September 2022

Cover articles

    1. Cell Biology
    2. Genetics and Genomics

    Coping with complex I loss

    Nicholas P Lesner, Xun Wang ... Prashant Mishra
    1. Ecology
    2. Plant Biology

    Reducing competition when annual crops are grown together

    Laura Stefan, Nadine Engbersen, Christian Schöb
    1. Microbiology and Infectious Disease

    How megacytes help mosquitoes fight malaria parasites

    Ana Beatriz F Barletta, Banhisikha Saha ... Carolina Barillas-Mury
    1. Microbiology and Infectious Disease

    Megacytes versus malaria

    Ana Beatriz F Barletta, Banhisikha Saha ... Carolina Barillas-Mury

Highlights controls:

Research articles

    1. Immunology and Inflammation

    A neutrophil–B-cell axis impacts tissue damage control in a mouse model of intraabdominal bacterial infection via Cxcr4

    Riem Gawish, Barbara Maier ... Sylvia Knapp
    LPS pre-exposure is tissue protective during a subsequent lethal E. coli peritonitis by long-term modulation the bone marrow B-cell and neutrophil pool which affects neutrophil tissue damaging properties and therapeutic activation of Cxcr4 promotes tissue damage control during sepsis.
    1. Stem Cells and Regenerative Medicine

    In vivo generation of bone marrow from embryonic stem cells in interspecies chimeras

    Bingqiang Wen, Guolun Wang ... Vladimir V Kalinichenko
    Blastocyst complementation of rat embryos with donor mouse embryonic stem cells was used to simultaneously produce multiple, donor-derived hematopoietic and stromal cells in the interspecies bone marrow that exhibited normal gene expression signatures, cell surface markers, and functional characteristics.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform

    Dirk H Siepe, Lukas T Henneberg ... K Christopher Garcia
    A proteo-genomic CRISPR activation screening workflow to accelerate the identification of novel interactions between the secreted and membrane proteome with the potential to define new biological processes and identify future therapeutic targets.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Airway basal cells show regionally distinct potential to undergo metaplastic differentiation

    Yizhuo Zhou, Ying Yang ... Wellington V Cardoso
    A combination of unbiased scRNA-sequencing and functional approaches in organoid cultures and injury-repair models in vivo reveals major heterogeneity in adult murine airway basal cells, which is established prenatally and conserved in humans.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Protein evidence of unannotated ORFs in Drosophila reveals diversity in the evolution and properties of young proteins

    Eric B Zheng, Li Zhao
    The analysis of mass-spectrometry data for all possible open reading frames reveals protein evidence for evolutionarily young, unannotated proteins with distinct characters.
    1. Computational and Systems Biology

    Evolution of cell size control is canalized towards adders or sizers by cell cycle structure and selective pressures

    Felix Proulx-Giraldeau, Jan M Skotheim, Paul François
    An evolutionary algorithm is used to build gene networks implementing cell size control, and suggests multiple ways for evolution to first build sizers and turn them into adders depending on evolutionary constraints.
    1. Structural Biology and Molecular Biophysics

    The Candida albicans virulence factor candidalysin polymerizes in solution to form membrane pores and damage epithelial cells

    Charles M Russell, Katherine G Schaefer ... Francisco N Barrera
    The molecular mechanism that candidalysin uses to perforate membranes is unraveled, which opens the door to the rational design of inhibitors against candidiasis.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis

    Tianzhi Li, Qiqi Cheng ... Cong Ma
    A new binding mode between Rabphilin 3A and SNAP-25 enables efficient SNARE complex assembly via inducing a conformation change in SNAP-25 SNARE motif.
    1. Neuroscience

    Functional brain reconfiguration during sustained pain

    Jae-Joong Lee, Sungwoo Lee ... Choong-Wan Woo
    Human neuroimaging with time-evolving network analysis reveals dynamic interactions among multiple functional brain networks in response to sustained pain.
    1. Immunology and Inflammation

    Macrophage innate training induced by IL-4 and IL-13 activation enhances OXPHOS driven anti-mycobacterial responses

    Mimmi LE Lundahl, Morgane Mitermite ... Ed C Lavelle
    Type 2 cytokines induce macrophage innate training, where enhanced pro-inflammatory responses are fuelled by oxidative phosphorylation rather than aerobic glycolysis.
    1. Immunology and Inflammation

    Bronchus-associated macrophages efficiently capture and present soluble inhaled antigens and are capable of local Th2 cell activation

    Xin-Zi Tang, Lieselotte S M Kreuk ... Christopher D C Allen
    Lung interstitial macrophages are strategically positioned underneath the bronchial airway epithelium and enriched at airway bifurcations for immunosurveillance of the conducting airway lumen and the initiation of adaptive immune responses.
    1. Computational and Systems Biology
    2. Neuroscience

    Olfactory responses of Drosophila are encoded in the organization of projection neurons

    Kiri Choi, Won Kyu Kim, Changbong Hyeon
    The organization of olfactory projection neurons and their synaptic connectivity with third-order neurons studied along with olfactory responses suggest that the labeled-line design is partially at work in the Drosophila olfactory system.
    1. Neuroscience

    Putting perception into action with inverse optimal control for continuous psychophysics

    Dominik Straub, Constantin A Rothkopf
    A new method for analyzing continuous psychophysics experiments estimates not only perceptual uncertainty from tracking tasks but also action variability, intrinsic costs, and subjective internal models.
    1. Neuroscience

    Distinct population and single-neuron selectivity for executive and episodic processing in human dorsal posterior cingulate

    Lyndsey Aponik-Gremillion, Yvonne Y Chen ... Brett L Foster
    Invasive recordings from human dorsal posterior cingulate cortex show neural population responses occur for only executive tasks, while single neuron responses occur for specific executive or episodic cognitive tasks.
    1. Neuroscience

    Time encoding migrates from prefrontal cortex to dorsal striatum during learning of a self-timed response duration task

    Gabriela C Tunes, Eliezyer Fermino de Oliveira ... Marcelo Bussotti Reyes
    Electrophysiological and pharmacological data provide evidence that the medial prefrontal cortex is essential for rats to learn a timing task, becoming unnecessary later, while the dorsal striatum does not play a role initially, taking over when animals become proficient.
    1. Developmental Biology
    2. Neuroscience

    Plexins promote Hedgehog signaling through their cytoplasmic GAP activity

    Justine M Pinskey, Tyler M Hoard ... Benjamin L Allen
    The plexin family of semaphorin receptors regulate Hedgehog pathway activity via a conserved GAP domain and FYN kinase phosphorylation site in their cytoplasmic domain.
    1. Cell Biology

    De novo apical domain formation inside the Drosophila adult midgut epithelium

    Jia Chen, Daniel St Johnston
    Drosophila enteroblasts form septate junctions, an apical domain and an internal lumen inside the midgut epithelium before they reach the gut surface, thereby maintaining the intestinal barrier as they integrate.
    1. Chromosomes and Gene Expression

    The SPARC complex defines RNAPII promoters in Trypanosoma brucei

    Desislava P Staneva, Stefan Bresson ... Robin C Allshire
    The SPARC complex directs accurate RNAPII positioning for transcription initiation and, in some instances, ensures correct transcription directionality in Trypanosoma brucei.
    1. Neuroscience

    Functional gradients in the human lateral prefrontal cortex revealed by a comprehensive coordinate-based meta-analysis

    Majd Abdallah, Gaston E Zanitti ... Demian Wassermann
    A comprehensive meta-analysis of the neuroimaging literature reveals that the lateral prefrontal cortex of humans is mainly organized along its rostrocaudal axis according to a unimodal-to-transmodal pattern of network connectivity and a concrete-to-abstract axis of functional associations.
    1. Computational and Systems Biology

    Ear pinnae in a neotropical katydid (Orthoptera: Tettigoniidae) function as ultrasound guides for bat detection

    Christian A Pulver, Emine Celiker ... Fernando Montealegre-Z
    Located in the legs, the miniaturized katydid ears exhibit cuticular pinnae to only capture high-ultrasonic bat echolocation calls, but katydid also hear their own calls using alternative ear paths, which suggest that their ears operate in a colossal frequency range.
    1. Neuroscience

