December 2019

Cover articles

    1. Neuroscience
    2. Stem Cells and Regenerative Medicine

    Splicing defects and Timothy syndrome

    Georgia Panagiotakos, Christos Haveles ... Ricardo E Dolmetsch
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Regulating the length of flagella

    Shane G McInally, Jane Kondev, Scott C Dawson
    1. Evolutionary Biology
    2. Neuroscience

    Evolution of pheromone receptors

    Lucie Bastin-Héline, Arthur de Fouchier ... Nicolas Montagné

Highlights controls:

Research articles

    1. Developmental Biology
    2. Neuroscience

    Activity-dependent death of transient Cajal-Retzius neurons is required for functional cortical wiring

    Martina Riva, Ioana Genescu ... Alessandra Pierani
    The complete elimination of a transient superficial population of neurons, and associated immature circuits, is required for the emergence of functional properties in the mature cerebral cortex.
    1. Chromosomes and Gene Expression

    Cdc7 activates replication checkpoint by phosphorylating the Chk1-binding domain of Claspin in human cells

    Chi-Chun Yang, Hiroyuki Kato ... Hisao Masai
    Cdc7 and CK1g1 independently and additively phosphorylate the Chk1-binding domain of claspin to activate replication checkpoint with differential contribution of each kinase in different cell types.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural basis for the activation of PLC-γ isozymes by phosphorylation and cancer-associated mutations

    Nicole Hajicek, Nicholas C Keith ... John Sondek
    The high-resolution X-ray crystal structure of PLC-γ1 reveals a unifying model for the regulation of the PLC-γ isozymes.
    1. Structural Biology and Molecular Biophysics

    The structure of the endogenous ESX-3 secretion system

    Nicole Poweleit, Nadine Czudnochowski ... Oren S Rosenberg
    The structure of the endogenous ESX-3 secretion system reveals a highly coordinated inner membrane complex and suggests a mechanism for the regulated secretion of ESX substrates.
    1. Epidemiology and Global Health

    Estimating effectiveness of case-area targeted response interventions against cholera in Haiti

    Edwige Michel, Jean Gaudart ... Stanislas Rebaudet
    Case-area targeted interventions conducted by rapid response teams appeared significantly effective to mitigate and shorten local cholera outbreaks in the Centre department of Haiti in 2015-2017.
    1. Structural Biology and Molecular Biophysics

    Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement

    Avinash B Patel, Camille M Moore ... Eva Nogales
    The structure of the yeast RSC complex, a member of the SWI/SNF chromatin remodeling family, determined by cryo-electron microscopy, reveals a conserved structural core and the mode of nucleosome engagement.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    The Ormdl genes regulate the sphingolipid synthesis pathway to ensure proper myelination and neurologic function in mice

    Benjamin A Clarke, Saurav Majumder ... Richard L Proia
    The ORMDL proteins function to restrain the de novo sphingolipid biosynthetic pathway during myelination, when there is a high demand for sphingolipids to prevent excessive accumulation of metabolic intermediates.
    1. Structural Biology and Molecular Biophysics

    Molecular basis of force-from-lipids gating in the mechanosensitive channel MscS

    Bharat Reddy, Navid Bavi ... Eduardo Perozo
    The structure of the mechanosensitive channel MscS embedded in a lipid bilayer redefines the nature, location and importance of lipid–protein interactions in the gating of mechanosensitive channels.
    1. Neuroscience

    High and asymmetric somato-dendritic coupling of V1 layer 5 neurons independent of visual stimulation and locomotion

    Valerio Francioni, Zahid Padamsey, Nathalie L Rochefort
    Layer 5 neuron apical tuft in mouse visual cortex display widespread, highly correlated calcium signals, with a strong and asymmetric coupling to somatic signals, independent of visual stimulation and locomotion.
    1. Cell Biology

    Unstructured regions in IRE1α specify BiP-mediated destabilisation of the luminal domain dimer and repression of the UPR

    Niko Amin-Wetzel, Lisa Neidhardt ... David Ron
    Client protein-driven reversal of endoplasmic reticulum chaperone (BiP) mediated-repression is revealed as a principal component of the regulation of the unfolded protein response transducer IRE1 in cells.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Subcellular drug targeting illuminates local kinase action

    Paula J Bucko, Chloe K Lombard ... John D Scott
    Genetically-encoded platforms direct kinase inhibitor drugs to organelles to reveal new dimensions of local kinase signaling.
    1. Computational and Systems Biology
    2. Microbiology and Infectious Disease

    Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions

    Luis V Nobre, Katie Nightingale ... Michael P Weekes
    The human cytomegalovirus (HCMV) interactome systematically characterises high-confidence viral-viral and viral-host protein interactions in HCMV-infected cells, facilitating multiple novel insights into HCMV and herpesviral function.
    1. Cancer Biology

    Siglec1-expressing subcapsular sinus macrophages provide soil for melanoma lymph node metastasis

    Rohit Singh, Beom K Choi
    When pioneer metastatic cells enter a sentinel lymph node through afferent lymphatics subcapsular sinus, macrophages provide them soil to proliferate and colonize lymph node.
    1. Neuroscience

    Spinal signalling of C-fiber mediated pleasant touch in humans

    Andrew G Marshall, Manohar L Sharma ... Francis P McGlone
    Pleasant touch perception in humans is unaffected by spinothalamic disruption indicating integrated spinal processing of hedonic and discriminative tactile inputs rather than privileged C-tactile-lamina I-spinothalamic projections.
    1. Neuroscience

    Relaxation of synaptic inhibitory events as a compensatory mechanism in fetal SOD spinal motor networks

    Pascal Branchereau, Elodie Martin ... Daniel Cattaert
    Electrophysiological and simulation approaches show that a chloride-related longer relaxation of the inhibitory synaptic events partially compensates the early defect in the chloride homeostasis detected in fetal SOD spinal motoneurons.
    1. Neuroscience

    A multilayer circuit architecture for the generation of distinct locomotor behaviors in Drosophila

