Christopher I Cazzonelli, Xin Hou ... Barry J Pogson
Carotenoids are not just required as core components for plastid biogenesis, they can be cleaved into an apocarotenoid signal that regulates etioplast and chloroplast development during extended periods of darkness.
Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.
Assembly of the human enzyme inosine monophosphate dehydrogenase 2 into filaments promotes substrate-dependent resistance to feedback inhibition by downstream products, promoting increased flux during proliferative states.
Andrés Aranda-Díaz, Benjamin Obadia ... Kerwyn Casey Huang
Shifts in pH that result from metabolic interactions between members of the Drosophila gut microbiota were sufficient to modulate Lactobacillus plantarum tolerance to the antibiotics rifampin and erythromycin.
Live imaging and genetic analyses revealed that notochord vacuoles play a critical role in spine morphogenesis by absorbing vertebral bone growth, thus implicating notochord mechanics in congenital scoliosis.
Combined light and electron microscopy reveals a new function for Arp2/3-mediated actin assembly in nuclear envelope rupture, which leads to a separation of nuclear membranes and pores from the lamina.
Marion V Guerin, Veronica Finisguerra ... Alain Trautmann
Dissecting structural, cellular and molecular differences between transplanted and spontaneous mouse tumor models, highlighting their relevance for predicting the efficacy of anti-cancer treatments in patients.
Robust and wide-spread locomotion-related neural signals, revealed in the mouse auditory midbrain, suggest that integrating movement-related information is an essential aspect of midbrain sound processing.
Development of nanobodies against a model pentameric ligand-gated ion channel demonstrates they can be functionally active as negative or positive allosteric modulators and offers opportunities for future drug development.
Christopher A Jackson, Dayanne M Castro ... David Gresham
Single cell expression data can be used to determine how regulatory transcription factors and target genes are connected, and is especially useful when studying transcription factors controlling heterogeneous cell states.
Ana Filipa Domingues, Rashmi Kulkarni ... Cristina Pina
Histone acetyl-transferase Kat2a preserves leukemia stem cells through frequent transcriptional firing of metabolic and regulatory gene promoters and maintenance of a largely invariant self-renewal program.
Thomas D Miller, Trevor T-J Chong ... Clive R Rosenthal
Human hippocampal cornu ammonis 3 damage impairs both recent and remote autobiographical episodic memory, and disrupts functional integration in medial temporal lobe subsystem regions of the default network.
Madhumitha Narasimhan, Alexander Johnson ... Jiří Friml
The examination of the ultra-structure and in vivo dynamics of endocytosis in plants reveal plants unique actin-independent, clathrin-mediated endocytosis mechanisms to overcome their unique physiological properties.
The combination of statistical inference and perturbation experiments reveals that trans-generational inheritance of cell size and cell-cycle speed, coupled through a minimum-size checkpoint, shapes paradoxical cell-cycle correlations in lineage trees.
Cancer cell expression of the T-cell co-stimulatory molecule CD80, or treatment with agonistic antibodies targeting the T-cell co-stimulatory receptors OX-40 or 4-1BB, enhances the anti-tumor activity of FAK inhibition.
A peptide derived from BK and JC polyomavirus protein VP2/3 inhibits viral infection by targeting a binding site in the pore of polyomavirus VP1 pentamers, enabling future VP1-targeted therapeutic strategies.
Jennifer A Honeycutt, Camila Demaestri ... Heather C Brenhouse
Early life adversity led to hyper-innervation from the basolateral amygdala to the prefrontal cortex earlier in females than males and disrupted maturation of functional connectivity, which predicted anxiety-like outcomes.
Tatyana Bodrug, Elizabeth M Wilson-Kubalek ... Jawdat Al-Bassam
Biochemical, single molecule, cell and structural biology studies reveal an interaction between the kinesin-5 tail and motor domains regulating high-force production, which is critical for microtubule sliding motility.
