April 2018

Research articles

1. 1. Cell Biology

   Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates

   Guadalupe Sepulveda, Mark Antkowiak ... Li-En Jao

   During centrosome maturation, pericentrin is delivered to the centrosome co-translationally by a microtubule- and dynein-dependent process, as pericentrin mRNA is undergoing active translation near the centrosome.
2. 1. Cell Biology
   2. Chromosomes and Gene Expression

   Reduced expression of C/EBPβ-LIP extends health and lifespan in mice

   Christine Müller, Laura M Zidek ... Cornelis F Calkhoven

   Reduced expression of a single transcription factor is sufficient to improve fitness and health at old age and increases the lifespan in female mice.
3. 1. Structural Biology and Molecular Biophysics

   All-atom molecular dynamics of the HBV capsid reveals insights into biological function and cryo-EM resolution limits

   Jodi A Hadden, Juan R Perilla ... Klaus Schulten

   All-atom molecular dynamics of the HBV capsid supports a role for structural asymmetry in biological function, reveals the potential for triangular pores to mediate cellular signaling, and indicates that capsid flexibility may limit resolution attainable by cryo-EM.
4. 1. Structural Biology and Molecular Biophysics

   A label-free approach to detect ligand binding to cell surface proteins in real time

   Verena Burtscher, Matej Hotka ... Walter Sandtner

   Binding of a charged small molecule to a membrane protein changes the amplitude of the apparent membrane capacitance by changing the charge density at the surfaces of the membrane.
5. 1. Neuroscience

   VEGF signaling regulates the fate of obstructed capillaries in mouse cortex

   Patrick Reeson, Kevin Choi, Craig E Brown

   Mapping the fate of clogged brain capillaries reveals that VEGF signaling plays a critical role in dictating whether capillaries regain blood flow and persist or are pruned from the vascular tree.
6. 1. Neuroscience

   Large-scale two-photon imaging revealed super-sparse population codes in the V1 superficial layer of awake monkeys

   Shiming Tang, Yimeng Zhang ... Tai Sing Lee

   Two-photon imaging reveals super-sparse responses to natural images in primate V1, which carry sufficient information for discrimination of the input natural images with high accuracy.
7. 1. Computational and Systems Biology
   2. Plant Biology

   Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression

   Peter D Gould, Mirela Domijan ... James CW Locke

   Single cell analysis reveals how period differences between cells and cell-to-cell coupling generates the spatial structure of the plant circadian clock.
8. 1. Cell Biology
   2. Chromosomes and Gene Expression

   Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks

   Bryan A Leland, Angela C Chen ... Megan C King

   A single-cell assay reveals that the genetic rewiring that underlies PARP inhibitor resistance drives altered DNA double-strand break end resection pathway choice.
9. 1. Evolutionary Biology

   Microbial eukaryotes have adapted to hypoxia by horizontal acquisitions of a gene involved in rhodoquinone biosynthesis

   Courtney W Stairs, Laura Eme ... Andrew J Roger

   A number of microbial eukaryotes have adapted to low oxygen conditions by acquiring a gene that allows their mitochondrial electron transport chain to continue to function when oxygen is scarce.
10. 1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Two complement receptor one alleles have opposing associations with cerebral malaria and interact with α⁺thalassaemia

    D Herbert Opi, Olivia Swann ... J Alexandra Rowe

    Analysis of a large Kenyan dataset may resolve previously conflicting studies by identifying polymorphisms which interact to modify the risk of cerebral malaria.
11. 1. Neuroscience

    Acute intermittent hypoxia enhances corticospinal synaptic plasticity in humans

    Lasse Christiansen, MA Urbin ... Monica A Perez

    Acute intermittent hypoxia is a noninvasive approach that enhances corticospinal function in humans, likely through alterations in corticospinal-motoneuronal synaptic transmission.
12. 1. Computational and Systems Biology
    2. Neuroscience

    Antagonism in olfactory receptor neurons and its implications for the perception of odor mixtures

