March 2023

Cover articles

    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    A code for RNA localization

    Raeann Goering, Ankita Arora ... J Matthew Taliaferro
    1. Developmental Biology
    2. Neuroscience

    A dual role for latrophilin-2

    Daniel T Pederick, Nicole A Perry-Hauser ... Liqun Luo
    1. Cell Biology
    2. Neuroscience

    Mitochondria in hair cells

    Andrea McQuate, Sharmon Knecht, David W Raible
    1. Chromosomes and Gene Expression
    2. Developmental Biology

    lncRNAs and the heart

    Valeria Taliani, Giulia Buonaiuto ... Monica Ballarino

Highlights controls:

Research articles

    1. Computational and Systems Biology
    2. Genetics and Genomics

    The potential of integrating human and mouse discovery platforms to advance our understanding of cardiometabolic diseases

    Aaron W Jurrjens, Marcus M Seldin ... Anna C Calkin
    The benefits of integrating cross-species systems genetics platforms to advance knowledge in the underlying mechanisms that drive cardiometabolic diseases have been investigated.
    1. Developmental Biology

    Kinetics of blood cell differentiation during hematopoiesis revealed by quantitative long-term live imaging

    Kevin Yueh Lin Ho, Rosalyn Leigh Carr ... Guy Tanentzapf
    A long-term multi-organ co-culture system coupled with quantitative imaging to visualize blood cell cycle progression, analyze polarized blood cell mitosis, and track blood cell differentiation kinetics during Drosophila hematopoiesis in homeostatic condition and following infection.
    1. Immunology and Inflammation

    Human thymopoiesis produces polyspecific CD8+ α/β T cells responding to multiple viral antigens

    Valentin Quiniou, Pierre Barennes ... David Klatzmann
    The thymic selection of the human T-cell receptor repertoire releases polyspecific receptors with the ability to recognize and respond to peptides from unrelated viruses.
    1. Developmental Biology
    2. Microbiology and Infectious Disease

    Hypoxia-induced tracheal elasticity in vector beetle facilitates the loading of pinewood nematode

    Xuan Tang, Jiao Zhou ... Jianghua Sun
    An insect-transmissible plant pathogen, Bursaphelenchus xylophilus, uniquely excavates hypoxia to increase vector capacity via Muc91C-enhanced elasticity of tracheal tubes.
    1. Neuroscience

    Model-based whole-brain perturbational landscape of neurodegenerative diseases

    Yonatan Sanz Perl, Sol Fittipaldi ... Enzo Tagliazucchi
    The combination of deep learning with whole-brain computational models reveals the low-dimensional representation of neurodegenerative diseases, which emerges from a highly multidimensional brain, providing valuable insight into pathological states' diagnostic, prognosis, and treatment response.
    1. Cell Biology

    Autofluorescence imaging permits label-free cell type assignment and reveals the dynamic formation of airway secretory cell associated antigen passages (SAPs)

    Viral S Shah, Jue Hou ... Jayaraj Rajagopal
    Autofluorescence imaging permits label-free cell type identification in live airway tissue and reveals the dynamic formation of airway secretory cell associated antigen passages.
    1. Chromosomes and Gene Expression

    RNA Polymerase II transcription independent of TBP in murine embryonic stem cells

    James ZJ Kwan, Thomas F Nguyen ... Sheila S Teves
    TATA-box binding protein is not required for RNA Polymerase II transcription in mouse embryonic stem cells.
    1. Cell Biology

    Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation

    Danielle B Buglak, Pauline Bougaran ... Victoria L Bautch
    The nuclear membrane protein SUN1 stabilizes endothelial cell-cell junctions far from the nucleus via regulation of microtubule dynamics and Rho GEF-H1 signaling, revealing long-range cellular communication important for vascular development and function.
    1. Stem Cells and Regenerative Medicine
    2. Developmental Biology

    Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoids

    Jaydeep Sidhaye, Philipp Trepte ... Jürgen A Knoblich
    RNA-protein multiome approach helps to discover that the posttranscriptional regulation of the translational machinery is crucial for the fidelity of cortical development.
    1. Genetics and Genomics
    2. Medicine

    Phenome-wide Mendelian randomization study of plasma triglyceride levels and 2600 disease traits

    Joshua K Park, Shantanu Bafna ... Ron Do
    Phenome-wide estimation of causality between plasma triglyceride levels and disease risk highlights associations with atherosclerotic disease and other unrelated diseases.
    1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    Competition between lysogenic and sensitive bacteria is determined by the fitness costs of the different emerging phage-resistance strategies

    Olaya Rendueles, Jorge AM de Sousa, Eduardo PC Rocha
    The emergence and evolution of different phage-resistance strategies during coevolution between a phage-sensitive strain and a polylysogenic competitor depend on the amount of phage pressure, the fitness costs of resistance, and how these may change at different time scales.
    1. Computational and Systems Biology

    Longitudinal fundus imaging and its genome-wide association analysis provide evidence for a human retinal aging clock

    Sara Ahadi, Kenneth A Wilson ... Ali Bashir
    Deep learning on fundus images provides a non-invasive, short time-scale biological aging clock that successfully identifies aging genes validated in a fly model.
    1. Structural Biology and Molecular Biophysics

    Cryo-EM reveals an unprecedented binding site for NaV1.7 inhibitors enabling rational design of potent hybrid inhibitors

    Marc Kschonsak, Christine C Jao ... John C Tellis
    Cryogenic electron microscopy enables real-time structure-based drug design to advance the discovery of novel classes of small molecule inhibitors.
    1. Neuroscience

    Generative network modeling reveals quantitative definitions of bilateral symmetry exhibited by a whole insect brain connectome

    Benjamin D Pedigo, Mike Powell ... Joshua T Vogelstein
    Novel statistical tests to compare networks enable the evaluation of mathematical definitions of left/right symmetry in a larval Drosophila brain connectome.
    1. Biochemistry and Chemical Biology

    Reserpine maintains photoreceptor survival in retinal ciliopathy by resolving proteostasis imbalance and ciliogenesis defects

    Holly Y Chen, Manju Swaroop ... Anand Swaroop
    High-throughput screening of over 6000 drugs using cells and retina tissue with a CEP290 ciliopathy mutation identified a small molecule, reserpine, which enhanced photoreceptor survival in retinal organoids and in a mouse disease model by partially restoring balance in proteostasis.
    1. Medicine

    Study of efficacy and longevity of immune response to third and fourth doses of COVID-19 vaccines in patients with cancer: A single arm clinical trial

    Astha Thakkar, Kith Pradhan ... Balazs Halmos
    Third dose of COVID-19 vaccine leads to seroconversion in 56% cancer patients that are seronegative after primary vaccination and a fourth can further boost immune response in patients with hematologic malignancies, which can be predicted by IgM and CD19 levels.
    1. Neuroscience

    Oligodendrocyte-mediated myelin plasticity and its role in neural synchronization

    Sinisa Pajevic, Dietmar Plenz ... R Douglas Fields
    A biologically plausible model of myelin plasticity demonstrates how oligodendrocytes can selectively adjust conduction delays in axon bundles to allow robust communication between brain regions through long-range synchronization.
    1. Developmental Biology
    2. Neuroscience

    Origin of wiring specificity in an olfactory map revealed by neuron type–specific, time-lapse imaging of dendrite targeting

    Kenneth Kin Lam Wong, Tongchao Li ... Liqun Luo
    Advanced genetics and imaging reveal wiring logic underlying the olfactory map organization in the developing fruit fly brain, and strategies employed by projection neurons to target dendrites to specific locations in a timely manner.
    1. Developmental Biology