    Flexing the principal gradient of the cerebral cortex to suit changing semantic task demands

    Zhiyao Gao, Li Zheng ... Elizabeth Jefferies
    Gradient decomposition of informational connectivity reveals that the principal gradient with the separation of default mode network from sensory-motor systems represents a hallmark of the retrieval of strong conceptual links.
    1. Cell Biology

    Phosphoproteomic mapping reveals distinct signaling actions and activation of muscle protein synthesis by Isthmin-1

    Meng Zhao, Niels Banhos Danneskiold-Samsøe ... Katrin J Svensson
    The role of Isthmin-1 in muscle function is defined by using phosphoproteomics to identify distinct and overlapping signaling pathways between Isthmin-1 and insulin.
    1. Developmental Biology
    2. Neuroscience

    A dynamic and expandable digital 3D-atlas maker for monitoring the temporal changes in tissue growth during hindbrain morphogenesis

    Matthias Blanc, Giovanni Dalmasso ... Cristina Pujades
    The digital 3D-atlas maker will help ascribing neuronal birthdate and neuronal differentiation times upon gene disruption in zebrafish avatars for human diseases, and will allow the integration of the information generated in other laboratories.
    1. Neuroscience

    Mixed synapses reconcile violations of the size principle in zebrafish spinal cord

    Evdokia Menelaou, Sandeep Kishore, David L McLean
    In the zebrafish spinal cord, mixed synapses contribute to neuronal connectivity and resting excitability, which helps explain violations of the well-known 'size principle' of recruitment.
    1. Epidemiology and Global Health

    Trends in female-selective abortion among Asian diasporas in the United States, United Kingdom, Canada and Australia

    Catherine Meh, Prabhat Jha
    While selective abortion of female fetuses is documented within China and India, it is also significant among Asian diaspora in Canada, US, UK and Australia, particularly among Indian diaspora for second or third births following earlier girl birth(s).
    1. Epidemiology and Global Health

    The effect of calcium supplementation in people under 35 years old: A systematic review and meta-analysis of randomized controlled trials

    Yupeng Liu, Siyu Le ... Shuran Wang
    A systematic review and meta-analysis show that calcium supplementation significantly improves bone mass, implying that preventive calcium supplementation in young people (age ≤35 years) may be a shift in the window of intervention for osteoporosis.
    1. Neuroscience

    Conduction velocity along a key white matter tract is associated with autobiographical memory recall ability

    Ian A Clark, Siawoosh Mohammadi ... Eleanor A Maguire
    A new magnetic resonance brain imaging measure reveals that variations in people’s ability to recall their past experiences may be related to the speed at which electrical signals travel along axons in the parahippocampal cingulum bundle.
    1. Neuroscience

    Insulin sensitivity in mesolimbic pathways predicts and improves with weight loss in older dieters

    Lena J Tiedemann, Sebastian M Meyhöfer ... Stefanie Brassen
    Insulinergic neuromodulation of hedonic food processing predicts future weight management and improves with weight loss in older overweight dieters.
    1. Neuroscience

    Quantitative MRI reveals differences in striatal myelin in children with DLD

    Saloni Krishnan, Gabriel J Cler ... Kate E Watkins
    A new quantitative MRI protocol reveals reduced myelin in the striatum in children with developmental language disorder, shedding light on the brain basis of this disorder.
    1. Ecology

    Bumblebees retrieve only the ordinal ranking of foraging options when comparing memories obtained in distinct settings

    Cwyn Solvi, Yonghe Zhou ... Fei Peng
    Unlike humans and starlings which use memories of both absolute and relative information to decide between options in novel contexts, bumblebees rely only on the remembered ordinal ranking of options.
    1. Medicine

    Loss of full-length dystrophin expression results in major cell-autonomous abnormalities in proliferating myoblasts

    Maxime RF Gosselin, Virginie Mournetas ... Dariusz C Gorecki
    Loss of full-length dystrophin expression causes significant molecular and functional defects in human and mouse myoblast, thus closing the vicious cycle of DMD pathology.
    1. Ecology
    2. Evolutionary Biology

    Species clustering, climate effects, and introduced species in 5 million city trees across 63 US cities

    Dakota E McCoy, Benjamin Goulet-Scott ... John Kartesz
    Across 63 large US cities, street tree communities are shaped by climate, clustered by species, and made more similar between cities due to the presence of introduced species.
    1. Cell Biology
    2. Genetics and Genomics

    Resurrecting essential amino acid biosynthesis in mammalian cells

    Julie Trolle, Ross M McBee ... Harris H Wang
    Mammalian cells were engineered to synthesize valine, a metabolic capacity that had been lost from the lineage of higher eukaryotes for >500 million years.
    1. Neuroscience

    Uncertainty-based inference of a common cause for body ownership

    Marie Chancel, H Henrik Ehrsson, Wei Ji Ma
    Behavioral and computational results show that the perception of our body as our own depends on Bayesian probabilistic reasoning that take into account the variations in sensory uncertainty when integrating visual and somatosensory cues.
    1. Developmental Biology

    Integration of mouse ovary morphogenesis with developmental dynamics of the oviduct, ovarian ligaments, and rete ovarii

    Jennifer McKey, Dilara N Anbarci ... Blanche Capel
    A detailed characterization of mouse ovary development using tissue clearing and 3D lightsheet imaging identifies ovary folding, specification of cortical and medullary domains, and ovary encapsulation as major hallmarks of ovary morphogenesis.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

    Antigoni Gogolou, Celine Souilhol ... Anestis Tsakiridis
    A thorough insight into the previously unrecognised role of a critical developmental regulator known as TBXT in influencing the specification of human trunk neural crest cells, the presumed precursors of the childhood tumour neuroblastoma.
    1. Microbiology and Infectious Disease

    c-Myc plays a key role in IFN-γ-induced persistence of Chlamydia trachomatis

    Nadine Vollmuth, Lisa Schlicker ... Thomas Rudel
    The central immune modulator interferon-gamma downregulates the proto-oncogene c-Myc to shut down host cell metabolism and interfere with infection of epithelial cells by obligate intracellular pathogenic Chlamydia trachomatis.
    1. Developmental Biology
    2. Genetics and Genomics

    Fibroblast-derived Hgf controls recruitment and expansion of muscle during morphogenesis of the mammalian diaphragm

    Elizabeth M Sefton, Mirialys Gallardo ... Gabrielle Kardon
    HGF derived from fibroblasts is critical for complete muscularization of the mammalian diaphragm, and loss-of-function experiments reveal that partial muscularization of the diaphragm is not sufficient to cause congenital diaphragmatic hernias.
    1. Cell Biology
    2. Developmental Biology

    Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration

    Audrey Miller Williams, Seth Donoughe ... Sally Horne-Badovinac
    Fat2 signals from the trailing edge of each cell to localize WAVE activity to the leading edge of the cell behind, polarizing protrusive regions within cells and across the epithelium.
    1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Post-phagocytosis activation of NLRP3 inflammasome by two novel T6SS effectors

    Hadar Cohen, Noam Baram ... Motti Gerlic
    A horizontally shared type VI secretion system affects the interaction between vibrios and eukaryotic hosts.
    1. Biochemistry and Chemical Biology

    Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control

    Meiling Wu, Anda Zhao ... Dongyun Shi
    Moderate exercise upregulated compensatory antioxidant capability and reached a high-level redox balance, whereas antioxidant intervention achieved a low-level redox balance by inhibiting oxidative stress for treating diabetes.
    1. Physics of Living Systems

    Self-organized canals enable long-range directed material transport in bacterial communities

    Ye Li, Shiqi Liu ... Yilin Wu
    Mechanochemical coupling between interfacial force and biosurfactant kinetics can coordinate large-scale material transport in primitive life forms, suggesting a new principle to engineer self-organized microbial communities.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Polygenic adaptation after a sudden change in environment