    Aref Arzan Zarin, Brandon Mark ... Chris Q Doe
    Generation of a premotor/motor neuron comprehensive TEM reconstruction, functional optogenetics, and recurrent network modeling reveals different phase relationships among a subset of Drosophila motor neurons in forward versus backward locomotion.
    1. Cell Biology
    2. Computational and Systems Biology

    Development, calibration, and validation of a novel human ventricular myocyte model in health, disease, and drug block

    Jakub Tomek, Alfonso Bueno-Orovio ... Blanca Rodriguez
    A computer model of human cardiomyocyte was produced and validated on independent datasets, overcoming shortcomings of its predecessors, also yielding broadly relevant insights and results on major ionic currents.
    1. Cell Biology
    2. Developmental Biology

    Metabolic modulation regulates cardiac wall morphogenesis in zebrafish

    Ryuichi Fukuda, Alla Aharonov ... Didier YR Stainier
    3D single cell imaging and genetic analyses reveal a new role of cardiac metabolism in cardiac wall morphogenesis.
    1. Structural Biology and Molecular Biophysics

    Activation of a nucleotide-dependent RCK domain requires binding of a cation cofactor to a conserved site

    Celso M Teixeira-Duarte, Fátima Fonseca, João H Morais-Cabral
    Cations are essential cofactors in the ATP activation mechanism of the KtrAB cation channel and appear to be cofactors of many other nucleotide-dependent RCK domains.
    1. Neuroscience

    Homeostatic regulation of perisynaptic matrix metalloproteinase 9 (MMP9) activity in the amblyopic visual cortex

    Sachiko Murase, Dan Winkowski ... Elizabeth M Quinlan
    Dark exposure lowers the MMP9 activation threshold, and subsequent light stimulation to an amblyopic eye is sufficient to induce proteolysis at thalamo-cortical synapses in deprived mouse visual cortex.
    1. Cell Biology
    2. Neuroscience

    Sensing of nutrients by CPT1C regulates late endosome/lysosome anterograde transport and axon growth

    Marta Palomo-Guerrero, Rut Fadó ... Núria Casals
    The pseudoenzyme CPT1C is able to sense changes in intracellular malonyl-CoA levels caused by nutrients or energy stress and regulate late endosomes/lysosomes anterograde transport, necessary for proper axon growth.
    1. Developmental Biology

    VEGF/VEGFR2 signaling regulates hippocampal axon branching during development

    Robert Luck, Severino Urban ... Carmen Ruiz de Almodóvar
    Hippocampal axon branching, required for the formation of functional neuronal networks, is regulated by VEGF/VEGFR2 signaling during development.
    1. Neuroscience
    2. Stem Cells and Regenerative Medicine

    Aberrant calcium channel splicing drives defects in cortical differentiation in Timothy syndrome

    Georgia Panagiotakos, Christos Haveles ... Ricardo E Dolmetsch
    The Timothy syndrome mutation in Cav1.2 gives rise to defects in neuronal differentiation by preventing a developmental switch in channel splicing and elevating calcium signaling in differentiating cells.
    1. Evolutionary Biology
    2. Structural Biology and Molecular Biophysics

    Indirect sexual selection drives rapid sperm protein evolution in abalone

    Damien Beau Wilburn, Lisa M Tuttle ... Willie J Swanson
    An NMR-based structural study of sperm proteins reveals a novel protein packaging/dispersion system embedded in a coevolutionary arms race.
    1. Developmental Biology
    2. Evolutionary Biology

    Resolving homology in the face of shifting germ layer origins: Lessons from a major skull vault boundary

    Camilla S Teng, Lionel Cavin ... J Gage Crump
    Homology of vertebrate skull structures should be based on evolutionary continuity and an appreciation of germ layer origins and inductive signaling in the embryonic head.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart

    Hessel Honkoop, Dennis EM de Bakker ... Jeroen Bakkers
    Single-cell RNA sequencing reveals Nrg1/ErbB2-induced metabolic reprogramming as a pivotal event for cardiomyocyte proliferation during heart regeneration.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Robust cullin-RING ligase function is established by a multiplicity of poly-ubiquitylation pathways

    Spencer Hill, Kurt Reichermeier ... Gary Kleiger
    Human cullin-RING ligases are buffered to a much greater extent than had been previously appreciated, and the roles of ubiquitin chain extension enzymes are far more nuanced at physiological concentrations.
    1. Neuroscience

    Acute cerebellar knockdown of Sgce reproduces salient features of myoclonus-dystonia (DYT11) in mice

    Samantha Washburn, Rachel Fremont ... Kamran Khodakhah
    Acute knock down of Sgce that is mutated in Myoclonus Dystonia (DYT11), in the cerebellum of mice, leads to dystonia and myoclonus-like motor signs that like patients improve with alcohol.
    1. Cell Biology
    2. Immunology and Inflammation

    A role for phagocytosis in inducing cell death during thymocyte negative selection

    Nadia S Kurd, Lydia K Lutes ... Ellen A Robey
    Self-reactive thymocyte death is most efficient when peptide-presenting cells are phagocytic, and blocking phagocytosis inhibits self-reactive thymocyte death, suggesting that phagocytes play central roles during thymocyte negative selection.
    1. Cancer Biology
    2. Developmental Biology

    Regulation of zebrafish melanocyte development by ligand-dependent BMP signaling

    Alec K Gramann, Arvind M Venkatesan ... Craig J Ceol
    The oncogenic role ligand-dependent BMP signaling plays in suppressing differentiation in melanoma is a reiteration of its physiological roles during melanocyte development.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    C-mannosylation supports folding and enhances stability of thrombospondin repeats

    Aleksandra Shcherbakova, Matthias Preller ... Hans Bakker
    C-mannosylation supports native folding of thrombospondin type 1 repeats in the endoplasmic reticulum and stabilizes the folded proteins by modulating the dynamics of the tryptophan-arginine ladder.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    MeCP2 nuclear dynamics in live neurons results from low and high affinity chromatin interactions

    Francesco M Piccolo, Zhe Liu ... Nathaniel Heintz
    Single molecular imaging of MeCP2 at high spatial/temporal resolution identifies distinct structural domains contributing to its dynamic behaviors and chromatin interactions in live neurons.
    1. Developmental Biology
    2. Neuroscience

    EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development

    Eva Harde, LaShae Nicholson ... Amparo Acker-Palmer
    The internalization of the angiogenic receptor VEGFR2 expressed in neurons is controlled by ephrinB2 and is required for neuronal dendritic arborization, spine morphogenesis and circuitry development in the hippocampus.
    1. Computational and Systems Biology
    2. Neuroscience

    From single neurons to behavior in the jellyfish Aurelia aurita

    Fabian Pallasdies, Sven Goedeke ... Raoul-Martin Memmesheimer
    A multiscale computational nerve net model describes how the activity of individual neurons controls the swimming motion of a jellyfish in its hydrodynamic environment.
    1. Neuroscience

    FMRP has a cell-type-specific role in CA1 pyramidal neurons to regulate autism-related transcripts and circadian memory

    Kirsty Sawicka, Caryn R Hale ... Robert B Darnell
    Identifying FMRP-bound mRNAs in hippocampal CA1 pyramidal neurons reveals cell-type specific regulation of autism-candidate and circadian mRNAs and FMRP-mediated control of memory across the circadian cycle.
    1. Neuroscience

    Pathway-, layer- and cell-type-specific thalamic input to mouse barrel cortex

    B Semihcan Sermet, Pavel Truschow ... Carl CH Petersen
    Two distinct thalamocortical pathways were found to provide differential excitatory synaptic input to distinct cell-types across layers of mouse primary somatosensory barrel cortex.
    1. Cell Biology
    2. Physics of Living Systems

    Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis

    Michael M Lacy, David Baddeley, Julien Berro
    The main proteins of clathrin-mediated endocytosis bind and unbind rapidly, continuously turning over about five times during the formation of an endocytic vesicle in yeast.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Length-dependent disassembly maintains four different flagellar lengths in Giardia

    Shane G McInally, Jane Kondev, Scott C Dawson
    Giardia, a multi-ciliated parasitic protist, maintains four different flagellar lengths by balancing flagellar length-independent IFT-mediated assembly with length-dependent kinesin-13 mediated disassembly.
    1. Structural Biology and Molecular Biophysics

    De novo design of a homo-trimeric amantadine-binding protein

    Jooyoung Park, Brinda Selvaraj ... David Baker
    The first successful de novo design of a homo-trimeric protein that binds a C3 symmetric small molecule larger than a metal ion is an advance for computational protein design.
    1. Cancer Biology

    TLE3 loss confers AR inhibitor resistance by facilitating GR-mediated human prostate cancer cell growth

    Sander AL Palit, Daniel Vis ... Michiel S van der Heijden
    A mechanistic link between TLE3 loss and glucocorticoid receptor-mediated androgen receptor inhibitor resistance supports the rationale to target GR during anti-hormonal treatment in castrate-resistant prostate cancer.
    1. Computational and Systems Biology
    2. Structural Biology and Molecular Biophysics

    Common activation mechanism of class A GPCRs

    Qingtong Zhou, Dehua Yang ... Suwen Zhao
    Contrary to previous findings, class A GPCRs share a common activation pathway that directly links ligand binding to G-protein activation, as revealed by novel quantitative analysis.
    1. Structural Biology and Molecular Biophysics

    Large-scale state-dependent membrane remodeling by a transporter protein

    Wenchang Zhou, Giacomo Fiorin ... José D Faraldo-Gómez
    The conformational mechanism of a transporter protein is found to have profound and energetically costly effects on the morphology of the surrounding membrane.
    1. Chromosomes and Gene Expression

    GC content shapes mRNA storage and decay in human cells

    Maïté Courel, Yves Clément ... Dominique Weil
    The GC content of human mRNAs is key to P-body localization and protein yield, and has a major impact on their post-transcriptional control.
    1. Chromosomes and Gene Expression

    The Fml1-MHF complex suppresses inter-fork strand annealing in fission yeast

    Io Nam Wong, Jacqueline PS Neo ... Matthew C Whitby
    Genomic deletions formed from inter-fork strand annealing associated with a collapsed replication fork are suppressed by the FANCM-related DNA helicase Fml1 in conjunction with its partner proteins Mhf1 and Mhf2.
    1. Neuroscience

    Uncovering the functional anatomy of the human insula during speech

    Oscar Woolnough, Kiefer James Forseth ... Nitin Tandon
    Direct insular recordings in humans reveal that contrary to several prominent models of speech production, it is not engaged in pre-articulatory planning, but in auditory and somatosensory components of speech.
    1. Stem Cells and Regenerative Medicine

    The follicle epithelium in the Drosophila ovary is maintained by a small number of stem cells

    Jocelyne Fadiga, Todd G Nystul
    Just 2-4 follicle stem cells maintain each follicle epithelium in the Drosophila ovary, and they reside within a single ring at the anterior edge of the tissue.
    1. Neuroscience

    Inferring synaptic inputs from spikes with a conductance-based neural encoding model

    Kenneth W Latimer, Fred Rieke, Jonathan W Pillow
    The conductance-based encoding model creates a new bridge between statistical models and biophysical models of neurons, and infers visually-evoked excitatory and inhibitory synaptic conductances from spike trains in macaque retina.
    1. Neuroscience

    Catecholaminergic modulation of meta-learning

    Jennifer L Cook, Jennifer C Swart ... Roshan Cools
    A human psychopharmacology study reveals that a drug that affects the dopamine and noradrenaline systems enhances people's ability to adapt their learning rate to suit the volatility of the environment.
    1. Structural Biology and Molecular Biophysics

    HIV restriction factor APOBEC3G binds in multiple steps and conformations to search and deaminate single-stranded DNA

    Michael Morse, M Nabuan Naufer ... Mark C Williams
    APOBEC3G, an anti-viral protein with the capability to inhibit HiV-1 infectivity, binds single stranded DNA in multiple physical conformations, enabling complex interactions that allow APOBEC3G to perform multiple diverse functions.
    1. Neuroscience

