January 2023

Research articles

1. 1. Cell Biology
   2. Genetics and Genomics

   T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation

   Alexandro Landshammer, Adriano Bolondi ... Alexander Meissner

   Discovery of a novel long non-coding RNA overlapping the distal SOX17 enhancer (eSOX17) that plays an essential role in human endoderm development.
2. 1. Stem Cells and Regenerative Medicine

   Intermittent fasting induces rapid hepatocyte proliferation to restore the hepatostat in the mouse liver

   Abby Sarkar, Yinhua Jin ... Roel Nusse

   Hepatocytes proliferate to maintain a constant liver-to-body mass ratio during intermittent fasting.
3. 1. Neuroscience

   Interacting rhythms enhance sensitivity of target detection in a fronto-parietal computational model of visual attention

   Amélie Aussel, Ian C Fiebelkorn ... Benjamin Rafael Pittman-Polletta

   A biophysical cortical circuit model reveals how thalamic inputs mediate complex dynamics in the frontal eye fields and lateral intraparietal area, enabling rhythmic enhancements in visual sensitivity observed behaviorally.
4. 1. Epidemiology and Global Health
   2. Medicine

   The impact of lag time to cancer diagnosis and treatment on clinical outcomes prior to the COVID-19 pandemic: A scoping review of systematic reviews and meta-analyses

   Parker Tope, Eliya Farah ... Eduardo L Franco

   Investigating standard lag times prior to the pandemic and identifying key methodological considerations in lag time research can guide future investigations into the impact of time to care for patients with cancer.
5. 1. Neuroscience

   Olfactory receptor neurons generate multiple response motifs, increasing coding space dimensionality

   Brian Kim, Seth Haney ... Mark A Stopfer

   The odor-elicited responses of olfactory receptor neurons consist of a discrete set of four spike pattern motifs, each with distinct adaptation properties, together amplifying distinctions between similar and temporally complex chemical inputs such as odor plumes.
6. 1. Cell Biology

   Impaired iron recycling from erythrocytes is an early hallmark of aging

   Patryk Slusarczyk, Pratik Kumar Mandal ... Katarzyna Mleczko-Sanecka

   A decline in iron-recycling functions of the splenic red pulp macrophages early during aging is driven by iron loading and involves their damage and a loss in red blood cell clearance capacity.
7. 1. Evolutionary Biology

   Microstructural and crystallographic evolution of palaeognath (Aves) eggshells

   Seung Choi, Mark E Hauber ... David J Varricchio

   The eggshells of palaeognath birds (e.g. ostrich, moa, kiwi, emu) have diverse homology and convergent features, and are useful modern analogues for the evolution of non-avian dinosaur eggshells.
8. 1. Neuroscience
   2. Genetics and Genomics

   Ecdysone acts through cortex glia to regulate sleep in Drosophila

   Yongjun Li, Paula Haynes ... Amita Sehgal

   Effects of ecdysone on sleep are mediated through lipid metabolism in cortex glia.
9. 1. Cell Biology
   2. Neuroscience

   Pericytes control vascular stability and auditory spiral ganglion neuron survival

   Yunpei Zhang, Lingling Neng ... Xiaorui Shi

   Pericytes in the adult ear are critical for vascular stability and spiral ganglion neuron health.
10. 1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells

    Yasunari Seita, Keren Cheng ... Kotaro Sasaki

    In vitro model of germline specification in marmosets established by directed differentiation of induced pluripotent stem cells, which transcriptionally resemble pre-migratory primordial germ cells in vivo.
11. 1. Developmental Biology

    A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus

    Kavitha Chinnaiya, Sarah Burbridge ... Marysia Placzek

    Fate-mapping, combined with gain- and loss-of-function approaches, reveal the role of BMP signalling in the spatio-temporal development of the tuberal hypothalamus, a critically important brain region.
12. 1. Physics of Living Systems

    Geometric control of myosin II orientation during axis elongation

    Matthew F Lefebvre, Nikolas H Claussen ... Sebastian J Streichan

    During Drosophila axis elongation, the orientational pattern of the cytoskeleton is in good approximation stationary, while the genes thought to instruct these patterns are transported with the tissue flow.
13. 1. Medicine
    2. Cancer Biology

    International multicenter study comparing COVID-19 in patients with cancer to patients without cancer: Impact of risk factors and treatment modalities on survivorship

    Issam I Raad, Ray Hachem ... Anne-Marie Chaftari

    Remdesivir is a promising treatment modality to reduce 30 day all-cause mortality.
14. 1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Plasmodium infection disrupts the T follicular helper cell response to heterologous immunization

    Mary F Fontana, Erica Ollmann Saphire, Marion Pepper

    Flow cytometric analysis of antigen-specific B and T cell responses to heterologous immunization reveals intact dendritic cell function, but selective defects in germinal center formation, in mice with concurrent malaria.
15. 1. Cell Biology
    2. Neuroscience

    Growth cone advance requires EB1 as revealed by genomic replacement with a light-sensitive variant

    Alessandro Dema, Rabab Charafeddine ... Torsten Wittmann

    Genome editing to insert a light-sensitive dimerization module directly into the EB1 microtubule plus end adaptor gene in human induced pluripotent stem cells enables local and acute optogenetic EB1 inactivation highlighting microtubule functions in developing neuronal growth cones.
16. 1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    An IS-mediated, RecA-dependent, bet-hedging strategy in Burkholderia thailandensis

    Lillian C Lowrey, Leslie A Kent ... Peggy A Cotter

    Some Burkholderia thailandensis strains have evolved a bet-hedging strategy by acquiring insertion sequences positioned such that RecA-dependent homologous recombination between them results in duplication of intervening sequences, which promotes biofilm formation.
17. 1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    Differential chondrogenic differentiation between iPSC derived from healthy and OA cartilage is associated with changes in epigenetic regulation and metabolic transcriptomic signatures

    Nazir M Khan, Martha Elena Diaz-Hernandez ... Hicham Drissi

    The iPSCs derived from OA cartilage showed memory of disease which affect its chondrogenic potential and regulation at epigenetic and metabolic level may be used to control the regenerative potential of these iPSCs.
18. 1. Structural Biology and Molecular Biophysics

    State-specific morphological deformations of the lipid bilayer explain mechanosensitive gating of MscS ion channels

    Yein Christina Park, Bharat Reddy ... José D Faraldo-Gómez

    A theory is presented that explains why some ion channels tend to open in response to membrane stretching, which posits that channel closing is disfavored by the morphological energetics of the lipid bilayer, and increasingly so under lateral tension.
19. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    An atrial fibrillation-associated regulatory region modulates cardiac Tbx5 levels and arrhythmia susceptibility

    Fernanda M Bosada, Karel van Duijvenboden ... Vincent M Christoffels

    Modest changes in expression of interacting dose-dependent transcription factors Tbx5 and Prrx1, caused by disease-associated common regulatory variants, significantly impact on the cardiac gene regulatory network and disease susceptibility.
20. 1. Cell Biology
    2. Medicine

