December 2023

Research articles

1. 1. Physics of Living Systems

   IS200/IS605 Family-Associated TnpB Increases Transposon Activity and Retention

   Davneet Kaur, Thomas E. Kuhlman
2. 1. Genetics and Genomics
   2. Evolutionary Biology

   Inversions Can Accumulate Balanced Sexual Antagonism: Evidence from Simulations and Drosophila Experiments

   Christopher S McAllester, John E Pool
3. 1. Neuroscience

   The neural and physiological substrates of real-world attention change across development.

   Marta Perapoch Amadó, Emily Greenwood ... Sam V. Wass
4. 1. Epidemiology and Global Health

   Measuring changes in Plasmodium falciparum census population size in response to sequential malaria control interventions

   Kathryn E Tiedje, Qi Zhan ... Karen P Day
5. 1. Microbiology and Infectious Disease

   Akkermansia muciniphila identified as key strain to alleviate gut barrier injury through Wnt signaling pathway

   Xin Ma, Li Meng ... Xinyan Han
6. 1. Medicine
   2. Stem Cells and Regenerative Medicine

   Iron chelation improves ineffective erythropoiesis and iron overload in myelodysplastic syndrome mice

   Wenbin An, Maria Feola ... Yelena Ginzburg

   Dysregulated erythroblast-specific iron trafficking and regulation of iron metabolism provides evidence of a novel potential therapeutic target to reverse ineffective erythropoiesis in MDS.
7. 1. Ecology
   2. Genetics and Genomics

   Non-invasive real-time genomic monitoring of the critically endangered kākāpō

   Lara Urban, Allison K Miller ... Andrew Digby

   The application of real-time genomics by long-read nanopore sequencing to environmental samples creates a new trajectory for non-invasive genomics-based monitoring of critically endangered wildlife.
8. 1. Genetics and Genomics
   2. Neuroscience

   Acute avoidance of hydrogen sulfide is modulated by external and internal states in C. elegans

   Longjun Pu, Lina Zhao ... Changchun Chen
9. 1. Medicine
   2. Neuroscience

   Enhanced K_Na1.1 Channel Underlies Cortical Hyperexcitability and Seizure Susceptibility after Traumatic Brain Injury

   Ru Liu, Lei Sun ... Jianping Wu
10. 1. Cell Biology
    2. Physics of Living Systems

    A high-throughput platform for single-molecule tracking identifies drug interaction and cellular mechanisms

    David T. McSwiggen, Helen Liu ... Hilary P. Beck
11. 1. Developmental Biology

    BMP signalling facilitates transit amplification in the developing chick and human cerebellum

    Victoria Rook, Parthiv Haldipur ... Richard J Wingate
12. 1. Developmental Biology
    2. Medicine

    Vertical transmission of maternal DNA through extracellular vesicles associates with altered embryo bioenergetics during the periconception period

    David Bolumar, Javier Moncayo-Arlandi ... Felipe Vilella

    In vitro analyses reveal how vertical DNA transmission from the mother to the pre-implantation embryo via endometrial extracellular vesicles can impact embryo bioenergetics.
13. 1. Genetics and Genomics
    2. Neuroscience

    Sensory neurons couple arousal and foraging decisions in Caenorhabditis elegans

    Elias Scheer, Cornelia I Bargmann

    A long-term arousal state is linked to the acute decision to leave a food patch by sensory neurons that integrate neuromodulatory information, food intake, and environmental signals.
14. 1. Neuroscience

    Courtship behaviour reveals temporal regularity is a critical social cue in mouse communication

    Catherine Perrodin, Colombine Verzat, Daniel Bendor

    A behavioural paradigm demonstrates that during courtship song preference by female mice relies on the temporal regularity of the male's production of song syllables.
15. 1. Evolutionary Biology
    2. Genetics and Genomics

    Efficient estimation for large-scale linkage disequilibrium patterns of the human genome

    Xin Huang, Tian-Neng Zhu ... Guo-Bo Chen

    Leveraging the X-LD method for estimating inter-chromosomal linkage disequilibrium in the human genome uncovers a universal extended LD pattern, consistent with observations in 1KG cohorts.
16. 1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Metabolic memory of Δ9-tetrahydrocannabinol exposure in pluripotent stem cells and primordial germ cells-like cells

    Roxane Verdikt, Abigail A Armstrong ... Patrick Allard

    Exposure of embryonic stem cells to physiological concentrations of Δ9-THC remodels cellular metabolism across differentiation.
17. 1. Biochemistry and Chemical Biology

    Unveiling the domain-specific and RAS isoform-specific details of BRAF kinase regulation

    Tarah Elizabeth Trebino, Borna Markusic ... Zhihong Wang

    An in-depth binding profile analysis of BRAF domains related to RAF activation and autoinhibition unveils the distinctive roles of each domain in selecting preferred RAS isoforms and facilitating autoinhibition.
18. 1. Cell Biology
    2. Physics of Living Systems

    Mechanical coupling coordinates microtubule growth

    Bonnibelle K Leeds, Katelyn F Kostello ... Charles L Asbury

    Microtubules grow at highly variable rates, but they can be tightly coordinated merely by coupling their tips to a single shared load.
19. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Mitochondrial electron transport chain, ceramide, and coenzyme Q are linked in a pathway that drives insulin resistance in skeletal muscle

    Alexis Diaz-Vegas, Søren Madsen ... James G Burchfield

    In vitro and in vivo studies uncovered a molecular link between elevated mitochondrial ceramide levels, coenzyme Q depletion, and insulin resistance.
20. 1. Neuroscience

    FSHβ links photoperiodic signaling to seasonal reproduction in Japanese quail

    Gaurav Majumdar, Timothy A Liddle ... Tyler Stevenson

    Molecular analyses reveal the neuroendocrine control of seasonal life-history transitions in birds.
21. 1. Neuroscience

    Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells

    John A Gaynes, Samuel A Budoff ... Alon Poleg-Polsky

    A combination of glutamate imaging, multicompartmental modeling, and machine learning elucidates the peak theoretical and actual contributions of heterogeneous kinetics of presynaptic inputs to the computation of directional selectivity in retinal starburst amacrine cells.
22. 1. Developmental Biology
    2. Microbiology and Infectious Disease

    Identification of key yeast species and microbe–microbe interactions impacting larval growth of Drosophila in the wild

    Ayumi Mure, Yuki Sugiura ... Yukako Hattori

    The growth of wild Drosophila larvae on fruits is promoted by a yeast releasing essential nutrients extracellularly or by a stable association with a nutrient-providing bacterium established by microbe–microbe interactions.
23. 1. Medicine

    Effects of neoadjuvant stereotactic body radiotherapy plus adebrelimab and chemotherapy for triple-negative breast cancer: A pilot study

    Guanglei Chen, Xi Gu ... Caigang Liu

    Stereotactic body radiotherapy combined with immunotherapy and chemotherapy in neoadjuvant setting exhibited a promising anti-tumor activity and an acceptable safety profile in triple-negative breast cancer patients.
24. 1. Immunology and Inflammation
    2. Stem Cells and Regenerative Medicine

    Maresin 1 repletion improves muscle regeneration after volumetric muscle loss

    Jesus A Castor-Macias, Jacqueline A Larouche ... Carlos A Aguilar

    The treatment of injury from volumetric muscle loss with Maresin 1 reduces inflammation and fibrosis, driving improvements in the recovery of muscle strength.
25. 1. Computational and Systems Biology
    2. Neuroscience

    BrainPy, a flexible, integrative, efficient, and extensible framework for general-purpose brain dynamics programming

    Chaoming Wang, Tianqiu Zhang ... Si Wu

    BrainPy presents a general-purpose programming framework that enables efficient implementation, simulation, training, and analysis of brain dynamics models across multiple organization scales, ultimately facilitating the understanding of the complex neural mechanisms underlying brain functions.
26. 1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    A computational method for predicting the most likely evolutionary trajectories in the stepwise accumulation of resistance mutations

    Ruth Charlotte Eccleston, Emilia Manko ... Nicholas Furnham

    A computational method for predicting evolutionary pathways to antimicrobial resistance, accounting for how epistatic interactions determine trajectories, is described.
27. 1. Neuroscience

    Imaging microglia surveillance during sleep-wake cycles in freely behaving mice

    Xiaochun Gu, Zhong Zhao ... Heping Cheng

    Using long-term in vivo imaging with miniature two-photon microscopy, microglia surveillance is sleep state-dependent and stress-induced in freely behaving mice, regulated by the locus coeruleus-norepinephrine system and β₂-adrenergic receptors signaling.
28. 1. Evolutionary Biology
    2. Genetics and Genomics

    Chromosome-level genome assembly of hadal snailfish reveals mechanisms of deep-sea adaptation in vertebrates

    Wenjie Xu, Chenglong Zhu ... Kun Wang

    The hadal snailfish rapidly evolved and adapted to extreme deep-sea environments, modifying crucial genes and enhancing tolerance to high-hydrostatic pressure through ferritin gene duplication.
29. 1. Neuroscience

    PAK3 downregulation induces cognitive impairment following cranial irradiation

    Haksoo Lee, Hyunkoo Kang ... BuHyun Youn

    p21-activated kinase 3 (PAK3) is a factor related to radiation-induced cognitive decline, and it has been found that regulating it leads to the recovery of cognitive decline induced by radiation.
30. 1. Structural Biology and Molecular Biophysics

    How accurately can one predict drug binding modes using AlphaFold models?

    Masha Karelina, Joseph J Noh, Ron O Dror

    AlphaFold 2 models capture binding pocket structures accurately but fare poorly when used to predict ligand-binding poses.
31. 1. Neuroscience

    Microglia facilitate and stabilize the response to general anesthesia via modulating the neuronal network in a brain region-specific manner

    Yang He, Taohui Liu ... Bo Peng

    Microglia differentially modulate the neuronal activities via P2Y12 and its downstream Ca²⁺ signaling in a brain region-specific manner, which facilitates and stabilizes the response to general anesthesia.
32. 1. Computational and Systems Biology
    2. Neuroscience

    Interpersonal alignment of neural evidence accumulation to social exchange of confidence

    Jamal Esmaily, Sajjad Zabbah ... Bahador Bahrami

    A multidisciplinary approach sheds light on the fundamental mechanisms underlying social belief communication under uncertainty.
33. 1. Cell Biology
    2. Immunology and Inflammation

    Apoptosis recognition receptors regulate skin tissue repair in mice

    Olivia Justynski, Kate Bridges ... Valerie Horsley

    After an injury to the skin, murine wounds upregulate apoptosis and efferocytosis pathways, which are required for effective healing and may similarly impact wound healing in diabetic patients.
34. 1. Evolutionary Biology
    2. Genetics and Genomics

    The seminal odorant binding protein Obp56g is required for mating plug formation and male fertility in Drosophila melanogaster

    Nora C Brown, Benjamin Gordon ... Mariana Federica Wolfner

    A functional genetics approach reveals a novel role for odorant binding proteins in post-mating processes, with complex mechanisms of evolutionary change across closely related insect species.
35. 1. Microbiology and Infectious Disease
    2. Structural Biology and Molecular Biophysics

    Emerging variants of SARS-CoV-2 NSP10 highlight strong functional conservation of its binding to two non-structural proteins, NSP14 and NSP16

    Huan Wang, Syed RA Rizvi ... Shozeb Haider

    Structure and simulations reveal SARS-CoV-2 NSP10 is more resistant to genetic variations than other SARS-CoV-2 NSPs and that the presence of mutations conserves structural and dynamic changes in NSP10.
36. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Pharmacological inhibition of CLK2 activates YAP by promoting alternative splicing of AMOTL2