    Histone deacetylase 1 maintains lineage integrity through histone acetylome refinement during early embryogenesis

    Jeff Jiajing Zhou, Jin Sun Cho ... Ken WY Cho
    Histone deacetylase 1 (Hdac1) plays a crucial role in maintaining differential histone acetylation states of enhancers in various germ layers and regulates the transcriptional program both temporally and spatially.
    1. Developmental Biology
    2. Genetics and Genomics

    Evidence for RNA or protein transport from somatic tissues to the male reproductive tract in mouse

    Vera Rinaldi, Kathleen Messemer ... Oliver J Rando
    Tissue-specific Cre recombinase often drives 'off-target' reporter activity in the male reproductive tract, suggesting the potential for inter-tissue RNA or protein trafficking in control of sperm maturation or other aspects of reproductive physiology.
    1. Biochemistry and Chemical Biology

    Targeting oncogenic KRasG13C with nucleotide-based covalent inhibitors

    Lisa Goebel, Tonia Kirschner ... Daniel Rauh
    The important oncogene KRasG13C can be targeted by covalently binding nucleotide-analogues, resulting in a locked protein that can no longer induce oncogenic signaling.
    1. Neuroscience

    Schema-based predictive eye movements support sequential memory encoding

    Jiawen Huang, Isabel Velarde ... Christopher Baldassano
    The development of knowledge for a board game facilitates memory for sequences in the game, demonstrating a new mechanism for the facilitation by enabling complicated predictions.
    1. Neuroscience

    Extra-hippocampal contributions to pattern separation

    Tarek Amer, Lila Davachi
    The ability to discriminate highly overlapping events in memory is a multistage process supported by a network of brain regions and neocortical–hippocampal interactions.
    1. Medicine
    2. Neuroscience

    The role of expectations, control and reward in the development of pain persistence based on a unified model

    Christian Büchel
    The integration of established psychological and biological models of pain suggests novel interventions to prevent pain persistence.
    1. Immunology and Inflammation

    Characterisation of the immune repertoire of a humanised transgenic mouse through immunophenotyping and high-throughput sequencing

    Eve Richardson, Špela Binter ... Simon J Watson
    A commonly used immunoglobulin loci-transgenic mouse, the Kymouse, has B cells that go through complete development and have receptor repertoires that show key differences in encoding gene frequencies but structural similarity in their most variable regions.
    1. Immunology and Inflammation

    Antigenic mapping and functional characterization of human New World hantavirus neutralizing antibodies

    Taylor B Engdahl, Elad Binshtein ... James E Crowe
    Human antibodies that neutralize New World hantaviruses are promising for prevention or treatment of these high-priority emerging pathogens carried by rodents and transmitted to humans by aerosolized excreta or, in rare cases, person-to-person contact.
    1. Epidemiology and Global Health
    2. Genetics and Genomics

    Polygenic risk scores for the prediction of common cancers in East Asians: A population-based prospective cohort study

    Peh Joo Ho, Iain BeeHuat Tan ... Jingmei Li
    Site-specific polygenic risk scores can stratify the risk of developing breast, prostate, and colorectal cancers in this East Asian population.
    1. Cell Biology
    2. Developmental Biology

    Transcriptional drifts associated with environmental changes in endothelial cells

    Yalda Afshar, Feyiang Ma ... M Luisa Iruela-Arispe
    Nearly half of the transcriptome is altered when endothelial cells transition in vitro, expression patterns for some genes are regained by exposing cells to shear stress and others through exposure to smooth muscle cells.
    1. Neuroscience

    Neuroelectrophysiology-Compatible Electrolytic Lesioning

    Iliana E. Bray, Stephen E. Clarke ... the Brain Interfacing Laboratory
    1. Immunology and Inflammation

    PTPN22 R620W gene editing in T cells enhances low-avidity TCR responses

    Warren Anderson, Fariba Barahmand-pour-Whitman ... David J Rawlings
    Gene editing to generate precisely matched primary human T cell populations demonstrates that the common autoimmune risk allele in the tyrosine phosphatase, PTPN22, promotes signaling in cells expressing a low-avidity, self-reactive TCR specific for a diabetes-associated self-antigen.
    1. Neuroscience

    Memory for incidentally learned categories evolves in the post-learning interval

    Yafit Gabay, Avi Karni, Lori L Holt
    Incidental experiences can lead to lasting category knowledge, demonstrating that humans forage for information to acquire and consolidate new knowledge even when learning is not strictly necessary for success on an ongoing task.
    1. Neuroscience

    Spatiotemporal organisation of human sensorimotor beta burst activity

    Catharina Zich, Andrew J Quinn ... Sven Bestmann
    Burst activity can be described in all three signal domains and sensorimotor beta burst activity propagates along two axes either parallel or perpendicular to the central sulcus.
    1. Biochemistry and Chemical Biology
    2. Cancer Biology

    Multi-targeted therapy resistance via drug-induced secretome fucosylation

    Mark Borris D Aldonza, Junghwa Cha ... Yoosik Kim
    Targeted therapies induce an aberrant fucosylation of complex tumor secretomes stimulating the expansion of minority drug-resistant clones and promoting therapy resistance.
    1. Neuroscience

    Cortical magnification eliminates differences in contrast sensitivity across but not around the visual field

    Michael Jigo, Daniel Tavdy ... Marisa Carrasco
    Scaling stimulus size based on the extent of its representation in visual cortex eliminates differences in contrast sensitivity and acuity when measured as a function of eccentricity, but not when measured as a function of polar angle.
    1. Cell Biology

    Defects in lipid homeostasis reflect the function of TANGO2 in phospholipid and neutral lipid metabolism

    Agustin Leonardo Lujan, Ombretta Foresti ... Vivek Malhotra
    TANGO2 emerges as a key regulator of fatty acid metabolism by likely affecting acyl-CoA production and utilization.
    1. Cell Biology
    2. Immunology and Inflammation

    HUWE1 controls tristetraprolin proteasomal degradation by regulating its phosphorylation

    Sara Scinicariello, Adrian Soderholm ... Gijs A Versteeg
    HUWE1 indirectly mediates the return to cellular homeostasis after pro-inflammatory responses, which it does by controling the stability of the immune suppressor tristetraprolin.
    1. Cancer Biology
    2. Microbiology and Infectious Disease

    Evidence for virus-mediated oncogenesis in bladder cancers arising in solid organ transplant recipients

    Gabriel J Starrett, Kelly Yu ... Eric A Engels
    Nearly half of bladder cancers of solid organ transplant recipients harbor papillomaviruses or polyomaviruses, with many tumors showing evidence of clonal viral integration and viral oncogene effects on tumor gene expression patterns.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Enterobacterales plasmid sharing amongst human bloodstream infections, livestock, wastewater, and waterway niches in Oxfordshire, UK

    William Matlock, Samuel Lipworth ... REHAB Consortium
    A geographically and temporally restricted genomic surveillance study concludes that Enterobacterales plasmid dissemination between human and non-human niches might be occurring at greater rates than previously estimated.
    1. Genetics and Genomics
    2. Neuroscience

    Nutrigenomic regulation of sensory plasticity

    Hayeon Sung, Anoumid Vaziri ... Monica Dus
    Nutrient and activity-responsive pathways converge on chromatin to drive neural adaptations to the food environment.
    1. Neuroscience

    Open-source tools for behavioral video analysis: Setup, methods, and best practices

    Kevin Luxem, Jennifer J Sun ... Mark Laubach
    Methods for video analysis of behaving animals are described, with guidance provided on setting up the methods in a research laboratory and best practices for experimenters and developers.
    1. Structural Biology and Molecular Biophysics

