February 2021

Research articles

1. 1. Microbiology and Infectious Disease

   MetaHiC phage-bacteria infection network reveals active cycling phages of the healthy human gut

   Martial Marbouty, Agnès Thierry ... Romain Koszul

   Physical contacts of bacteria and phage genomes allow in situ exploration of underdocumented phage families in the gut.
2. 1. Computational and Systems Biology
   2. Ecology

   TRex, a fast multi-animal tracking system with markerless identification, and 2D estimation of posture and visual fields

   Tristan Walter, Iain D Couzin

   Being fast, memory efficient, easy to use, and with powerful integrated tools, TRex will lower barriers of entry into, and enable more ambitious approaches to, the quantitative study of behavior.
3. 1. Genetics and Genomics

   DNA methylation meta-analysis reveals cellular alterations in psychosis and markers of treatment-resistant schizophrenia

   Eilis Hannon, Emma L Dempster ... Jonathan Mill

   DNA methylation data can be harnessed to provide insights into molecular and phenotypic differences associated with the spectrum of psychosis diagnoses.
4. 1. Neuroscience

   Brain endothelial cell TRPA1 channels initiate neurovascular coupling

   Pratish Thakore, Michael G Alvarado ... Scott Earley

   Activation of TRPA1 channels on cerebral capillary endothelial cells initiates a retrograde signal that propagates and dilates upstream arterioles.
5. 1. Evolutionary Biology
   2. Microbiology and Infectious Disease

   Integron activity accelerates the evolution of antibiotic resistance

   Célia Souque, José Antonio Escudero, R Craig MacLean

   Integrons deploy a variety of adaptive strategies including excision, shuffling, and duplication of cassettes that foster rapid bacterial adaptation and resistance evolution while protecting the genomic integrity of the host.
6. 1. Physics of Living Systems
   2. Stem Cells and Regenerative Medicine

   DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome

   Simona Hankeova, Jakub Salplachta ... Emma Rachel Andersson

   Dual resin casting of multiple lumenized systems followed by microCT allows visualization, digitalization, and quantification of architectural parameters defining the vascular and biliary systems in liver revealing new phenotypes.
7. 1. Biochemistry and Chemical Biology

   Identification of host proteins differentially associated with HIV-1 RNA splice variants

   Rachel Knoener, Edward Evans III ... Lloyd M Smith

   The distinct protein-RNA interactomes of HIV-1 RNA splice forms are revealed using a powerful multiplex strategy for RNA capture and mass spectrometric analysis.
8. 1. Plant Biology

   A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis

   Rosa Pipitone, Simona Eicke ... Emilie Demarsy

   Serial-Block-Face Scanning Electron Microscopy (SBF-SEM) associated with biomolecular analysis show that chloroplast differentiation proceeds by distinct ‘structure establishment’ and ‘chloroplast proliferation’ phases, each with differential protein and lipid regulation.
9. 1. Cell Biology

   The SWELL1-LRRC8 complex regulates endothelial AKT-eNOS signaling and vascular function

   Ahmad F Alghanem, Javier Abello ... Rajan Sah

   SWELL1 is required for basal, stretch, and flow-mediated endothelial AKT-eNOS signaling in vitro and protects against angiotensin-induced hypertension and diabetes-associated vascular dysfunction in vivo.
10. 1. Cell Biology
    2. Developmental Biology

    A Non-stop identity complex (NIC) supervises enterocyte identity and protects from premature aging

    Neta Erez, Lena Israitel ... Amir Orian

    A nuclear Non-stop identity complex (NIC) supervises enterocyte (EC) identity, preserves tissue homeostasis, and prevents premature aging by maintaining EC-specific gene expression, silencing non-relevant programs, and safeguarding large-scale nuclear organization.
11. 1. Biochemistry and Chemical Biology

    Real-time monitoring of peptidoglycan synthesis by membrane-reconstituted penicillin-binding proteins

    Víctor M Hernández-Rocamora, Natalia Baranova ... Waldemar Vollmer

    The synthesis of bacterial cell wall peptidoglycan was reconstituted in lipid bilayers and detected by a novel Förster resonance energy transfer real-time assay.
12. 1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Glycine acylation and trafficking of a new class of bacterial lipoprotein by a composite secretion system

    Christopher Icke, Freya J Hodges ... Ian R Henderson

    AatD is a novel acyltransferase that mediates N-palmitoylation of glycine, a process not previously described, that results in the secretion of lipoproteins to the bacterial cell surface.
13. 1. Neuroscience