    Maya L Bulos, Edyta M Grzelak ... Michael J Bollong

    Screening repurposed drugs identifies that a clinical stage inhibitor splicing related kinases unexpectedly activates YAP driven transcription in cells.
37. 1. Cancer Biology
    2. Epidemiology and Global Health

    Identifying metabolic features of colorectal cancer liability using Mendelian randomization

    Caroline Bull, Emma Hazelwood ... Emma E Vincent

    Increased genetic liability to colorectal cancer is associated with altered levels of circulating metabolites, including fatty acids, up to 40 years before average age of diagnosis.
38. 1. Immunology and Inflammation

    The lncRNA Malat1 inhibits miR-15/16 to enhance cytotoxic T cell activation and memory cell formation

    Benjamin D Wheeler, John D Gagnon ... K Mark Ansel

    A single microRNA-binding site in a long non-coding RNA alters T cell responses in vivo by 'sponging' the miRNA to inhibit its gene regulatory function.
39. 1. Neuroscience

    CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice

    Jenny Clarkson, Siew Hoong Yip ... Allan E Herbison

    Preoptic area neurons co-expressing GABA and kisspeptin are essential for the preovulatory surge mechanism but not for the pulsatile secretion of luteinizing hormone.
40. 1. Neuroscience

    Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology

    Xiling Bian, Jiemin Zhu ... Yi Rao

    While neurotransmitters were discovered decades ago, evidence suggests that more transmitters are to be discovered using the presented approaches.
41. 1. Immunology and Inflammation

    CTLA-4 antibody-drug conjugate reveals autologous destruction of B-lymphocytes associated with regulatory T cell impairment

    Musleh M Muthana, Xuexiang Du ... Yang Liu

    A biochemical probe targeting Tregs shows the importance of Tregs for B cell homeostasis and peripheral B cell reduction is caused by an activated T-cell-dependent mechanism.
42. 1. Developmental Biology
    2. Genetics and Genomics

    Identification of CFAP52 as a novel diagnostic target of male infertility with defects of sperm head-tail connection and flagella development

    Hui-Juan Jin, Tiechao Ruan ... Su-Ren Chen

    CFAP52 is a candidate for genetic diagnosing male infertility with defects of sperm head-tail connection and flagella development.
43. 1. Cancer Biology

    Single-cell characterization of human GBM reveals regional differences in tumor-infiltrating leukocyte activation

    Philip Schmassmann, Julien Roux ... Gregor Hutter

    A regionally resolved single-cell RNA-sequencing analysis of GBM-associated leukocytes reveals distinct activation states of the main immune populations between tumor center, peripheral infiltration zone and blood.
44. 1. Immunology and Inflammation

    Commensal bacteria maintain a Qa-1^b-restricted unconventional CD8⁺ T population in gut epithelium

    Jian Guan, J David Peske ... Scheherazade Sadegh-Nasseri

    Analysis of conventional and gnotobiotic mice showed that a MHC-E-restricted unconventional CD8 T cell population is retained in the small intestine epithelium by a commensal bacterium.
45. 1. Chromosomes and Gene Expression

    Adenine methylation is very scarce in the Drosophila genome and not erased by the ten-eleven translocation dioxygenase

    Manon Boulet, Guerric Gilbert ... Lucas Waltzer

    Biochemical, molecular, and genetic analyses show that in Drosophila the epigenetic enzyme TET does not demethylate 6mA, which is scarce in the genome, but rather acts in a catalytic-independent manner.
46. 1. Plant Biology

    Soybean RIN4 represents a mechanistic link between plant immune and symbiotic signaling

    Katalin Tóth, Daewon Kim ... Gary Stacey
47. 1. Neuroscience

    Reduced discrimination between signals of danger and safety but not overgeneralization is linked to exposure to childhood adversity in healthy adults

    Maren Klingelhöfer-Jens, Katharina Hutterer ... Tina B Lonsdorf
48. 1. Computational and Systems Biology

    Predicting the effect of CRISPR-Cas9-based epigenome editing

    Sanjit Singh Batra, Alan Cabrera ... Yun S Song
49. 1. Developmental Biology
    2. Physics of Living Systems

    Core PCP mutations affect short-time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing

    Romina Piscitello-Gómez, Franz S Gruber ... Suzanne Eaton

    Quantitative analysis of cell dynamics over a range of timescales reveals that core PCP is not required to organize large-scale tissue flows but does affect short timescale mechanics.
50. 1. Neuroscience

    Transcranial focused ultrasound to human rIFG improves response inhibition through modulation of the P300 onset latency

    Justin M Fine, Archana S Mysore ... Marco Santello

    Ultrasonic neurostimulation in humans can manipulate response inhibition related behavior and neural activity.
51. 1. Neuroscience

    Movies reveal the fine-grained organization of infant visual cortex

    Cameron T Ellis, Tristan S Yates ... Nicholas B Turk-Browne
52. 1. Immunology and Inflammation

    Synovial macrophage diversity and activation of M-CSF signaling in post-traumatic osteoarthritis

    Alexander J. Knights, Easton C. Farrell ... Tristan Maerz
53. 1. Biochemistry and Chemical Biology
    2. Ecology

    Binding and sequestration of poison frog alkaloids by a plasma globulin

    Aurora Alvarez-Buylla, Marie-Therese Fischer ... Lauren A O'Connell

    Alkaloid-binding globulin (ABG) is a novel protein discovered in the plasma of poison frogs that transports many alkaloids, with structural homology to mammalian hormone carriers and amphibian biliverdin-binding serpin.
54. 1. Neuroscience