    Resting mitochondrial complex I from Drosophila melanogaster adopts a helix-locked state

    Abhilash Padavannil, Anjaneyulu Murari ... James A Letts
    Molecular structure of mitochondrial complex I from Drosophila melanogaster reveals a structural element not seen in other organisms to date that may regulate the transition between an off-pathway resting state and the active state of the complex.
    1. Cancer Biology
    2. Medicine

    A survey of open questions in adaptive therapy: Bridging mathematics and clinical translation

    Jeffrey West, Fred Adler ... Alexander RA Anderson
    Open questions of cancer adaptive therapy are discussed through the lens of mathematical modeling, categorized into three sections, (1) integrating appropriate components into mathematical models, (2) dosing protocol design and validation, and (3) challenges and opportunities in clinical translation.
    1. Cell Biology
    2. Neuroscience

    UBQLN2 restrains the domesticated retrotransposon PEG10 to maintain neuronal health in ALS

    Holly H Black, Jessica L Hanson ... Alexandra M Whiteley
    Exploring how UBQLN2 mutations cause ALS leads to the discovery of a pathway by which an ancient retroelement changes gene expression in human cells.
    1. Cell Biology

    Lipid hydroperoxides promote sarcopenia through carbonyl stress

    Hiroaki Eshima, Justin L Shahtout ... Katsuhiko Funai
    Genetic or pharmacological suppression of lipid peroxidation pathway protects skeletal muscle from disuse-induced atrophy and weakness in young and old mice.
    1. Plant Biology
    2. Computational and Systems Biology

    The photosystem I supercomplex from a primordial green alga Ostreococcus tauri harbors three light-harvesting complex trimers

    Asako Ishii, Jianyu Shan ... Jun Minagawa
    The structure of the photosystem I (PSI) supercomplex in Ostreococcus tauri has revealed a unique hybrid of both plant-type and green algal-type PSI supercomplexes with three Lhcp trimers located at the ‘state 2’ position.
    1. Chromosomes and Gene Expression

    X-chromosome target specificity diverged between dosage compensation mechanisms of two closely related Caenorhabditis species

    Qiming Yang, Te-Wen Lo ... Barbara J Meyer
    Regulatory hierarchies controlling sex determination and X-chromosome dosage compensation between closely-related nematode species are conserved, but X-chromosome target specificity for the condensin dosage compensation complex has diverged, thereby contributing to reproductive isolation.
    1. Genetics and Genomics
    2. Evolutionary Biology

    Regulatory and coding sequences of TRNP1 co-evolve with brain size and cortical folding in mammals

    Zane Kliesmete, Lucas Esteban Wange ... Wolfgang Enard
    Combining phylogenetic and functional approaches, it is shown that the protein sequence, the proliferative activity, and a regulatory element of TRNP1 co-evolve with brain size and folding.
    1. Microbiology and Infectious Disease

    Promoting Fc-Fc interactions between anti-capsular antibodies provides strong immune protection against Streptococcus pneumoniae

    Leire Aguinagalde Salazar, Maurits A den Boer ... Suzan HM Rooijakkers
    Fc engineering of monoclonal antibodies strongly improves immune clearance of pneumococcus.
    1. Biochemistry and Chemical Biology
    2. Neuroscience

    Glia-neuron coupling via a bipartite sialylation pathway promotes neural transmission and stress tolerance in Drosophila

    Hilary Scott, Boris Novikov ... Vladislav Panin
    The sialylation pathway is uniquely partitioned in Drosophila between glia and neurons and mediates a novel mechanism of glia-neuron coupling that regulates neural functions, promotes tolerance to heat and oxidative stress, and maintains the normal level of voltage-gated sodium channels.
    1. Neuroscience

    On the normative advantages of dopamine and striatal opponency for learning and choice

    Alana Jaskir, Michael J Frank
    A computational model of the opponent neural architecture the basal ganglia, in tandem with adaptive dopamine modulation, exhibits robust advantages over traditional learning algorithms and ties together seemingly aberrant behavioral patterns resulting from dopamine and environmental manipulations across species.
    1. Epidemiology and Global Health
    2. Immunology and Inflammation

    Pathogenic mechanisms of post-acute sequelae of SARS-CoV-2 infection (PASC)

    Zaki A Sherif, Christian R Gomez ... RECOVER Mechanistic Pathway Task Force
    PASC (post-acute sequelae of SARS-CoV-2 infection) is an enduring and debilitating illness caused by a persistent virus that promotes inflammation, coagulation, and autoimmunity complications in millions of patients initially diagnosed with COVID-19, but with no known standardized treatment regimen.
    1. Developmental Biology

    The Slingshot phosphatase 2 is required for acrosome biogenesis during spermatogenesis in mice

    Ke Xu, Xianwei Su ... Hongbin Liu
    SSH2, a Slingshot phosphatase required for male fertility, participates in acrosome biogenesis via the control of actin remodeling during spermatid development.
    1. Epidemiology and Global Health

    Impact of COVID-19 pandemic on breast and cervical cancer screening in Denmark: A register-based study

    Mette Hartmann Nonboe, George Napolitano ... Elsebeth Lynge
    Denmark continued cancer screening during the pandemic, but following the first lockdown a temporary drop was seen in breast and cervical screening activity.
    1. Structural Biology and Molecular Biophysics

    Structures of ferroportin in complex with its specific inhibitor vamifeport

    Elena Farah Lehmann, Márton Liziczai ... Cristina Manatschal
    The structure of the human iron exporter ferroportin in complex with its inhibitor vamifeport, which is currently in clinical development against β-thalassemia and sickle cell disease, provides insight into pharmacological mechanisms.
    1. Neuroscience

    Recurrent network interactions explain tectal response variability and experience-dependent behavior

    Asaph Zylbertal, Isaac H Bianco
    Recurrent interactions in the optic tectum underlie activity-dependent changes in network state that account for variability in sensory encoding and behavior.
    1. Developmental Biology
    2. Evolutionary Biology

    A novel gene REPTOR2 activates the autophagic degradation of wing disc in pea aphid

    Erliang Yuan, Huijuan Guo ... Yucheng Sun
    The novel function of the evolutionarily conserved nutrient-sensing pathway TOR in the wing disc-specific autophagic degradation and development of wingless aphids.
    1. Cancer Biology

    Osteosarcoma-enriched transcripts paradoxically generate osteosarcoma-suppressing extracellular proteins

    Kexin Li, Qingji Huo ... Hiroki Yokota
    The anti-tumor action of engineered mesenchymal stem cells highlights the double-edged role of oncoproteins in osteosarcoma, and suggests the possibility of developing a novel strategy for protein-based cancer therapy.
    1. Biochemistry and Chemical Biology

    Identification of a conserved S2 epitope present on spike proteins from all highly pathogenic coronaviruses

    Rui P Silva, Yimin Huang ... Jennifer A Maynard
    A new class of antibody binding a highly conserved epitope on the spike S2 domain of MERS-CoV, SARS-CoV, and SARS-CoV-2 viruses is described.
    1. Cell Biology
    2. Cancer Biology

    Purinergic GPCR-integrin interactions drive pancreatic cancer cell invasion

    Elena Tomas Bort, Megan D Joseph ... Richard P Grose
    New role of P2Y2-αV integrin interactions in ATP-driven pancreatic cancer cell invasion provides a potential anti-metastatic target for pancreatic cancer.
    1. Cell Biology

    Actin-regulated Siglec-1 nanoclustering influences HIV-1 capture and virus-containing compartment formation in dendritic cells