    Gautam Reddy, Joseph D Zak ... Venkatesh N Murthy

    Computational and theoretical analyses offer novel and unexpected insight into how complex, naturally occurring odor mixtures are parsed and normalized at the very first stage of olfaction.
13. 1. Cancer Biology

    Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation

    Veronique Nogueira, Krushna C Patra, Nissim Hay

    Exploiting the metabolic consequences of Akt activation in prostate cancer for therapeutic interventions that circumvent Akt inhibition therapy.
14. 1. Developmental Biology

    Regulation of the Drosophila ID protein Extra macrochaetae by proneural dimerization partners

    Ke Li, Nicholas E Baker

    Developmental genetic and cell culture studies indicate that the Inhibitor of DNA-binding protein Extra macrochaetae (Emc), previously thought to determine where bHLH proteins can act, is itself regulated by those bHLH dimerization partners at the level of protein stability.
15. 1. Plant Biology

    Landscape genomic prediction for restoration of a Eucalyptus foundation species under climate change

    Megan Ann Supple, Jason G Bragg ... Justin O Borevitz

    Empirical patterns of genomic and phenotypic variation across the landscape are used to develop a predictive model that will help build resilient ecological communities.
16. 1. Plant Biology

    A tension-adhesion feedback loop in plant epidermis

    Stéphane Verger, Yuchen Long ... Olivier Hamant

    Tensile stress patterns in tissues are revealed by cell-cell adhesion defects; in turn, cell responses to supracellular tension require cell-cell adhesion.
17. 1. Neuroscience

    Optical detection of three modes of endocytosis at hippocampal synapses

    Natali L Chanaday, Ege T Kavalali

    Single synaptic vesicle imaging shows that kinetically distinct endocytic pathways are differentially regulated by calcium and temperature, and influence the fidelity of synaptic vesicle protein retrieval.
18. 1. Ecology
    2. Epidemiology and Global Health

    Ecological multiplex interactions determine the role of species for parasite spread amplification

    Massimo Stella, Sanja Selakovic ... Cecilia S Andreazzi

    Predators may amplify multiple-host parasite spreading in systems with multiple transmission routes.
19. 1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    Stochastic processes constrain the within and between host evolution of influenza virus

    John T McCrone, Robert J Woods ... Adam S Lauring

    An analysis of influenza viruses from naturally infected people suggests a tight transmission bottleneck and little positive selection within hosts.
20. 1. Neuroscience

    Embryonic and postnatal neurogenesis produce functionally distinct subclasses of dopaminergic neuron

    Elisa Galliano, Eleonora Franzoni ... Matthew S Grubb

    In the mouse olfactory bulb, different types of local dopaminergic interneuron are produced before and after birth.
21. 1. Neuroscience

    Oxytocin functions as a spatiotemporal filter for excitatory synaptic inputs to VTA dopamine neurons

    Lei Xiao, Michael F Priest, Yevgenia Kozorovitskiy

    Oxytocin, a peptide linked to the processing of socially meaningful stimuli, modulates excitatory synaptic transmission in dopaminergic neurons of ventral tegmental area via retrograde endocannabinoid signaling, acting as a pathway-specific temporal filter for synaptic inputs.
22. 1. Cell Biology

    Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy

    Gian-Luca McLelland, Thomas Goiran ... Edward A Fon

    A crucial step during the mitophagy cascade involves the disassembly of connections between mitochondria and the endoplasmic reticulum via the retrotranslocation of Mfn2 tethering complexes by the Parkinson's disease genes PARKIN and PINK1, as well as the ATPase VCP/p97.
23. 1. Developmental Biology

    HIF-2α is essential for carotid body development and function

    David Macias, Andrew S Cowburn ... Randall S Johnson

    Genetic and physiological analyses demonstrate the essential role of the transcription factor HIF-2alpha in the sympathetic nervous system control of the ventilatory response to hypoxia.
24. 1. Developmental Biology
    2. Neuroscience

    Directional selectivity of afferent neurons in zebrafish neuromasts is regulated by Emx2 in presynaptic hair cells