    Myomatrix arrays for high-definition muscle recording

    Bryce Chung, Muneeb Zia ... Samuel J Sober

    A novel technology for recording muscle activity provides unprecedented access to the electrical signals that control the body across muscles, species, and behaviors.
55. 1. Neuroscience

    Enabling brain-wide mapping of directed functional connectivity at 3T via layer-dependent fMRI with draining-vein suppression

    Wei-Tang Chang, Weili Lin, Kelly S. Giovanello
56. 1. Neuroscience

    The reuniens nucleus of the thalamus facilitates hippocampo-cortical dialogue during sleep

    Diellor Basha, Amirmohammad Azarmehri ... Igor Timofeev
57. 1. Neuroscience

    Respiratory and Cardiac Interoceptive Sensitivity in the First Two Years of Life

    Markus R Tünte, Stefanie Höhl ... Ezgi Kayhan
58. 1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Estimating the contribution of subclinical tuberculosis disease to transmission: An individual patient data analysis from prevalence surveys

    Jon C Emery, Peter J Dodd ... Rein MGJ Houben

    Data analysis and mathematical modelling suggest that subclinical tuberculosis contributes substantially to transmission and needs to be diagnosed and treated for effective progress towards tuberculosis elimination.
59. 1. Structural Biology and Molecular Biophysics

    Computational analysis of long-range allosteric communications in CFTR

    Ayca Ersoy, Bengi Altintel ... Oded Lewinson

    A combination of computational approaches identifies a novel allosteric hotspot in cystic fibrosis transmembrane conductance regulator.
60. 1. Developmental Biology
    2. Neuroscience

    Age-associated changes in lineage composition of the enteric nervous system regulate gut health and disease

    Subhash Kulkarni, Monalee Saha ... Pankaj Jay Pasricha

    Mesoderm-derived enteric neurons born in the post-natal vertebrates drive maturation and aging of the enteric nervous system.
61. 1. Neuroscience

    Functional and pharmacological analyses of visual habituation learning in larval zebrafish

    Laurie Anne Lamiré, Martin Haesemeyer ... Owen Randlett

    Complex and distributed plasticity processes are revealed by a functional and pharmacological analysis of visual habituation learning in larval zebrafish.
62. 1. Evolutionary Biology

    Major patterns in the introgression history of Heliconius butterflies

    Yuttapong Thawornwattana, Fernando Seixas ... James Mallet

    The full-likelihood multispecies coalescent approach is useful for studying species groups with high rates of introgression from whole-genome sequence data.
63. 1. Ecology
    2. Neuroscience

    Mouthparts of the bumblebee (Bombus terrestris) exhibit poor acuity for the detection of pesticides in nectar

    Rachel H Parkinson, Jennifer Scott ... Geraldine A Wright

    Bees are at risk of consuming harmful pesticides found in nectar because they cannot detect them using their mouthparts.
64. 1. Developmental Biology

    Opposing, spatially-determined epigenetic forces impose restrictions on stochastic olfactory receptor choice

    Elizaveta V Bashkirova, Nell Klimpert ... Stavros Lomvardas

    Dorsoventral gradients of transcription factors NFIA, B, and X impose epigenetic and transcriptional spatial restrictions to olfactory receptor expression.
65. 1. Microbiology and Infectious Disease

    Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy

    Benjamin Liffner, Ana Karla Cepeda Diaz ... Sabrina Absalon

    Ultrastructure expansion microscopy unlocks new fundamental cell biology of malaria parasites, providing new insights into processes including establishment of cell polarity, organelle biogenesis, and organelle fission.
66. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C

    Thomas Hansen, Jocelyn Lee ... Peter L Davies

    Self-assembly of bacterial ice nucleation proteins is required for efficient activity and is mediated by electrostatic interactions, creating long and thin fibres.
67. 1. Neuroscience

    Fear conditioning biases olfactory stem cell receptor fate

    Clara W. Liff, Yasmine R. Ayman ... Bianca Jones Marlin
68. 1. Neuroscience
    2. Computational and Systems Biology

    Multisensory integration operates on correlated input from unimodal transients channels

    Cesare V Parise, Marc O Ernst
69. 1. Neuroscience

    Silencing long-descending inter-enlargement propriospinal neurons improves hindlimb stepping after contusive spinal cord injuries

    Courtney T Shepard, Brandon L Brown ... David SK Magnuson

    Silencing long-descending propriospinal neurons that connect C6 to L2 disrupts right-left alternation of both the forelimbs and hindlimbs in the intact animal, but leads to improved stepping after a mild-moderate thoracic contusion spinal cord injury.
70. 1. Neuroscience

    Action does not enhance but attenuates predicted touch

    Xavier Job, Konstantina Kilteni

    A series of pre-registered psychophysical studies on self-touch tested two opposing models of somatosensory attenuation (cancelation) and enhancement (sharpening) regarding how action affects perception, and revealed that action prediction produces an attenuation, and not an enhancement, of the predicted touch.
71. 1. Cancer Biology
    2. Genetics and Genomics

    Single-cell sequencing highlights heterogeneity and malignant progression in actinic keratosis and cutaneous squamous cell carcinoma

    Dan-Dan Zou, Ya-Zhou Sun ... Xin Li

    A single-cell RNA profiling analysis has delineated the molecular changes driving the progression from actinic keratosis to cutaneous squamous cell carcinoma.
72. 1. Neuroscience

    Serial attentional resource allocation during parallel feature value tracking

    Christian Merkel, Luise Burgmann ... Jens-Max Hopf

    The strong limitation of human subjects in tracking two color streams simultaneously as they independently traverse color space can be attributed to attention alternating slowly and sequentially between streams.
73. 1. Cell Biology
    2. Neuroscience

    Trabid patient mutations impede the axonal trafficking of adenomatous polyposis coli to disrupt neurite growth