    Laura Katharine Hayward, Guy Sella
    A shift in fitness optimum of a polygenic trait rapidly introduces small frequency differences between alleles with effects aligned with and opposing the shift, which gradually translate into small differences in fixation probability.
    1. Cell Biology
    2. Genetics and Genomics

    Differential requirements for mitochondrial electron transport chain components in the adult murine liver

    Nicholas P Lesner, Xun Wang ... Prashant Mishra
    Animal models reveal that mitochondrial complex I is dispensable for homeostatic functions of the mouse liver.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Wolbachia action in the sperm produces developmentally deferred chromosome segregation defects during the Drosophila mid-blastula transition

    Brandt Warecki, Simon William Abraham Titen ... William Sullivan
    Differentiation of mitotic errors occurring during late embryonic development from the well-characterized first division defects provides new insight into the mechanisms of Wolbachia-induced cytoplasmic incompatibility.
    1. Computational and Systems Biology
    2. Genetics and Genomics

    Rapid, Reference-Free human genotype imputation with denoising autoencoders

    Raquel Dias, Doug Evans ... Ali Torkamani
    A new autoencoder-based genotype imputation method shows superior accuracy across human genomes of diverse ancestry and across the allele-frequency spectrum, while delivering significantly faster inference run times relative to standard imputation tools.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation

    Edmundo G Vides, Ayan Adhikari ... Suzanne R Pfeffer
    Parkinson’s disease-associated LRRK2 kinase is recruited to membranes by its Rab GTPase substrates, and LRRK2 is both retained on membranes and further activated there by cooperative interaction with the phosphorylated Rab proteins that it generates.
    1. Cell Biology

    Consequences of PDGFRα+ fibroblast reduction in adult murine hearts

    Jill T Kuwabara, Akitoshi Hara ... Michelle D Tallquist
    Cardiac fibroblast reduction is well-tolerated and demonstrates beneficial outcomes after heart injury.
    1. Neuroscience

    Non-rapid eye movement sleep determines resilience to social stress

    Brittany J Bush, Caroline Donnay ... J Christopher Ehlen
    Pre-existing variability in the regulation of non-rapid eye movement sleep predicts resilience to social-defeat stress.
    1. Developmental Biology

    Regulation of Nodal signaling propagation by receptor interactions and positive feedback

    Hannes Preiß, Anna C Kögler ... Patrick Müller
    A subset of the zebrafish Nodal receptor complex is regulated by positive feedback and controls the diffusion and distribution of Nodal signals during germ layer formation.
    1. Ecology
    2. Evolutionary Biology

    Mountain gorillas maintain strong affiliative biases for maternal siblings despite high male reproductive skew and extensive exposure to paternal kin

    Nicholas M Grebe, Jean Paul Hirwa ... Stacy Rosenbaum
    Mountain gorillas, who live in close-knit social groups with siblings and non-siblings of both sexes throughout their lives, show distinct behavioral biases towards maternal versus paternal kin.
    1. Epidemiology and Global Health

    SARS-CoV-2 antibody dynamics in blood donors and COVID-19 epidemiology in eight Brazilian state capitals: A serial cross-sectional study

    Carlos A Prete Jr, Lewis F Buss ... Ester C Sabino
    Blood donor serosurveillance in eight of Brazil’s most populous cities reveals extensive variation of SARS-CoV-2 attack rate across cities, age, and sex groups in December 2020 and increased intrinsic severity of the Gamma variant of concern.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations

    Anna M Langmüller, Jackson Champer ... Philipp W Messer
    In Drosophila, genomic CRISPR/Cas9 expression necessary for CRISPR-based gene drives harbors fitness costs presumably caused by off-target effects, which can be minimized by using a high-fidelity version of Cas9.
    1. Developmental Biology
    2. Genetics and Genomics

    Variable paralog expression underlies phenotype variation

    Raisa Bailon-Zambrano, Juliana Sucharov ... James T Nichols
    Within populations, there is standing variation in paralog expression levels, paralog expression levels are heritable, and variation in paralog expression can modify the phenotypes associated with mutation of one of the paralogs.
    1. Neuroscience

    A dopamine-gated learning circuit underpins reproductive state-dependent odor preference in Drosophila females

    Ariane C Boehm, Anja B Friedrich ... Ilona C Grunwald Kadow
    Behavioral and in vivo imaging analysis in the fly suggests that mating-related sensory experience regulates female odor perception and expression of choice behavior through a dopamine-gated learning circuit.
    1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    Contribution of Trp63CreERT2-labeled cells to alveolar regeneration is independent of tuft cells

    Huachao Huang, Yinshan Fang ... Jianwen Que
    A population of p63+ Krt5- epithelial cells contribute to alveolar regeneration independent of the presence of ectopic tuft cells following viral infection.
    1. Cancer Biology
    2. Computational and Systems Biology

    Proteogenomic analysis of cancer aneuploidy and normal tissues reveals divergent modes of gene regulation across cellular pathways

    Pan Cheng, Xin Zhao ... Teresa Davoli
    RNA-level regulation of gene expression is negatively associated with protein-level regulation across cellular pathways in normal tissues and cancer.
    1. Neuroscience

    Higher-order unimodal olfactory sensory preconditioning in Drosophila

    Juan Martinez-Cervantes, Prachi Shah ... Isaac Cervantes-Sandoval
    Drosophila flies infer value to olfactory stimuli based on the previously repeatedly presented associative structure between two odorants.
    1. Immunology and Inflammation
    2. Medicine

    Bone marrow adipocytes drive the development of tissue invasive Ly6Chigh monocytes during obesity

    Parastoo Boroumand, David C Prescott ... Amira Klip
    Diet-induced obesity in mice led to a duration of diet-dependent bone marrow adipocyte whitening, which drove monocyte expansion and skewing towards a tissue-invasive phenotype, likely contributing to ensuing tissue infiltration that accompanies obesity and diabetes.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    H3K9me1/2 methylation limits the lifespan of daf-2 mutants in C. elegans

    Meng Huang, Minjie Hong ... Xuezhu Feng
    The depletion of a new class of putative histone methyltransferases, including MET-2, SET-6, SET-19, SET-20, SET-21, SET-32, and SET-33, induced synergistic lifespan extension in daf-2 animals to an average lifespan nearly three times that of wild-type animals.
    1. Neuroscience

    Repressor element 1-silencing transcription factor deficiency yields profound hearing loss through Kv7.4 channel upsurge in auditory neurons and hair cells

    Haiwei Zhang, Hongchen Li ... Ping Lv
    In the cochlea, repressor element 1-silencing transcription factor (REST) regulates the expression levels of Kv7.4 and alterations of REST functions result in progressive hearing loss.
    1. Cancer Biology
    2. Chromosomes and Gene Expression

    DNA methylome combined with chromosome cluster-oriented analysis provides an early signature for cutaneous melanoma aggressiveness

    Arnaud Carrier, Cécile Desjobert ... Paola B Arimondo
    An original molecular multi-step approach integrating DNA methylome and chromosome-cluster localization cross-analysis identified a DNA methylation signature of patient outcome supporting that the core DNA methylation for a given trait is independent of the physiological context under which it arises.
    1. Immunology and Inflammation
    2. Physics of Living Systems

    Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generation

    Derek M Britain, Jason P Town, Orion David Weiner
    A light-controllable ligand is used to probe where in the signaling cascade T cells discriminate self from non-self.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Group II truncated haemoglobin YjbI prevents reactive oxygen species-induced protein aggregation in Bacillus subtilis

    Takeshi Imai, Ryuta Tobe ... Hisaaki Mihara
    The repair of oxidatively damaged proteins by the newly discovered activity in YjbI is important for the adaptation of Bacillus subtilis to oxidative environments.
    1. Developmental Biology
    2. Genetics and Genomics

    Diverse ancestry whole-genome sequencing association study identifies TBX5 and PTK7 as susceptibility genes for posterior urethral valves

    Melanie MY Chan, Omid Sadeghi-Alavijeh ... Daniel P Gale
    A genome-wide association study using whole-genome sequencing data identifies the first known susceptibility genes for posterior urethral valves, the most common cause of kidney failure in boys, implicating the transcription factor TBX5 and planar cell polarity gene PTK7.
    1. Cancer Biology
    2. Immunology and Inflammation