    Specialized contributions of mid-tier stages of dorsal and ventral pathways to stereoscopic processing in macaque

    Toshihide W Yoshioka, Takahiro Doi ... Ichiro Fujita

    Visual area MT signals binocular disparity quickly and robustly, whereas area V4 or its upstream area transforms the nature of the signals to derive a more sophisticated depth representation.
14. 1. Computational and Systems Biology
    2. Neuroscience

    Graphical-model framework for automated annotation of cell identities in dense cellular images

    Shivesh Chaudhary, Sol Ah Lee ... Hang Lu

    Unbiased and automatic annotation using structured prediction framework with efficiently built data-driven atlases is more accurate than registration-based methods for cell identifications in dense images and enables fast whole-brain analysis.
15. 1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    Viral load and contact heterogeneity predict SARS-CoV-2 transmission and super-spreading events

    Ashish Goyal, Daniel B Reeves ... Bryan T Mayer

    A mathematical model was used to establish a simple conceptual basis for why super-spreader events fundamentally drive the spread of SARS-CoV-2 but not influenza.
16. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    A nuclease- and bisulfite-based strategy captures strand-specific R-loops genome-wide

    Phillip Wulfridge, Kavitha Sarma

    BisMapR reveals strand-specific R-loops with high resolution at small-scale genomic features including bidirectionally transcribed promoters and enhancers.
17. 1. Epidemiology and Global Health
    2. Evolutionary Biology

    COVID-19 CG enables SARS-CoV-2 mutation and lineage tracking by locations and dates of interest

    Albert Tian Chen, Kevin Altschuler ... Benjamin E Deverman

    COVID-19 CG is an open source web resource that allows vaccine, therapeutics, and diagnostics developers to track SARS-CoV-2 mutations and lineages by location and time.
18. 1. Biochemistry and Chemical Biology
    2. Genetics and Genomics

    A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch

    Hélène Duplus-Bottin, Martin Spichty ... Gaël Yvert

    A single protein catalyzes site-specific DNA recombination upon blue-light illumination.
19. 1. Cancer Biology

    Dissecting phenotypic transitions in metastatic disease via photoconversion-based isolation

    Yogev Sela, Jinyang Li ... Ben Z Stanger

    A photoconversion-based isolation technique enables systematic investigation of spatially distinct small metastases (microcolonies) versus large lesions (macrometastases).
20. 1. Developmental Biology

    Three-dimensional reconstruction of a whole insect reveals its phloem sap-sucking mechanism at nano-resolution

    Xin-Qiu Wang, Jian-sheng Guo ... Chuan-Xi Zhang

    3D reconstruction for an insect provides new internal structures at nanometer resolution, and the reconstructed feeding insect reveals unexpected contraction of stylet protractors and suggested a novel phloem sap-sucking model.
21. 1. Developmental Biology

    Deficient spermiogenesis in mice lacking Rlim

    Feng Wang, Maria Gracia Gervasi ... Ingolf Bach

    Genetic analyses in mice identifies Rlim in the spermatogenic cell lineage as a regulator of cytoplasmic reduction during spermiogenesis.
22. 1. Computational and Systems Biology

    Shape deformation analysis reveals the temporal dynamics of cell-type-specific homeostatic and pathogenic responses to mutant huntingtin

    Lucile Megret, Barbara Gris ... Christian Neri

    Cell-type-specific molecular systems mapping reveals that striatal neuron degeneration in Huntington's disease is primarily driven by the loss of homeostatic responses.
23. 1. Cell Biology
    2. Developmental Biology

    Physically asymmetric division of the C. elegans zygote ensures invariably successful embryogenesis

    Radek Jankele, Rob Jelier, Pierre Gönczy

    Systematic analysis of C. elegans zygotes manipulated to divide equally demonstrates that daughter cell size asymmetry is critical for proper cell cycle timing, positioning, and fates during subsequent embryogenesis.
24. 1. Developmental Biology
    2. Neuroscience

    Exocyst-mediated membrane trafficking of the lissencephaly-associated ECM receptor dystroglycan is required for proper brain compartmentalization

