage of the Smith-Magenis syndrome responsible gene Rai1 in the mouse greatly amplifies the direct, suppressing effects of light on active-wake behavior through increased activation of the ventral-subparaventricular zone.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Asymmetric recognition of HIV-1 Envelope trimer by V1V2 loop-targeting antibodies

    Haoqing Wang et al
    Single particle cryoEM structure of antibody complex with HIV-1 envelope trimer reveals new mode of asymmetric recognition of trimer apex.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Microbiology and Infectious Disease

    Efficacy of β-lactam/β-lactamase inhibitor combination is linked to WhiB4-mediated changes in redox physiology of Mycobacterium tuberculosis

    Saurabh Mishra et al
    Intrinsic tolerance of Mycobacterium tuberculosis toward the world's most successful antibacterials, β-lactams, is dependent on cytoplasmic redox potential and an intracellular redox-sensor WhiB4.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology

    The step-wise pathway of septin hetero-octamer assembly in budding yeast

    Andrew Weems, Michael McMurray
    In budding yeast cells, slow monomeric septin GTPase activity and the cytosolic GTP:GDP ratio dictate the relative incorporation of alternative subunits during septin hetero-oligomer assembly, which proceeds by discrete, ordered steps dependent on allostery between interaction interfaces.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Chemotactic network responses to live bacteria show independence of phagocytosis from chemoreceptor sensing

    Netra Pal Meena, Alan R Kimmel
    Eukaryotic chemotaxis to live bacteria was quantified at a high throughput level, for the first time, and mechanistically examined for the interrelationship between chemotaxis and phagocytosis.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Biophysics and Structural Biology

    Pausing guides RNA folding to populate transiently stable RNA structures for riboswitch-based transcription regulation

    Hannah Steinert et al
    The discontinuous speed of transcription enables riboswitch molecules to adopt meta-stable structures in response to the presence of their cognate ligand, thereby gene-regulation by means of structure induced transcription termination can occur.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Distinct responses of Purkinje neurons and roles of simple spikes during associative motor learning in larval zebrafish

    Thomas C Harmon et al
    Discrete classes of cerebellar Purkinje neurons show distinct changes in synaptic and spiking activity during motor learning, with simple spikes playing a shifting role during acquisition, expression, and maintenance of learned responses.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells
    2. Neuroscience

    Pioneer neurog1 expressing cells ingress into the otic epithelium and instruct neuronal specification

    Esteban Hoijman et al
    Quantitative 4D image analysis identifies a group of cells which enter into the otic primordium during morphogenesis and instructs neuronal specification.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Rapid short-term reorganization in the language network

    Gesa Hartwigsen et al
    The beneficial contribution of a language network for a specific function depends on the level of functional disruption and may reflect the differential compensatory potential of distinct language networks.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genes and Chromosomes
    2. Genomics and Evolutionary Biology

    The dynamic three-dimensional organization of the diploid yeast genome

    Seungsoo Kim et al
    Yeast homologous chromosomes exhibit condition-specific mitotic pairing at both global and local scales.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complex

    Guijun Shang et al
    PlexinD1 binding releases GIPC autoinhibition, leading to formation of high-order oligomers of the GIPC/myosin VI complex.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Developmental Biology and Stem Cells

    Patterned cortical tension mediated by N-cadherin controls cell geometric order in the Drosophila eye

    Eunice HoYee Chan et al
    N-cadherin at heterotypic contacts controls the level and asymmetric localisation of Myosin-II motor, thereby influencing cell shapes and cell packing.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells
    2. Genes and Chromosomes

    Enforcement of developmental lineage specificity by transcription factor Oct1

    Zuolian Shen et al
    Oct1 replaces Oct4 at critical targets shortly after ESC differentiation to promote expression of lineage-specific genes and repress lineage-inappropriate genes.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Computational and Systems Biology
    2. Genes and Chromosomes

    RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases

    Sofia Battaglia et al
    In vivo and in vitro elongation factor-RNA interaction data provide a missing link in understanding how processive elongation complexes are formed on active genes and disassembled at the end of genes.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Identification of a novel spinal nociceptive-motor gate control for Aδ pain stimuli in rats

    Dvir Blivis et al
    A novel mechanism for gating nociceptive sensory-motor behavior is identified in freely behaving rats using high-speed videography that is controlled by posture and modulated by opioid and non-opioid receptor-dependent processes.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Calcium dynamics regulating the timing of decision-making in C. elegans

    Yuki Tanimoto et al
    A series of quantitative behavioural and opto-physiological analyses using a novel robot microscope system reveals that C. elegans computes the time-differential and time-integral of sensory information for decision-making during olfactory navigation.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt)

    Timothy Erickson et al
    A zebrafish model for a particular form of human deafness (DFNB63) changes our view of this disease by revealing a defect in the localization of Transmembrane channel-like proteins that are essential for mechanotransduction in sensory cells.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine
    2. Neuroscience

    mTORC1 in AGRP neurons integrates exteroceptive and interoceptive food-related cues in the modulation of adaptive energy expenditure in mice

    Luke K Burke et al
    AGRP neurons integrate environmental food-related cues with internal metabolic signals to modulate interscapular brown adipose tissue thermogenesis and energy expenditure, at least in part, via mTORC1 signalling.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Neuroscience

    Optocontrol of glutamate receptor activity by single side-chain photoisomerization

    Viktoria Klippenstein et al
    Introduction of single photoswitchable unnatural amino acid into a neuronal receptor provides reversible, rapid and robust control of its activity by light, representing an important contribution to the fast expanding field of optopharmacology.
    Tools and Resources
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Genes and Chromosomes

    Srs2 promotes synthesis-dependent strand annealing by disrupting DNA polymerase δ-extending D-loops

    Jie Liu et al
    The Srs2 helicase functions in Synthesis-Dependent Strand Annealing to avoid crossover formation during homologous recombination by disrupting D-loops that are extended by DNA polymerase delta in an ATP-dependent and direction-specific manner.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Angular velocity integration in a fly heading circuit

    Daniel Turner-Evans et al
    A distinctive recurrent network motif in the Drosophila central brain enables neurons that encode angular velocity to shift population activity in compass neurons, thereby updating their heading representation whenever the fly turns.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Wiring variations that enable and constrain neural computation in a sensory microcircuit

    William F Tobin et al
    Systematic and coordinated variations in morphology and connectivity can structurally tune a microcircuit's computation but non-systematic variably also exists, imparting "connection noise" that potentially limits processing performance.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    A genetic basis for molecular asymmetry at vertebrate electrical synapses

    Adam C Miller et al
    Electrical synapses are neuronal, gap-junction-based connections found throughout the nervous system that, in the escape circuit of zebrafish, are composed of molecularly distinct pre- and postsynaptic junction forming proteins that are required for behavioral performance.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Immunology

    The structure of a LAIR1-containing human antibody reveals a novel mechanism of antigen recognition

    Fu-Lien Hsieh, Matthew K Higgins
    The structure of a human antibody in which the variable loops position a human protein LAIR1 for antigen recognition, reveals a novel and indirect mode of antibody function.
    Short Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Microbiology and Infectious Disease

    A widespread family of serine/threonine protein phosphatases shares a common regulatory switch with proteasomal proteases

    Niels Bradshaw et al
    Structures of active and inactive conformations of a PP2C family phosphatase reveal a conserved switch that controls enzymatic activity and point to an unexpected relationship between phosphatases and proteasomal proteases.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    The N-terminus of the prion protein is a toxic effector regulated by the C-terminus

    Bei Wu et al
    A novel auto-inhibitory mechanism regulates the functional activity of the cellular prion protein, PrPC, providing for the first time a coherent molecular model for both its pathological and physiological effects.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Chronic pain induces generalized enhancement of aversion