    Transient cell-in-cell formation underlies tumor relapse and resistance to immunotherapy

    Amit Gutwillig, Nadine Santana-Magal ... Yaron Carmi
    Tumors escape killing by the immune system through generating transient spatial cell-in-cell structures that are impenetrable to cytotoxic compounds including lytic granules and chemotherapy.
    1. Neuroscience

    Distance estimation from monocular cues in an ethological visuomotor task

    Philip RL Parker, Elliott TT Abe ... Cristopher M Niell
    Mice accurately perform an ethological distance estimation task based on gap jumping and can use monocular cues to estimate distance in addition to binocular cues such as stereopsis.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Global chromatin mobility induced by a DSB is dictated by chromosomal conformation and defines the HR outcome

    Fabiola García Fernández, Etienne Almayrac ... Emmanuelle Fabre
    A designed system to track homologous recombination (HR) in vivo demonstrates the importance of chromosome organization in the induction of global mobility in response to double-strand breaks and characterizes the role of two types of global motility in HR.
    1. Computational and Systems Biology

    Network design principle for robust oscillatory behaviors with respect to biological noise

    Lingxia Qiao, Zhi-Bo Zhang ... Lei Zhang
    Searching all two- and three-node gene regulatory network topologies identifies the repressilator with positive autoregulation as the motif capable of accurate oscillation and reveals the long period and high amplitude as two distinct mechanisms for buffering different noise.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Kinesin-1, -2, and -3 motors use family-specific mechanochemical strategies to effectively compete with dynein during bidirectional transport

    Allison M Gicking, Tzu-Chen Ma ... William O Hancock
    The motility of three different kinesin-dynein pairs were analyzed and found to be strikingly similar, suggesting that bidirectional transport is not regulated by motor type, but rather by scaffold, adapter, and regulatory proteins.
    1. Neuroscience

    Frequency-specific neural signatures of perceptual content and perceptual stability

    Richard Hardstone, Matthew W Flounders ... Biyu J He
    While perceptual content is encoded in non-oscillatory activity in the slow cortical potential (<5 Hz) range, perceptual stability is predominantly influenced by the amplitude fluctuations of alpha (~10 Hz) and beta (~20 Hz) brain oscillations.
    1. Medicine

    FSH-blocking therapeutic for osteoporosis

    Sakshi Gera, Tan-Chun Kuo ... Mone Zaidi
    The first-in-class, humanized, epitope-specific FSH-blocking antibody MS-Hu6 is efficacious, durable, and manufacturable, and is therefore poised for further development towards future testing in human trials for osteoporosis, obesity, dyslipidemia and neurodegeneration.
    1. Neuroscience

    Stability of motor representations after paralysis

    Charles Guan, Tyson Aflalo ... Richard A Andersen
    Neural populations in PPC dynamically represent motor-like and then sensory-like aspects of brain–computer interface finger movements with a representational structure that matches able-bodied individuals.
    1. Computational and Systems Biology

    Empirical single-cell tracking and cell-fate simulation reveal dual roles of p53 in tumor suppression

    Ann Rancourt, Sachiko Sato, Masahiko S Satoh
    Single-cell tracking and cell-fate simulation suggest that low levels of p53 suppress aneuploid cell formation in unstressed cells and also reveal the dual fate of cells lacking p53 in the presence of p53-proficient cells.
    1. Epidemiology and Global Health
    2. Medicine

    The ellipse of insignificance, a refined fragility index for ascertaining robustness of results in dichotomous outcome trials

    David Robert Grimes
    An ellipse of insignificant analysis is a robust method for ascertaining the strength of even large dichotomous outcome trials in biomedical science, and is a novel means to detect potentially dubious results and research fraud.
    1. Neuroscience

    7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome

    Hideaki Tomita, Kelly M Hines ... Libin Xu
    A 7-dehydrocholesterol-derived oxysterol in SLOS was found to profoundly impact neurogenesis during cortical development by interacting with glucocorticoid receptor, which points to new therapeutic approaches for SLOS by targeting the activities of this oxysterol.
    1. Developmental Biology
    2. Neuroscience

    Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons

    Jennifer M Lin, Tyler A Mitchell ... Paolo Emanuele Forni
    In vivo genetic experiments show that aberrant expression of a transcription factor can compromise the functionality of neurons which is important to better understand the potential mechanisms underlying neuropathologies.
    1. Neuroscience

    A partially nested cortical hierarchy of neural states underlies event segmentation in the human brain

    Linda Geerligs, Dora Gözükara ... Umut Güçlü
    The ongoing stream of information that comes in through our senses is segmented into distinct neural states at each level of the cortical hierarchy, which underpins our experience of distinct events.
    1. Cancer Biology
    2. Medicine

    Photonic hyperthermia of malignant peripheral nerve sheath tumors at the third near-infrared biowindow

    Yihui Gu, Zhichao Wang ... Qingfeng Li
    In vivo and in vitro experiments confirm the conspicuous antineoplastic efficacy of NIR-III photothermal therapy under the premise of ensuring biosafety.
    1. Epidemiology and Global Health

    Linking rattiness, geography and environmental degradation to spillover Leptospira infections in marginalised urban settings: An eco-epidemiological community-based cohort study in Brazil

    Max T Eyre, Fábio N Souza ... Federico Costa
    While flooding events may drive spillover transmission of Leptospira spp. through dispersal of the bacteria at low elevations, at higher elevations environmental risk is more localised and directly driven by the distribution of local rat reservoir populations.
    1. Neuroscience

    Neural signatures of auditory hypersensitivity following acoustic trauma

    Matthew McGill, Ariel E Hight ... Daniel B Polley
    Chronic two-photon calcium imaging, optogenetics, and operant behavioral approaches were leveraged to show that hyperactivity, hyperresponsivity, and hypersynchronization in auditory cortex neural ensembles can account for auditory hypersensitivity following acoustic trauma.
    1. Cell Biology
    2. Immunology and Inflammation

    Microtubules restrict F-actin polymerization to the immune synapse via GEF-H1 to maintain polarity in lymphocytes

    Judith Pineau, Léa Pinon ... Paolo Pierobon
    Microfluidics and dynamic imaging allow systematic characterization of polarization kinetics in B lymphocytes and highlight the role of microtubules as master regulators of actin polymerization at the immune synapse.
    1. Microbiology and Infectious Disease

    Mobilome-driven segregation of the resistome in biological wastewater treatment

    Laura de Nies, Susheel Bhanu Busi ... Paul Wilmes
    Biological wastewater treatment plants are critical reservoirs of antimicrobial resistance (AMR), allowing for early detection and monitoring of resistant pathogens, whilst serving as models for understanding the segregation of mobile genetic elements through AMR.
    1. Immunology and Inflammation
    2. Medicine

    Neutrophil-mediated fibroblast-tumor cell il-6/stat-3 signaling underlies the association between neutrophil-to-lymphocyte ratio dynamics and chemotherapy response in localized pancreatic cancer: A hybrid clinical-preclinical study

    Iago de Castro Silva, Anna Bianchi ... Jashodeep Datta
    A neutrophil-CAF-tumor cell IL-1β/IL-6/STAT-3 signaling axis in the pancreatic tumor microenvironment underlies the association between neutrophil-to-lymphocyte ratio dynamics and pathologic response in patients with pancreatic ductal adenocarcinoma undergoing neoadjuvant therapy.
    1. Developmental Biology

    Murine blastocysts generated by in vitro fertilization show increased Warburg metabolism and altered lactate production

    Seok Hee Lee, Xiaowei Liu ... Paolo F Rinaudo
    In vitro fertilization-conceived murine embryos show evidence of oxidative and metabolic stress with alteration in lactic acid metabolism.
    1. Neuroscience

    Shared mechanisms of auditory and non-auditory vocal learning in the songbird brain