    Efficacy and safety of metabolic interventions for the treatment of severe COVID-19: in vitro, observational, and non-randomized open-label interventional study

    Avner Ehrlich, Konstantinos Ioannidis ... Yaakov Nahmias

    Human metabolism-focused screens reveal clinically promising interventional strategies and repurposing targets to treat COVID-19.
21. 1. Medicine

    Phenome-wide Mendelian randomisation analysis identifies causal factors for age-related macular degeneration

    Thomas H Julian, Johnathan Cooper-Knock ... Panagiotis I Sergouniotis

    A phenome-wide Mendelian randomisation analysis revealed a causal link between age-related macular degeneration and a number of lipid, complement, immune cell, and serum protein traits, highlighting potential treatment targets.
22. 1. Immunology and Inflammation

    Regulatory T cells suppress the formation of potent KLRK1 and IL-7R expressing effector CD8 T cells by limiting IL-2

    Oksana Tsyklauri, Tereza Chadimova ... Ondrej Stepanek

    Regulatory T cell suppress cytotoxic T cells in autoimmunity and cancer by taking up IL-2.
23. 1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics

    Drug specificity and affinity are encoded in the probability of cryptic pocket opening in myosin motor domains

    Artur Meller, Jeffrey M Lotthammer ... Gregory R Bowman

    A cryptic pocket in the myosin motor domain selectively opens in molecular dynamics simulations, and the probability of pocket opening is predictive of how potently a compound will inhibit a particular myosin motor.
24. 1. Neuroscience

    Internal neural states influence the short-term effect of monocular deprivation in human adults

    Yiya Chen, Yige Gao ... Jiawei Zhou

    Having the eye open under the patch, even though this does not change the exogenous stimulation because the eye is occluded, will result in an enhanced short-term effect for ocular dominance due to the internal neural states.
25. 1. Neuroscience
    2. Physics of Living Systems

    Scale-free behavioral dynamics directly linked with scale-free cortical dynamics

    Sabrina A Jones, Jacob H Barfield ... Woodrow L Shew

    Complex, multi-scale natural behaviors may arise from subpopulations of neurons in cerebral cortex operating near the tipping point of a phase transition, that is, near criticality.
26. 1. Cell Biology
    2. Physics of Living Systems

    Novel analytical tools reveal that local synchronization of cilia coincides with tissue-scale metachronal waves in zebrafish multiciliated epithelia

    Christa Ringers, Stephan Bialonski ... Nathalie Jurisch-Yaksi

    Novel frequency-based analysis and computation modeling of cilia carpets reveals that tissue-scale metachronal coordination of cilia can arise even in the absence of global synchronization.
27. 1. Microbiology and Infectious Disease
    2. Biochemistry and Chemical Biology

    Principles of RNA recruitment to viral ribonucleoprotein condensates in a segmented dsRNA virus

    Sebastian Strauss, Julia Acker ... Ralf Jungmann

    Spatial transcriptomics analysis of rotavirus replication factories reveals principles of viral transcript partitioning and RNA stoichiometry in these granules.
28. 1. Cell Biology
    2. Chromosomes and Gene Expression

    Recruitment of Polo-like kinase couples synapsis to meiotic progression via inactivation of CHK-2

    Liangyu Zhang, Weston T Stauffer ... Abby F Dernburg

    Genetic and cytological analyses reveal a regulatory circuit that controls a key cell cycle transition during meiosis and mediates feedback regulation in response to meiotic defects.
29. 1. Neuroscience

    Signal denoising through topographic modularity of neural circuits

    Barna Zajzon, David Dahmen ... Renato Duarte

    Topographic maps can gradually increase the fidelity of sensory representations and improve signal-to-noise ratio across multiple cortical circuits, a generic architectural feature that depends solely on the modularity of topographic projections and recurrent inhibition.
30. 1. Evolutionary Biology

    Mammalian forelimb evolution is driven by uneven proximal-to-distal morphological diversity

    Priscila S Rothier, Anne-Claire Fabre ... Anthony Herrel

    The diversification of hand bones in mammals was much more dynamic than those of the proximal and intermediate forearm, involving higher morphological diversity, stronger integration, and greater evolutionary lability at distal structures.
31. 1. Structural Biology and Molecular Biophysics

    Activation-pathway transitions in human voltage-gated proton channels revealed by a non-canonical fluorescent amino acid

    Esteban Suárez-Delgado, Maru Orozco-Contreras ... Leon D Islas

    Combined patch-clamp fluorometry with ANAP, a fluorescent non-canonical amino acid, uncovers transitions in the activation pathway of human H_V1 that are modulated by voltage and the pH gradient.
32. 1. Microbiology and Infectious Disease

    GWAS and functional studies suggest a role for altered DNA repair in the evolution of drug resistance in Mycobacterium tuberculosis

    Saba Naz, Kumar Paritosh ... Vinay Kumar Nandicoori

    Genome sequence analysis of Mycobacterium tuberculosis clinical isolates and subsequent validation in laboratory and animal infection models identify previously unrecognized genetic mutations in DNA repair genes that contribute to the development of drug resistance.
33. 1. Neuroscience

    Neurexins in serotonergic neurons regulate neuronal survival, serotonin transmission, and complex mouse behaviors

    Amy Cheung, Kotaro Konno ... Kensuke Futai

    Neurexins are presynaptic adhesion molecules in the serotonin system which regulate neuron survival and serotonin neuromodulation through active zone formation and serotonin release.
34. 1. Cell Biology
    2. Chromosomes and Gene Expression

    LSD1 defines the fiber type-selective responsiveness to environmental stress in skeletal muscle

    Hirotaka Araki, Shinjiro Hino ... Mitsuyoshi Nakao

    Lysine-specific demethylase-1 acts as an epigenetic barrier against glucocorticoid-induced atrophy and exercise-induced hypertrophy in skeletal muscle.
35. 1. Epidemiology and Global Health

    Nationwide participation in FIT-based colorectal cancer screening in Denmark during the COVID-19 pandemic: An observational study

    Tina Bech Olesen, Henry Jensen ... Morten Rasmussen

    The participation in the FIT-based colorectal cancer screening programme and subsequent compliance to colonoscopy after a positive FIT test was only slightly affected during the COVID-19 pandemic in Denmark.
36. 1. Developmental Biology
    2. Evolutionary Biology

    Metamorphosis of memory circuits in Drosophila reveals a strategy for evolving a larval brain

    James W Truman, Jacquelyn Price ... Tzumin Lee

    The mushroom body neuropils of the larval brain have regions that share neurons and functions with the adult and larval-specific regions built with 'doomed' neurons or cells that trans-differentiate for temporary larval function before assuming their adult phenotypes at metamorphosis.
37. 1. Structural Biology and Molecular Biophysics

    The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins

    Alexandria N Miller, Patrick R Houlihan ... David E Clapham

    SARS coronaviruses encode several mysterious proteins such as Orf3a and, in contrast to what has been claimed, Orf3a is not an ion channel but rather disrupts cellular trafficking by binding to the protein sorting complex known as HOPS.
38. 1. Cell Biology