    Qiaosheng Zhang et al
    Chronic pain distorts intensity coding in the anterior cingulate cortex to give rise to generalized anatomically nonspecific enhancement in pain aversion.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Immunology

    Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation

    Daniëlle Rianne José Verboogen et al
    A novel microscopy-based assay shows that dendritic cells encountering pathogenic stimuli form increased complexes of specific SNARE proteins, driving release of large amounts of inflammatory cytokines.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry

    Chemical structure-guided design of dynapyrazoles, cell-permeable dynein inhibitors with a unique mode of action

    Jonathan B Steinman et al
    Analyses of the chemical structure of ciliobrevins led to dynein inhibitors with improved properties and higher potency.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Functional dissociation of stimulus intensity encoding and predictive coding of pain in the insula

    Stephan Geuter et al
    Pain processing in certain brain regions implements predictive coding principles, offering a novel perspective on the neural basis of pain perception.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Plant Biology

    ER assembly of SNARE complexes mediating formation of partitioning membrane in Arabidopsis cytokinesis

    Matthias Karnahl et al
    SNARE proteins are delivered as complexes already from the endoplasmic reticulum along the secretory pathway to the cell division plane to mediate the formation of the partitioning membrane by vesicle fusion.
    Short Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts

    Jorge M Santos et al
    A small surface protein regulates gliding motility and host cell invasion by the sporozoite stage of the malaria parasite.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Immunology

    Functionally diverse human T cells recognize non-microbial antigens presented by MR1

    Marco Lepore et al
    MR1T cells are human polyclonal T cells endowed with diverse effector functions in response to endogenous antigens presented by MHC-class 1-related molecule, MR1.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genomics and Evolutionary Biology

    On the mechanistic nature of epistasis in a canonical cis-regulatory element

    Mato Lagator et al
    Thermodynamic framework accurately predicts the sign of epistasis and its environment dependence for a canonical cis-regulatory element.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    A positive feedback loop linking enhanced mGluR function and basal calcium in spinocerebellar ataxia type 2

    Pratap Meera et al
    A damaging form of positive feedback linking elevated calcium levels and metabotropic glutamate receptor function in Purkinje neurons plays a critical role in the pathology in spinocerebellar ataxias.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Microbiology and Infectious Disease

    A unifying mechanism for the biogenesis of membrane proteins co-operatively integrated by the Sec and Tat pathways

    Fiona J Tooke et al
    Bioinformatics and experimental approaches identify families of membrane proteins requiring the co-ordinated action of the Sec pathway and Tat pathways for their integration and define features of the polypeptides that mediate interaction with these pathways.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells

    Dissection of zebrafish shha function using site-specific targeting with a Cre-dependent genetic switch

    Kotaro Sugimoto et al
    A versatile invertible gene trap vector is generated and inserted into a defined genomic locus in zebrafish via homologous recombination, demonstrating an efficient genetic approach to performing conditional knockout analysis.
    Tools and Resources
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Computational and Systems Biology

    Distributing tasks via multiple input pathways increases cellular survival in stress

    Alejandro A Granados et al
    Single-cell experiments and mathematical modelling show that cellular signalling networks are vulnerable to trade-offs in speed versus accuracy, but that these vulnerabilities can be overcome by distributing the two tasks to different, although interacting, subnetworks.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology

    A bioactive peptide amidating enzyme is required for ciliogenesis

    Dhivya Kumar et al
    Analysis of Chlamydomonas, planaria and mice reveals a novel and unanticipated role for a peptide amidating enzyme in primary and motile ciliary assembly through effects on post-Golgi trafficking.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Developmental Biology and Stem Cells

    Macrophages are necessary for epimorphic regeneration in African spiny mice

    Jennifer Simkin et al
    Inflammatory cells differentially regulate a scarring versus a regenerating response to injury in mammals.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    An inhibitory gate for state transition in cortex

    Stefano Zucca et al
    Two major subtypes of cortical interneurons, the PV and the SST positive cells, causally contribute to the regulation of large-scale state transitions in the cortex.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Identification of octopaminergic neurons that modulate sleep suppression by male sex drive