    James N McGregor, Abigail L Grassler ... Samuel J Sober
    A novel, non-auditory learning paradigm reveals that songbirds can modify their vocal output based on somatosensory signals and that a common set of brain pathways underlies both this form of vocal learning and auditory-guided vocal learning.
    1. Developmental Biology

    A neurogenic signature involving monoamine Oxidase-A controls human thermogenic adipose tissue development

    Javier Solivan-Rivera, Zinger Yang Loureiro ... Silvia Corvera
    A hybrid mouse/human model using mesenchymal progenitor cells reveals dynamics of human adipose tissue development and mechanisms that may enhance human adipose thermogenic capacity.
    1. Immunology and Inflammation

    Memory persistence and differentiation into antibody-secreting cells accompanied by positive selection in longitudinal BCR repertoires

    Artem Mikelov, Evgeniia I Alekseeva ... Ivan V Zvyagin
    High degree of clonal persistence and excess of inter-individual convergence are observed in human memory B cell repertoires, along with signatures of both negative and positive selection in most abundant clonal lineages.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation

    Yang S Chen, Wanfu Hou ... Brian M Zid
    A new mechanism of Rvb1/Rvb2 proteins was found that couples transcription, mRNA granular localization and translation of select genes during glucose starvation stress.
    1. Biochemistry and Chemical Biology
    2. Cancer Biology

    Solute exchange through gap junctions lessens the adverse effects of inactivating mutations in metabolite-handling genes

    Stefania Monterisi, Johanna Michl ... Pawel Swietach
    Imaging studies of connexin-coupled networks of colorectal cancer cells reveal that cells carrying a genetic defect in an essential metabolic pathway can be rescued by diffusive exchange with neighboring wild-type cells via gap junctions, particularly Cx26, thereby evading negative selection.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission

    Dawn M Wenzel, Douglas R Mackay ... Wesley I Sundquist
    A comprehensive, quantitative, protein-protein interaction screen maps the network of interactions between human MIT domains and ESCRT-III C-terminal tails, and reveals MIT domain-containing protein cofactors for abscission and the 'No Cut' abscission checkpoint.
    1. Computational and Systems Biology
    2. Neuroscience

    Reverse engineering of metacognition

    Matthias Guggenmos
    A model framework and toolbox to quantify metacognitive biases and sources of metacognitive noise in animal and human confidence data.
    1. Neuroscience

    3D optogenetic control of arteriole diameter in vivo

    Philip J O'Herron, David A Hartmann ... Andy Y Shih
    The diameter of individual or multiple arterioles can be rapidly controlled with high spatiotemporal resolution using optogenetics, providing a technique for the direct manipulation of blood flow independently of neural activity or pharmacological agents.
    1. Neuroscience

    Balance between breadth and depth in human many-alternative decisions

    Alice Vidal, Salvador Soto-Faraco, Rubén Moreno-Bote
    A novel experimental paradigm investigating how humans manage limited search capacity over many alternatives reveals close-to-optimal behaviours and identifies heuristics used to minimize computation with little impact on performance.
    1. Cancer Biology
    2. Cell Biology

    GCN2 eIF2 kinase promotes prostate cancer by maintaining amino acid homeostasis

    Ricardo A Cordova, Jagannath Misra ... Kirk A Staschke
    The ISR kinase GCN2 is critical for maintaining tumor amino acid levels to facilitate growth, suggesting a novel therapeutic strategy for the treatment of prostate cancer by inducing starvation for essential amino acids.
    1. Neuroscience

    Learning of probabilistic punishment as a model of anxiety produces changes in action but not punisher encoding in the dmPFC and VTA

    David S Jacobs, Madeleine C Allen ... Bita Moghaddam
    A novel approach to studying anxiety reveals that learned anxiety and diazepam treatment have unique roles on prefrontal cortex and ventral tegmental area activity.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway

    Tolulope Sokoya, Jan Parolek ... Joost CM Holthuis
    Organellar lipidomics and lipid reporter studies in intact cells reveal how disease-relevant mutations in a sphingolipid biosynthetic enzyme cause a wide-ranging perturbation of lipid distributions and membrane properties along the secretory pathway.
    1. Ecology

    Experimental evidence that chronic outgroup conflict reduces reproductive success in a cooperatively breeding fish

    Ines Braga Goncalves, Andrew N Radford
    Outgroup conflict can negatively impact general reproductive behaviour, egg investment, and parental care, resulting in decreased reproductive output even in the absence of physical confrontations.
    1. Evolutionary Biology

    Isometric spiracular scaling in scarab beetles—implications for diffusive and advective oxygen transport

    Julian M Wagner, C Jaco Klok ... Jon F Harrison
    Diffusion but not advection becomes increasingly challenging for gas transport across insect spiracles as body size increases.
    1. Neuroscience

    Neural effects of continuous theta-burst stimulation in macaque parietal neurons

    Maria C Romero, Lara Merken ... Marco Davare
    Electrophysiological recordings in awake behaving monkeys show the first evidence of the effect of continuous theta-burst stimulation, a widely used repetitive transcranial magnetic stimulation protocol, at the level of single neurons.
    1. Structural Biology and Molecular Biophysics

    Mars, a molecule archive suite for reproducible analysis and reporting of single-molecule properties from bioimages

    Nadia M Huisjes, Thomas M Retzer ... Karl E Duderstadt
    Mars provides a software platform for the development of complex single-molecule analysis workflows enabling the discovery and reporting of new biological phenomena with an open data format accessible to everyone.
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Regional importation and asymmetric within-country spread of SARS-CoV-2 variants of concern in the Netherlands

    Alvin X Han, Eva Kozanli ... Chantal Reusken
    Flight restrictions targeted at countries where SARS-CoV-2 variant-of-concern first emerged have limited effectiveness in deterring their introduction into the Netherlands due to the strength of regional travel importation risks in Europe.
    1. Neuroscience

    The role of anterior insular cortex inputs to dorsolateral striatum in binge alcohol drinking

    David L Haggerty, Braulio Munoz ... Brady K Atwood
    A novel brain circuit that determines how much alcohol a mouse binge drinks by complex control of drinking behavior mechanics is surveyed.
    1. Neuroscience

    Controllability boosts neural and cognitive signatures of changes-of-mind in uncertain environments

    Marion Rouault, Aurélien Weiss ... Valentin Wyart
    Task controllability manipulations reveal that information seeking is associated with reduced confidence and active hypothesis testing, as well as stronger neurophysiological correlates of attention and arousal.
    1. Developmental Biology

    Innexin function dictates the spatial relationship between distal somatic cells in the Caenorhabditis elegans gonad without impacting the germline stem cell pool

    Theadora Tolkin, Ariz Mohammad ... David Greenstein
    Somatic gonad architecture is regulated by innexin function, but sheath cell position does not determine germ cell exit from the stem cell pool.
    1. Microbiology and Infectious Disease

    Genetic and chemical validation of Plasmodium falciparum aminopeptidase PfA-M17 as a drug target in the hemoglobin digestion pathway

    Rebecca CS Edgar, Ghizal Siddiqui ... Tania F de Koning-Ward
    Plasmodium falciparum aminopeptidase PfA-M17 is essential to parasite survival and plays a role in hemoglobin digestion, providing a rationale for further development of inhibitors against this enzyme.
    1. Computational and Systems Biology
    2. Evolutionary Biology

    Eco-evolutionary dynamics of clonal multicellular life cycles

    Vanessa Ress, Arne Traulsen, Yuriy Pichugin
    The evolution of clonal multicellular life cycles, whose growth is constrained by competition, may lead to coexistence or multistability between several life cycles while evolutionarily stable strategies can be inferred from the analysis of a model with unconstrained growth.
    1. Cell Biology
    2. Computational and Systems Biology

    A unified view of low complexity regions (LCRs) across species

    Byron Lee, Nima Jaberi-Lashkari, Eliezer Calo
    Disparate functions of low complexity regions of proteins can be understood through a global view of their sequences, features, and relationships across different organisms and biological contexts.
    1. Medicine