    Invariant representations of mass in the human brain

    Sarah Schwettmann, Joshua B Tenenbaum, Nancy Kanwisher
    A network of brain regions implicated in physical inference represents abstract, generalizable variables used as inputs to physics engines.
    1. Ecology
    2. Epidemiology and Global Health

    Antagonism between parasites within snail hosts impacts the transmission of human schistosomiasis

    Martina R Laidemitt, Larissa C Anderson ... Eric S Loker
    Interactions among parasites in snails create cascading effects on their transmission and highlight that biodiversity has complex, context-dependent and important effects on human schistosomiasis transmission dynamics.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila development

    Satyam P Srivastav, Reazur Rahman ... Nelson C Lau
    A female fertility syndrome in Drosophila called gonadal dysgenesis is caused by the P-transposase actively mobilizing a very short P-element variant that has been named the Har-P.
    1. Chromosomes and Gene Expression

    Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor

    Juncao Xu, Kaijie Cui ... Yu Zhang
    The cryo-EM structure of Escherichia coli Crl–TAC reveals the mechanism of DNA-contact-independent transcription activation.
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Combining genomics and epidemiology to analyse bi-directional transmission of Mycobacterium bovis in a multi-host system

    Joseph Crispell, Clare H Benton ... Rowland Raymond Kao
    Analyses combining genomic and epidemiological data of Mycobacterium bovis, which causes bovine tuberculosis, revealed evidence of transmission within and between cattle and badger populations.
    1. Physics of Living Systems
    2. Structural Biology and Molecular Biophysics

    Efficient conversion of chemical energy into mechanical work by Hsp70 chaperones

    Salvatore Assenza, Alberto Stefano Sassi ... Alessandro Barducci
    A multiscale modeling approach reveals how the energy from ATP hydrolysis is used by Hsp70 chaperones to remodel the conformation of their substrates through a novel force-generating mechanism.
    1. Developmental Biology

    Primary cilia deficiency in neural crest cells models anterior segment dysgenesis in mouse

    Céline Portal, Panteleimos Rompolas ... Carlo Iomini
    Primary cilia of neural crest-derived cells mediate Indian hedgehog-induced signal transduction in the periocular mesenchyme and are required for normal anterior segment development.
    1. Computational and Systems Biology
    2. Medicine

    Single-cell modeling of routine clinical blood tests reveals transient dynamics of human response to blood loss

    Anwesha Chaudhury, Geoff D Miller ... John M Higgins
    Unsteady state modeling of blood cell populations reveals that blood loss triggers a cellular clearance delay and a cellular production increase, and modeling provides earlier detection and individualized patient characterization.
    1. Neuroscience

    Default mode-visual network hypoconnectivity in an autism subtype with pronounced social visual engagement difficulties

    Michael V Lombardo, Lisa Eyler ... Karen Pierce
    Functional hypoconnectivity between ‘social brain’ default mode circuitry and visual association cortex underpins a subtype of autistic toddlers with a strong preference to attend to the non-social visual world.
    1. Cancer Biology

    Replication Study: Biomechanical remodeling of the microenvironment by stromal caveolin-1 favors tumor invasion and metastasis

    Mee Rie Sheen, Jennifer L Fields ... Reproducibility Project: Cancer Biology
    Editors' Summary: This Replication Study has reproduced some parts of the original paper but other parts could not be interpreted.
    1. Plant Biology

    Compensatory sequence variation between trans-species small RNAs and their target sites

    Nathan R Johnson, Claude W dePamphilis, Michael J Axtell
    Superfamilies of trans-species small RNAs from the parasitic plant Cuscuta have sequence variation that correspond to synonymous site variation in host plant target mRNAs.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Cell-based HTS identifies a chemical chaperone for preventing ER protein aggregation and proteotoxicity

    Keisuke Kitakaze, Shusuke Taniuchi ... Seiichi Oyadomari
    Cell-based high-throughput screening identifies IBT21 as a chemical chaperone, that inhibits ER protein aggregation and prevents the cell death caused by a proteotoxin, the aggregation-prone prion protein.
    1. Ecology

    Tritrophic metabolism of plant chemical defenses and its effects on herbivore and predator performance

    Ruo Sun, Xingcong Jiang ... Daniel Giddings Vassão
    Genetic manipulation demonstrates the costs and benefits of detoxifying glucosinolate plant defenses in a pest insect and its predator.
    1. Neuroscience

    Navigating the garden of forking paths for data exclusions in fear conditioning research

    Tina B Lonsdorf, Maren Klingelhöfer-Jens ... Christian J Merz
    Exclusion of participants in tasks with a learning element can introduce substantial bias and needs to be carefully considered and transparently reported and justified.
    1. Immunology and Inflammation

    Ribosome biogenesis restricts innate immune responses to virus infection and DNA

    Christopher Bianco, Ian Mohr
    Ribosome production is unexpectedly integrated into innate cell-intrinsic responses that regulate double strand DNA-sensing and inflammatory cytokine induction in infected and uninfected cells.
    1. Immunology and Inflammation

    Tissue-specific shaping of the TCR repertoire and antigen specificity of iNKT cells

    Rebeca Jimeno, Marta Lebrusant-Fernandez ... Patricia Barral
    The tissue environment shapes the lipid recognition capacity of natural killer T cells.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    E. coli TraR allosterically regulates transcription initiation by altering RNA polymerase conformation

    James Chen, Saumya Gopalkrishnan ... Seth A Darst
    Cryo-electron microscopy structures, combined with biochemical experiments, show how the E. coli F element-encoded TraR protein regulates transcription initiation by altering RNA polymerase conformation and conformational heterogeneity.
    1. Cancer Biology

    PCK1 and DHODH drive colorectal cancer liver metastatic colonization and hypoxic growth by promoting nucleotide synthesis

    Norihiro Yamaguchi, Ethan M Weinberg ... Sohail F Tavazoie
    The gluconeogenic enzyme PCK1 and pyrimidine nucleotide biosynthetic enzyme DHODH drive hypoxic pyrimidine nucleotide biosynthesis and liver metastatic colonization in colorectal cancer, which is therapeutically exploitable by DHODH pharmacologic inhibition.
    1. Neuroscience