    Young Rae Ji, Sunita Warrier ... Katie S Kindt

    Emx2 mediates the directional selectivity of neuromasts by regulating hair bundle orientation in hair cells, and by selecting afferent neuronal targets.
25. 1. Neuroscience

    Cellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics

    Vincent Croset, Christoph D Treiber, Scott Waddell

    Sequencing mRNA from thousands of single cells from the Drosophila brain highlights the extent of cellular diversity and reveals co-expression of specific neuropeptides with particular fast-acting neurotransmitters and monoamines.
26. 1. Neuroscience

    Biophysics of object segmentation in a collision-detecting neuron

    Richard Burkett Dewell, Fabrizio Gabbiani

    Active dendritic processing enables an individual neuron to discriminate the spatial pattern of synaptic inputs, increasing neural and behavioral selectivity for escaping an impending threat.
27. 1. Structural Biology and Molecular Biophysics

    Intracellular antibody signalling is regulated by phosphorylation of the Fc receptor TRIM21

    Claire Dickson, Adam J Fletcher ... Leo C James

    Phosphorylation deactivates TRIM21 B Box autoinhibition to licence proinflammatory signalling by cytosolic antibodies.
28. 1. Neuroscience

    Rat anterior cingulate cortex recalls features of remote reward locations after disfavoured reinforcements

    Ali Mashhoori, Saeedeh Hashemnia ... Aaron J Gruber

    The anterior cingulate cortex intermixes a precise spatial map with a cognitive map of the task, and spontaneously recalls multimodal information about unrealized choice outcomes during pauses in behavior after reinforcements.
29. 1. Structural Biology and Molecular Biophysics

    Hexameric and pentameric complexes of the ExbBD energizer in the Ton system

    Saori Maki-Yonekura, Rei Matsuoka ... Koji Yonekura

    Crystallography and single particle cryo-EM reveal hexameric and pentameric structures of the ExbBD energizer in the Ton system and oligomeric transition, which provides a unique working mechanism and a new vision of dynamic membrane biology.
30. 1. Plant Biology

    Antimicrobial peptide expression in a wild tobacco plant reveals the limits of host-microbe-manipulations in the field

    Arne Weinhold, Elham Karimi Dorcheh ... Ian T Baldwin

    The attempt to manipulate a microbiome in planta to study the ecological consequences under field conditions leaves plants and their microbes surprisingly unimpressed.
31. 1. Immunology and Inflammation

    Histone Deacetylase 7 mediates tissue-specific autoimmunity via control of innate effector function in invariant Natural Killer T Cells

    Herbert G Kasler, Intelly S Lee ... Eric Verdin

    The development of Natural Killer T Cells is controlled by Histone Deacetylase 7, a function that combined with its known role in thymic negative selection provides a potential mechanism explaining its association with tissue-specific autoimmunity in humans.
32. 1. Chromosomes and Gene Expression

    A genetic program mediates cold-warming response and promotes stress-induced phenoptosis in C. elegans

    Wei Jiang, Yuehua Wei ... Dengke K Ma

    A gene regulatory program regulating thermal stress response and organismic death.
33. 1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics

    Free-energy simulations reveal molecular mechanism for functional switch of a DNA helicase

    Wen Ma, Kevin D Whitley ... Klaus Schulten

    Integration of structural bioinformatics and free-energy simulations reveals how a helicase switches its function from unwinding to rezipping DNA, during which a key metastable conformation is predicted and verified by single-molecule measurements.
34. 1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h

    Nathan Gamarra, Stephanie L Johnson ... Geeta J Narlikar

    Auto-inhibition of ISWI-family ATP-dependent chromatin remodelers is relieved by a conserved feature of the nucleosome.
35. 1. Computational and Systems Biology
    2. Immunology and Inflammation

    Computer-guided design of optimal microbial consortia for immune system modulation

    Richard R Stein, Takeshi Tanoue ... Vanni Bucci

    A computational method is presented that quantifies the effect that specific bacteria in the gut have on the immune system and guides the design of therapeutically potent microbial consortia to cure auto-immune disease.
36. 1. Immunology and Inflammation