    A global view of the aspiring physician-scientist

    Christopher S Williams, W Kimryn Rathmell ... Mone Zaidi
    Challenges faced by the declining global workforce of young physician-scientists are highlighted, and a unified view on how to revive and reinvigorate this critical pool of dual-trained physicians worldwide is provided.
    1. Ecology
    2. Plant Biology

    Rapid transgenerational adaptation in response to intercropping reduces competition

    Laura Stefan, Nadine Engbersen, Christian Schöb
    Annual crop communities are able to adapt towards reduced competition and/or increased facilitation in response to their neighboring diversity after only two generations.
    1. Cancer Biology

    BRCA2 BRC missense variants disrupt RAD51-dependent DNA repair

    Judit Jimenez-Sainz, Joshua Mathew ... Ryan B Jensen
    Novel variants in the BRC repeat region of BRCA2 were identified and comprehensively characterized as either deleterious or benign.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Structure of the HOPS tethering complex, a lysosomal membrane fusion machinery

    Dmitry Shvarev, Jannis Schoppe ... Christian Ungermann
    The structure of the yeast HOPS tethering complex suggests how this large complex may catalyze fusion by tethering Rab-decorated membranes and promoting the assembly of SNAREs.
    1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Effectiveness of rapid SARS-CoV-2 genome sequencing in supporting infection control for hospital-onset COVID-19 infection: Multicentre, prospective study

    Oliver Stirrup, James Blackstone ... Judith Breuer
    Sequencing of viral genomes could be useful for infection prevention and control within hospitals, but did not lead to a reduction in the rate of hospital-acquired SARS-CoV-2 infections over winter 2020/2021 in a large multicentre UK study.
    1. Neuroscience

    Robust group- but limited individual-level (longitudinal) reliability and insights into cross-phases response prediction of conditioned fear

    Maren Klingelhöfer-Jens, Mana R Ehlers ... Tina B Lonsdorf
    Reliability and predictability analyses beyond standard measures provide empirically based guidance regarding the design of fear conditioning tasks to assess individual differences and group-level inferences cross-sectionally and longitudinally.
    1. Developmental Biology
    2. Neuroscience

    Differentiation signals from glia are fine-tuned to set neuronal numbers during development

    Anadika R Prasad, Inês Lago-Baldaia ... Vilaiwan M Fernandes
    Extrinsic signals establish an invariant and stereotyped pattern of neuronal differentiation and programmed cell death in the Drosophila visual system.
    1. Cell Biology
    2. Developmental Biology

    Live-cell imaging in human colonic monolayers reveals ERK waves limit the stem cell compartment to maintain epithelial homeostasis

    Kelvin W Pond, Julia M Morris ... Andrew L Paek
    Human colonic organoid monolayers self organize into regularly spaced stem and differentiated cell compartments, which are maintained by waves of ERK activity originating from extruded/dying cells.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    The C. elegans gonadal sheath Sh1 cells extend asymmetrically over a differentiating germ cell population in the proliferative zone

    Xin Li, Noor Singh ... Kacy Lynn Gordon
    As Caenorhabditis elegans adult hermaphrodite germ cells leave the stem cell niche, they associate with a pair of somatic gonad cells that were thought to be symmetrical, but that actually take on dramatically different positions in the organ.
    1. Microbiology and Infectious Disease

    The acid ceramidase/ceramide axis controls parasitemia in Plasmodium yoelii-infected mice by regulating erythropoiesis

    Anne Günther, Matthias Hose ... Wiebke Hansen
    Host cell acid ceramidase activity contributes to the regulation of erythrocyte maturation and thereby affects the frequency of target cells for rodent Plasmodium yoelii parasites.
    1. Cell Biology

    The number of cytokinesis nodes in mitotic fission yeast scales with cell size

    Wasim A Sayyad, Thomas D Pollard
    Fission yeasts cells have about 190 cytokinesis nodes in a broad band at the equator which scales with cell size and most but not all nodes condense into the contractile ring.
    1. Biochemistry and Chemical Biology

    DNA passes through cohesin’s hinge as well as its Smc3–kleisin interface

    James E Collier, Kim A Nasmyth
    By identifying cohesin's DNA entry gate(s) and through understanding the corresponding topology between the two the mechanism behind cohesin's activity is slowly deciphered and will aid in understanding how the SMC family at large functions.
    1. Neuroscience

    Neural synchronization is strongest to the spectral flux of slow music and depends on familiarity and beat salience

    Kristin Weineck, Olivia Xin Wen, Molly J Henry
    Two different analysis approaches for measuring neural synchronization to natural music revealed strongest synchronization to musical spectral flux as opposed to the more commonly used amplitude envelope.
    1. Neuroscience

    Time-resolved parameterization of aperiodic and periodic brain activity

    Luc Edward Wilson, Jason da Silva Castanheira, Sylvain Baillet
    The new method and reported findings address a growing interest in neuroscience for research tools that can reliably decompose brain activity at the mesoscopic scale into interpretable components.
    1. Structural Biology and Molecular Biophysics

    Unfolding and identification of membrane proteins in situ

    Nicola Galvanetto, Zhongjie Ye ... Vincent Torre
    The isolation of the cell membrane of single cells coupled with AFM enables the study and identification of native membrane proteins in a membrane fragment.
    1. Cancer Biology

    Arbidol inhibits human esophageal squamous cell carcinoma growth in vitro and in vivo through suppressing ataxia telangiectasia and Rad3-related protein kinase

    Ning Yang, Xuebo Lu ... Kangdong Liu
    Arbidol is a ATR inhibitor to reduce the phosphorylation and activation of MCM2 at Ser108, and innhibits the cell proliferation of ESCC in vitro and in vivo through the DNA replication and cell cycle pathway.
    1. Computational and Systems Biology
    2. Neuroscience

    Automatically tracking feeding behavior in populations of foraging C. elegans

    Elsa Bonnard, Jun Liu ... Monika Scholz
    A new tool enables measuring feeding and locomotion simultaneously which will enable insights into environmental, developmental, neuronal, and genetic factors underlying behavioral regulation.
    1. Evolutionary Biology
    2. Genetics and Genomics

    GENESPACE tracks regions of interest and gene copy number variation across multiple genomes

    John T Lovell, Avinash Sreedasyam ... Jeremy Schmutz
    GENESPACE lets users track related chromosomal sequences across multiple reference genomes.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Redox-controlled reorganization and flavin strain within the ribonucleotide reductase R2b–NrdI complex monitored by serial femtosecond crystallography

    Juliane John, Oskar Aurelius ... Martin Högbom
    The flavoprotein NrdI in class Ib ribonucleotide reductase controls superoxide generation and metal site oxidation by tuning the redox properties of its flavin cofactor via steric strain induced by the formation of the R2b–NrdI protein complex.
    1. Developmental Biology

    High hedgehog signaling is transduced by a multikinase-dependent switch controlling the apico-basal distribution of the GPCR smoothened

    Marina Gonçalves Antunes, Matthieu Sanial ... Isabelle Becam
    Hedgehog leads to Smoothened accumulation by favoring its recycling and high Hedgehog levels promote a basolateral enrichment of Smoothened via its phosphorylation by the protein kinase Fused.
    1. Neuroscience

    Foveal vision anticipates defining features of eye movement targets

    Lisa M Kroell, Martin Rolfs
    Before a saccadic eye movement to a location in the visual periphery, human observers' foveal vision becomes more sensitive to the features defining the eye movement target, anticipating information that will soon be fixated in a retinotopic reference frame.
    1. Genetics and Genomics

    Integrative analysis of metabolite GWAS illuminates the molecular basis of pleiotropy and genetic correlation

    Courtney J Smith, Nasa Sinnott-Armstrong ... Jonathan K Pritchard
    Genetic variants acting between biologically related metabolite pairs often reflect biology local to the traits and have effect directions contrasting those of upstream or downstream variants, which can result in striking, biologically interpretable heterogeneity in genetic correlation across the genome.
    1. Microbiology and Infectious Disease

    Distinct regions of H. pylori’s bactofilin CcmA regulate protein–protein interactions to control helical cell shape