    Andriy S Yatsenko, Mariya M Kucherenko ... Halyna R Shcherbata

    Neuronal interacting proteome reveals that the cellular dynamics of the lissencephaly-associated extracellular matrix receptor dystroglycan are governed by the exocyst complex, which is key for proper brain assembly.
25. 1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Molecular basis for functional connectivity between the voltage sensor and the selectivity filter gate in Shaker K⁺ channels

    Carlos AZ Bassetto, João Luis Carvalho-de-Souza, Francisco Bezanilla

    A chain of residues connecting the voltage sensing domain and the pore domain is responsible for a noncanonical communication between these domains.
26. 1. Neuroscience

    Incomplete removal of extracellular glutamate controls synaptic transmission and integration at a cerebellar synapse

    Timothy S Balmer, Carolina Borges-Merjane, Laurence O Trussell

    Extracellular levels of the neurotransmitter glutamate are maintained at physiologically significant levels at some synapses even in the absence of action potentials.
27. 1. Immunology and Inflammation

    Dichloroacetate reverses sepsis-induced hepatic metabolic dysfunction

    Rabina Mainali, Manal Zabalawi ... Matthew A Quinn

    Integrative multi-omics reveals global dysregulation in hepatic metabolism during prolonged sepsis.
28. 1. Genetics and Genomics
    2. Neuroscience

    Investigation of Drosophila fruitless neurons that express Dpr/DIP cell adhesion molecules

    Savannah G Brovero, Julia C Fortier ... Michelle N Arbeitman

    Analyses of fru P1 neurons that express dpr/DIP genes reveal roles for subsets of neurons in courtship, and the roles of Dpr/DIPs and the sex hierarchy in neuroanatomical sexual dimorphism.
29. 1. Neuroscience

    Evolutionary shifts in taste coding in the fruit pest Drosophila suzukii

    Hany KM Dweck, Gaëlle JS Talross ... John R Carlson

    The evolutionary transition of the agricultural pest Drosophila suzukii to egg laying on ripe fruits was paralleled with several gustatory innovations.
30. 1. Biochemistry and Chemical Biology
    2. Immunology and Inflammation

    Integration of IL-2 and IL-4 signals coordinates divergent regulatory T cell responses and drives therapeutic efficacy

    Julie Y Zhou, Carlos A Alvarez, Brian A Cobb

    Simultaneous cytokine signaling results in unexpected transcription factor changes that fuel a cellular response divergent from the sum of each cytokine alone.
31. 1. Neuroscience

    Functional reallocation of sensory processing resources caused by long-term neural adaptation to altered optics

    Antoine Barbot, Woon Ju Park ... Geunyoung Yoon

    Chronic exposure to poor optical quality causes a functional reallocation of sensory processing resources that favors perceptual information less affected by the eye's optics.
32. 1. Neuroscience

    Accelerating with FlyBrainLab the discovery of the functional logic of the Drosophila brain in the connectomic and synaptomic era

    Aurel A Lazar, Tingkai Liu ... Yiyin Zhou

    FlyBrainLab is an open-source computing platform that integrates 3D exploration and visualization of diverse Drosophila connectomic/synaptomic datasets with interactive exploration of the functional logic of modeled executable brain circuits.
33. 1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs, and mRNAs in live bacterial cells

    Seongjin Park, Karine Prévost ... Jingyi Fei

    RNA chaperone Hfq dynamically binds various pools of cellular RNAs to execute different regulatory functions.
34. 1. Cell Biology
    2. Chromosomes and Gene Expression

    DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7

    Lorraine De Jesús-Kim, Larry J Friedman ... Stephen P Bell

    A multi-step process of helicase activation sensitizes replication initiation to the extent of replicative helicase phosphorylation.
35. 1. Cell Biology
    2. Computational and Systems Biology

    Identifying molecular features that are associated with biological function of intrinsically disordered protein regions

    Taraneh Zarin, Bob Strome ... Alan M Moses

    A statistical model systematically associates functions of intrinsically disordered regions with sequence-distributed molecular features such as charge, residue composition, or repeat content.
36. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Myosin with hypertrophic cardiac mutation R712L has a decreased working stroke which is rescued by omecamtiv mecarbil

    Aaron Snoberger, Bipasha Barua ... E Michael Ostap

    Mechanochemical defects of a β-cardiac myosin mutation that results in severe hypertrophic cardiomyopathy are reported and the heart failure drug omecamtiv mecarbil rescues these defects.
37. 1. Cell Biology

    LAP2alpha maintains a mobile and low assembly state of A-type lamins in the nuclear interior