    Enric Gutiérrez-Martínez, Susana Benet Garrabé ... Maria F Garcia-Parajo
    Advanced imaging methods reveal that Siglec-1 spatial distribution on mature dendritic cells is regulated by components of the actin polymerization machinery impacting on its engagement to HIV-particles and virus sequestration toward virus-containing compartments.
    1. Evolutionary Biology
    2. Genetics and Genomics

    The lingering effects of Neanderthal introgression on human complex traits

    Xinzhu Wei, Christopher R Robles ... Sriram Sankararaman
    Genetic variants introgressed into modern humans from Neanderthals tend to be depleted in their contribution to heritable trait variation relative to modern human variants consistent with the action of purifying selection.
    1. Genetics and Genomics

    Native functions of short tandem repeats

    Shannon E Wright, Peter K Todd
    The mechanisms by which non-pathologic short tandem repeats normally influence gene expression and engender rapid genetic variation across phylogeny directly inform how expansions in these same repeats elicit neurological disease.
    1. Developmental Biology
    2. Evolutionary Biology

    Ectodermal Wnt signaling, cell fate determination, and polarity of the skate gill arch skeleton

    Jenaid M Rees, Victoria A Sleight ... J Andrew Gillis
    Spatially distinct ectodermal Wnt and endodermal Shh signals serve to polarize the skate gill skeleton.
    1. Structural Biology and Molecular Biophysics

    Ion permeation pathway within the internal pore of P2X receptor channels

    Stephanie W Tam, Kate Huffer ... Kenton J Swartz
    Mapping the ion permeation pathway within the internal pore of ATP-activated P2X receptor channels.
    1. Developmental Biology
    2. Neuroscience

    Context-dependent requirement of G protein coupling for Latrophilin-2 in target selection of hippocampal axons

    Daniel T Pederick, Nicole A Perry-Hauser ... Liqun Luo
    Latrophilin-2 G protein coupling is required in axons but not target neurons for neural circuit assembly in the mouse hippocampus.
    1. Computational and Systems Biology
    2. Neuroscience

    Multilayer brain networks can identify the epileptogenic zone and seizure dynamics

    Hossein Shahabi, Dileep R Nair, Richard M Leahy
    Aging and the duration of epilepsy can intensify the high-frequency desynchronization between the epileptogenic zone and the rest of the brain in early to mid-seizure.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Timeline of changes in spike conformational dynamics in emergent SARS-CoV-2 variants reveal progressive stabilization of trimer stalk with altered NTD dynamics

    Sean M Braet, Theresa SC Buckley ... Ganesh S Anand
    Structural mass spectrometry reveals conformational changes in emerging SARS-CoV-2 spike protein variants that correlate with increased viral fitness.
    1. Neuroscience

    Netrin-1 regulates the balance of synaptic glutamate signaling in the adult ventral tegmental area

    Marcella M Cline, Barbara Juarez ... Larry S Zweifel
    Genetic analysis of Ntn1 in adult mouse midbrain neurons reveals its function in maintaining excitatory synapses, loss of Ntn1 function in inhibitory neurons is significantly detrimental to mesolimbic system function.
    1. Neuroscience

    A parabrachial to hypothalamic pathway mediates defensive behavior

    Fan Wang, Yuge Chen ... Zhihua Gao
    Neural circuit research reveals a previously unrecognized role for the LPBCCK-PVN pathway in controlling defensive behaviors.
    1. Computational and Systems Biology
    2. Neuroscience

    Asymmetric retinal direction tuning predicts optokinetic eye movements across stimulus conditions

    Scott C Harris, Felice A Dunn
    Retinal physiology and anatomy and visual behavior reveal how sensory circuits in the retina can shape an organism’s eye movements over a range of ethologically relevant stimulus conditions.
    1. Immunology and Inflammation

    Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata

    Rimma Laufer Britva, Aviad Keren ... Amos Gilhar
    Innate lymphoid cells type 1 suffice to induce hallmarks of alopecia areata phenotype in organ-cultured human scalp hair follicles ex vivo and in human scalp skin xenotransplants in vivo, suggesting that these cells play a role in early AA pathogenesis.
    1. Physics of Living Systems

    Lonafarnib improves cardiovascular function and survival in a mouse model of Hutchinson-Gilford progeria syndrome

    Sae-Il Murtada, Nicole Mikush ... Jay D Humphrey
    Daily administration of the farnesyltransferase inhibitor lonafarnib decreases central artery stiffness, increases distal artery vasoactivity, and improves left ventricular function consistent with increased survival in a mouse model of Hutchinson-Gilford progeria syndrome.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Concurrent remodelling of nucleolar 60S subunit precursors by the Rea1 ATPase and Spb4 RNA helicase

    Valentin Mitterer, Matthias Thoms ... Roland Beckmann
    The RNA helicase Spb4 restructures rRNA helices H62/H63 and primes pre-ribosomal 60S intermediates for large-scale remodelling by the AAA-ATPase Rea1.
    1. Biochemistry and Chemical Biology

    High-throughput profiling of sequence recognition by tyrosine kinases and SH2 domains using bacterial peptide display

    Allyson Li, Rashmi Voleti ... Neel H Shah
    A high-throughput method to profile tyrosine kinases and phosphotyrosine recognition domains reveals new rules for sequence specificity and maps the effects of mutations on the recognition of tyrosine phosphorylation sites.
    1. Epidemiology and Global Health
    2. Immunology and Inflammation

    Evaluation of antibody kinetics and durability in healthy individuals vaccinated with inactivated COVID-19 vaccine (CoronaVac): A cross-sectional and cohort study in Zhejiang, China

    Hangjie Zhang, Qianhui Hua ... Huakun Lv
    Serum antibodies induced by CoronaVac tended to decrease over time, even though vaccinated with the homologous booster dose, and Delta and Omicron variants may be able to more efficiently evade the antibodies with time.
    1. Neuroscience

    Neural learning rules for generating flexible predictions and computing the successor representation

    Ching Fang, Dmitriy Aronov ... Emily L Mackevicius
    A recurrent network using a simple, biologically plausible learning rule can learn the successor representation, suggesting that long-horizon predictions are computations that are easily accessible in neural circuits.
    1. Computational and Systems Biology

    Learning predictive cognitive maps with spiking neurons during behavior and replays

    Jacopo Bono, Sara Zannone ... Claudia Clopath
    Predictive cognitive maps can be learned during behavior and replays using in spiking neurons.
    1. Neuroscience

    Rapid learning of predictive maps with STDP and theta phase precession

    Tom M George, William de Cothi ... Caswell Barry
    A close approximation to the successor representation is learnt by a simple spike-time-dependent learning rule between cells undergoing theta phase precession.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities

    Giuseppina Mastrototaro, Pierluigi Carullo ... Marie-Louise Bang
    FHOD1 and CARP/Ankrd1 interact with the N-terminal region of palladin and cardiomyocyte-specific deletion of palladin in adult, but not embryonic, mice leads to dilated cardiomyopathy associated with intercalated disc widening.
    1. Evolutionary Biology
    2. Microbiology and Infectious Disease

    Rescue of Escherichia coli auxotrophy by de novo small proteins

    Arianne M Babina, Serhiy Surkov ... Michael Knopp
    De novo-generated small proteins can cause deattenuation of an amino acid biosynthetic operon by direct protein–RNA interactions.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Lineage-specific differences and regulatory networks governing human chondrocyte development

    Daniel Richard, Steven Pregizer ... April M Craft
    The transcriptomic and epigenetic landscapes of developing human articular and growth plate cartilage reveal putative gene regulatory networks governing lineage commitment and tissue homeostasis.
    1. Cell Biology