    Sophie R Sichel, Benjamin P Bratton, Nina R Salama
    The helical cell shape of Helicobacter pylori depends on the polymerizing cytoskeletal protein CcmA’s recruitment to the cell envelope by Csd5 and CcmA’s indirect stabilization of a periplasmic cell wall hydrolase via interactions with the transmembrane protein Csd7.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    Fip1 is a multivalent interaction scaffold for processing factors in human mRNA 3′ end biogenesis

    Lena Maria Muckenfuss, Anabel Carmen Migenda Herranz ... Martin Jinek
    Molecular details of the interactions of human Fip1 with CPSF30 and CstF77 unravel key aspects of mRNA polyadenylation and its regulation.
    1. Cancer Biology
    2. Immunology and Inflammation

    Cancer genome and tumor microenvironment: Reciprocal crosstalk shapes lung cancer plasticity

    Siavash Mansouri, Daniel Heylmann ... Rajkumar Savai
    The crosstalk between cancer cell genomic features and the tumor microenvironment (TME) to reveal the impact of genetic alterations on the TME phenotype.
    1. Cell Biology

    Regulation of pulmonary surfactant by the adhesion GPCR GPR116/ADGRF5 requires a tethered agonist-mediated activation mechanism

    James P Bridges, Caterina Safina ... Marie-Gabrielle Ludwig
    Mutagenesis and activity assays in vitro and in vivo identify residues in the tethered agonist of GPR116, as well as those within the extracellular loops, that are critical for mediating the response to the tethered agonist.
    1. Neuroscience

    Evidence accumulation, not ‘self-control’, explains dorsolateral prefrontal activation during normative choice

    Cendri A Hutcherson, Anita Tusche
    A computational model of decision making suggests that the dorsolateral prefrontal cortex's role in self-control is more associated with evidence accumulation processes than with inhibition or modulation of value.
    1. Neuroscience

    Low and high frequency intracranial neural signals match in the human associative cortex

    Corentin Jacques, Jacques Jonas ... Bruno Rossion
    Category-selective intracerebral neurophysiological activity in low- and high-frequency bands show unprecedented corresponding spatial, functional, and timing properties in the human brain.
    1. Stem Cells and Regenerative Medicine

    Injury-induced pulmonary tuft cells are heterogenous, arise independent of key Type 2 cytokines, and are dispensable for dysplastic repair

    Justinn Barr, Maria Elena Gentile ... Andrew E Vaughan
    Influenza-induced pulmonary tuft cells are a heterogenous population and emerge independently of Type 2 and interferon signaling, and do not impact dysplastic epithelial regeneration.
    1. Computational and Systems Biology
    2. Plant Biology

    Computational modeling and quantitative physiology reveal central parameters for brassinosteroid-regulated early cell physiological processes linked to elongation growth of the Arabidopsis root

    Ruth Großeholz, Friederike Wanke ... Klaus Harter
    The recurring application of computational modelling combined with wet lab experiments identify and predict central regulatory parameters and factors for early cell physiological reactions linked to differential brassinosteroid-modified elongation growth in the root tip of Arabidopsis thaliana.
    1. Immunology and Inflammation

    Epigenetic remodeling by vitamin C potentiates plasma cell differentiation

    Heng-Yi Chen, Ana Almonte-Loya ... Chan-Wang Jerry Lio
    Vitamin C supports the differentiation of antibody-secreting cells by remodeling the DNA methylome to enhance STAT3 DNA binding.
    1. Cell Biology
    2. Ecology

    Ecological lipidology

    Laura Christin Trautenberg, Marko Brankatschk ... Klaus Reinhardt
    Effects of dietary lipids on organismal function and reproductive fitness are persistent and may depend on lipid identity, cause the evolution of lipid-based diet choice, alter food webs, change species responses to environmental change, and affect human nutrition.
    1. Neuroscience

    Impact of blindness onset on the representation of sound categories in occipital and temporal cortices

    Stefania Mattioni, Mohamed Rezk ... Olivier Collignon
    Early and late visual deprivation trigger a redeployment mechanism that reallocate part of the processing typically tagging the preserved senses (i.e. the temporal cortex for auditory stimulation) to the occipital cortex deprived of its most salient visual input.
    1. Cancer Biology

    Genetically manipulating endogenous Kras levels and oncogenic mutations in vivo influences tissue patterning of murine tumorigenesis

    Özgün Le Roux, Nicole LK Pershing ... Christopher M Counter
    Altering the level of different oncogenic mutants of Kras induces unique tumor patterns in mice, suggesting that tissue-specific responses mold the sensitivity of normal tissues to different oncogenic RAS mutations.
    1. Neuroscience

    Altered regulation of Ia afferent input during voluntary contraction in humans with spinal cord injury

    Bing Chen, Monica A Perez
    Physiological analysis reveals that during voluntary contraction Ia afferent input have a lesser facilitatory effect on motor neurons in humans with chronic incomplete spinal cord injury compared with control subjects.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Fuzzy supertertiary interactions within PSD-95 enable ligand binding

    George L Hamilton, Nabanita Saikia ... Mark E Bowen
    A combination of single molecule fluorescence with DMD simulations and disulfide mapping resolved the multistate structural landscape of the PSG supramodule from PSD-95, which explains how interdomain interactions within PSD-95 enable PDZ3 binding of the critical synaptic adhesion protein neuroligin.
    1. Microbiology and Infectious Disease

    LINE-1 retrotransposons facilitate horizontal gene transfer into poxviruses

    M Julhasur Rahman, Sherry L Haller ... Stefan Rothenburg
    A new experimental system of horizontal gene transfer demonstrates that host retrotransposons facilitate the transfer of host genes into poxviruses.
    1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    Poxviruses capture host genes by LINE-1 retrotransposition

    Sarah M Fixsen, Kelsey R Cone ... Nels C Elde
    Active selfish genetic elements in infected cells aid virus adaptation by catalyzing the transfer of host genes to virus genomes.
    1. Genetics and Genomics
    2. Stem Cells and Regenerative Medicine

    Highly efficient generation of isogenic pluripotent stem cell models using prime editing

    Hanqin Li, Oriol Busquets ... Frank Soldner
    A highly efficient platform to engineer designer mutations in human stem cells using RNA-based delivery of prime editing components.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Structural model of microtubule dynamics inhibition by kinesin-4 from the crystal structure of KLP-12 –tubulin complex

    Shinya Taguchi, Juri Nakano ... Ryo Nitta
    Structural, functional, and genetic analyses reveal how kinesin-4 motor KLP-12 precisely modulates the curvature of the microtubule plus-end to inhibit the microtubule dynamics for achieving the proper length control of axons.
    1. Biochemistry and Chemical Biology
    2. Physics of Living Systems

    Recovering mixtures of fast-diffusing states from short single-particle trajectories

    Alec Heckert, Liza Dahal ... Xavier Darzacq
    Tracking proteins in live cells is challenging due to technical limitations and biological complexity, but approaches based in Bayesian nonparametrics stand a decent chance at recovering a target protein's dynamic profile from noisy data.
    1. Cell Biology
    2. Immunology and Inflammation

    A crosstalk between hepcidin and IRE/IRP pathways controls ferroportin expression and determines serum iron levels in mice

    Edouard Charlebois, Carine Fillebeen ... Kostas Pantopoulos
    Under systemic iron overload, translational derepression of ferroportin mRNA via the IRE/IRP system antagonizes hepcidin-mediated ferroportin degradation to coordinately control serum iron.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Proton-transporting heliorhodopsins from marine giant viruses

    Shoko Hososhima, Ritsu Mizutori ... Hideki Kandori
    A viral heliorhodopsin from Emiliania huxleyi virus 202 (V2HeR3) is a light-activated proton transporter, which has the potential to depolarize the host cells by light, possibly to overcome the host defense mechanisms or to prevent superinfection.
    1. Neuroscience

    Machine learning-assisted fluoroscopy of bladder function in awake mice

    Helene De Bruyn, Nikky Corthout ... Thomas Voets
    A new combined approach using X-ray fluoroscopy and machine learning provides detailed new insights into bladder function in awake, unrestrained mice and unveils important limitations of current approaches to study bladder function.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3