    Daniel Frank, Maria Bergamasco ... Hoanh Tran

    A novel DUB-regulated mechanism controls polarized axon growth and guidance.
74. 1. Cancer Biology
    2. Cell Biology

    Distinct states of nucleolar stress induced by anticancer drugs

    Tamara A Potapova, Jay R Unruh ... Jennifer L Gerton

    Anticancer compound library screen identified many types of nucleolar stress and revealed an essential role of transcriptional cyclin-dependent kinases in nucleolar structure and function.
75. 1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    The reciprocal regulation between mitochondrial-associated membranes and Notch signaling in skeletal muscle atrophy

    Yurika Ito, Mari Yamagata ... Takahiko Sato

    The maintenance of proper mitochondrial-associated endoplasmic reticulum membranes is crucial for preventing skeletal muscle atrophy to interact with the Notch signaling pathway.
76. 1. Cell Biology
    2. Developmental Biology

    Zn²⁺ is essential for Ca²⁺ oscillations in mouse eggs

    Hiroki Akizawa, Emily M Lopes, Rafael A Fissore

    Throughout oocyte maturation, there is a simultaneous elevation in the concentrations of Ca²⁺ and Zn²⁺, and the fertilization-induced Ca²⁺ oscillations cease under Zn²⁺-deficient conditions, implying an interplay between these ions, potentially at the IP₃R1 level that harbors a Zn²⁺-binding domain.
77. 1. Cell Biology

    GTPase activating protein DLC1 spatio-temporally regulates Rho signaling

    Max Heydasch, Lucien Hinderling ... Olivier Pertz
78. 1. Neuroscience

    Postsynaptic cell type and synaptic distance do not determine efficiency of monosynaptic rabies virus spread measured at synaptic resolution

    Maribel Patiño, Willian N Lagos ... Edward M Callaway

    Under typical monosynaptic rabies tracing conditions about 40% of first-order presynaptic synapses to cortical neurons are labeled and spread efficiency does not vary depending on the distance of synapses to the cell body, across dendrite types, or starter neuron types.
79. 1. Developmental Biology

    Neural tube-associated boundary caps are a major source of mural cells in the skin

    Gaspard Gerschenfeld, Fanny Coulpier ... Piotr Topilko

    Neural tube-associated boundary caps are heterogeneous with a non-neural crest-derived subpopulation that may originate from the developing meninges, which gives rise to derivatives that migrate along nerves to detach and differentiate into skin mural cells.
80. 1. Developmental Biology

    Steroidogenesis and androgen/estrogen signaling pathways are altered in in vitro matured testicular tissues of prepubertal mice

    Laura Moutard, Caroline Goudin ... Christine Rondanino

    Although complete mouse in vitro spermatogenesis can be achieved, failure of adult Leydig cell development, with accumulation of progesterone and estradiol, androstenedione deficiency, deregulation of steroidogenic, and steroid target gene expression, were highlighted in organotypic cultures, even with hCG.
81. 1. Evolutionary Biology

    Repeatability of adaptation in sunflowers reveals that genomic regions harbouring inversions also drive adaptation in species lacking an inversion

    Shaghayegh Soudi, Mojtaba Jahani ... Sam Yeaman

    Adaptation to climate in sunflowers is highly polygenic, but still involves significant repeatability with many of the same genes being used by multiple species.
82. 1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes

    Nicole A Ellis, Kevin S Myers ... Matthias P Machner

    A randomized CRISPR-based gene silencing approach overcomes functional redundancy and discovers virulence-critical gene combinations in the pathogen Legionella pneumophila.
83. 1. Cell Biology
    2. Developmental Biology

    Ulk4 promotes Shh signaling by regulating Stk36 ciliary localization and Gli2 phosphorylation

    Mengmeng Zhou, Yuhong Han, Jin Jiang

    Phosphorylation of the pseudokinase Ulk4 by Stk36 promotes primary ciliary tip localization of both proteins to facilitate the phosphorylation and activation of Gli in response to Sonic hedgehog.
84. 1. Physics of Living Systems

    An in silico testbed for fast and accurate MR labeling of orthopedic implants

    Gregory M Noetscher, Peter J Serano ... Sergey N Makaroff

    Radio frequency heating induced during magnetic resonance procedures within patients that carry embedded passive orthopedic implants may be accurately estimated through modeling and simulation.
85. 1. Genetics and Genomics

    Plasma growth hormone pulses induce male-biased pulsatile chromatin opening and epigenetic regulation in adult mouse liver

    Andy Rampersaud, Jeannette Connerney, David J Waxman

    Pulsatile chromatin opening stimulated by naturally-occurring plasma growth hormone pulses is one of two GH-determined mechanisms that establish widespread sex differences in hepatic chromatin accessibility and epigenetic regulation, both closely linked to sex differences in liver gene transcription and function.
86. 1. Cell Biology
    2. Genetics and Genomics

    LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility

    Jae Yeon Hwang, Pengxin Chai ... Jean-Ju Chung

    LRRC23 is a radial spoke 3 head component essential for normal sperm motility and fertility in mammals.
87. 1. Chromosomes and Gene Expression
    2. Computational and Systems Biology

    Genome-wide Functional Characterization of Escherichia coli Promoters and Sequence Elements Encoding Their Regulation

    Guillaume Urtecho, Kimberly D. Insigne ... Sriram Kosuri
88. 1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Cervical cancer screening improvements with self-sampling during the COVID-19 pandemic

    Miriam Elfström, Penelope Grace Gray, Joakim Dillner

    Improved cervical cancer prevention through greatly increased population coverage by use of self-sampling and HPV screening during the COVID-19 pandemic.
89. 1. Immunology and Inflammation
    2. Neuroscience

    Cystatin F (Cst7) drives sex-dependent changes in microglia in an amyloid-driven model of Alzheimer’s disease