    Diet-induced loss of adipose hexokinase 2 correlates with hyperglycemia

    Mitsugu Shimobayashi, Amandine Thomas ... Michael N Hall
    A proteomics screening in mouse adipose tissue shows that loss of adipose Hexokinase 2 is a mechanism of obesity-induced insulin insensitivity.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Selection of HIV-1 for resistance to fifth-generation protease inhibitors reveals two independent pathways to high-level resistance

    Ean Spielvogel, Sook-Kyung Lee ... Ronald Swanstrom
    New HIV-1 protease inhibitor designs result in more potent inhibitors with high genetic barriers to resistance and the ability to lead virus evolution down less fit pathways when resistance occurs.
    1. Cell Biology
    2. Developmental Biology

    Inactivation of Invs/Nphp2 in renal epithelial cells drives infantile nephronophthisis like phenotypes in mouse

    Yuanyuan Li, Wenyan Xu ... Zhaoxia Sun
    Genetic and chemical genetic analysis pinpoint defective epithelial cells as the driver for NPHP like phenotypes in Invs mutants, demonstrate genetic interaction between Invs and Ift88 and identify the HDAC inhibitor valproic acid as a suppressor for Invs mutant phenotypes.
    1. Medicine

    An herbal drug combination identified by knowledge graph alleviates the clinical symptoms of plasma cell mastitis patients: A nonrandomized controlled trial

    Caigang Liu, Hong Yu ... Yongliang Yang
    Knowledge graph identified an herbal drug combination for the treatment of plasma cell mastitis.
    1. Genetics and Genomics

    Reassessment of weak parent-of-origin expression bias shows it rarely exists outside of known imprinted regions

    Carol A Edwards, William MD Watkinson ... Anne C Ferguson-Smith
    Genes with parent-of-origin specific expression are not as numerous as previously reported and validated parentally-biased expressed genes tend to be peripheral to known imprinted domains indicating shared regional control.
    1. Neuroscience

    A model of hippocampal replay driven by experience and environmental structure facilitates spatial learning

    Nicolas Diekmann, Sen Cheng
    A model of hippocampal replay is proposed that gives a biologically plausible account of how the hippocampus could prioritize replay and produce a variety of different replay statistics, and is efficient in driving spatial learning.
    1. Immunology and Inflammation

    Fever integrates antimicrobial defences, inflammation control, and tissue repair in a cold-blooded vertebrate

    Farah Haddad, Amro M Soliman ... Daniel R Barreda
    Behavioural, molecular, and functional analyses revealed that fever contributes to induction and resolution phases of acute inflammation, through enhancement of antimicrobial defences, increased efficiency in inflammation control, and improved wound repair.
    1. Cell Biology

    Novel repertoire of tau biosensors to monitor pathological tau transformation and seeding activity in living cells

    Erika Cecon, Atsuro Oishi ... Ralf Jockers
    Development of nanoluciferase complementation-based tau biosensors to detect tau conformational change and oligomerization in living cells in high-throughput assays.
    1. Neuroscience

    Neural circuits underlying habituation of visually evoked escape behaviors in larval zebrafish

    Haleh Fotowat, Florian Engert
    A novel neural mechanism is proposed and validated in larval zebrafish to explain how animals habituate to repeated looming stimulation.
    1. Immunology and Inflammation

    A lncRNA identifies Irf8 enhancer element in negative feedback control of dendritic cell differentiation

    Huaming Xu, Zhijian Li ... Martin Zenke
    A novel lncRNA marks the Irf8 downstream enhancer that is crucial for differentiation of plasmacytoid dendritic cells (pDC) and classical dendritic cells type 1 (cDC1) and confers feedback inhibition of Irf8 transcription.
    1. Microbiology and Infectious Disease

    Plasmodium falciparum adapts its investment into replication versus transmission according to the host environment

    Abdirahman I Abdi, Fiona Achcar ... Matthias Marti
    We investigate how parasites adapt to environmental changes in the context of changing transmission patterns, for example by increasing investment in transmission stages at low endemicity such as elimination settings.
    1. Neuroscience

    The cellular architecture of memory modules in Drosophila supports stochastic input integration

    Omar A Hafez, Benjamin Escribano ... Jan Pielage
    Computational modeling of a central decision neuron of Drosophila reveals an electrotonically compact architecture that is ideally suited to support efficient memory storage within a stochastically connected memory circuit.
    1. Neuroscience
    2. Physics of Living Systems

    Beta oscillations and waves in motor cortex can be accounted for by the interplay of spatially structured connectivity and fluctuating inputs

    Ling Kang, Jonas Ranft, Vincent Hakim
    The observed activity of the motor cortex in space and time is reproduced by a model with a specific structure of the inputs to the motor cortex.
    1. Neuroscience

    Emergence of time persistence in a data-driven neural network model

    Sebastien Wolf, Guillaume Le Goc ... Rémi Monasson
    A data-driven model of the neural activity of a circuit essential to swimming orientation in zebrafish can be mathematically interpreted to unveil the dynamical mechanism leading to persistence in tail orientation.
    1. Physics of Living Systems

    Vein fate determined by flow-based but time-delayed integration of network architecture

    Sophie Marbach, Noah Ziethen ... Karen Alim
    Relative hydraulic resistance, shear rate, and pressure in a vascular network integrate the network's architecture via fluid flow, and determine vein dynamics, with a time delay, in the prototypical organism Physarum polycephalum.
    1. Structural Biology and Molecular Biophysics

    Structural basis for the Rad6 activation by the Bre1 N-terminal domain

    Meng Shi, Jiaqi Zhao ... Song Xiang
    The structure of Bre1’s Rad6-binding domain in complex with Rad6 provides insights into the Bre1 and Rad6-catalyzed histone protein H2B mono-ubiquitination.
    1. Neuroscience

    Evidence for embracing normative modeling

    Saige Rutherford, Pieter Barkema ... Andre F Marquand
    A brain chart database, containing functional and structural normative models, was tested to compare normative modeling features to raw features across three benchmarking tasks including group difference testing, classification, and regression.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Mechanosensitive pore opening of a prokaryotic voltage-gated sodium channel

    Peter R Strege, Luke M Cowan ... Arthur Beyder
    Force applied to the cell membrane reversibly changes a voltage-insensitive gating step of a prokaryotic voltage-gated sodium channel.
    1. Cell Biology

    Meisosomes, folded membrane microdomains between the apical extracellular matrix and epidermis

    Dina Aggad, Nicolas Brouilly ... Nathalie Pujol
    Combining live imaging and advanced electron microscopy gives insights into the structural integrity of the nematode epidermis and its intimate connection with the extracellular matrix.
    1. Physics of Living Systems

    Emergent periodicity in the collective synchronous flashing of fireflies

    Raphael Sarfati, Kunaal Joshi ... Orit Peleg
    1. Immunology and Inflammation

    Cell circuits between leukemic cells and mesenchymal stem cells block lymphopoiesis by activating lymphotoxin beta receptor signaling

    Xing Feng, Ruifeng Sun ... Joao Pedro Pereira
    Acute lymphoblastic and myeloblastic leukemias activate lymphotoxin beta receptor in mesenchymal stem cells in the bone marrow to turn off interleukin-7 production and lymphopoiesis and gain competitive advantage.
    1. Neuroscience

    Landmark-based spatial navigation across the human lifespan

    Marcia Bécu, Denis Sheynikhovich ... Angelo Arleo
    Landmark cues preclude complex and flexible spatial navigation in human development and aging.
    1. Cell Biology
    2. Neuroscience