    Opioids modulate an emergent rhythmogenic process to depress breathing

    Xiaolu Sun, Carolina Thörn Pérez ... Kaiwen Kam
    Inspiratory rhythmogenesis is mediated by an emergent mechanism independent of bursts associated with motor output and is modulated by opioids, pointing to strategies for ameliorating opioid-induced respiratory depression.
    1. Cancer Biology
    2. Cell Biology

    Axon-like protrusions promote small cell lung cancer migration and metastasis

    Dian Yang, Fangfei Qu ... Julien Sage
    In small cell lung cancer, the transition from a neuroendocrine state to a more neuronal state endows these cancer cells with increased migration and metastatic potential.
    1. Cell Biology
    2. Developmental Biology

    Telophase correction refines division orientation in stratified epithelia

    Kendall J Lough, Kevin M Byrd ... Scott E Williams
    Telophase reorientation corrects errors in spindle orientation that persist after imprecise initial spindle positioning during early mitosis, and contributes to balancing self-renewal with differentiation during epidermal development.
    1. Cell Biology

    Magnetic resonance measurements of cellular and sub-cellular membrane structures in live and fixed neural tissue

    Nathan H Williamson, Rea Ravin ... Peter J Basser
    Low-field single-sided magnetic resonance diffusion methods detect and measure permeability of sub-micron compartments which likely include cell processes, organelles, and cellular vesicles within ex vivo mouse spinal cords.
    1. Developmental Biology

    Developmentally regulated Tcf7l2 splice variants mediate transcriptional repressor functions during eye formation

    Rodrigo M Young, Kenneth B Ewan ... Stephen W Wilson
    The inclusion of a new alternatively spliced exon in Tcf7l2 can switch its function to repress transcription of Wnt target genes.
    1. Neuroscience

    Essential role for InSyn1 in dystroglycan complex integrity and cognitive behaviors in mice

    Akiyoshi Uezu, Erin Hisey ... Scott Soderling
    InSyn1 is a new inhibitory post-synaptic protein that is essential for the dystroglycan complex, neuronal activity in vitro and in vivo, and cognitive behavior.
    1. Developmental Biology

    Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1

    Emilie Auxerre-Plantié, Masayuki Nakamori ... Krzysztof Jagla
    Drosophila DM1 models suggest that elevated cardiac expression of straightjacket/α2δ3, a regulatory subunit of voltage-gated calcium channel, contributes to cardiac conduction defects in DM1.
    1. Genetics and Genomics

    Single cell transcriptional signatures of the human placenta in term and preterm parturition

    Roger Pique-Regi, Roberto Romero ... Nardhy Gomez-Lopez
    Parturition gene regulation across multiple cell-types and placental compartments is deciphered at single cell resolution, highlighting the common role of the NFKB pathway in both term and preterm birth.
    1. Cell Biology

    Direct comparison of clathrin-mediated endocytosis in budding and fission yeast reveals conserved and evolvable features

    Yidi Sun, Johannes Schöneberg ... David G Drubin
    Quantitative comparison of clathrin-mediated endocytosis in budding and fission yeast identified conserved mechanisms and species-specific adaptations with broad implications that extend from yeast to humans.
    1. Neuroscience

    Axon TRAP reveals learning-associated alterations in cortical axonal mRNAs in the lateral amygdala

    Linnaea E Ostroff, Emanuela Santini ... Eric Klann
    A diverse assortment of mRNAs are present in projection axons in the adult forebrain and their translation is associated with new memory formation.
    1. Neuroscience

    Agonist-specific voltage-dependent gating of lysosomal two-pore Na+ channels

    Xiaoli Zhang, Wei Chen ... Haoxing Xu
    Tricyclic antidepressants activate lysosomal two-pore Na+ channels in a voltage-dependent manner.
    1. Neuroscience

    The recovery of standing and locomotion after spinal cord injury does not require task-specific training

    Jonathan Harnie, Adam Doelman ... Alain Frigon
    Restoring locomotion after complete spinal cord injury does not require locomotor training, only the return of sufficient excitability within neurons of the spinal cord.
    1. Cell Biology
    2. Physics of Living Systems

    Rod nuclear architecture determines contrast transmission of the retina and behavioral sensitivity in mice

    Kaushikaram Subramanian, Martin Weigert ... Moritz Kreysing
    Establishing retinal contrast transmission as a novel determinant of mammalian fitness, this research adds functional significance to a prominent exception of nuclear organization, previously described in nocturnal rod photoreceptor cells.
    1. Genetics and Genomics

    Diversification of the Caenorhabditis heat shock response by Helitron transposable elements

    Jacob M Garrigues, Brian V Tsu ... Amy E Pasquinelli
    Helitron transposable elements, by providing high density transcription factor binding sites upstream of host genes, have diversified the heat shock response within and among Caenorhabditis species.
    1. Neuroscience

    MouseBytes, an open-access high-throughput pipeline and database for rodent touchscreen-based cognitive assessment

    Flavio H Beraldo, Daniel Palmer ... Marco AM Prado
    Comprehensive dataset of high-level cognitive assessment in mouse models of neurodegeneration, accompanied by an open-access database/repository to change the paradigm of how cognitive studies in animal models can be shared/re-used.
    1. Neuroscience

    The computation of directional selectivity in the Drosophila OFF motion pathway

    Eyal Gruntman, Sandro Romani, Michael B Reiser
    ON and OFF visual motion is computed with the same algorithm despite differences in circuit architecture.
    1. Neuroscience

    mTORC1 in the orbitofrontal cortex promotes habitual alcohol seeking

    Nadege Morisot, Khanhky Phamluong ... Dorit Ron
    In the orbitofrontal cortex, mTORC1, a multiprotein complex centered around the kinase mTOR, contributes to the development and/or maintenance of habitual alcohol seeking.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Broadly neutralizing human antibodies against dengue virus identified by single B cell transcriptomics