    Alonso Javier Pardal, Andrew James Bowman
    Histone H3 can translocate to the nucleus as a monomer through a pathway governed by importin-5 and transfers to the histone chaperone NASP, having implications in the folding of H3-H4 dimers and, therefore, the kinetics of genome packaging during replication.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Distinct architectural requirements for the parS centromeric sequence of the pSM19035 plasmid partition machinery

    Andrea Volante, Juan Carlos Alonso, Kiyoshi Mizuuchi
    Unique sequence feature requirements for the plasmid centromere pSM19035-parS for ParApSM-ATPase activation byribbon-helix-helix-ParBpSM, a non-CTPase centromere-binding protein, evince complex interaction gymnastics among the three reaction components necessary for plasmid partition, which is distinct from ParABS-systems involving helix-turn-helix-ParB-CTPases.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Multiple ciliary localization signals control INPP5E ciliary targeting

    Dario Cilleros-Rodriguez, Raquel Martin-Morales ... Francesc R Garcia-Gonzalo
    Four conserved ciliary localization signals in the Joubert syndrome-associated INPP5E phosphoinositide phosphatase control its ciliary accumulation.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structure of Geobacter OmcZ filaments suggests extracellular cytochrome polymers evolved independently multiple times

    Fengbin Wang, Chi Ho Chan ... Daniel R Bond
    A cryo-EM structure of the bacterial OmcZ cytochrome filament provides evidence for the possibility that cytochrome polymers had multiple origins in bacteria.
    1. Neuroscience

    Molecular characteristics and laminar distribution of prefrontal neurons projecting to the mesolimbic system

    Ákos Babiczky, Ferenc Matyas
    Molecular, laminar, and regional characterization in combination with classical and conditional tracing techniques reveal that medial prefrontal cortical neurons innervating the nucleus accumbens and the ventral tegmental area form distinct populations.
    1. Neuroscience

    Amygdala-cortical collaboration in reward learning and decision making

    Kate M Wassum
    A review of a neuronal circuit architecture that helps us to learn about rewarding events and then to use that information to support adaptive decision making.
    1. Neuroscience

    Deep learning-based feature extraction for prediction and interpretation of sharp-wave ripples in the rodent hippocampus

    Andrea Navas-Olive, Rodrigo Amaducci ... Liset M de la Prida
    A new method is described to identify sharp-wave ripples from the rodent hippocampus with deep learning techniques, which may help to identify and characterize previously undetected physiological events.
    1. Biochemistry and Chemical Biology
    2. Cancer Biology

    Crosstalk between AML and stromal cells triggers acetate secretion through the metabolic rewiring of stromal cells

    Nuria Vilaplana-Lopera, Vincent Cuminetti ... Paloma Garcia
    Interplay between AML and stromal cells can initiate a mechanism involving gap junctions where ROS is transferred from cancer cells to stromal cells which then produce acetate which can in return be absorbed/utilised by the cancer cells.
    1. Microbiology and Infectious Disease

    Hemocyte differentiation to the megacyte lineage enhances mosquito immunity against Plasmodium

    Ana Beatriz F Barletta, Banhisikha Saha ... Carolina Barillas-Mury
    Toll signaling modulates hemocyte differentiation into the megacyte lineage and their recruitment to the midgut greatly enhances mosquito immunity against Plasmodium.
    1. Cell Biology
    2. Neuroscience

    Keratinocyte PIEZO1 modulates cutaneous mechanosensation

    Alexander R Mikesell, Olena Isaeva ... Cheryl L Stucky
    Epidermal PIEZO1 is a key keratinocyte mechanotransducer which mediates normal behavioral and sensory neuron responses to cutaneous mechanical stimulation.
    1. Medicine
    2. Neuroscience

    Stage-dependent differential influence of metabolic and structural networks on memory across Alzheimer’s disease continuum

    Kok Pin Ng, Xing Qian ... Alzheimer's Disease Neuroimaging Initiative
    The early influence of the breakdown of both hippocampal structural network and angular gyrus-seeded default mode metabolism network on memory performance underscore the importance of early intervention in preclinical Alzheimer’s disease.
    1. Medicine

    Brain atlas for glycoprotein hormone receptors at single-transcript level

    Vitaly Ryu, Anisa Gumerova ... Mone Zaidi
    The most comprehensive neuroanatomical atlas on the expression of three glycoprotein hormone receptors, namely, TSHRs, LHCGRs, and FSHRs, was mapped using RNAscope, a technology that allows the detection of mRNA at single-transcript level.
    1. Microbiology and Infectious Disease

    Vaccination decreases the risk of influenza A virus reassortment but not genetic variation in pigs

    Chong Li, Marie R Culhane ... Montserrat Torremorell
    Vaccination has the potential to decrease swine influenza diversification by restricting influenza virus co-infections and reassortment events in pigs.
    1. Genetics and Genomics

    Data-driven, participatory characterization of farmer varieties discloses teff breeding potential under current and future climates

    Aemiro Bezabih Woldeyohannes, Sessen Daniel Iohannes ... Matteo Dell'Acqua
    A genomic, climatic, agronomic, and participatory characterization of teff agrobiodiversity shows opportunities and challenges of teff improvement in the face of climate change.
    1. Cell Biology

    Viscoelastic properties of suspended cells measured with shear flow deformation cytometry

    Richard Gerum, Elham Mirzahossein ... Ben Fabry
    Under fluid shear, living cells deform elliptically, align in flow direction, and rotate, from which their frequency-dependent viscoelastic properties can be inferred.
    1. Biochemistry and Chemical Biology
    2. Evolutionary Biology

    Comprehensive phylogenetic analysis of the ribonucleotide reductase family reveals an ancestral clade

    Audrey A Burnim, Matthew A Spence ... Nozomi Ando
    A large-scale phylogenetic inference of the ribonucleotide reductase family reveals a new distinct clade with implications on how nature adapted to environmental changes.
    1. Plant Biology

    Uncovering natural variation in root system architecture and growth dynamics using a robotics-assisted phenomics platform

    Therese LaRue, Heike Lindner ... José R Dinneny
    Advances in robotics-assisted plant handling and automated image analysis enables the characterization of root growth dynamics for Arabidopsis accessions at late stages of development and the association of these traits with genetic variation and climatic variables.
    1. Cell Biology
    2. Neuroscience

    Chronic neurotransmission increases the susceptibility of lateral-line hair cells to ototoxic insults

    Daria Lukasz, Alisha Beirl, Katie Kindt
    In vivo imaging reveals that the synaptic vesicle cycle is a significant contributor to oxidative stress in sensory hair cells, rendering them susceptible to insults.
    1. Cell Biology

    The skeletal muscle circadian clock regulates titin splicing through RBM20

    Lance A Riley, Xiping Zhang ... Karyn A Esser
    The muscle circadian clock regulates RNA-binding motif 20 (Rbm20) gene expression, providing a novel mechanism for titin splicing and isoform expression with implications for muscle structure and function.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Most cancers carry a substantial deleterious load due to Hill-Robertson interference

    Susanne Tilk, Svyatoslav Tkachenko ... Christopher D McFarland
    The absence of negative selection observed in most cancer genomes can be explained by the intrinsic genome-wide linkage in somatic evolution and creates a substantial proteotoxic load.
    1. Cell Biology

    Ait1 regulates TORC1 signaling and localization in budding yeast

    Ryan L Wallace, Eric Lu ... Andrew P Capaldi
    The budding yeast gained a novel regulator of TORC1 signaling, called Ait1, 150–200 million years ago, around the same time they lost functional Rheb and Tsc1/2.
    1. Biochemistry and Chemical Biology

    Specific binding of Hsp27 and phosphorylated Tau mitigates abnormal Tau aggregation-induced pathology

    Shengnan Zhang, Yi Zhu ... Dan Li
    Structural basis and molecular mechanism of how molecular chaperone Hsp27 specifically captures phosphorylated Tau and prevents it from abnormal aggregation.