    Activity regulates a cell type-specific mitochondrial phenotype in zebrafish lateral line hair cells

    Andrea McQuate, Sharmon Knecht, David W Raible
    The highly metabolically active hair cells of the zebrafish lateral line have a distinct mitochondrial phenotype consisting of small mitochondria apically and large, networked mitochondrion basally, demonstrating a nonuniform mitochondrial architecture that is sculpted by cellular activity.
    1. Evolutionary Biology

    Dating the origin and spread of specialization on human hosts in Aedes aegypti mosquitoes

    Noah H Rose, Athanase Badolo ... Carolyn S McBride
    The dengue and yellow fever mosquito first specialized on humans about 5000 years ago, but appears to use the same genes to thrive in urban environments today.
    1. Neuroscience

    Genetic dissection of mutual interference between two consecutive learning tasks in Drosophila

    Jianjian Zhao, Xuchen Zhang ... Qian Li
    Proactive interference is regulated by the CSW/Raf/MAPK pathway, while retroactive interference is regulated by the Rac1 pathway.
    1. Microbiology and Infectious Disease
    2. Physics of Living Systems

    An optimal regulation of fluxes dictates microbial growth in and out of steady state

    Griffin Chure, Jonas Cremer
    Microbial cells optimally structure their proteomes in order to mutually maximize metabolism and translation, as established by an extensive comparison between data and a low-dimensional model of cellular physiology.
    1. Cell Biology
    2. Evolutionary Biology

    Epidermal threads reveal the origin of hagfish slime

    Yu Zeng, David C Plachetzki ... Douglas Fudge
    The hagfish slime glands, which produce a large volume of defensive slime within 0.4 s by ejecting threads and mucus into seawater, evolved from cells and genes expressed in the skin.
    1. Neuroscience

    Rab10 regulates the sorting of internalised TrkB for retrograde axonal transport

    Oscar Marcelo Lazo, Giampietro Schiavo
    BDNF-regulated deployment of Rab10 to the distal axon regulates retrograde propagation of neurotrophic signalling, providing a flexible mechanism to fine-tune the communication between the synapse and the cell body of neurons, as well as novel therapeutic targets for neurodegeneration.
    1. Structural Biology and Molecular Biophysics

    Cryo-EM structures of an LRRC8 chimera with native functional properties reveal heptameric assembly

    Hirohide Takahashi, Toshiki Yamada ... Erkan Karakas
    The cryo-electron microscopy structures of an LRRC8C chimera with native channel properties reveal the diverse oligomeric assembly and suggest that lipids may play a role in gating in volume-regulated anion channels.
    1. Plant Biology

    Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth

    Ivan Lebovka, Bruno Hay Mele ... Thomas Greb
    Radial plant growth produces large parts of terrestrial biomass and can be computationally simulated with the help of an instructive framework of intercellular communication loops.
    1. Cell Biology

    Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein

    Congyan Zhang, Randy Schekman
    A human endogenous fusogen, syncytin, which facilitates placental cell-cell fusion during early embryonic development, mediates the formation of open-ended membrane tubular connections between other cell types that efficiently promotes the intercellular transfer of cytoplasmic cargos including Cas9 protein and organelles.
    1. Developmental Biology

    MMP14 cleaves PTH1R in the chondrocyte-derived osteoblast lineage, curbing signaling intensity for proper bone anabolism

    Tsz Long Chu, Peikai Chen ... Kathryn Song Eng Cheah
    MMP14 activity-mediated cleavage of the ectodomain of PTH1R modulates PTH signaling in the chondrocyte-derived osteoblast lineage, regulating osteogenesis and thereby bone metabolism, with therapeutic significance for bone-wasting diseases.
    1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    Nuclear m6A reader YTHDC1 promotes muscle stem cell activation/proliferation by regulating mRNA splicing and nuclear export

    Yulong Qiao, Qiang Sun ... Huating Wang
    YTHDC1 is an essential factor controlling satellite cell regenerative ability through multifaceted gene regulatory mechanisms in myoblast cells.
    1. Genetics and Genomics

    Exploiting the mediating role of the metabolome to unravel transcript-to-phenotype associations

    Chiara Auwerx, Marie C Sadler ... Eleonora Porcu
    Integrating multi-omics quantitative trait loci and genome-wide association study data into a multivariable Mendelian randomization framework allows to identify metabolite-mediated transcript-to-phenotype causal relations missed by methods focusing on a single omics layer.
    1. Neuroscience

    Isolated catatonia-like executive dysfunction in mice with forebrain-specific loss of myelin integrity

    Sahab Arinrad, Constanze Depp ... Klaus-Armin Nave
    Loss of myelin integrity in the mouse forebrain perturbs executive functions, assessed by tests requiring normal motor performance.
    1. Cancer Biology
    2. Computational and Systems Biology

    Interplay of adherens junctions and matrix proteolysis determines the invasive pattern and growth of squamous cell carcinoma

    Takuya Kato, Robert P Jenkins ... Erik Sahai
    Cancer invasion occurs with different molecular and spatial patterns, thin invading strands are associated with mesenchymal cell features, such as low cell-cell adhesion, while broad strands are favoured for lymph node metastasis and require high matrix proteolysis and cell-cell adhesions.
    1. Neuroscience

    Effects of clozapine-N-oxide and compound 21 on sleep in laboratory mice

    Janine Traut, Jose Prius Mengual ... Lukas B Krone
    The two most commonly used chemogenetic actuators, clozapine-N-oxide and compound 21, similarly modulate sleep in wild-type laboratory mice indicating that even designer drugs which do not convert to clozapine can elicit behavioural effects and require adequate controls.
    1. Epidemiology and Global Health

    Associations of ABO and Rhesus D blood groups with phenome-wide disease incidence: A 41-year retrospective cohort study of 482,914 patients

    Peter Bruun-Rasmussen, Morten Hanefeld Dziegiel ... Søren Brunak
    The ABO and RhD blood groups are associated with disease-wide susceptibility differences.
    1. Cancer Biology
    2. Computational and Systems Biology

    Interrogating the precancerous evolution of pathway dysfunction in lung squamous cell carcinoma using XTABLE

    Matthew Roberts, Julia Ogden ... Carlos Lopez-Garcia
    XTABLE is the first easy-to-use bioinformatic solution that has been conceived and designed solely to interrogate the transcriptomes of premalignant stages of lung squamous cell carcinoma (LUSC) and enhance the development of LUSC prevention and detection strategies.
    1. Medicine
    2. Epidemiology and Global Health

    A systematic assessment of preclinical multilaboratory studies and a comparison to single laboratory studies

    Victoria T Hunniford, Agnes Grudniewicz ... Manoj M Lalu
    Preclinical multilaboratory studies are a robust method to assess promising interventions.
    1. Neuroscience

    Disease-modifying effects of sodium selenate in a model of drug-resistant, temporal lobe epilepsy

    Pablo M Casillas-Espinosa, Alison Anderson ... Terence J O'Brien
    Sodium selenate is the first treatment with persistent disease-modifying effects reducing seizures and improving cognitive deficits, in chronically epileptic and drug-resistant TLE animals.
    1. Neuroscience

    The integrated brain network that controls respiration

    Friedrich Krohn, Manuele Novello ... Laurens WJ Bosman
    The primary rhythm generators controlling respiration are integrated in a much larger network of brain regions, including areas not typically considered to be respiratory control centers, and that allow respiration to be adapted to all forms of ongoing behavior.
    1. Cancer Biology

    Kindlin-1 regulates IL-6 secretion and modulates the immune environment in breast cancer models