Kishor K Sivaraj, Backialakshmi Dharmalingam ... Ralf H Adams
The interplay between the transcriptional co-regulators YAP1/TAZ and the hypoxia-controlled transcription factor HIF1α differentially regulates endothelial cell behavior in the hypoxic environment of bone compared to other organs.
Tobias Geiger, Maria Lara-Tejero ... Jorge E Galán
Biochemical analyses and the crystal structure of TtsA reveal fundamental insight into the mechanisms by which this muramidase recognizes its peptidoglycan substrate to facilitate typhoid toxin secretion.
An experimentally constrained multiscale mathematical model predicts that branched actin networks self-organize at endocytic sites and bend to produce force, which was verified with cryo-electron tomography of intact cells.
Jonathan M Budzik, Danielle L Swaney ... Jeffery S Cox
Measurement of post-translational modifications in primary macrophages infected with Mtb revealed phosphorylation of TAX1BP1, an autophagy receptor that enables full maturation of the Mtb autophagosome.
Katrin Kierdorf, Fabian Hersperger ... Marc S Dionne
A cytokine partly derived from macrophages is required to promote normal muscle health and metabolism by inhibiting the activity of the insulin signalling pathway in the fruitfly Drosophila melanogaster.
Vascular endothelial cells in the brain, heart and lung exhibit tissue-specific heterogeneity and plasticity, expressing genes that were traditionally thought to be only expressed by the surrounding parenchymal tissue cells.
A theoretical model comprehensively describes the stepping dynamics of the motor protein myosin V, unraveling the significance of a recently discovered structural constraint on the angle between its lever arms.
Adérito L Monjane, Simon Dellicour ... Darren P Martin
Phylogenetic analyses demonstrate an evolutionary trade-off between the amount of harm inflicted by a broad host-range virus and how effectively the virus positions itself within plants to enable onward transmission.
BUD23 links ribosome function with mitochondrial protein expression and efficient oxidative phosphorylation by promoting selective mRNA translation and is therefore critical to mouse development and postnatal cardiac function.
Sebastià Franch-Expósito, Laia Bassaganyas ... Jordi Camps
CNApp is a novel and unique web-based software that enables the performance of comprehensive and integrative analysis of genomic copy number alterations to uncover new associations with patient-oriented relevance.
Mechanical interactions between bacterial species with different motility characteristics play an important role in spatial-temporal dynamics of multi-species bacterial colonies and can lead to formation of complex patterns.
Tomomi Tsunematsu, Amisha A Patel ... Shuzo Sakata
Sub-second pontine waves functionally interact with hippocampal population activity in a state-dependent manner across sleep states, while brainstem ensemble dynamics exhibit slow, long-lasting state-predictive activity.
Both bottom-up and top-down processing are involved in the occipital-temporal face network, with the top-down modulation more extensively engaged when available information is sparse in the face images.
Renumathy Dhanasekaran, Virginie Baylot ... Dean W Felsher
MYC and Twist1 drive metastasis by a novel non-cell-autonomous transcriptional mechanism of eliciting a cytokinome that mediates the crosstalk between cancer cells and macrophages, and its therapeutic blockade inhibits metastasis.
Purification of two conserved protein complexes, the γ-TuRC and Augmin, using a simple affinity technique, demonstrates that they are necessary and sufficient for the essential phenomenon of branching microtubule nucleation.
Single molecule mRNA imaging uncovers post-transcriptional regulation of myc mRNA, via a cell-intrinsic mechanism allowing individualised control of neural stem cell proliferation during Drosophila brain development.
Rosa Maria Porreca, Emilia Herrera-Moyano ... Jean-Baptiste Vannier
TRF1 suppresses replication fork stalling, which triggers break induced replication at telomeres associated with DNA damage response, alteration in the telomere chromatin environment, and re-localisation of telomeres to PML bodies.
The Pkpk and Pksple isoforms of the Prickle protein determine right- or left-handed bristle morphogenesis by coupling Frizzled/Vang signaling to the Fat/Dachsous PCP directional signal in opposite directions.