    Natasha D Durham, Aditi Agrawal ... Leslie Goo
    Identification of viral and host determinants of broadly neutralizing antibodies targeting a new epitope on dengue virus that can be used to guide vaccine design.
    1. Neuroscience

    Neural ensemble dynamics in dorsal motor cortex during speech in people with paralysis

    Sergey D Stavisky, Francis R Willett ... Jaimie M Henderson
    Neurons in human dorsal motor cortex, an area involved in controlling arm and hand movements, are also active – and show similar ensemble dynamics – during speaking.
    1. Computational and Systems Biology
    2. Epidemiology and Global Health

    Linking glycemic dysregulation in diabetes to symptoms, comorbidities, and genetics through EHR data mining

    Isa Kristina Kirk, Christian Simon ... Søren Brunak
    Text mining of complete EHRs for 14,017 diabetes patients and subsequent clustering led to phenotypically deep clusters, showing distinct glycemic profiles, comorbidities, and SNP association patterns.
    1. Evolutionary Biology
    2. Neuroscience

    A novel lineage of candidate pheromone receptors for sex communication in moths

    Lucie Bastin-Héline, Arthur de Fouchier ... Nicolas Montagné
    In moths, male receptors tuned to type I female pheromones may not have a monophyletic origin.
    1. Neuroscience

    Local cortical desynchronization and pupil-linked arousal differentially shape brain states for optimal sensory performance

    Leonhard Waschke, Sarah Tune, Jonas Obleser
    The momentary levels of local cortical desynchronization and pupil-linked arousal pose dissociable influences not only on the processing of sensory information but also on human perceptual performance.
    1. Evolutionary Biology

    Retinal oxygen supply shaped the functional evolution of the vertebrate eye

    Christian Damsgaard, Henrik Lauridsen ... Mark Bayley
    Multiple respiratory and vascular mechanisms have recurrently evolved across the vertebrates to alleviate the oxygen diffusion limitations inherent to the morphology of the retina.
    1. Biochemistry and Chemical Biology

    Long-lived metabolic enzymes in the crystalline lens identified by pulse-labeling of mice and mass spectrometry

    Pan Liu, Seby Louis Edassery ... Jing Jin
    By performing 15N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.
    1. Cell Biology
    2. Developmental Biology

    Dzip1 and Fam92 form a ciliary transition zone complex with cell type specific roles in Drosophila

    Jean-André Lapart, Marco Gottardo ... Bénédicte Durand
    Genetic analyses in Drosophila establish the functional hierarchy of the ciliary transition zone module, Dzip1/Fam92/Cby, and reveal tissue specific variations in basal body anchoring pathways in fly ciliated tissues.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxia

    Fransky Hantelys, Anne-Claire Godet ... Anne-Catherine Prats
    In mouse cardiomyocytes, (lymph)angiogenic growth factors are induced during early hypoxia by a translational mechanism involving a new IRES trans-acting factor, vasohibin-1.
    1. Structural Biology and Molecular Biophysics

    Molecular structures of the human Slo1 K+ channel in complex with β4

    Xiao Tao, Roderick MacKinnon
    The structures of Slo1 in complex with b4 imply that the auxiliary beta subunits modulate the channel's gating properties through stabilizing ‘pre-existing’ conformations rather than creating new ones.
    1. Neuroscience

    Amyloid and tau accumulate across distinct spatial networks and are differentially associated with brain connectivity

    Joana B Pereira, Rik Ossenkoppele ... Oskar Hansson
    The amyloid patterns overlap with the default-mode network, whereas the tau patterns overlap with distinct functional networks and are associated with a loss of anatomical connectivity and multiple cognitive functions.
    1. Developmental Biology
    2. Neuroscience

    Sequential phosphorylation of NDEL1 by the DYRK2-GSK3β complex is critical for neuronal morphogenesis

    Youngsik Woo, Soo Jeong Kim ... Sang Ki Park
    Sequential phosphorylation of NDEL1 Ser336/Ser332 by DYRK2 and GSK3β is a novel regulatory step for actin dynamics that contributes to the neurite outgrowth and neuronal arborization in the developing brain.
    1. Cell Biology
    2. Immunology and Inflammation

    B cells extract antigens at Arp2/3-generated actin foci interspersed with linear filaments

    Sophie I Roper, Laabiah Wasim ... Pavel Tolar
    Two modes of actin polymerization, branched and linear, cooperate in B cells to extract antigens from surfaces of presenting cells.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Repressive H3K9me2 protects lifespan against the transgenerational burden of COMPASS activity in C. elegans

    Teresa Wei-sy Lee, Heidi Shira David ... David John Katz
    The transgenerational inheritance of lifespan is facilitated by the accumulation of repressive chromatin across generations.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Mutations that improve efficiency of a weak-link enzyme are rare compared to adaptive mutations elsewhere in the genome

    Andrew B Morgenthaler, Wallis R Kinney ... Shelley D Copley
    Mutations elsewhere in the genome play critical roles in improving fitness during amplification and divergence of a gene encoding a weak-link enzyme whose inefficiency limits growth rate.
    1. Neuroscience

    Calpain fosters the hyperexcitability of motoneurons after spinal cord injury and leads to spasticity

    Vanessa Plantier, Irene Sanchez-Brualla ... Frédéric Brocard
    Calpain is a promising therapeutic target to reduce spasticity after a spinal cord injury.
    1. Genetics and Genomics
    2. Neuroscience

    Probe-Seq enables transcriptional profiling of specific cell types from heterogeneous tissue by RNA-based isolation

    Ryoji Amamoto, Mauricio D Garcia ... Constance L Cepko
    Probe-Seq is a cell type specific bulk RNA sequencing method that can be applied to a wide-ranging tissue types from both vertebrates and invertebrates without genetic labeling.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Two new polymorphic structures of human full-length alpha-synuclein fibrils solved by cryo-electron microscopy

    Ricardo Guerrero-Ferreira, Nicholas MI Taylor ... Henning Stahlberg
    Two new polymorphic structures of recombinant human alpha-synuclein fibrils show striking differences to previous structures, while familial PD mutation sites remain crucial for protofilament interaction and fibril stability.
    1. Immunology and Inflammation