    A concerted mechanism involving ACAT and SREBPs by which oxysterols deplete accessible cholesterol to restrict microbial infection

    David B Heisler, Kristen A Johnson ... Arun Radhakrishnan

    An essential role for ACAT in conferring 25HC-mediated protection against bacterial and viral infection has been uncovered.
39. 1. Cancer Biology

    Oncogenic PKA signaling increases c-MYC protein expression through multiple targetable mechanisms

    Gary KL Chan, Samantha Maisel ... John D Gordan

    Protein kinase A-driven increases in c-MYC protein expression and tumor cell proliferation can be blocked by eIF4A inhibitors, suggesting a potential new treatment option for patients with oncogenic PKA activation.
40. 1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Structures of human dynein in complex with the lissencephaly 1 protein, LIS1

    Janice M Reimer, Morgan E DeSantis ... Andres E Leschziner

    Structures of human cytoplasmic dynein-1 bound to its regulator LIS1 reveal the interfaces involved in forming the complex and provide a context for disease-linked mutations.
41. 1. Computational and Systems Biology
    2. Neuroscience

    A hardware system for real-time decoding of in vivo calcium imaging data

    Zhe Chen, Garrett J Blair ... Hugh T Blair

    DeCalciOn is a low-cost open-source hardware system for real-time in vivo calcium imaging that offers capabilities for online decoding of neural population activity and delivery of short latency closed-loop feedback in freely behaving animals.
42. 1. Neuroscience

    Hierarchical architecture of dopaminergic circuits enables second-order conditioning in Drosophila

    Daichi Yamada, Daniel Bushey ... Yoshinori Aso

    A slow and stable memory unit instructs fast and transient units by activating dopaminergic neurons via an excitatory hub interneuron connecting those units during second-order conditioning in Drosophila.
43. 1. Computational and Systems Biology
    2. Immunology and Inflammation

    The use of non-functional clonotypes as a natural calibrator for quantitative bias correction in adaptive immune receptor repertoire profiling

    Anastasia O Smirnova, Anna M Miroshnichenkova ... Alexander Komkov

    A hallmark property of non-functional clonotypes was used to develop a universal and fully computational method for detection and correction of multiplex PCR-specific quantitative bias in adaptive immune receptor repertoire.
44. 1. Cancer Biology
    2. Microbiology and Infectious Disease

    Beta human papillomavirus 8E6 promotes alternative end joining

    Changkun Hu, Taylor Bugbee ... Nicholas Wallace

    Human papillomavirus type 8 E6 promotes alternative end joining by compromising non-homologous end joining and homologous recombination.
45. 1. Neuroscience

    Selective integration of diverse taste inputs within a single taste modality

    Julia U Deere, Arvin A Sarkissian ... Anita V Devineni

    Bitter-sensing cells across different organs of the fruit fly activate overlapping neural pathways in the brain to regulate a common set of aversive behaviors.
46. 1. Ecology
    2. Microbiology and Infectious Disease

    Bacterial lifestyle switch in response to algal metabolites

    Noa Barak-Gavish, Bareket Dassa ... Assaf Vardi

    Opportunistic bacteria modulate their lifestyle from coexistence to pathogenicity by perceiving the physiological state of their algal host through sensing of algal secreted metabolites.
47. 1. Neuroscience

    Infant brain regional cerebral blood flow increases supporting emergence of the default-mode network

    Qinlin Yu, Minhui Ouyang ... Hao Huang

    Unprecedented 4D spatiotemporal infant regional cerebral blood flow framework and region-specific physiology–function coupling across infancy were elucidated, highlighting strong physiology–function coupling specifically at the default-mode network to meet extraneuronal metabolic demand for network emergence.
48. 1. Epidemiology and Global Health
    2. Medicine

    Antibody levels following vaccination against SARS-CoV-2: associations with post-vaccination infection and risk factors in two UK longitudinal studies

    Nathan J Cheetham, Milla Kibble ... Claire J Steves

    Third SARS-CoV-2 vaccination appears to eliminate disparities in anti-Spike antibodies between those who received Pfizer-BioNTech versus Oxford/AstraZeneca for first and second vaccination, but levels remain lower in certain groups such as those on the UK 'Shielded Patient List'.
49. 1. Microbiology and Infectious Disease

    Initiation of HIV-1 Gag lattice assembly is required for recognition of the viral genome packaging signal

    Xiao Lei, Daniel Gonçalves-Carneiro ... Paul D Bieniasz

    Initiation of the assembly of HIV-1 particles in infected cells is required to form a subviral structure that recognizes the viral RNA genome for packaging.
50. 1. Neuroscience

    Chloride-dependent mechanisms of multimodal sensory discrimination and nociceptive sensitization in Drosophila

    Nathaniel J Himmel, Akira Sakurai ... Daniel N Cox

    Anoctamin/TMEM16 and SLC12 co-transporter genes selectively function in regulating cold nociception via excitatory chloride currents and can promote neuropathic sensitization phenotypes, including behavioral sensitization and neuronal hyperexcitability in Drosophila multimodal sensory neurons.
51. 1. Microbiology and Infectious Disease

    Ribosomal RNA (rRNA) sequences from 33 globally distributed mosquito species for improved metagenomics and species identification

    Cassandra Koh, Lionel Frangeul ... Maria-Carla Saleh

    A score-based read selection strategy enables the assembly of novel full-length ribosomal RNA sequences for mosquitoes, which improves the physical and computational removal of interfering ribosomal RNA reads in RNA-seq and provides another molecular marker for taxonomic and phylogenetic inquiries.
52. 1. Neuroscience

    Soluble amyloid-β precursor peptide does not regulate GABA_B receptor activity

    Pascal Dominic Rem, Vita Sereikaite ... Bernhard Bettler

    Soluble amyloid-β precursor peptide has no functional effects at recombinant and native GABA_B receptors.
53. 1. Immunology and Inflammation

    Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis

    Gerdien Mijnheer, Nila Hendrika Servaas ... Femke van Wijk

    In localized autoimmune disease, there is autoantigen-driven expansion of both effector T cell and Treg clones that are highly persistent and are (re)circulating, and might represent interesting therapeutic targets.
54. 1. Epidemiology and Global Health

    Participation in the nationwide cervical cancer screening programme in Denmark during the COVID-19 pandemic: An observational study

    Tina Bech Olesen, Henry Jensen ... Berit Andersen

    The participation in cervical cancer screening in Denmark was reduced at the start of the pandemic although with longer follow-up time most women resumed screening.
55. 1. Computational and Systems Biology
    2. Immunology and Inflammation

    Structure-based prediction of T cell receptor:peptide-MHC interactions

    Philip Bradley

    The AlphaFold protein structure prediction network can be specialized for T cell receptor docking, leading to improved models of ternary complexes and some ability to discriminate correct from incorrect peptide epitopes.
56. 1. Microbiology and Infectious Disease