    Michael JD Daniels, Lucas Lefevre ... Barry W McColl

    Cst7, a gene coding for protein cystatin F, is expressed by microglia in neurodegenerative disease models and mediates sex-dependent effects on microglial endolysosomal function and disease pathology.
90. 1. Neuroscience

    A reservoir of timescales emerges in recurrent circuits with heterogeneous neural assemblies

    Merav Stern, Nicolae Istrate, Luca Mazzucato

    The large range of timescales empirically observed in neural circuits can be naturally explained when neural assemblies of heterogeneous size are recurrently coupled, empowering the neural circuits to efficiently process complex time-varying input signals.
91. 1. Evolutionary Biology

    How hibernation in frogs drives brain and reproductive evolution in opposite directions

    Wenbo Liao, Ying Jiang ... Stefan Lüpold

    Rumation of anurans covaried negatively with brain size but positively with reproductive investment, likely in response to brumation-dependent changes in the socio-ecological context and associated selection on different tissues.
92. 1. Cell Biology

    N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters

    Anshuman Bhanja, Margaret K Seeley-Fallen ... Wenxia Song

    B-cells generate inner actomyosin foci from lamellipodial actin networks to drive cell contraction on antigen-presenting surfaces, which causes signaling molecule disassociation from B-cell receptor clusters and signal attenuation.
93. 1. Neuroscience

    Endosomal dysfunction contributes to cerebellar deficits in spinocerebellar ataxia type 6

    Anna A Cook, Tsz Chui Sophia Leung ... Alanna Jean Watt

    Changes in the intracellular trafficking of key signaling molecules in endosomes lead to pathophysiology in a rare disease affecting the cerebellum.
94. 1. Biochemistry and Chemical Biology

    Phosphorylation bar-coding of free fatty acid receptor 2 is generated in a tissue-specific manner

    Natasja Barki, Laura Jenkins ... Graeme Milligan

    Differential agonist-induced phosphorylation of free fatty acid receptor 2 is shown in different tissues of transgenic mice engineered to express variant forms of the human ortholog of the receptor.
95. 1. Computational and Systems Biology

    Knock-down of a regulatory barcode shifts macrophage polarization destination from M1 to M2 and increases pathogen burden upon S. aureus infection

    Sathyabaarathi Ravichandran, Bharat Bhatt ... Nagasuma Chandra
96. 1. Neuroscience

    A neural network model of hippocampal contributions to category learning

    Jelena Sučević, Anna C Schapiro

    A neural network model of the hippocampus exhibits a division of labor across its two main pathways during category learning, with one pathway specializing in extracting systematic category information and another in encoding arbitrary details.
97. 1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    Activin A marks a novel progenitor cell population during fracture healing and reveals a therapeutic strategy

    Lutian Yao, Jiawei Lu ... Maurizio Pacifici

    Activation of Activin A-expressing progenitor cells emerges as a pivotal mechanism in bone fracture healing, shedding light on a potential therapeutic avenue to augment bone repair processes.
98. 1. Neuroscience

    Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging

    Hirofumi Noguchi, Jessica Chelsea Arela ... Samuel Pleasure

    Hilar mossy cells control seizure-dependent dentate neurogenesis and stem cell quiescence by activity-dependent control of Sonic hedgehog production and signaling.
99. 1. Neuroscience

    Effort cost of harvest affects decisions and movement vigor of marmosets during foraging

    Paul Hage, In Kyu Jang ... Reza Shadmehr

    When the acquisition of reward becomes effortful, marmosets choose to work longer, delaying their harvest, but slow their movements, reducing energy consumption.
100. 1. Medicine

     Ryanodine receptor 2 inhibition reduces dispersion of cardiac repolarization, improves contractile function, and prevents sudden arrhythmic death in failing hearts

     Pooja Joshi, Shanea Estes ... Swati Dey

     Inhibition of RyR2 hyperactivity with dantrolene not only prevents VT/VF and SCD but also heart failure by mitigating calcium dysfunction in pressure-overloaded hearts.
101. 1. Cancer Biology

     A positive feedback loop between ZEB2 and ACSL4 regulates lipid metabolism to promote breast cancer metastasis

     Jiamin Lin, Pingping Zhang ... Na Yang

     The ZEB2/ACSL4 axis acts as a novel metastatic metabolic pathway that stimulates both lipogenesis and fatty acid oxidation, resulting in enhanced breast cancer invasion and metastasis.
102. 1. Biochemistry and Chemical Biology
     2. Cell Biology

     Mitochondrial temperature homeostasis resists external metabolic stresses

     Mügen Terzioglu, Kristo Veeroja ... Howard T Jacobs

     Based on two independent methods, mitochondria operate at temperatures up to 15°C hotter than ambient, adjusting their heat production so as to maintain temperature in face of physiological stresses.
103. 1. Computational and Systems Biology
     2. Neuroscience

     A computationally informed comparison between the strategies of rodents and humans in visual object recognition

     Anna Elisabeth Schnell, Maarten Leemans ... Hans Op de Beeck

     Using a computational approach for designing object recognition tasks is beneficial to highlight the different strategies among different species.
104. 1. Neuroscience

     OpenApePose, a database of annotated ape photographs for pose estimation

     Nisarg Desai, Praneet Bala ... Benjamin Hayden

     A dedicated dataset for non-human apes improves pose estimation and tracking accuracy in apes.
105. 1. Genetics and Genomics
     2. Medicine

     Allelic strengths of encephalopathy-associated UBA5 variants correlate between in vivo and in vitro assays

     Xueyang Pan, Albert N Alvarez ... Hugo J Bellen

     A comprehensive platform is established for the evaluation of both current and future individuals afflicted with the UBA5-associated developmental and epileptic encephalopathy.
106. 1. Computational and Systems Biology

     Assessing drug safety by identifying the axis of arrhythmia in cardiomyocyte electrophysiology