    Tight nuclear tethering of cGAS is essential for preventing autoreactivity

    Hannah E Volkman, Stephanie Cambier ... Daniel B Stetson
    Tight nuclear tethering of the cGAS DNA sensor maintains it in its resting state and prevents activation by self-DNA.
    1. Cell Biology

    Kinetochores attached to microtubule-ends are stabilised by Astrin bound PP1 to ensure proper chromosome segregation

    Duccio Conti, Parveen Gul ... Viji M Draviam
    Human chromosome-microtubule attachments are stabilised by Astrin-mediated dynamic delivery of PP1 phosphatase to the attachment site, which ensures the normal segregation of chromosomes.
    1. Cell Biology
    2. Physics of Living Systems

    Bridging the gap between single-cell migration and collective dynamics

    Florian Thüroff, Andriy Goychuk ... Erwin Frey
    A computational model, based on single-cell features like contractility and polarizability, quantitatively describes cellular dynamics from the single cell level up to small cohorts and confluent tissues.
    1. Microbiology and Infectious Disease

    Homeostatic control of cell wall hydrolysis by the WalRK two-component signaling pathway in Bacillus subtilis

    Genevieve S Dobihal, Yannick R Brunet ... David Z Rudner
    Molecular genetic analyses define the first homeostatic control pathway that maintains cell wall remodeling activity during bacterial growth.
    1. Plant Biology

    Homeostasis of branched-chain amino acids is critical for the activity of TOR signaling in Arabidopsis

    Pengfei Cao, Sang-Jin Kim ... Federica Brandizzi
    Despite BCAAs being de novo-synthesized in chloroplasts, TOR activation by BCAAs is conserved in plants and triggers a re-organization of actin and actin-associated endomembranes.
    1. Developmental Biology

    Spatiotemporal dynamics and heterogeneity of renal lymphatics in mammalian development and cystic kidney disease

    Daniyal J Jafree, Dale Moulding ... David A Long
    Three-dimensional imaging was used to identify structural and quantitative features of developing lymphatics in the kidneys of mice, humans and in a genetic mouse model of polycystic kidney disease.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular determinants of the Ska-Ndc80 interaction and their influence on microtubule tracking and force-coupling

    Pim J Huis in 't Veld, Vladimir A Volkov ... Marileen Dogterom
    Binding of two macromolecular complexes allows kinetochores to capture force produced by the depolymerising ends of microtubules, allowing chromosomes to be transmitted from a mother cell to its two daughters.
    1. Neuroscience

    Insights into the dynamic control of breathing revealed through cell-type-specific responses to substance P

    Nathan A Baertsch, Jan-Marino Ramirez
    The neuromodulator substance P enhances mechanisms of recurrent excitation between breaths to control breathing frequency and stability.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Mechanistic basis of the inhibition of SLC11/NRAMP-mediated metal ion transport by bis-isothiourea substituted compounds

    Cristina Manatschal, Jonai Pujol-Giménez ... Raimund Dutzler
    The structural and mechanistic characterization of competitive inhibition of human DMT1 offers a promising route for the development of compounds for the treatment of iron overload disorders.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Distinct roles for S. cerevisiae H2A copies in recombination and repeat stability, with a role for H2A.1 threonine 126

    Nealia CM House, Erica J Polleys ... Catherine H Freudenreich
    Despite differing by only one amino acid in the C-terminal tail, copy 1 of yeast histone 2A has a repair-specific role not shared by copy 2.
    1. Developmental Biology
    2. Neuroscience

    Gradient-independent Wnt signaling instructs asymmetric neurite pruning in C. elegans

    Menghao Lu, Kota Mizumoto
    Genetic analyses revealed the critical role of local Wnt in neuronal patterning by instructing neurite pruning.
    1. Neuroscience

    The representational space of observed actions

    Raffaele Tucciarelli, Moritz Wurm ... Angelika Lingnau
    Representational similarity analysis of human functional magnetic resonance imaging data demonstrates that the lateral occipitotemporal cortex represents action knowledge along dimensions that are in accordance with behavioural judgements.
    1. Computational and Systems Biology
    2. Plant Biology

    Evolution of C4 photosynthesis predicted by constraint-based modelling

    Mary-Ann Blätke, Andrea Bräutigam
    Constraint-based modelling predicts C4 photosynthesis evolves under resource limitation from an ancestral ground state of C3 photosynthesis and attributes divergent metabolic routes in extant C4 subtypes to light.
    1. Biochemistry and Chemical Biology
    2. Genetics and Genomics

    Interferon-β-induced miR-1 alleviates toxic protein accumulation by controlling autophagy

    Camilla Nehammer, Patrick Ejlerskov ... Roger Pocock
    A microRNA prevents the accumulation of toxic proteins by inducing autophagy.
    1. Biochemistry and Chemical Biology

    Exchange of water for sterol underlies sterol egress from a StARkin domain

    George Khelashvili, Neha Chauhan ... Anant K Menon
    The capture and export of a hydrophobic sterol molecule from the binding pocket of a Lam/GramD1 sterol-binding protein is unexpectedly mediated by water.
    1. Cancer Biology
    2. Cell Biology

    Junction Mapper is a novel computer vision tool to decipher cell–cell contact phenotypes

    Helena Brezovjakova, Chris Tomlinson ... Vania MM Braga
    Junction Mapper is a powerful new semi-automated software that provides a fingerprint of cell–cell contact morphometry and receptor density alterations.
    1. Developmental Biology
    2. Plant Biology

    Coordination of tissue cell polarity by auxin transport and signaling

    Carla Verna, Sree Janani Ravichandran ... Enrico Scarpella
    GNOM controls both auxin transport and auxin signaling to coordinate tissue cell polarity during vein patterning in plants.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Deep sampling of Hawaiian Caenorhabditis elegans reveals high genetic diversity and admixture with global populations

    Tim A Crombie, Stefan Zdraljevic ... Erik C Andersen
    Hawaiian Caenorhabditis elegans harbor high levels of genetic diversity that might represent the complex patterns of ancestral diversity in the species prior to human influence.
    1. Neuroscience
    2. Physics of Living Systems