Hua-Qian Yang, Marta Pérez-Hernández ... William A Coetzee
The functional interaction of Na+ and KATP channels at the intercalated disk of cardiomyocytes depends on Ankyrin G and is clinically relevant since KATP channel mutations affect Na+ channel expression.
In neuronal mitophagy, Parkin and OPTN induce efficient sequestration of damaged somal mitochondria into autophagosomes, but slow turnover via lysosomal acidification may be a point of vulnerability for the cell.
The transcription factor Lola is defined as a non-canonical Hippo signaling component essential for Drosophila midgut homeostasis via its interaction with Warts and suppression on Dref and SkpA expression levels.
Alejandra Escudero-Lara, Josep Argerich ... Rafael Maldonado
A mouse model of endometriosis reveals that moderate doses of Δ9-tetrahydrocannabinol produce clear regression of ectopic endometrial growths, pronounced alleviation of pelvic pain and unexpected memory improvements.
Stephanie L Tsai, Clara Baselga-Garriga, Douglas A Melton
Elucidation of the molecular basis of early wound epidermis dependence during salamander limb regeneration reveals midkine as a key modulator of wound epidermis development and wound-healing resolution.
Machine learning and experimental tests of receiver bias identify signal components critical to correct species classification in guenons, linking face pattern diversity to selection for species discrimination.
Ivana Strazic Geljic, Paola Kucan Brlic ... Tihana Lenac Rovis
Single mouse cytomegalovirus protein, m154, downmodulates surface expression of numerous targets important for NK and CD8 T cell activation by perturbing adaptor protein-1 sorting and redirecting targets to lysosomal degradation.
The structure of the COMPASS methyltransferase bound to an H2B-ubiquitinated nucleosome provides insights into mechanisms of trans-histone cross-talk and the dynamic role of H2B ubiquitination in stimulating histone methylation.
E Mae Guthman, Joshua D Garcia ... Molly M Huntsman
Sst+ interneurons drive feedforward inhibition in the basolateral amygdala, and thus provide a framework for why interneuron subtypes may mediate different archetypal circuit motifs across different brain regions.
Travis A Babola, Calvin J Kersbergen ... Dwight E Bergles
Supporting cells in the cochlea change their shape in response to purinergic receptor activation, which influences hair cell excitability by altering potassium redistribution in the extracellular space.
The transcription coactivators SAGA and TFIID have redundant function at a subset of yeast genes while SAGA also contributes to transcription of all genes through regulation of chromatin modifications.
Jong Wook Kim, Christian Berrios ... William C Hahn
ST recruitment of STRIPAK facilitates PP2A-mediated dephosphorylation of MAP4K4 and induces cell transformation highlighting that STRIPAK complex plays a key role in defining PP2A specificity and activity.
In vitro culture of brain endothelial cells leads to a rapid loss of the blood-brain barrier transcriptional and accessible chromatin landscapes that is resistant to the effects of beta-catenin stabilization.
Death receptor 5 can directly sense misfolded proteins downstream of the endoplasmic reticulum to provide a quality control mechanism that executes apoptosis and prevents further production of misfolded proteins.
Infrared laser-mediated gene induction microscopy enables precise single-cell labeling in various tissues of zebrafish, and in vivo visualized single-cell lineage tracing reveals the lineage heterogeneity in hematopoietic endothelial cells.
Harrison Tudor Evans, Liviu-Gabriel Bodea, Jürgen Götz
Quantitative de novo proteomics paired with in vivo cell-specific non-canonical amino acid labelling identified several spatial long-term memory-induced changes in protein synthesis in hippocampal neurons.
Antoine Vigouroux, Baptiste Cordier ... Sven van Teeffelen
Class-A penicillin-binding proteins are dispensable for rod-like cell-shape but essential for mechanical integrity by sensing and repairing cell-wall defects locally, as investigated in the model system Escherichia coli.
Sophia Karpenko, Sebastien Wolf ... Georges Debrégeas
Light-seeking strategies in Zebrafish larvae are dissected using a virtual-reality assay, and these data are used to establish minimal stochastic and neural-circuits models that quantitatively capture this behavior.