    Daniel R Machado et al
    The balance between sleep and sex drives determines whether male flies sleep or court, and a subset of octopaminergic neurons interact with the Fruitless-expressing courtship circuit to suppress sleep for sustained courtship.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Voltage-gated Na+ currents in human dorsal root ganglion neurons

    Xiulin Zhang et al
    Human sensory neurons may not only bridge a critical gap between drug discovery and clinical trials, but force a re-evaluation of basic assumptions about the mechanisms controlling primary afferent excitability.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Microbiology and Infectious Disease

    Plasmodium P36 determines host cell receptor usage during sporozoite invasion

    Giulia Manzoni et al
    Host CD81 and Scavenger Receptor BI operate independently to mediate invasion of hepatocytes by different species of Plasmodium sporozoites, which use the parasite protein P36 as a key determinant of the entry route.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Human Biology and Medicine
    2. Neuroscience

    Humans treat unreliable filled-in percepts as more real than veridical ones

    Benedikt V Ehinger et al
    In a forced decision between two identical percepts, subjects preferentially rely on inferred internally generated over veridically seen percepts.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Catecholaminergic challenge uncovers distinct Pavlovian and instrumental mechanisms of motivated (in)action

    Jennifer C Swart et al
    Motivational coupling of action to reward and inhibition to punishment is subserved by dissociable learning and choice processes, and is modulated by dopamine/noradrenaline transporter blockade.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    The murine catecholamine methyltransferase mTOMT is essential for mechanotransduction by cochlear hair cells

    Christopher L Cunningham et al
    Murine TOMT is essential for transport of mechanotransduction components to stereocilia in cochlear hair cells.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Biophysics and Structural Biology

    Helical jackknives control the gates of the double-pore K+ uptake system KtrAB

    Marina Diskowski et al
    The K(+) uptake system KtrAB is controlled by an allosteric mechanism entirely new for membrane channels, which operates the channel gate over a 35 Å distance.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology

    The Sec61 translocon limits IRE1α signaling during the unfolded protein response

    Arunkumar Sundaram et al
    Building on previous work (Plumb et al., 2015), it is shown that the Sec61 translocon controls the oligomerization, activation and inactivation of Ire1α during endoplasmic reticulum stress.
    Research Advance
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Structural basis of cooperativity in kinesin revealed by 3D reconstruction of a two-head-bound state on microtubules

    Daifei Liu et al
    A novel algorithm is used to solve the first 3D reconstruction of a stepping kinesin dimer on microtubules, directly visualizing the conformational effects of inter-head strain and giving novel insights into the motility mechanism.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Genes and Chromosomes

    Mutational phospho-mimicry reveals a regulatory role for the XRCC4 and XLF C-terminal tails in modulating DNA bridging during classical non-homologous end joining

    Davide Normanno et al
    In concert phosphorylation site modification of both XRCC4 and XLF C-terminal disordered tails promotes disassembly of XRCC4/XLF DNA tethers during c-NHEJ.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Neuroscience

    Enhanced FIB-SEM systems for large-volume 3D imaging

    C Shan Xu et al
    Enabling complete neuronal circuit reconstruction and whole-cell imaging of eukaryotes at less than 10 nm isotropic resolution.
    Tools and Resources
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Cell Biology

    Sphingomyelin metabolism controls the shape and function of the Golgi cisternae

    Felix Campelo et al
    Association of curvature generating proteins to the Golgi membranes by sphingomyelin metabolism essentially controls the flatness of a Golgi cisterna that is necessary for efficient sorting and export.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Immunology

    Synthekines are surrogate cytokine and growth factor agonists that compel signaling through non-natural receptor dimers

    Ignacio Moraga et al
    Synthetic cytokines engage new signaling programs with therapeutic potential.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Immunology

    Germinal center B cell development has distinctly regulated stages completed by disengagement from T cell help