     Stewart Heitmann, Jamie I Vandenberg, Adam P Hill

     A novel mathematical method for screening new drugs for pro-arrhythmic cardiotoxicity without using animals or costly drug-specific computer simulations is proposed.
107. 1. Genetics and Genomics
     2. Immunology and Inflammation

     Nicotinic acetylcholine receptor signaling maintains epithelial barrier integrity

     Nadja S Katheder, Kristen C Browder ... Heinrich Jasper

     Signaling through nicotinic acetylcholine receptors in enterocytes impacts barrier integrity in the Drosophila intestine by regulating formation of the peritrophic matrix.
108. 1. Computational and Systems Biology

     Homeostasis, injury, and recovery dynamics at multiple scales in a self-organizing mouse intestinal crypt

     Louis Gall, Carrie Duckworth ... Carmen Pin

     Novel modelling strategies can integrate the dynamics of processes regulating the intestinal epithelium at multiple scales in homeostasis and following perturbations to provide unprecedented insights into the biology of the epithelium and support the development of safer novel drug candidates.
109. 1. Biochemistry and Chemical Biology
     2. Evolutionary Biology

     Evolution towards simplicity in bacterial small heat shock protein system

     Piotr Karaś, Klaudia Kochanowicz ... Krzysztof Liberek

     Phylogenetic and biochemical analysis reveals crucial role of two substitutions in the α–crystallin domain for development of new functionality of IbpA chaperone after the loss of paralogous IbpB in Erwiniaceae.
110. 1. Developmental Biology

     MAFB drives differentiation by permitting WT1 binding to podocyte specific promoters

     Filippo M. Massa, Fariba Jian-Motamedi ... Andreas Schedl
111. 1. Neuroscience

     Microprism-based two-photon imaging of the mouse inferior colliculus reveals novel organizational principles of the auditory midbrain

     Baher A Ibrahim, Yoshitaka Shinagawa ... Daniel A Llano
112. 1. Neuroscience

     Multiple NTS neuron populations cumulatively suppress food intake

     Weiwei Qiu, Chelsea R Hutch ... Darleen Sandoval

     Analyses reveal that multiple NTS neuron types act cumulatively in the suppression of feeding, that the abrogation of food intake by multiple non-aversive populations fails to promote aversive responses, and that there exist additional NTS populations that modulate food intake.
113. 1. Evolutionary Biology
     2. Genetics and Genomics

     A timeline of bacterial and archaeal diversification in the ocean

     Carolina A Martinez-Gutierrez, Josef C Uyeda, Frank O Aylward

     Phylogenomics reveals the timeline over which marine bacteria and archaea colonized the oceans and shows the geological context of their diversification.
114. 1. Computational and Systems Biology

     Shared and distinct pathways and networks genetically linked to coronary artery disease between human and mouse

     Zeyneb Kurt, Jenny Cheng ... Xia Yang

     Cross-species multiomics network modeling revealed tissue-specific regulatory genes, pathways, and networks shared between mouse and human or unique to each species for atherosclerosis and coronary artery disease.
115. 1. Cell Biology

     Hsf1 and the molecular chaperone Hsp90 support a ‘rewiring stress response’ leading to an adaptive cell size increase in chronic stress

     Samarpan Maiti, Kaushik Bhattacharya ... Didier Picard

     Human cells adapt to chronic mild stresses, such as slightly elevated temperature, by getting larger in a process that couples increased translation to increased cell size in an Hsp90-dependent manner.
116. 1. Neuroscience

     Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines

     Connon I Thomas, Melissa A Ryan ... Benjamin Scholl

     Correlated light and electron microscopy of cortical neurons reveals a local organization of mitochondria near dendritic spines with diverse functional properties.
117. 1. Genetics and Genomics
     2. Neuroscience

     Opposing chemosensory functions of closely related gustatory receptors

     Ji-Eun Ahn, Hubert Amrein

     Live imaging and taste behavior analyses show that related members of the Gr28 gene subfamily are expressed in largely non-overlapping sets of neurons and mediate opposing taste behaviors.
118. 1. Physics of Living Systems

     A unified approach to dissecting biphasic responses in cell signaling

     Vaidhiswaran Ramesh, J Krishnan

     In-built competing effects are present at different levels in signaling networks, starting from basic biochemical building blocks to the network level, and mathematical and computational analysis reveals when they give rise to biphasic responses and what the consequences are.
119. 1. Neuroscience

     Modeling apical and basal tree contribution to orientation selectivity in a mouse primary visual cortex layer 2/3 pyramidal cell

     Konstantinos-Evangelos Petousakis, Jiyoung Park ... Panayiota Poirazi

     Apical and basal dendrites contribute differently to single-neuron orientation selectivity, highlighting the impact of feedforward and feedback signal interactions.
120. 1. Neuroscience

     Adaptive biasing of action-selective cortical build-up activity by stimulus history

     Anke Braun, Tobias H Donner

     Prior expectations about the state of the sensory environment are dynamically updated in a context-dependent manner and selectively bias the build-up of human motor cortical activity during subsequent decisions.
121. 1. Immunology and Inflammation

     Discovery and characterization of cross-reactive intrahepatic antibodies in severe alcoholic hepatitis

     Ali Reza Ahmadi, Guang Song ... Zhaoli Sun

     Massive IgG and IgA antibody deposition in severe alcoholic hepatitis livers, targeting unique human proteins and exhibiting hepatocyte killing potential, suggests a role for cross-reactive anti-bacterial antibodies in severe alcoholic hepatitis pathogenesis.
122. 1. Neuroscience
     2. Structural Biology and Molecular Biophysics

     Dynamic landscape of the intracellular termini of acid-sensing ion channel 1a

     Megan M Cullinan, Robert C Klipp ... John R Bankston

     A novel FRET approach suggests that the intracellular termini of acid-sensing ion channel 1a do not form a complex at rest requiring a new hypothesis for ASIC1a involvement in stroke.
123. 1. Immunology and Inflammation
     2. Microbiology and Infectious Disease