    Community composition shapes microbial-specific phenotypes in a cystic fibrosis polymicrobial model system

    Fabrice Jean-Pierre, Thomas H Hampton ... George A O'Toole

    A cystic fibrosis (CF) lung microbiome-informed in vitro polymicrobial system allows for a mechanistic understanding of community-specific tolerance of Pseudomonas aeruginosa to a front-line CF antimicrobial.
57. 1. Cell Biology

    A CRISPR screen in intestinal epithelial cells identifies novel factors for polarity and apical transport

    Katharina MC Klee, Michael W Hess ... Lukas A Huber

    Applying a CRISPR knockout screen in polarized intestinal epithelial cells and subsequent phenotypic analyses identified various genes, of which several were previously not associated with epithelial homeostasis, to be relevant for proper epithelial polarization, apical cargo transport, and endo/lysosomal morphology.
58. 1. Immunology and Inflammation
    2. Physics of Living Systems

    Quantifying changes in the T cell receptor repertoire during thymic development

    Francesco Camaglia, Arie Ryvkin ... Nir Friedman

    Sequence signatures can be used to discriminate between selected and non-selected immune repertoires at different stages only at the collective population level but not at the level of single cells.
59. 1. Epidemiology and Global Health

    A modelling approach to estimate the transmissibility of SARS-CoV-2 during periods of high, low, and zero case incidence

    Nick Golding, David J Price ... Freya M Shearer

    A new statistical model for tracking current and potential rates of disease transmission.
60. 1. Neuroscience

    Multimodal mapping of cell types and projections in the central nucleus of the amygdala

    Yuhan Wang, Sabine Krabbe ... Scott M Sternson

    In the central amygdala, transcriptomic definition of cell types and corresponding spatial transcriptomic analysis reveals major regional differences in molecular organization and relates newly identified molecularly defined cell types to major axon projection targets.
61. 1. Cell Biology
    2. Developmental Biology

    Control of craniofacial development by the collagen receptor, discoidin domain receptor 2

    Fatma F Mohamed, Chunxi Ge ... Renny T Franceschi

    Discoidin domain receptor 2 controls craniofacial morphology by acting in skeletal progenitor cells and chondrocytes to control collagen matrix organization, chondrocyte polarity, and growth.
62. 1. Neuroscience

    A striatal circuit balances learned fear in the presence and absence of sensory cues

    Michael Kintscher, Olexiy Kochubey, Ralf Schneggenburger

    Direct- and indirect pathway neurons of the posterior striatum display divergent in vivo and ex vivo plasticity after fear learning, and differentially modulate defensive behaviors displayed in the presence, and absence of threat-predicting sensory cues.
63. 1. Immunology and Inflammation

    T follicular helper 17 (Tfh17) cells are superior for immunological memory maintenance

    Xin Gao, Kaiming Luo ... Di Yu

    By comparing different subsets in mouse inducible Tfh (iTfh) cells and human circulating Tfh (cTfh) cells after vaccination, Tfh17 cells were found to be better than Tfh1 or Tfh2 cells in maintenance and support immunological memory of humoral immunity.
64. 1. Cancer Biology

    Ras/MAPK signalling intensity defines subclonal fitness in a mouse model of hepatocellular carcinoma

    Anthony Lozano, Francois-Régis Souche ... Damien Grégoire

    Specific tissue environments provide selective pressures that give rise to distinct Ras oncogenic dosage in primary liver tumours and their metastatic-like outgrowths.
65. 1. Computational and Systems Biology
    2. Neuroscience

    Temporal derivative computation in the dorsal raphe network revealed by an experimentally driven augmented integrate-and-fire modeling framework

    Emerson F Harkin, Michael B Lynn ... Jean-Claude Béïque

    A new type of biologically constrained spiking neural network model applied to the dorsal raphe nucleus shows that the output of the serotonin system reflects not just its raw input, but mainly how quickly its input changes over time.
66. 1. Ecology

    Protein feeding mediates sex pheromone biosynthesis in an insect

    Shiyu Gui, Boaz Yuval ... Daifeng Cheng

    Protein feeding stimulates the production of sex pheromones and promotes fly mating in Bactrocera dorsalis.
67. 1. Cell Biology

    Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissue

    Theodore J Zwang, Rachel E Bennett ... Bradley T Hyman

    Conditions for multiplexed antibody labeling in mouse brain tissue translates to effective labeling in human brain tissue when samples are prepared similarly.
68. 1. Biochemistry and Chemical Biology

    Hypoxia truncates and constitutively activates the key cholesterol synthesis enzyme squalene monooxygenase

    Hudson W Coates, Isabelle M Capell-Hattam ... Andrew J Brown

    Hypoxic accumulation of squalene, the substrate of squalene monooxygenase, triggers its proteasomal truncation to a constitutively active variant that preserves downstream sterol synthesis.
69. 1. Cancer Biology

    Selective androgen receptor degrader (SARD) to overcome antiandrogen resistance in castration-resistant prostate cancer

    Meng Wu, Rongyu Zhang ... Jinming Zhou

    The rational drug design combining the bioassay identified a novel selective androgen receptor (AR) degrader for both AR and AR-VRs and illustrated the synergistic importance of AR antagonism and degradation in advanced prostate cancer treatment.
70. 1. Cell Biology
    2. Developmental Biology

    Trisomy 21 induces pericentrosomal crowding delaying primary ciliogenesis and mouse cerebellar development

    Cayla E Jewett, Bailey L McCurdy ... Chad G Pearson

    Trisomy 21, the genetic cause of Down syndrome, produces elevated centrosome protein levels reducing intracellular trafficking to and from the centrosome thereby delaying primary ciliogenesis, ciliary signaling, and mouse cerebellar development.
71. 1. Stem Cells and Regenerative Medicine

    Scleraxis-lineage cells are required for tendon homeostasis and their depletion induces an accelerated extracellular matrix aging phenotype

    Antonion Korcari, Anne EC Nichols ... Alayna E Loiselle

    Age-related tendon degeneration is initiated by loss of tenocytes associated with synthesis of high-turnover rate proteoglycans and glycoproteins.
72. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    STAT3 promotes RNA polymerase III-directed transcription by controlling the miR-106a-5p/TP73 axis

    Cheng Zhang, Shasha Zhao ... Wensheng Deng

    The miR-106a-5p links STAT3 with TP73 to activate Pol III-dependent transcription and cancer cell proliferative activity.
73. 1. Neuroscience

    Position representations of moving objects align with real-time position in the early visual response

    Philippa Anne Johnson, Tessel Blom ... Hinze Hogendoorn

    To accurately represent object position in real time, the human visual system predictively encodes the location of moving objects, compensating for the time required for transmission and processing of information.
74. 1. Neuroscience