    Somatic hypermutation of T cell receptor α chain contributes to selection in nurse shark thymus

    Jeannine A Ott, Caitlin D Castro ... Michael F Criscitiello

    Shark's T cells suggest plasticity in diversification mechanisms used by vertebrate lymphocytes, including somatic hypermutation in the thymus.
37. 1. Neuroscience

    Control of voluntary and optogenetically perturbed locomotion by spike rate and timing of neurons of the mouse cerebellar nuclei

    Rashmi Sarnaik, Indira M Raman

    Stride-related modulated firing by neurons of the cerebellar nuclei is required for smooth execution of practiced locomotion and persists more easily with synchronous than asynchronous Purkinje-mediated inhibition.
38. 1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Crystal structure of intraflagellar transport protein 80 reveals a homo-dimer required for ciliogenesis

    Michael Taschner, Anna Lorentzen ... Esben Lorentzen

    CRISPR/Cas knockout of intraflagellar transport protein 80 shows that this subunit is absolutely required for ciliogenesis, and biophysical studies reveal that this protein may dimerize the intraflagellar transport complex.
39. 1. Cell Biology

    Transcriptomic analyses reveal rhythmic and CLOCK-driven pathways in human skeletal muscle

    Laurent Perrin, Ursula Loizides-Mangold ... Charna Dibner

    Rhythmic transcriptome analyses of human skeletal muscle tissue and cultured primary myotubes reveal an essential role for the circadian coordination of glucose homeostasis and lipid metabolism in human skeletal muscle.
40. 1. Neuroscience

    First spikes in visual cortex enable perceptual discrimination

    Arbora Resulaj, Sarah Ruediger ... Massimo Scanziani

    A brief time window of visually evoked activity in mouse primary visual cortex is sufficient for perceptual discrimination.
41. 1. Developmental Biology

    Negative regulation of G2-M by ATR (mei-41)/Chk1(Grapes) facilitates tracheoblast growth and tracheal hypertrophy in Drosophila

    Amrutha Kizhedathu, Archit V Bagul, Arjun Guha

    ATR/Chk1 contribute to G2 arrest in developing tracheoblasts, and arrest in G2 facilitates cellular and hypertrophic organ growth.
42. 1. Neuroscience

    Response to comment on "Magnetosensitive neurons mediate geomagnetic orientation in Caenorhabditis elegans"

    Andres Vidal-Gadea, Chance Bainbridge ... Jonathan Pierce-Shimomura

    A reanalysis of data from a challenge by Landler et al. (2018) and our Vidal-Gadea et al. (2015) study reinforce our original finding that C. elegans is a suitable model system to study magnetoreception.
43. 1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    IFN-λ prevents influenza virus spread from the upper airways to the lungs and limits virus transmission

    Jonas Klinkhammer, Daniel Schnepf ... Peter Staeheli

    Interferon-λ plays a decisive and previously underestimated role in limiting the spread of respiratory viruses from the nasal cavity to the lungs and it efficiently restricts virus transmission from infected individuals to naïve contacts.
44. 1. Structural Biology and Molecular Biophysics

    Microtubule assembly governed by tubulin allosteric gain in flexibility and lattice induced fit

    Maxim Igaev, Helmut Grubmüller

    A new combined mechanism explains how GTP binding by tubulin is linked to its dynamics and energetics in solution and why GTP-induced flexibility, and not the tubulin conformation as such, drives microtubule assembly.
45. 1. Neuroscience

    Motor selection dynamics in FEF explain the reaction time variance of saccades to single targets

    Christopher K Hauser, Dantong Zhu ... Emilio Salinas

    Oculomotor circuits are always busy planning the next eye movement, and this explains why, when a visual target appears, some eye movements toward it are produced very quickly whereas others take a long time to prepare.
46. 1. Neuroscience

    Comment on "Magnetosensitive neurons mediate geomagnetic orientation in Caenorhabditis elegans"

    Lukas Landler, Simon Nimpf ... David A Keays

    Employing blinded controlled methodology we find no evidence for a magnetic sense in the nematode worm Caenorhabditis elegans.
47. 1. Neuroscience

    Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity

    Zohreh Farsi, Sindhuja Gowrisankaran ... Ira Milosevic

    Parallel measurements of pH gradient and membrane potential at the single vesicle level have revealed that the synaptic vesicle acidification is initiated by removal of its clathrin coat, which blocks vesicular ATPase activity.
48. 1. Chromosomes and Gene Expression

    Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts

    Jason C Bell, David Jukam ... Aaron F Straight

    ChAR-seq is a massively parallelized de novo RNA mapping assay, which is capable of generating hundreds to thousands of RNA-binding maps with no a priori knowledge of target RNAs.
49. 1. Genetics and Genomics

    Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage

    Carla Gonçalves, Jennifer H Wisecaver ... Paula Gonçalves

    Comparative genomics coupled with genetic and biochemical studies show that central carbon metabolism in fungi can be remodeled by genes originating from bacteria.
50. 1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    The conformation of the histone H3 tail inhibits association of the BPTF PHD finger with the nucleosome

    Emma A Morrison, Samuel Bowerman ... Catherine A Musselman

    The BPTF PHD finger is inhibited from binding the methylated H3 tails in the context of the nucleosome due to their robust interaction with the nucleosomal DNA, which can be modulated by additional histone PTMs.
51. 1. Cancer Biology
    2. Cell Biology

    Wnt5a signaling induced phosphorylation increases APT1 activity and promotes melanoma metastatic behavior

    Rochelle Shirin Sadeghi, Katarzyna Kulej ... Eric S Witze

    Phosphorylation of serine residues 209 and 210 on APT1 increases its depalmitoylating activity, promoting Wnt5a mediated melanoma cell invasion.
52. 1. Evolutionary Biology

    Silencing of transposable elements may not be a major driver of regulatory evolution in primate iPSCs

    Michelle C Ward, Siming Zhao ... Yoav Gilad

    Human and chimpanzee-induced pluripotent stem cells show limited species-specificity in transposable element silencing.
53. 1. Computational and Systems Biology

    The genetic landscape of a physical interaction

    Guillaume Diss, Ben Lehner

    First comprehensive analysis of how many different mutations combine within and between two genes to alter a molecular phenotype.
54. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Two distinct DNA sequences recognized by transcription factors represent enthalpy and entropy optima

    Ekaterina Morgunova, Yimeng Yin ... Jussi Taipale

    The epistasis observed in TF-DNA binding preferences can be explained by the presence of two optima of very similar Gibbs energy that are located relatively far from each other in sequence space.
55. 1. Cell Biology
    2. Developmental Biology

    An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

    Pooran Singh Dewari, Benjamin Southgate ... Steven M Pollard

    Biochemical tags can be easily knocked into endogenous genes in mammalian stem cells using optimised and scalable protocols that will enable annotation of protein levels, localisation and interaction partners.
56. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    IRS-1 acts as an endocytic regulator of IGF-I receptor to facilitate sustained IGF signaling

    Yosuke Yoneyama, Peter Lanzerstorfer ... Shin-Ichiro Takahashi

    IRS-1, a canonical signaling mediator of IGF-I receptor, serves as an endocytic regulator of IGF-I receptor to facilitate the sustained IGF signaling.
57. 1. Neuroscience

    Glutamatergic drive along the septo-temporal axis of hippocampus boosts prelimbic oscillations in the neonatal mouse

    Joachim Ahlbeck, Lingzhen Song ... Ileana L Hanganu-Opatz

    Combined optogenetics and electrophysiology in vivo identified the cellular substrate of hippocampal drive that initiates the oscillatory entrainment of prefrontal cortex in neonatal mice.
58. 1. Neuroscience

    Proprioceptive and cutaneous sensations in humans elicited by intracortical microstimulation

    Michelle Armenta Salas, Luke Bashford ... Richard A Andersen

    Electrical microstimulation of human somatosensory cortex elicited purely naturalistic proprioceptive and cutaneous sensations, with proprioceptive sensations more prominent at higher current amplitudes suggesting a relationship between amplitude and sensation type.
59. 1. Neuroscience