    Analysis of stochastic fluctuations in responsiveness is a critical step toward personalized anesthesia

    Andrew R McKinstry-Wu, Andrzej Z Wasilczuk ... Alexander Proekt
    Responses to anesthetics differ among individuals and fluctuate stochastically despite constant drug concentration, however, the amount of noise driving transitions between the responsive and the unresponsive state is conserved.
    1. Computational and Systems Biology
    2. Neuroscience

    Differences in topological progression profile among neurodegenerative diseases from imaging data

    Sara Garbarino, Marco Lorenzi ... Daniel C Alexander
    Computational-driven, imaging-based topological profiles of neurodegeneration differ substantially in different neurodegenerative conditions, suggesting distinct modes of dependence of the pathological spread on the underlying connectivity.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Dynamic regulation of chromatin accessibility by pluripotency transcription factors across the cell cycle

    Elias T Friman, Cédric Deluz ... David M Suter
    OCT4 and SOX2 display partially independent activity to regulate chromatin accessibility, and highly dynamic activity of OCT4 is required throughout the cell cycle to maintain pluripotency enhancer accessibility.
    1. Cell Biology

    Impaired lysosomal acidification triggers iron deficiency and inflammation in vivo

    King Faisal Yambire, Christine Rostosky ... Nuno Raimundo
    Impaired lysosomal acidification results in retention of iron inside lysosomes, triggering functional iron deficiency, dysfunctional mitochondria (especially mtDNA loss), and inflammation in vivo in a mouse model of lysosomal disease.
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Transmission dynamics and control of multidrug-resistant Klebsiella pneumoniae in neonates in a developing country

    Thomas Crellen, Paul Turner ... Ben S Cooper
    Colonisation with resistant Klebsiella pneumoniae in a Cambodian neonatal unit is driven by person-to-person transmission, transmissibility varies by sequence type, and antibiotic consumption generally increases the risk of acquisition.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    An integrin/MFG-E8 shuttle loads HIV-1 viral-like particles onto follicular dendritic cells in mouse lymph node

    Chung Park, John H Kehrl
    Intravital imaging with HIV-1 viral-like particle in mouse model reveals a mechanism for HIV-1 uptake by subcapsular sinus macrophages that facilitates HIV-1 spreading tofollicular dendritic and B cells.
    1. Neuroscience

    Tight junction protein occludin regulates progenitor Self-Renewal and survival in developing cortex

    Raphael M Bendriem, Shawn Singh ... M Elizabeth Ross
    That early cortical neural progenitor divisions strictly require OCLN isoform expression is a paradigm shift in the functional consideration of this protein best known for promoting between-cell barrier functions.
    1. Medicine
    2. Immunology and Inflammation

    Chronic muscle weakness and mitochondrial dysfunction in the absence of sustained atrophy in a preclinical sepsis model

    Allison M Owen, Samir P Patel ... Hiroshi Saito
    Sepsis-induced long-term muscle weakness was reproduced using a refined murine model, which was accompanied by mitochondrial dysfunction in the absence of sustained atrophy, suggesting the promise of mitochondria-targeted post-sepsis therapies.
    1. Developmental Biology
    2. Microbiology and Infectious Disease

    High fat diet induces microbiota-dependent silencing of enteroendocrine cells

    Lihua Ye, Olaf Mueller ... John F Rawls
    Enteroendocrine cells sense nutrients in the gut and regulate digestive physiology but are rendered insensitive following fat ingestion due to alteration of gut microbiota.
    1. Ecology

    Biophysical and physiological processes causing oxygen loss from coral reefs

    Cynthia B Silveira, Antoni Luque ... Forest Rohwer
    Coral reef heterotrophic bacteria and fleshy macroalgae cause oxygen loss from reef systems through microbial biomass accumulation and ebullition from algae surfaces.
    1. Genetics and Genomics
    2. Plant Biology

    The MADS-box transcription factor PHERES1 controls imprinting in the endosperm by binding to domesticated transposons

    Rita A Batista, Jordi Moreno-Romero ... Claudia Köhler
    Jumping of transposable elements provides DNA-binding sites for the MADS-box transcription factor PHERES1, allowing the regulation of imprinted genes and other key endosperm development genes.
    1. Cell Biology
    2. Neuroscience

    Neurotrophins induce fission of mitochondria along embryonic sensory axons

    Lorena Armijo-Weingart, Andrea Ketschek ... Gianluca Gallo
    Induction of the fission of mitochondria-populating sensory axons is shown to be a novel biological action of neurotrophins.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Modulating FOXO3 transcriptional activity by small, DBD-binding molecules

    Judith Hagenbuchner, Veronika Obsilova ... Michael J Ausserlechner
    The mode of binding and cellular effect of the first FOXO3-DNA-binding-domain targeting small compound discovered by a combined pharmacophore-based/biochemical screening approach is reported.
    1. Chromosomes and Gene Expression

    An mRNA-binding channel in the ES6S region of the translation 48S-PIC promotes RNA unwinding and scanning

    Irene Díaz-López, René Toribio ... Iván Ventoso
    The ES6S region of the small subunit ribosome makes a place for the threading and secondary structure unwinding of mRNA, which regulates genome-wide translation.
    1. Developmental Biology

    Coronary arterial development is regulated by a Dll4-Jag1-EphrinB2 signaling cascade

    Stanislao Igor Travisano, Vera Lucia Oliveira ... José Luis de la Pompa
    Notch ligands Jag1 and Dll4 and their effector Ephb2 are required in sinus venosus endocardium for primitive coronary vasculature formation and later for arterial differentiation and maturation of coronary endothelium.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Herpesviral lytic gene functions render the viral genome susceptible to novel editing by CRISPR/Cas9

    Hyung Suk Oh, Werner M Neuhausser ... David M Knipe
    CRISPR genome editing technology can efficiently introduce mutations into lytic and latent HSV genomes to block lytic replication and reactivation of latent herpes simplex virus genome though differential mechanisms.