    Gasotransmitter modulation of hypoglossal motoneuron activity

    Brigitte M Browe, Ying-Jie Peng ... Alfredo J Garcia III

    Electrophysiological analyses reveal interactions between gasotransmitter enzymes and their respective products that regulate hypoglossal motoneuron excitability and may drive changes in upper airway tone in obstructive sleep apnea and perturb behaviors such as vocalization and swallowing.
75. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    RNA sequence to structure analysis from comprehensive pairwise mutagenesis of multiple self-cleaving ribozymes

    Jessica M Roberts, James D Beck ... Eric J Hayden

    A high-throughput analysis of several self-cleaving ribozymes reveals the effect of every possible mutation, and every possible pair of mutations, and how patterns in the relative activity data can be mapped to canonical and non-canonical structural elements.
76. 1. Medicine

    Bone circuitry and interorgan skeletal crosstalk

    Mone Zaidi, Se-Min Kim ... Tony Yuen

    Intracellular and interorgan skeletal crosstalk highlights integrative skeletal physiology.
77. 1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Future COVID19 surges prediction based on SARS-CoV-2 mutations surveillance

    Fares Z Najar, Evan Linde ... Pratul K Agarwal

    A real-time genomic surveillance approach based on mutation analysis of SARS-CoV-2 proteins has enabled a priori prediction of surge in number of COVID19 infection cases, including those already observed in July and September 2022.
78. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Targeting RNA:protein interactions with an integrative approach leads to the identification of potent YBX1 inhibitors

    Krystel El Hage, Nicolas Babault ... David Pastré

    The development of an integrative computational-experimental approach for identifying small molecules that can disrupt RNA:protein interactions in vitro and in cells led to the identification of several inhibitors of YBX1 in the micromolar range, including a previously approved drug.
79. 1. Computational and Systems Biology

    Transformer-based deep learning for predicting protein properties in the life sciences

    Abel Chandra, Laura Tünnermann ... Regina Gratz

    The recent developments in large-scale machine learning, especially with the recent Transformer models, display much potential for solving computational problems within protein biology and outcompete traditional computational methods in many recent studies and benchmarks.
80. 1. Neuroscience

    Differential processing of decision information in subregions of rodent medial prefrontal cortex

    Geoffrey W Diehl, A David Redish

    A novel method of recording neural activity from the rodent medial prefrontal cortex reveals that the medial prefrontal cortex is composed of functionally distinct subregions that process economic decision information differentially along a dorso-ventral gradient.
81. 1. Cancer Biology
    2. Cell Biology

    ahctf1 and kras mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma

    Kimberly J Morgan, Karen Doggett ... Joan K Heath

    ELYS, a multifunctional protein with critical roles in the cell cycle, is essential for the vigorous proliferation and survival of difficult-to-treat cancers carrying mutations in the KRAS oncogene, making it a compelling target for novel drug treatments.
82. 1. Developmental Biology
    2. Neuroscience

    Dermal appendage-dependent patterning of zebrafish atoh1a+ Merkel cells

    Tanya L Brown, Emma C Horton ... Jeffrey P Rasmussen

    Molecular, cellular, and developmental characterization of zebrafish Merkel cells establishes a new model for the study of mechanosensory epidermal cells.
83. 1. Immunology and Inflammation
    2. Medicine

    FGF21 protects against hepatic lipotoxicity and macrophage activation to attenuate fibrogenesis in nonalcoholic steatohepatitis

    Cong Liu, Milena Schönke ... Patrick CN Rensen

    Fibroblast growth factor 21 blocks hepatic lipid influx and accumulation, prevents Kupffer cell activation, and inhibits the formation of hepatic lipid- and scar-associated macrophages, thereby likely inhibiting fibrogenesis in nonalcoholic steatohepatitis in humanized APOE*3-Leiden.CETP mice.
84. 1. Evolutionary Biology
    2. Genetics and Genomics

    Relating pathogenic loss-of-function mutations in humans to their evolutionary fitness costs

    Ipsita Agarwal, Zachary L Fuller ... Molly Przeworski

    Loss-of-function mutations in human genes are an important class of disease causing variation, and estimates of their effects on evolutionary fitness can be used to evaluate their pathogenicity.
85. 1. Structural Biology and Molecular Biophysics

    A mechanism of uncompetitive inhibition of the serotonin transporter

    Shreyas Bhat, Ali El-Kasaby ... Walter Sandtner

    Biochemical and electrophysiological approaches show that the amphetamine derivative ECSI#6 is an uncompetitive inhibitor of the serotonin transporter and thus the first ever characterized ligand of a solute carrier of this type.
86. 1. Cell Biology
    2. Immunology and Inflammation

    Spatially resolved transcriptomics reveals pro-inflammatory fibroblast involved in lymphocyte recruitment through CXCL8 and CXCL10

    Ana J Caetano, Yushi Redhead ... Paul T Sharpe

    Integrated single-cell and spatial genomics provides the first molecular map of the human oral mucosa and reveals a novel pro-inflammatory fibroblast involved in disease progression.
87. 1. Physics of Living Systems

    Active morphogenesis of patterned epithelial shells

    Diana Khoromskaia, Guillaume Salbreux

    An epithelium modelled as an active, nematic surface can display flattening, budding, and tubulation morphogenetic events, which are observed during development in biological organisms.
88. 1. Developmental Biology
    2. Neuroscience

    Inter-organ Wingless/Ror/Akt signaling regulates nutrient-dependent hyperarborization of somatosensory neurons

    Yasutetsu Kanaoka, Koun Onodera ... Yukako Hattori

    A muscle-derived Wnt signal promotes dendritic branching of somatosensory neurons in response to a concurrent deficiency in vitamins, minerals, and cholesterol.
89. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Mechanism of Ca²⁺ transport by ferroportin

    Jiemin Shen, Azaan Saalim Wilbon ... Yaping Pan

    Cryo-EM structure and functional analyses show that human iron exporter ferroportin (Fpn) can bind and transport calcium ions and the transport activity is reduced when the concentration of ferrous ions is high.
90. 1. Medicine
    2. Microbiology and Infectious Disease

    Sex and prior exposure jointly shape innate immune responses to a live herpesvirus vaccine

    Foo Cheung, Richard Apps ... Jeffrey I Cohen

    In a study of a herpes simplex virus (HSV) vaccine, the combination of sex and prior exposure to the virus resulted in HSV naive women mounting a prominent type I interferon response associated with reduced neutralizing titers to HSV.
91. 1. Cell Biology
    2. Neuroscience

    Gain-of-function variants in the ion channel gene TRPM3 underlie a spectrum of neurodevelopmental disorders

    Lydie Burglen, Evelien Van Hoeymissen ... Joris Vriens

    Newly identified gain-of-function variants indicate TRPM3 as the cause of a spectrum of autosomal dominant neurodevelopmental disorders with frequent cerebellar involvement in humans.
92. 1. Cell Biology
    2. Developmental Biology

    Ephrin-B1 regulates the adult diastolic function through a late postnatal maturation of cardiomyocyte surface crests

    Clement Karsenty, Celine Guilbeau-Frugier ... Celine Galés

    Eprhin B1 in the cardiomyocyte regulates the maturation of the adult surface crest architecture and of the diastolic function during a late postnatal stage in rodents.
93. 1. Neuroscience