    Ting-ting Zhang et al
    CD40 signaling is necessary to generate the immediate precursors of GC B cells, transition to the BCL6hi follicular state is promoted by a regional and transient diminution of T cell help.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Genomics and Evolutionary Biology

    Chimeric origins of ochrophytes and haptophytes revealed through an ancient plastid proteome

    Richard G Dorrell et al
    An in silico reconstruction of a chloroplast that existed hundreds of millions of years ago casts new insights in the evolutionary processes, endosymbioses and chimerism events that shape the origin of plastids.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells

    Intracellular uptake of macromolecules by brain lymphatic endothelial cells during zebrafish embryonic development

    Max van Lessen et al
    The discovery of a unique brain lymphatic cell type in the zebrafish model will facilitate the study of embryonic development and physiology, an essential mission to understand how clearance of macromolecules impact neurological diseases.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Computational and Systems Biology

    Kinetic modeling predicts a stimulatory role for ribosome collisions at elongation stall sites in bacteria

    Michael A Ferrin, Arvind R Subramaniam
    Collision between ribosomes can cause dissociation of stalled ribosomes from messenger RNAs during the process of mRNA translation.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Neuroscience

    A selectivity filter at the intracellular end of the acid-sensing ion channel pore

    Timothy Lynagh et al
    Sodium selectivity of acid-sensing ion channels is mediated by negatively charged side chains and not by a size-exclusion mechanism.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Ecology

    Digitizing mass spectrometry data to explore the chemical diversity and distribution of marine cyanobacteria and algae

    Tal Luzzatto-Knaan et al
    Mass spectrometry has potential as a tool for ecological studies and bioprospecting of natural products from marine cyanobacteria and algae.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells

    The Hippo pathway effector YAP is an essential regulator of ductal progenitor patterning in the mouse submandibular gland

    Aleksander D Szymaniak et al
    The transcriptional regulator YAP directs the specification and differentiation of stem cells that give rise to salivary gland ductal epithelium.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Immunology

    Na+ influx via Orai1 inhibits intracellular ATP-induced mTORC2 signaling to disrupt CD4 T cell gene expression and differentiation

    Yong Miao et al
    Non-specific sodium entry inhibits T cell receptor induced gene expression and differentiation of T cells by depleting intracellular ATP and disrupting mTORC2 dependent signalling axis.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells
    2. Neuroscience

    USP5/Leon deubiquitinase confines postsynaptic growth by maintaining ubiquitin homeostasis through Ubiquilin

    Chien-Hsiang Wang et al
    Maintenance of ubiquitin homeostasis is essential for proper control of the size of postsynaptic density and the growth of specialized membrane structure.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Noradrenaline blockade specifically enhances metacognitive performance

    Tobias U Hauser et al
    The neurotransmitter noradrenaline selectively modulates metacognition, the conscious insight into one's performance, but does not alter perceptual decision making, revealing that different neuromodulators affect different stages of a decision making process.
    Research Advance
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells
    2. Neuroscience

    Analogous mechanism regulating formation of neocortical basal radial glia and cerebellar Bergmann glia

    Xin Heng et al
    Genome-wide transcriptional profiling and genetic analyses reveal conserved mechanisms underlying the generation of neocortical basal radial glia and cerebellar Bergmann glia.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Reciprocal synapses between mushroom body and dopamine neurons form a positive feedback loop required for learning

    Isaac Cervantes-Sandoval et al
    Structural and functional analysis of axonal-axonal reciprocal connections between dopamine neurons and Kenyon cells provides insight into the brain computations for normal associative olfactory learning.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Odor identity coding by distributed ensembles of neurons in the mouse olfactory cortex

    Benjamin Roland et al
    In vivo calcium imaging reveals how odor identity is encoded in the activity of large, distributed ensembles of olfactory cortex neurons in mice.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genomics and Evolutionary Biology

    Homo naledi and Pleistocene hominin evolution in subequatorial Africa

    Lee R Berger et al
    A late Middle Pleistocene age for Homo naledi demonstrates a diversity of hominin species in Africa at this critical time in the archaeological record.
    Short Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genomics and Evolutionary Biology