     Apoptotic signaling clears engineered Salmonella in an organ-specific manner

     Taylor J Abele, Zachary P Billman ... Edward A Miao

     Clearance of engineered Salmonella which trigger regulated cell death is dependent upon the cellular 'bucket list,' which is determined both by cell death signaling pathway and by cell type infected.
124. 1. Genetics and Genomics
     2. Immunology and Inflammation

     Sustained store-operated calcium entry utilizing activated chromatin state leads to instability in iTregs

     Huiyun Lyu, Guohua Yuan ... Yan Shi

     Multidimensional study of signaling pathways and epigenomics investigates how TCR (T cell receptor) signaling and chromatin landscape interpaly and impact on Treg stability.
125. 1. Immunology and Inflammation

     Pulsed ultrasound promotes secretion of anti-inflammatory extracellular vesicles from skeletal myotubes via elevation of intracellular calcium level

     Atomu Yamaguchi, Noriaki Maeshige ... Hidemi Fujino

     Ultrasound promotes release of extracellular vesicles from cultured myotubes and elicits anti-inflammatory effects on macrophages, indicating the potential of ultrasound stimulation to muscle for anti-inflammatory purposes.
126. 1. Structural Biology and Molecular Biophysics

     Folding of prestin’s anion-binding site and the mechanism of outer hair cell electromotility

     Xiaoxuan Lin, Patrick R Haller ... Tobin R Sosnick

     Identification of Cl⁻-induced folding advances the field’s understanding of prestin’s unique voltage-sensing mechanism and its involvement in mammalian hearing sensation.
127. 1. Chromosomes and Gene Expression

     Alternative end-joining results in smaller deletions in heterochromatin relative to euchromatin

     Jacob M. Miller, Sydney Prange ... Irene Chiolo
128. 1. Genetics and Genomics

     A population modification gene drive targeting both Saglin and Lipophorin impairs Plasmodium transmission in Anopheles mosquitoes

     Emily I Green, Etienne Jaouen ... Eric Marois

     Strong reduction in malaria transmission by genetically modified mosquitoes is achieved in the laboratory using a dual gene drive that simultaneously propagates an antiparasitic factor and a parasite-detrimental mosquito mutation.
129. 1. Physics of Living Systems

     Universal length fluctuations of actin structures found in cells

     Aldric Rosario, Shane G. McInally ... Jane Kondev
130. 1. Biochemistry and Chemical Biology
     2. Computational and Systems Biology

     Death by a thousand cuts through kinase inhibitor combinations that maximize selectivity and enable rational multitargeting

     Ian R Outhwaite, Sukrit Singh ... Markus A Seeliger

     Combinations of inhibitors can more selectively inhibit individual or multiple protein kinases than single inhibitors.
131. 1. Neuroscience

     Associative memory neurons of encoding multi-modal signals are recruited by neuroligin-3-mediated new synapse formation

     Yang Xu, Tian-liang Cui ... Jin-Hui Wang

     The coactivity of cortical neurons in associative learning recruits them as associative memory cells based on their synapse interconnections by neuroligin-3-mediated synapse formation, which endorses the first order and the second order of associative memory.
132. 1. Neuroscience

     Network-level changes in the brain underlie fear memory strength

     Josue Haubrich, Karim Nader

     Fear memory intensity modulates brain connectivity patterns, with stronger memories causing a pronounced reduction in interregional coordinated activity and isolation of the amygdala from other key regions.
133. 1. Neuroscience

     Zona incerta distributes a broad movement signal that modulates behavior

     Sebastian Hormigo, Ji Zhou ... Manuel Castro-Alamancos

     The zona incerta distributes a broad corollary signal of movement occurrence to its projection sites, which regulates but does not drive behavior.
134. 1. Chromosomes and Gene Expression
     2. Neuroscience

     Avoiding false discoveries in single-cell RNA-seq by revisiting the first Alzheimer’s disease dataset

     Alan E Murphy, Nurun Fancy, Nathan Skene

     Reanalysis reveals the impact of quality control and differential analysis methods on the discovery of disease-associated genes on the first Alzheimer's disease single-nucleus RNA-seq dataset.
135. 1. Evolutionary Biology
     2. Genetics and Genomics

     The genomic footprint of social stratification in admixing American populations

     Alex Mas-Sandoval, Sara Mathieson, Matteo Fumagalli

     Social hierarchies resulting from the European colonization of the Americas stratified the population structure, leading to ancestry-related assortative mating and sex bias patterns that can be inferred from the genomes of the populations across the continent.
136. 1. Neuroscience

     Event-related modulation of alpha rhythm explains the auditory P300-evoked response in EEG

     Alina Studenova, Carina Forster ... Vadim Nikulin

     The decrease in alpha rhythm amplitude in the parietal regions after the presentation of a deviant auditory stimulus gives rise to a part of the P300-evoked response.
137. 1. Developmental Biology

     Dynamic compartmentalization of the pro-invasive transcription factor NHR-67 reveals a role for Groucho in regulating a proliferative-invasive cellular switch in C. elegans

     Taylor N Medwig-Kinney, Brian A Kinney ... David Q Matus

     Dynamic compartmentalization of the pro-invasive transcription factor NHR-67 reveals a role for members of the Groucho/TCF repressive complex in maintaining non-invasive cell fate in C. elegans.
138. 1. Neuroscience

     Catecholaminergic neuromodulation and selective attention jointly shape perceptual decision-making

     Stijn A Nuiten, Jan Willem de Gee ... Simon van Gaal

     Pharmacologically elevated catecholamine levels and spatial attention jointly shape perceptual decision-making, revealed by unique, similar, and interactive effects on behavior, drift diffusion modeling parameters, and electrophysiology.