    GluA3 subunits are required for appropriate assembly of AMPAR GluA2 and GluA4 subunits on cochlear afferent synapses and for presynaptic ribbon modiolar–pillar morphology

    Mark A Rutherford, Atri Bhattacharyya ... Maria Eulalia Rubio

    Ultrastructural and imaging studies of cochlear ribbon synapses demonstrate cis- and trans-synaptic molecular and structural effects in the absence of the AMPA-type glutamate receptor subunit GluA3 during mouse development in ambient sound levels.
94. 1. Neuroscience

    Neural assemblies uncovered by generative modeling explain whole-brain activity statistics and reflect structural connectivity

    Thijs L van der Plas, Jérôme Tubiana ... Georges Debrégeas

    A data-driven network model offers an interpretable and physiologically sound description of the whole-brain spontaneous neural activity of zebrafish larvae.
95. 1. Developmental Biology

    Nanobodies combined with DNA-PAINT super-resolution reveal a staggered titin nanoarchitecture in flight muscles

    Florian Schueder, Pierre Mangeol ... Frank Schnorrer

    The two Drosophila titin homologs Sallimus and Projectin display a precise tandem arrangement in the sarcomere that may explain how sarcomere length is regulated outside of vertebrates.
96. 1. Physics of Living Systems

    Dynamics of immune memory and learning in bacterial communities

    Madeleine Bonsma-Fisher, Sidhartha Goyal

    Bacterial CRISPR immunity tracks phage mutations, creating immune diversity in bacterial populations that parallels phage genetic diversity and patterns of phage evolution that are determined by the type and degree of immune cross-reactivity in the CRISPR system.
97. 1. Neuroscience

    Individual behavioral trajectories shape whole-brain connectivity in mice

    Jadna Bogado Lopes, Anna N Senko ... Gerd Kempermann

    When genes and environment are controlled, mice still develop stable differences in behavior, which are associated with large-scale differences in brain structure and connectivity.
98. 1. Medicine

    Dysregulation of the PRUNE2/PCA3 genetic axis in human prostate cancer: from experimental discovery to validation in two independent patient cohorts

    Richard C Lauer, Marc Barry ... Wadih Arap

    PRUNE2/PCA3 axis dysregulation is an early, rather than late, event in prostate cancer tumorigenesis.
99. 1. Developmental Biology

    A nanobody toolbox to investigate localisation and dynamics of Drosophila titins and other key sarcomeric proteins

    Vincent Loreau, Renate Rees ... Dirk Görlich

    A collection of 22 nanobodies against 11 different Drosophila sarcomeric protein epitopes was generated, validated in stainings and for some transgenic fly lines were generated to monitor the dynamics of the sarcomeric proteins in muscles in vivo.
100. 1. Developmental Biology

     Renal interstitial cells promote nephron regeneration by secreting prostaglandin E2

     Xiaoliang Liu, Ting Yu ... Chi Liu

     During nephron regeneration, renal interstitial cells promote the proliferation of renal progenitor cells by secreting PGE2, thus forming the microenvironment of renal progenitor cells.
101. 1. Chromosomes and Gene Expression
     2. Immunology and Inflammation

     Maternal obesity blunts antimicrobial responses in fetal monocytes

     Suhas Sureshchandra, Brianna M Doratt ... Ilhem Messaoudi

     Maternal obesity and high-fat diet present metabolic, signaling, and epigenetic impediments to pathogen recognition in fetal innate immune cells that result in a state of immune paralysis during gestation and at birth.
102. 1. Neuroscience

     Inhibition is a prevalent mode of activity in the neocortex around awake hippocampal ripples in mice

     Javad Karimi Abadchi, Zahra Rezaei ... Majid H Mohajerani

     Neocortical activity is drastically different around awake than sleep hippocampal ripples.
103. 1. Immunology and Inflammation
     2. Microbiology and Infectious Disease

     A hierarchy of cell death pathways confers layered resistance to shigellosis in mice

     Justin L Roncaioli, Janet Peace Babirye ... Russell E Vance

     Genetic analyses using a new oral infection mouse model demonstrate that cell death pathways protect against the gastrointestinal bacterial pathogen Shigella flexneri.
104. 1. Genetics and Genomics
     2. Immunology and Inflammation

     Effects of an IgE receptor polymorphism acting on immunity, susceptibility to infection, and reproduction in a wild rodent

     Klara M Wanelik, Mike Begon ... Steve Paterson

     Genetic variation can have multiple effects on different phenotypic traits in organisms living in the natural environment.
105. 1. Neuroscience

     Exploring therapeutic strategies for infantile neuronal axonal dystrophy (INAD/PARK14)

     Guang Lin, Burak Tepe ... Hugo J Bellen

     Endolysosmal trafficking defects, expansion of lysosomes, elevation of ceramides, and disruption of mitochondria are root causes of INAD/PARK14.
106. 1. Developmental Biology

     MCT1-dependent energetic failure and neuroinflammation underlie optic nerve degeneration in Wolfram syndrome mice

     Greta Rossi, Gabriele Ordazzo ... Vania Broccoli

     The endoplasmic reticulum (ER)-resident protein wolframin regulates MCT1 protein levels in retinal glial cells controlling the shuttling of energy metabolites to both somata and axons of retinal ganglion cells and, thus, supporting their fitness and survival.
107. 1. Developmental Biology
     2. Plant Biology

     The circadian clock controls temporal and spatial patterns of floral development in sunflower

     Carine M Marshall, Veronica L Thompson ... Stacey L Harmer

     The circadian clock acts with light response pathways to tightly synchronize daily rhythms in maturation of hundreds of florets in sunflower, promoting timely visits by pollinators and creating ring-like patterns on developing heads.
108. 1. Neuroscience

     Histological E-data Registration in rodent Brain Spaces

     Jingyi Guo Fuglstad, Pearl Saldanha ... Jonathan R Whitlock

     HERBS is a catch-all anatomical registration toolkit allowing users to plan surgical coordinates in advance, or visualize anatomical data post hoc in 2D or 3D brain volumes for rats, mice, or any species that has a compatible atlas.
109. 1. Cell Biology

     Testing the ion-current model for flagellar length sensing and IFT regulation

     Hiroaki Ishikawa, Jeremy Moore ... Wallace F Marshall

     A model for flagellar length control based on calcium influx is rejected based on quantitative measurements in Chlamydomonas cells.
110. 1. Neuroscience

     Sensorimotor feedback loops are selectively sensitive to reward

     Olivier Codol, Mehrdad Kashefi ... Paul L Gribble

     Motor performance benefits from rewarding contexts via a non-uniform reduction of response latencies and increase in feedback gains across the feedback loops involved in upper limb motor control.
111. 1. Neuroscience

     Human and macaque pairs employ different coordination strategies in a transparent decision game