    New fossil remains of Homo naledi from the Lesedi Chamber, South Africa

    John Hawks et al
    The discovery of new skeletal remains of Homo naledi in the Lesedi Chamber, South Africa, adds more evidence to our understanding of the morphology and behavior of this recently discovered species.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genomics and Evolutionary Biology
    2. Microbiology and Infectious Disease

    Evolution of reduced co-activator dependence led to target expansion of a starvation response pathway

    Bin Z He et al
    The phosphate starvation response network in a commensal yeast evolved to expand its downstream targets via changes in the main transcription factor's dependence on its co-activator, potentially altering the physiological response.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genes and Chromosomes

    Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes

    Yanxiang Ni et al
    Molecular beacon based FISH shows the capability of visualizing a 2.5 kb non-repetitive genomic DNA sequence in situ in human or mouse nuclear genome at super resolution.
    Tools and Resources
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    MCTP is an ER-resident calcium sensor that stabilizes synaptic transmission and homeostatic plasticity

    Özgür Genç et al
    MCTP is a novel presynaptic calcium sensor, resident within the endoplasmic reticulum, that is required for normal baseline neurotransmission, short-term synaptic plasticity and presynaptic homeostatic plasticity.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Microbiology and Infectious Disease

    Reproducible diagnostic metabolites in plasma from typhoid fever patients in Asia and Africa

    Elin Näsström et al
    Mass spectrometry on plasma from patients with typhoid fever and other febrile disease identified and validated 24 metabolites that can distinguish typhoid from other febrile diseases, providing a new approach for typhoid diagnostics.
    Research Advance
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genomics and Evolutionary Biology

    The age of Homo naledi and associated sediments in the Rising Star Cave, South Africa

    Paul HGM Dirks et al
    Independent dating techniques have established that the H. naledi fossils are between 236 and 335 thousand years old, indicating that small-brained hominins with relatively primitive body shapes co-existed with our early ancestors in Africa.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Ecology
    2. Microbiology and Infectious Disease

    A diverse host thrombospondin-type-1 repeat protein repertoire promotes symbiont colonization during establishment of cnidarian-dinoflagellate symbiosis

    Emilie-Fleur Neubauer et al
    The colonization of corals and their relatives by intracellular microalgae is facilitated by immunity proteins in the animal that contain thrombospondin-type-1 repeats, elucidating the inter-partner recognition processes required for the establishment of this ecologically important symbiosis.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Computational and Systems Biology

    Functional mapping of yeast genomes by saturated transposition

    Agnès H Michel et al
    A new method maps functional and structural features of yeast genomes with unprecedented ease and throughput, which allows identification of protein domains at the genome scale.
    Tools and Resources
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Learning multiple variable-speed sequences in striatum via cortical tutoring

    James M Murray, G Sean Escola
    Inspired by the sparse, sequential neural activity patterns observed in striatum, a new circuit model implements variable-speed activity, the encoding of multiple sequences, and a tutor/student relationship between cortex and striatum.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Multiple short windows of calcium-dependent protein kinase 4 activity coordinate distinct cell cycle events during Plasmodium gametogenesis

    Hanwei Fang et al
    CDPK4 is a pleiotropic regulator controlling initiation of DNA replication, mitotic spindle assembly and flagellar activation during the early stages of Plasmodium transmission.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology

    Metaphase chromosome structure is dynamically maintained by condensin I-directed DNA (de)catenation

    Ewa Piskadlo et al
    Condensin I maintains chromosome organization throughout metaphase by preventing erroneous topoisomerase II-dependent sister chromatid re-entanglements.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Neuroscience

    Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion

    Ewa Sitarska et al
    Biophysical and functional data strongly support the notion that Munc18-1 acts as a template to assemble the neuronal SNARE complex, and that inhibition of this activity underlies diverse forms of regulation of neurotransmitter release.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Shank is a dose-dependent regulator of Cav1 calcium current and CREB target expression