    Dynamic representation of 3D auditory space in the midbrain of the free-flying echolocating bat

    Ninad B Kothari, Melville J Wohlgemuth, Cynthia F Moss

    Neurons in the midbrain superior colliculus of free-flying echolocating bats represent 3D sensory space, and the depth tuning of single neurons is modulated by an animal's active sonar inspection of physical objects in its environment.
60. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces

    Vladimir A Volkov, Pim J Huis in 't Veld ... Andrea Musacchio

    Multiple NDC80 complexes in the outer kinetochore cooperate to bind the ends of depolymerizing microtubules and harness their power stroke.
61. 1. Developmental Biology

    A population of adult satellite-like cells in Drosophila is maintained through a switch in RNA-isoforms

    Hadi Boukhatmi, Sarah Bray

    Adult muscle progenitors evade differentiation by expressing a short RNA isoform of Zfh1 which is insensitive to regulation by a micro-RNA.
62. 1. Cell Biology
    2. Developmental Biology

    Radical and lunatic fringes modulate notch ligands to support mammalian intestinal homeostasis

    Preetish Kadur Lakshminarasimha Murthy, Tara Srinivasan ... Xiling Shen

    Fringe proteins regulate Notch pathway differentially in the stem cell zone and progenitor compartment of the mouse intestinal epithelium to promote homeostasis.
63. 1. Cell Biology
    2. Neuroscience

    Small molecule induced oligomerization, clustering and clathrin-independent endocytosis of the dopamine transporter

    Tatiana Sorkina, Shiqi Ma ... Alexander Sorkin

    Dopamine transporter (DAT) interaction with furopyrimidine called AIM-100 in intact cells leads to dramatic oligomerization, nanoclustering and endocytosis of DAT by a novel mechanism coupled to the transporter molecule conformation.
64. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    RETRACTED: Proteasome storage granules protect proteasomes from autophagic degradation upon carbon starvation

    Richard S Marshall, Richard D Vierstra

    Proteasomes are protected from autophagic elimination upon carbon starvation by sequestration into cytoplasmic storage granules, which aid cell fitness by providing a cache of proteasomes that can be rapidly remobilized when carbon availability improves.
65. 1. Cell Biology

    Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish

    Sharan Janjuha, Sumeet Pal Singh ... Nikolay Ninov

    During aging, the pancreatic endocrine islets undergo chronic inflammation, which is associated with a reduced capacity for self-renewal of the insulin-producing beta-cells in zebrafish.
66. 1. Developmental Biology

    Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock

    Laura Lleras Forero, Rachna Narayanan ... Stefan Schulte-Merker

    In contrast to amniotes, zebrafish (ray-finned fish, teleost) centra are formed from specialised notochord sheath cells, and the segmental patterning of these cells is independent of the segmentation clock.
67. 1. Neuroscience

    Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation

    Xiling Li, Pragya Goel ... Dion K Dickman

    Genetic and electrophysiological analyses reveal that the mechanisms orchestrating the induction and expression of homeostatic plasticity are compartmentalized and operate with exquisite specificity on both sides of the synapse.
68. 1. Developmental Biology

    VEGF-C promotes the development of lymphatics in bone and bone loss

    Devon Hominick, Asitha Silva ... Michael T Dellinger

    Mice that overexpress VEGF-C in bone display a phenotype that resembles Gorham-Stout disease.
69. 1. Cell Biology
    2. Computational and Systems Biology

    Integrating images from multiple microscopy screens reveals diverse patterns of change in the subcellular localization of proteins

    Alex X Lu, Yolanda T Chong ... Alan M Moses

    100,000s of images from different growth conditions and genetic backgrounds can be integrated into proteome-scale analysis.
70. 1. Cancer Biology

    MPDZ promotes DLL4-induced Notch signaling during angiogenesis

    Fabian Tetzlaff, M Gordian Adam ... Andreas Fischer

    The multiple PDZ domain protein recruits Notch ligands to Nectin-2, which increases Notch signaling activity during angiogenesis.
71. 1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    A robust and tunable mitotic oscillator in artificial cells