     Sebastian Moeller, Anton M Unakafov ... Igor Kagan

     Using novel approach to study real-time social interactions shows that mutual action visibility facilitates static and dynamic coordination in both species, but whereas humans employ dynamic turn-taking to equalize rewards, macaques, after training with a human, compete between themselves.
112. 1. Structural Biology and Molecular Biophysics

     Inhibition of the proton-activated chloride channel PAC by PIP₂

     Ljubica Mihaljević, Zheng Ruan ... Zhaozhu Qiu

     PIP₂ inhibits the proton-activated chloride (PAC) channel by selectively stabilizing its desensitized state.
113. 1. Cell Biology
     2. Immunology and Inflammation

     Transiently heritable fates and quorum sensing drive early IFN-I response dynamics

     Laura C Van Eyndhoven, Vincent PG Verberne ... Jurjen Tel

     Early IFN-I response dynamics are initiated in only fractions of cells, of which their epigenetic profile is transiently heritable and subject to the effects of quorum sensing.
114. 1. Genetics and Genomics
     2. Neuroscience

     MicroRNA-eQTLs in the developing human neocortex link miR-4707-3p expression to brain size

     Michael J Lafferty, Nil Aygün ... Jason L Stein

     Inter-individual differences in miRNA expression during fetal cortical development informs genetic mechanisms for complex brain phenotypes.
115. 1. Microbiology and Infectious Disease

     The antidepressant sertraline provides a novel host directed therapy module for augmenting TB therapy

     Deepthi Shankaran, Anjali Singh ... Vivek Rao

     Sertraline, a widely used antidepressant, enhances the potency of the standard antimycobacterial therapy in preclinical models of TB infection.
116. 1. Biochemistry and Chemical Biology
     2. Computational and Systems Biology

     A novel fold for acyltransferase-3 (AT3) proteins provides a framework for transmembrane acyl-group transfer

     Kahlan E Newman, Sarah N Tindall ... Marjan W Van Der Woude

     The modelled structure of a membrane protein supports the hypothesis that it has a new fold with a channel that allows a chemical group to cross the membrane to decorate surface structures.
117. 1. Computational and Systems Biology
     2. Genetics and Genomics

     Uncovering perturbations in human hematopoiesis associated with healthy aging and myeloid malignancies at single-cell resolution

     Marina Ainciburu, Teresa Ezponda ... Felipe Prosper

     Single-cell transcriptomics reveals altered pathways, gene expression dynamics, and activation of transcriptional programs in human early hematopoiesis during healthy aging and myelodysplastic syndromes.
118. 1. Cancer Biology

     EHD2 overexpression promotes tumorigenesis and metastasis in triple-negative breast cancer by regulating store-operated calcium entry

     Haitao Luan, Timothy A Bielecki ... Hamid Band

     Identification of a pro-tumorigenic and pro-metastatic role of EHD2 protein in triple-negative breast cancer (TNBC) through the store-operated calcium entry (SOCE) pathway provides a rationale for potential targeting of EHD2-overexpressing TNBC with SOCE inhibitors.
119. 1. Evolutionary Biology
     2. Structural Biology and Molecular Biophysics

     Hierarchical sequence-affinity landscapes shape the evolution of breadth in an anti-influenza receptor binding site antibody

     Angela M Phillips, Daniel P Maurer ... Michael M Desai

     An anti-influenza receptor binding site antibody acquires breadth through hierarchical sets of epistatic mutations distributed across the light and heavy chains, demonstrating how mutations can interact to shape the evolution of antibody breadth in various antigen exposure regimens.
120. 1. Cancer Biology
     2. Immunology and Inflammation

     Recombinant single-cycle influenza virus with exchangeable pseudotypes allows repeated immunization to augment anti-tumour immunity with immune checkpoint inhibitors

     Matheswaran Kandasamy, Uzi Gileadi ... Vincenzo Cerundolo

     Immunization with NY-ESO-1 S-FLU virus elicits a robust NY-ESO-1-specific CTL response and suppresses the NY-ESO-1-expressing tumour development and spontaneous metastasis.
121. 1. Cell Biology
     2. Chromosomes and Gene Expression

     Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa

     Anita Szőke, Orsolya Sárkány ... Krisztina Káldi

     Molecular timekeeping in Neurospora crassa is robust even under severe limitation of carbon sources and the efficient adaptation to changing nutrient availability is dependent on the positive component of the circadian clock.
122. 1. Cell Biology
     2. Neuroscience

     Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers

     Shenjie Wu, Nancy C Hernandez Villegas ... Randy Schekman

     A pathway of unconventional secretion for alpha-synuclein, a protein which may spread in the brain as part of the pathology of Parkinson’s disease.
123. 1. Evolutionary Biology
     2. Genetics and Genomics

     Balancing selection on genomic deletion polymorphisms in humans

     Alber Aqil, Leo Speidel ... Omer Gokcumen

     The effect of balancing selection on the human genome is greater than previously appreciated, especially affecting large deletion variations.
124. 1. Neuroscience

     Targeted sensors for glutamatergic neurotransmission

     Yuchen Hao, Estelle Toulmé ... Andrew JR Plested

     Targeting a genetically-encoded glutamate sensor to postsynaptic sites demonstrates the feasibility of simultaneous optical quantal analysis of many inputs to a neuron in wide-field fluorescence microscopy.
125. 1. Genetics and Genomics
     2. Microbiology and Infectious Disease

     Interdependent progression of bidirectional sister replisomes in E. coli

     Po Jui Chen, Anna B McMullin ... David Bates

     Physical association between sister replisomes early in the E. coli replication phase promotes rapid fork progression and inhibits fork stalling.
126. 1. Structural Biology and Molecular Biophysics

     Cryo-EM structures of mitochondrial respiratory complex I from Drosophila melanogaster

     Ahmed-Noor A Agip, Injae Chung ... Judy Hirst

     Structures of respiratory complex I from the insect and metazoan model system Drosophila melanogaster reveal its close relationships with the mammalian complex and provide a foundation for new approaches to disentangle mechanisms of complex I catalysis and regulation.
127. 1. Neuroscience

     Temporal context and latent state inference in the hippocampal splitter signal

     Éléonore Duvelle, Roddy M Grieves, Matthijs AA van der Meer

     A synthesis of 20+ years of experimental studies on 'splitter cells' in the hippocampus reveals that signature properties of (1) temporal context models and (2) latent state inference are both needed to account for the data.
128. 1. Medicine
     2. Microbiology and Infectious Disease

     Efficacy of ultra-short, response-guided sofosbuvir and daclatasvir therapy for hepatitis C in a single-arm mechanistic pilot study

     Barnaby Flower, Le Manh Hung ... Graham S Cooke

     Shortened hepatitis C therapy, with retreatment if needed, can reduce antiviral drug use in patients with mild liver disease, but day 2 viral load is not an adequate predictor of outcome.
129. 1. Cancer Biology

     EZH2/hSULF1 axis mediates receptor tyrosine kinase signaling to shape cartilage tumor progression