    Edward Pym et al
    Changes in Shank gene dosage alter voltage-activated calcium current and calcium-activated gene expression in a manner that parallels the effects of human Shank copy number variation on psychiatric disease risk.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry

    SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function

    Kate E Coleman et al
    Aberrant neddylation of non-cullin substrates disrupts normal cell cycle progression.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells

    Resolving stem and progenitor cells in the adult mouse incisor through gene co-expression analysis

    Kerstin Seidel et al
    Gene co-expression analysis identifies coherent transcriptional patterns driven by distinct cell types in the mouse incisor, and functional studies of candidate genes reveal how the tissues are maintained through stem cell-fueled renewal.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Computational and Systems Biology
    2. Ecology

    Microbial consortia at steady supply

    Thibaud Taillefumier et al
    In a consumer-resource model obeying the physical requirement of flux conservation, metabolic competition between microbes yields consortia of cell types that collectively resist invasion via optimal use of resources.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Peroxisomal dysfunctions cause lysosomal storage and axonal Kv1 channel redistribution in peripheral neuropathy

    Sandra Kleinecke et al
    Dysfunctions of myelin peroxisomes cause a lysosomal storage-like disorder associated with alterations in glial and axonal membranes, which is the likely cause of nerve impairment in peroxisomal disorders.
    Short Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Cell Biology

    Single-protein detection in crowded molecular environments in cryo-EM images

    J Peter Rickgauer et al
    High-resolution template matching makes small, densely embedded proteins detectable.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Beta band oscillations in motor cortex reflect neural population signals that delay movement onset

    Preeya Khanna, Jose M Carmena
    Using a sequential neurofeedback-arm reaching task, a new link is established among population neural activity patterns, generation of beta oscillations, and motor behavior changes.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genomics and Evolutionary Biology

    Introduction of a male-harming mitochondrial haplotype via ‘Trojan Females’ achieves population suppression in fruit flies

    Jonci Nikolai Wolff et al
    Mitochondrial genomes harbor male-fertility-reducing mutations that can be harnessed to control population viability as a novel approach to control economic and environmental pests.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cancer Biology
    2. Cell Biology

    Regulation of localization and function of the transcriptional co-activator YAP by angiomotin

    Susana Moleirinho et al
    Phosphorylation of Angiomotin regulates YAP localization and activity.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology
    2. Genes and Chromosomes

    CTCF and cohesin regulate chromatin loop stability with distinct dynamics

    Anders S Hansen et al
    Single-molecule imaging of CTCF and cohesin in live cells suggests that chromatin loops are dynamic structures that frequently form and fall apart.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cancer Biology
    2. Cell Biology

    TCF7L1 promotes skin tumorigenesis independently of β-catenin through induction of LCN2

    Amy T Ku et al
    Overexpression of TCF7L1 overrides oncogenic Ras-induced senescence, induces cell migration, and promotes growth of skin squamous cell carcinoma independently of its interaction with β-catenin.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Developmental Biology and Stem Cells

    Loss of Ptpn11 (Shp2) drives satellite cells into quiescence

    Joscha Griger et al
    The stem cells of the postnatal muscle allow postnatal muscle growth and repair and withdraw from the cell cycle when the tyrosine phosphatase Ptpn11 (Shp2) is inhibited or mutated in mice.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genomics and Evolutionary Biology
    2. Microbiology and Infectious Disease

    Adaptive tuning of mutation rates allows fast response to lethal stress in Escherichia coli

    Toon Swings et al
    Population mutation rates are highly flexible and evolvable under extreme stress conditions, matching changes in selective pressure to avoid extinction of the entire population.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Computational and Systems Biology
    2. Neuroscience

    Saccadic suppression as a perceptual consequence of efficient sensorimotor estimation

    Frédéric Crevecoeur, Konrad P Kording
    Neural computations necessary for efficient control of saccades capture the phenomenon of saccadic suppression, which suggests that neural resources are shared for perception and control.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Ecology
    2. Genomics and Evolutionary Biology