    Emily R Webb, Georgia L Dodd ... Valerie G Brunton
    New roles in anti-tumour immunity for the adhesion protein Kindlin-1 via regulation of cytokine secretion and T cell regulation.
    1. Biochemistry and Chemical Biology
    2. Cancer Biology

    Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells

    Madeleine L Hart, Evan Quon ... Lucas B Sullivan
    SDH-null cancer cells decrease mitochondrial complex I to drive rewired aspartate synthesis through reductive carboxylation and pyruvate carboxylase, supporting cell proliferation and tumor growth.
    1. Neuroscience

    High-resolution quantitative and functional MRI indicate lower myelination of thin and thick stripes in human secondary visual cortex

    Daniel Haenelt, Robert Trampel ... Nikolaus Weiskopf
    In vivo quantitative magnetic resonance imaging at ultra-high magnetic field reveals systematic differences of relaxation parameters (R1) within the human secondary visual cortex at the level of the thin-thick-pale stripes system, which points toward higher cortical myelination of pale stripes.
    1. Cancer Biology
    2. Computational and Systems Biology

    Pan-cancer association of DNA repair deficiencies with whole-genome mutational patterns

    Simon Grund Sørensen, Amruta Shrikhande ... Jakob Skou Pedersen
    Deficiencies of several DNA damage response genes can be predicted by their association with genome-wide mutation patterns.
    1. Neuroscience

    A process model account of the role of dopamine in intertemporal choice

    Alexander Soutschek, Philippe N Tobler
    Drift diffusion models suggest that dopamine affects dissociable components of the decision process in intertemporal choice, underlining the importance of analyzing the effects of neural interventions with process models.
    1. Cancer Biology

    Targeting the fatty acid binding proteins disrupts multiple myeloma cell cycle progression and MYC signaling

    Mariah Farrell, Heather Fairfield ... Michaela R Reagan
    The fatty acid binding protein (FABP) family of proteins plays a role in multiple myeloma cell survival and disease progression in vitro and in silico, and thus blocking the FABPs could have anti-cancer effects and prove beneficial for myeloma patients.
    1. Epidemiology and Global Health

    Reducing societal impacts of SARS-CoV-2 interventions through subnational implementation

    Mark M Dekker, Luc E Coffeng ... Sake J de Vlas
    An agent-based, population-scale, geographically explicit model, and integration of comprehensive data sources on demography, mobility, interactions and SARS-CoV-2 parameters, show that subnational implementations of intervention measures may provide better strategic choices for controlling future epidemics.
    1. Stem Cells and Regenerative Medicine

    Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo

    Dennis May, Sangwon Yun ... Valentina Greco
    Chromatin gradually rearranges during epidermal stem cell differentiation, while transcription of a differentiation-associated gene is extremely dynamic.
    1. Epidemiology and Global Health

    The effect of variation of individual infectiousness on SARS-CoV-2 transmission in households

    Tim K Tsang, Xiaotong Huang ... Benjamin John Cowling
    Household transmission modeling qualified variation of individual infectiousness among infected persons, which could be caused by both biological factors and host behaviors.
    1. Neuroscience

    Evolution of neural activity in circuits bridging sensory and abstract knowledge

    Francesca Mastrogiuseppe, Naoki Hiratani, Peter Latham
    Gradient-descent synaptic plasticity applied to neuroscience categorization tasks captures the behaviour of common neural activity measures (category and context selectivity, correlation, asymmetry) reported in experiments, and makes novel experimental predictions.
    1. Structural Biology and Molecular Biophysics

    Room-temperature crystallography reveals altered binding of small-molecule fragments to PTP1B

    Tamar Skaist Mehlman, Justin T Biel ... Daniel A Keedy
    Many small-molecule fragments bind differently to the allosteric protein PTP1B in room-temperature instead of cryogenic crystal structures, which may be relevant for structure-based drug design.
    1. Genetics and Genomics

    Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency

    Bryn D Webb, Sara M Nowinski ... Sander M Houten
    Biallelic variants in MCAT are associated with combined oxidative phosphorylation deficiency in humans and a clinical presentation that includes hypotonia, developmental delay, failure to thrive, and nystagmus.
    1. Genetics and Genomics

    The landscape of m1A modification and its posttranscriptional regulatory functions in primary neurons

    Chi Zhang, Xianfu Yi ... Hengxing Zhou
    The m1A modification plays vital roles in the regulation of the biological functions of multiple RNA species (including mRNA, lncRNA, and circRNA) and influences the interactions between these RNAs.
    1. Microbiology and Infectious Disease

    Antibiotic-induced accumulation of lipid II synergizes with antimicrobial fatty acids to eradicate bacterial populations

    Ashelyn E Sidders, Katarzyna M Kedziora ... Brian P Conlon
    Palmitoleic acid not only potentiates vancomycin killing of Gram-positive bacteria, but the insertion of palmitoleic acid into the membrane leads to the unexpected accumulation of membrane-bound cell wall precursors, representing a novel mechanism of action for unsaturated fatty acids.
    1. Stem Cells and Regenerative Medicine

    Identification of phenotypically, functionally, and anatomically distinct stromal niche populations in human bone marrow based on single-cell RNA sequencing

    Hongzhe Li, Sandro Bräunig ... Stefan Scheding
    Single-cell transcriptomic profiling of human bone marrow non-hematopoietic cells provides the basis for a comprehensive understanding of the cellular complexity of the hematopoietic stem cell (HSC) microenvironment and the intricate stroma-hematopoiesis crosstalk mechanisms.
    1. Cell Biology
    2. Cancer Biology

    Transferred mitochondria accumulate reactive oxygen species, promoting proliferation

    Chelsea U Kidwell, Joseph R Casalini ... Minna Roh-Johnson
    Transferred mitochondria do not always regulate cell behavior by simply restoring mitochondrial function, but rather, transferred mitochondria are dysfunctional, initiating downstream signaling and proliferation in response to reactive oxygen species.
    1. Structural Biology and Molecular Biophysics

    Structural insight into the stabilization of microtubules by taxanes

    Andrea E Prota, Daniel Lucena-Agell ... J Fernando Díaz
    Crystal structures of taxane-tubulin complexes allow the understanding of the interaction of paclitaxel (Taxol) with its cellular target in atomic detail and assess the structural determinants for binding.
    1. Neuroscience

    Distinct roles of forward and backward alpha-band waves in spatial visual attention

    Andrea Alamia, Lucie Terral ... Rufin VanRullen
    Covert visual attention modulates alpha-band traveling waves propagating from frontal to occipital regions in both hemispheres, yet it modulates waves propagating in the opposite direction (occipital to frontal) only in the presence of visual stimulation.
    1. Cell Biology
    2. Genetics and Genomics

    Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function

    Mi Huang, Daniel Coral ... Sebastian Kalamajski
    MTIF3 safeguards mitochondrial electron transport chain assembly in adipocytes, and affects thus adipocyte fatty acid oxidation ability, which can explain why MTIF3 genetic variation can influence dietary intervention aimed at weight loss.
    1. Chromosomes and Gene Expression
    2. Developmental Biology

    The long noncoding RNA Charme supervises cardiomyocyte maturation by controlling cell differentiation programs in the developing heart

    Valeria Taliani, Giulia Buonaiuto ... Monica Ballarino
    The lncRNA pCharme controls the expression of cardiomyocyte maturation genes and heart development by orchestrating the formation of MATR3-enriched nuclear condensates.
    1. Neuroscience

    Experience-dependent flexibility in a molecularly diverse central-to-peripheral auditory feedback system