     Zong-Shin Lin, Chiao-Chen Chung ... Ya-Huey Chen

     EZH2/cMET may serve as biomarkers to stratify chondrosarcoma patients for cMET inhibitor treatment.
130. 1. Cell Biology
     2. Developmental Biology

     Kindlin-2 inhibits TNF/NF-κB-Caspase 8 pathway in hepatocytes to maintain liver development and function

     Huanqing Gao, Yiming Zhong ... Guozhi Xiao

     Biochemical and genetic approaches reveal how the focal adhesion protein kindlin-2 controls the liver development and homeostasis by reducing inflammation.
131. 1. Epidemiology and Global Health
     2. Microbiology and Infectious Disease

     Derivation and external validation of clinical prediction rules identifying children at risk of linear growth faltering

     Sharia M Ahmed, Ben J Brintz ... Daniel T Leung

     Clinical prediction rules could help identify children at risk of slowed growth after an episode of diarrheal illness, and these rules may be generalizable to all children, regardless of diarrhea status.
132. 1. Neuroscience

     Mechanotransduction events at the physiological site of touch detection

     Luke H Ziolkowski, Elena O Gracheva, Sviatoslav N Bagriantsev

     A novel experimental approach reveals how mechanical force acting on the skin is converted into electrical signaling in sensory neurons to evoke the sensation of touch.
133. 1. Neuroscience

     Spatial frequency representation in V2 and V4 of macaque monkey

     Ying Zhang, Kenneth E Schriver ... Anna Wang Roe

     V2 and V4 contain orthogonal maps of orientation and spatial frequency, which indicates a fundamental principle of functional mapping across the cortical surface that ensures and optimizes the complete representation of all combinations across two coding dimensions.
134. 1. Medicine
     2. Neuroscience

     Multimodal brain age estimates relate to Alzheimer disease biomarkers and cognition in early stages: a cross-sectional observational study

     Peter R Millar, Brian A Gordon ... Beau M Ances

     Machine learning-based models of brain age using distinct MRI modalities (structural MRI and functional connectivity) capture complementary signals related to healthy age differences and capture unique patterns related to Alzheimer disease, including stages of disease progression, biomarkers, and cognition.
135. 1. Chromosomes and Gene Expression
     2. Developmental Biology

     Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8

     Nan Wang, Jing He ... Kehkooi Kee

     The lncRNA landscapes in human germ cells are comprehensively profiled and the functional role of an lncRNA LNC1845 in regulating gene expression of a neighboring coding gene LHX8 is elucidated.
136. 1. Cancer Biology

     Dalpiciclib partially abrogates ER signaling activation induced by pyrotinib in HER2⁺HR⁺ breast cancer

     Jiawen Bu, Yixiao Zhang ... Caigang Liu

     Dalpiciclib could partially abrogate ER nuclear transportation induced by pyrotinib in HER2+HR+ breast cancer and CALML5 could serve as a potential risk factor in the treatment of HER2+HR+ breast cancer.
137. 1. Biochemistry and Chemical Biology
     2. Cell Biology

     Disrupting the ciliary gradient of active Arl3 affects rod photoreceptor nuclear migration

     Amanda M Travis, Samiya Manocha ... Jillian N Pearring

     Dominant mutations in Arl3, linked to inherited retinal dystrophy, disrupt the active Arl3-GTP ciliary gradient and cause a defect in rod photoreceptor nuclear migration that can be rescued by elevating ciliary Arl3 activity or reducing aberrant non-ciliary Arl3 activity.
138. 1. Biochemistry and Chemical Biology
     2. Structural Biology and Molecular Biophysics

     Improved ANAP incorporation and VCF analysis reveal details of P2X7 current facilitation and a limited conformational interplay between ATP binding and the intracellular ballast domain

     Anna Durner, Ellis Durner, Annette Nicke

     Comparision of current and fluorescent kinetics from P2X7 receptor mutants labeled with the fluorescent amino acid ANAP suggests that the characteristic P2X7 current facilitation involves changes in channel gating rather than interactions or conformational changes of its intracellular ballast domain.
139. 1. Developmental Biology
     2. Stem Cells and Regenerative Medicine

     Germline/soma distinction in Drosophila embryos requires regulators of zygotic genome activation

     Megan M Colonnetta, Paul Schedl, Girish Deshpande

     Components of zygotic genome activation, Zelda and CLAMP, contribute to the proper specification of primordial germ cells in Drosophila embryos.
140. 1. Cell Biology

     Metformin protects trabecular meshwork against oxidative injury via activating integrin/ROCK signals

     Lijuan Xu, Xinyao Zhang ... Yuanbo Liang

     Metformin, which promotes the recovery of damaged trabecular meshwork, is a potential intraocular pressure-lowering drug.
141. 1. Developmental Biology
     2. Neuroscience

     Single-cell transcriptomic profiling of the zebrafish inner ear reveals molecularly distinct hair cell and supporting cell subtypes

     Tuo Shi, Marielle O Beaulieu ... David W Raible

     Single-cell transcriptomic analysis defines hair cell and supporting cell types in the zebrafish inner ear, and reveals homologies with cells in the mammalian ear.
142. 1. Biochemistry and Chemical Biology

     Dynamic metabolome profiling uncovers potential TOR signaling genes

     Stella Reichling, Peter F Doubleday ... Duncan Holbrook-Smith

     High throughput metabolome profiling of yeast cells that are dynamically perturbed with the drug rapamycin can be used to implicate new genes in the key cellular process of TOR signaling, including the gene of unknown function CFF1.
143. 1. Cell Biology
     2. Stem Cells and Regenerative Medicine

     Mir155 regulates osteogenesis and bone mass phenotype via targeting S1pr1 gene

     Zhichao Zheng, Lihong Wu ... Janak L Pathak

     Mir155 showed a catabolic effect on osteogenesis and bone mass phenotype via interaction with the S1pr1 gene, suggesting inhibition of Mir155 as a potential approach to bone regeneration and bone defect healing.
144. 1. Developmental Biology
     2. Neuroscience

     Molecular characterization of cell types in the squid Loligo vulgaris

     Jules Duruz, Marta Sprecher ... Simon G Sprecher

     Single-cell transcriptomics correlated with in situ gene expression analysis provide an overview of the diversity and molecular features of cell types in the squid Loligo vulgaris.
145. 1. Immunology and Inflammation

     The meningeal transcriptional response to traumatic brain injury and aging

     Ashley C Bolte, Daniel A Shapiro ... John R Lukens

     Transcriptional analyses provide an atlas of how traumatic brain injury and aging shape meningeal cell composition and gene expression.
146. 1. Neuroscience

     Antisense oligonucleotide therapy rescues disturbed brain rhythms and sleep in juvenile and adult mouse models of Angelman syndrome

     Dongwon Lee, Wu Chen ... Mingshan Xue

     Reactivation of paternal Ube3a by an ASO therapy in juvenile and adult mouse models of Angelman syndrome reverses the impairments of EEG power spectrum and sleep pattern, suggesting that these two core disease features may be improved by such therapies.