    Transcriptome analysis illuminates the nature of the intracellular interaction in a vertebrate-algal symbiosis

    John A Burns et al
    The endosymbiosis between an alga and the spotted salamander shows several parallels to invertebrate-algal symbioses as well as to pathogen associations in vertebrate animals.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology

    Nuclear export of misfolded SOD1 mediated by a normally buried NES-like sequence reduces proteotoxicity in the nucleus

    Yongwang Zhong et al
    Cellular and genetic approaches reveal that exposure of a normally buried nuclear export signal (NES)-like sequence mediates export of ALS-linked mutant and misfolded wild-type SOD1 to the cytoplasm by CRM1.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Neuroscience

    A novel ALS-associated variant in UBQLN4 regulates motor axon morphogenesis

    Brittany M Edens et al
    A novel ALS-associated variant in UBQLN4 impairs proteasome function and beta-catenin degradation to drive aberrant axon morphogenesis in motor neurons.
    Short Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Genes and Chromosomes
    2. Human Biology and Medicine

    Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering

    Kunwoo Lee et al
    The guide RNA and donor DNA of the CRISPR/Cas system tolerate large chemical modifications and can be engineered for enhanced delivery and gene editing.
    Short Report
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    Decreased microRNA levels lead to deleterious increases in neuronal M2 muscarinic receptors in Spinal Muscular Atrophy models

    Patrick J O'Hern et al
    In invertebrate and vertebrate models of Spinal Muscular Atrophy, diminished SMN protein causes Gemin3-dependent decreases in microRNA function, leading to upregulated M2 muscarinic receptor and deleterious consequences.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology

    PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling

    Stefania Monterisi et al
    The enzyme phosphodiesterase 2A2 localises at the mitochondrial membrane and its inhibition results in a local increase in cAMP concentration, mitochondrial elongation and resistance to apoptosis.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Biophysics and Structural Biology

    Control of lipid domain organization by a biomimetic contractile actomyosin cortex

    Sven Kenjiro Vogel et al
    Building on previous work (Vogel et al., 2013b), it is shown that myosin-driven actin filament rearrangements actively move, split or fuse individual lipid domains and change their overall shape.
    Research Advance
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Plant Biology

    RISC-interacting clearing 3’- 5’ exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thaliana

    Zhonghui Zhang et al
    AGO protein-interacting exoribonucleases clear RNA induced silencing complex (RISC)-resulting 5' cleavage products in time to recycle RISC and miRNAs.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Neuroscience

    The role of PDF neurons in setting the preferred temperature before dawn in Drosophila

    Xin Tang et al
    In Drosophila, key neurons controlling sleep play an important role in adjusting the temperature set-point before dawn via time-dependent neuronal plasticity within the clock circuits.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology
    2. Developmental Biology and Stem Cells

    The Sec14-like phosphatidylinositol transfer proteins Sec14l3/SEC14L2 act as GTPase proteins to mediate Wnt/Ca2+ signaling

    Bo Gong et al
    Sec14l3/SEC14L2 respond to upstream Wnt/Frizzled/Dvl stimulation to recruit and activate phospholipase Cδ4a (Plcδ4a) to further initiate calcium release.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Cell Biology

    Protein Phosphatase 1 inactivates Mps1 to ensure efficient Spindle Assembly Checkpoint silencing

    Margarida Moura et al
    Inactivation of the master mitotic checkpoint regulator Mps1 by protein phosphatase 1 is required for timely segregation of the genetic material during cell division.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf

Magazine

    1. Biophysics and Structural Biology

    Ion Channels: Poring over furrows

    Skylar ID Fisher, H Criss Hartzell
    1. Neuroscience

    Thermoregulation: How a brain keeps its cool

    Swathi Yadlapalli, Orie T Shafer
    1. Neuroscience

    Chemoreception: Keeping carbon dioxide in check

    Alfredo J Garcia III, Jan-Marino Ramirez