    Ye Guan, Zhengda Li ... Qiong Yang

    The development of a robust and tunable artificial cell-cycle system paves the way for quantitative characterization of complex clock functions.
72. 1. Computational and Systems Biology
    2. Chromosomes and Gene Expression

    Stochastic tuning of gene expression enables cellular adaptation in the absence of pre-existing regulatory circuitry

    Peter L Freddolino, Jamie Yang ... Saeed Tavazoie

    Stochastic tuning of gene expression could be a common mechanism through which eukaryotic cells adapt to challenging external environments, potentially including survival of infectious organisms within the host and adaptation of cancer cells to chemotherapy.
73. 1. Neuroscience

    Shared rhythmic subcortical GABAergic input to the entorhinal cortex and presubiculum

    Tim James Viney, Minas Salib ... Peter Somogyi

    GABAergic parvalbumin-expressing 'orchid cells' of the medial septum selectively target GABAergic neurons in connected extra-hippocampal areas of the mouse, providing rhythmically bursting inhibitory input at the peak of hippocampal 5-12 Hz theta oscillatory cycles.
74. 1. Genetics and Genomics

    An incoherent feedforward loop facilitates adaptive tuning of gene expression

    Jungeui Hong, Nathan Brandt ... David Gresham

    The architecture of a gene regulatory network determines the effect of evolutionary changes in transcription factor binding.
75. 1. Neuroscience

    Formation of retinal direction-selective circuitry initiated by starburst amacrine cell homotypic contact

    Thomas A Ray, Suva Roy ... Jeremy N Kay

    Selective synapse formation in a retinal motion-sensitive circuit is orchestrated by starburst amacrine cells, which use homotypic interactions to initiate formation of a dendritic scaffold that recruits projections from circuit partners.
76. 1. Developmental Biology
    2. Neuroscience

    Neural retina-specific Aldh1a1 controls dorsal choroidal vascular development via Sox9 expression in retinal pigment epithelial cells

    So Goto, Akishi Onishi ... Masayo Takahashi

    Neural retina-derived retinoic acids synthesized by Aldh1a1 control choroidal vascular development by regulating RPE-secreted VEGF.
77. 1. Chromosomes and Gene Expression

    MCM2–7-dependent cohesin loading during S phase promotes sister-chromatid cohesion

    Ge Zheng, Mohammed Kanchwala ... Hongtao Yu

    Systematic analyses of DNA replication machinery components in human cells reveal a requirement of MCM-dependent de novo loading or mobilization of cohesin at replication forks in establishing sister-chromatid cohesion.
78. 1. Neuroscience

    Fast and accurate edge orientation processing during object manipulation

    J Andrew Pruszynski, J Randall Flanagan, Roland S Johansson

    Tactile edge orientation processing during object manipulation is many times better than previously thought based on perceptual studies.
79. 1. Biochemistry and Chemical Biology
    2. Neuroscience

    CK1/Doubletime activity delays transcription activation in the circadian clock

    Deniz Top, Jenna L O'Neil ... Michael W Young

    Doubletime kinase stabilizes the PER/TIM complex and regulates its transcriptional inhibition function to delay circadian transcriptional activity, helping sustain 24-hour periodicity in the circadian clock.
80. 1. Neuroscience

    Large-scale replication study reveals a limit on probabilistic prediction in language comprehension

    Mante S Nieuwland, Stephen Politzer-Ahles ... Falk Huettig

    Large-scale replication study with brain potentials challenges the view that people routinely predict the phonological form of a predictable word during language comprehension.
81. 1. Structural Biology and Molecular Biophysics

    Functional roles of Mg²⁺ binding sites in ion-dependent gating of a Mg²⁺ channel, MgtE, revealed by solution NMR

    Tatsuro Maruyama, Shunsuke Imai ... Masanori Osawa

    Nuclear magnetic resonance analysis revealed the structural and dynamical changes in MgtE during the channel closure caused by the cooperative Mg²⁺-binding, which had remained undescribed only by a static crystal structure.