    Michelle M Frank, Austen A Sitko ... Lisa V Goodrich
    Single-nucleus sequencing, anatomy, and physiology reveal heterogeneity among olivocochlear neurons (a group of cells that provide feedback to the inner ear), identify a neuropeptide-enriched subtype, and show that neuropeptide expression changes during postnatal development and after sound exposure.
    1. Developmental Biology
    2. Physics of Living Systems

    Cellular compartmentalisation and receptor promiscuity as a strategy for accurate and robust inference of position during morphogenesis

    Krishnan S Iyer, Chaitra Prabhakara ... Madan Rao
    An information theoretic and systems biology approach shows that cellular compartmentalisation and receptor promiscuity can facilitate the accurate and robust inference of position from noisy morphogen profiles, verified by experiments in Drosophila wing imaginal disc.
    1. Neuroscience

    Contrary neuronal recalibration in different multisensory cortical areas

    Fu Zeng, Adam Zaidel, Aihua Chen
    Single-unit recordings from cortical neurons in behaving macaque monkeys expose differential aspects of multisensory plasticity across different multisensory areas during visual–vestibular recalibration.
    1. Stem Cells and Regenerative Medicine

    Hepatic lipid overload triggers biliary epithelial cell activation via E2Fs

    Ece Yildiz, Gaby El Alam ... Kristina Schoonjans
    Chronic fat overload triggers the conversion of quiescent biliary epithelial cells into proliferative progenitors through an E2F-dependent mechanism.
    1. Cell Biology

    Enhanced single RNA imaging reveals dynamic gene expression in live animals

    Yucen Hu, Jingxiu Xu ... Weirui Ma
    The combination of the MS2 system with the Suntag system as a signal amplifier allows enhanced single-molecule mRNA imaging in live cells and animals.
    1. Neuroscience

    Molecular and spatial profiling of the paraventricular nucleus of the thalamus

    Claire Gao, Chiraag A Gohel ... Mario A Penzo
    Molecular and spatial profiling methods idenified five neuronal subtypes that are distributed along the anteroposterior axis of the paraventricular thalamus, a structure increasingly implicated in the control of emotional and motivated behaviors.
    1. Developmental Biology

    A gene regulatory network for neural induction

    Katherine E Trevers, Hui-Chun Lu ... Claudio D Stern
    A comprehensive resource based on analysis of the responses of embryonic ectoderm cells to signals from the 'organizer' (the node) including transcriptional responses and epigenetic changes with fine temporal dynamics, predicted regulatory interactions, and conservation among vertebrates.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    RNA localization mechanisms transcend cell morphology

    Raeann Goering, Ankita Arora ... J Matthew Taliaferro
    Mechanisms that regulate RNA localization in one cell type predictably regulate RNA localization in other cell types, even if they have vastly different morphologies, implying an underlying regulatory code that cuts across specific subcellular structures.
    1. Biochemistry and Chemical Biology
    2. Developmental Biology

    O-GlcNAc glycosylation orchestrates fate decision and niche function of bone marrow stromal progenitors

    Zengdi Zhang, Zan Huang ... Hai-Bin Ruan
    OGT-mediated protein O-GlcNAcylation balances osteogenic versus adipogenic differentiation and controls hematopoietic niche function of bone marrow stromal cells.
    1. Cell Biology
    2. Immunology and Inflammation

    Single-cell analysis reveals dynamics of human B cell differentiation and identifies novel B and antibody-secreting cell intermediates

    Niels JM Verstegen, Sabrina Pollastro ... S Marieke van Ham
    Single-cell RNA sequencing provides a detailed understanding of the cues controlling in vitro differentiation of human B cells into antibody-secreting cells (ASCs), uncovering a novel pre-ASC population present ex vivo in lymphoid tissues that progress through a germinal center-like state.
    1. Genetics and Genomics

    Quantitative proteomic analysis of skeletal muscles from wild-type and transgenic mice carrying recessive Ryr1 mutations linked to congenital myopathies

    Jan Eckhardt, Alexis Ruiz ... Francesco Zorzato
    Quantitative proteomic analysis shows that recessive Ryr1 mutations not only decrease the content of RyR1 protein in muscle, but also affect the content of many other proteins involved in a variety of biological processes.
    1. Neuroscience

    The entorhinal-DG/CA3 pathway in the medial temporal lobe retains visual working memory of a simple surface feature

    Weizhen Xie, Marcus Cappiello ... Weiwei Zhang
    High-resolution fMRI data reveal an often-neglected contribution of the medial temporal lobe circuitry to item-specific representation in visual working memory, suggesting the mechanism traditionally deemed dedicated to long-term memory can be exploited to support the quality of human working memory.
    1. Cell Biology
    2. Neuroscience

    Large vesicle extrusions from C. elegans neurons are consumed and stimulated by glial-like phagocytosis activity of the neighboring cell

    Yu Wang, Meghan Lee Arnold ... Barth D Grant
    Giant neuron derived vesicles called exophers are processed by phagocytosis in neighboring skin cells, and the phagocytic interaction promotes exopher production.
    1. Evolutionary Biology

    The layered costs and benefits of translational redundancy

    Parth K Raval, Wing Yui Ngan ... Deepa Agashe
    Manipulation of the E. coli translation machinery sheds new light on evolutionary constraints and optimization strategies for bacterial translation and growth.
    1. Microbiology and Infectious Disease

    Germinal center B cells that acquire nuclear proteins are specifically suppressed by follicular regulatory T cells

    Fang Ke, Zachary L Benet ... Irina L Grigorova
    Targeting nuclear self-antigens to germinal center B cells induces rapid accumulation of follicular regulatory T cells with immunosuppressive phenotype and leads to inhibition of germinal center response with predominant suppression of the nuclear protein-acquiring germinal center B cells.
    1. Structural Biology and Molecular Biophysics

    Peptides that Mimic RS repeats modulate phase separation of SRSF1, revealing a reliance on combined stacking and electrostatic interactions

    Talia Fargason, Naiduwadura Ivon Upekala De Silva ... Jun Zhang
    Short peptides that mimic the regions responsible for phase separation can be used to solubilize phase-separating proteins in their native states and to determine the mechanism of phase separation.
    1. Structural Biology and Molecular Biophysics

    Conformational and oligomeric states of SPOP from small-angle X-ray scattering and molecular dynamics simulations

    F Emil Thomasen, Matthew J Cuneo ... Kresten Lindorff-Larsen
    Self-association of speckle-type POZ protein (SPOP), a substrate adaptor in the ubiquitin proteasome system, is studied by combining small-angle X-ray scattering and molecular dynamics simulations to reveal the structure of the protein assemblies in solution.
    1. Structural Biology and Molecular Biophysics

    Conserved allosteric inhibition mechanism in SLC1 transporters

    Yang Dong, Jiali Wang ... Christof Grewer
    Functional and computational studies reveal that the allosteric inhibition mechanism is conserved between glutamate and neutral amino acid transporters of the SLC1 family, and identify a novel allosteric inhibitor.
    1. Developmental Biology
    2. Physics of Living Systems

    Controlling periodic long-range signalling to drive a morphogenetic transition

    Hugh Z Ford, Angelika Manhart, Jonathan R Chubb
    Cells control signal circulation to set the frequency of information transmitted over tissue scales.
    1. Plant Biology

    RPG acts as a central determinant for infectosome formation and cellular polarization during intracellular rhizobial infections

    Beatrice Lace, Chao Su ... Thomas Ott
    Infectosomes enabling polar progression of infection threads in legume roots require the symbioisis-related RPG protein that functions as an organizer of these structures.