    Nana Naetar, Konstantina Georgiou ... Roland Foisner

    The lamin A/C binding protein LAP2α inhibits formation of higher order lamin structures in the nuclear interior in a lamin A/C-phosphorylation-independent manner, thereby regulating chromatin mobility.
38. 1. Structural Biology and Molecular Biophysics

    Molecular structures of the eukaryotic retinal importer ABCA4

    Fangyu Liu, James Lee, Jue Chen

    The structures of ABCA4 in two conformations provide a framework to understand its transport mechanism.
39. 1. Ecology

    A remote sensing derived data set of 100 million individual tree crowns for the National Ecological Observatory Network

    Ben G Weinstein, Sergio Marconi ... Ethan P White

    A first continental data set of tree crown predictions is derived across the United States for the National Ecological Observatory Network.
40. 1. Microbiology and Infectious Disease

    Redox controls RecA protein activity via reversible oxidation of its methionine residues

    Camille Henry, Laurent Loiseau ... Benjamin Ezraty

    RecA is inactivated by oxidative stress and repaired by the MsrA/B anti-ROS defense system.
41. 1. Ecology

    Volatile DMNT directly protects plants against Plutella xylostella by disrupting the peritrophic matrix barrier in insect midgut

    Chen Chen, Hongyi Chen ... Peijin Li

    The natural volatile component DMNT protects plants from insect attacks by damaging peritropic matrix barriers in insect midguts, paving a promising way for the molecular breeding of plant protection.
42. 1. Chromosomes and Gene Expression

    Short-term exposure to intermittent hypoxia leads to changes in gene expression seen in chronic pulmonary disease

    Gang Wu, Yin Yeng Lee ... David F Smith

    RNA profiles from lungs of mice exposed to intermittent hypoxia shared similarity with gene expression changes in human lung from patients with pulmonary diseases, including pulmonary hypertension, COPD, and asthma.
43. 1. Neuroscience

    Medium spiny neurons activity reveals the discrete segregation of mouse dorsal striatum

    Javier Alegre-Cortés, María Sáez ... Ramon Reig

    Analysis of slow wave brain state unravels the functional connectivity and the biological substrate of the rodent dorsolateral and dorsomedial striatum, demonstrating its organization in two non-overlapping circuits.
44. 1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Virus infection of the CNS disrupts the immune-neural-synaptic axis via induction of pleiotropic gene regulation of host responses

    Olga A Maximova, Daniel E Sturdevant ... Alexander G Pletnev

    Virus infection of the central nervous system disrupts the homeostasis of the immune-neural-synaptic axis via induction of pleiotropic genes with an unintended off-target negative impact on the neurotransmission.
45. 1. Neuroscience

    Control of feeding by Piezo-mediated gut mechanosensation in Drosophila

    Soohong Min, Yangkyun Oh ... Stephen Liberles

    Gut-innervating sensory neurons detect large food volumes and inhibit feeding in Drosophila through the mechanically gated ion channel Piezo.
46. 1. Cell Biology
    2. Chromosomes and Gene Expression

    Repressing Ago2 mRNA translation by Trim71 maintains pluripotency through inhibiting let-7 microRNAs

    Qiuying Liu, Xiaoli Chen ... Wenqian Hu

    Repressing the conserved pro-differentiation let-7 microRNAs through limiting Ago2 levels is critical to maintaining pluripotency in stem cells.
47. 1. Evolutionary Biology
    2. Genetics and Genomics

    High-quality carnivoran genomes from roadkill samples enable comparative species delineation in aardwolf and bat-eared fox

    Rémi Allio, Marie-Ka Tilak ... Frédéric Delsuc

    High-quality genomes can be obtained from roadkill samples and used in population genomics for species delimitation with conservation implications.
48. 1. Immunology and Inflammation

    The kinase PDK1 is critical for promoting T follicular helper cell differentiation

    Zhen Sun, Yingpeng Yao ... Shuyang Yu

    PDK1 serves as a critical role for intrinsically promoting T follicular helper cell differentiation and effector functions via controlling expression level of TCF1.
49. 1. Chromosomes and Gene Expression
    2. Neuroscience

    HAT cofactor TRRAP modulates microtubule dynamics via SP1 signaling to prevent neurodegeneration

    Alicia Tapias, David Lázaro ... Zhao-Qi Wang

    The Trrap-HAT-Sp1 axis operates a conserved transcriptional program to control proper microtubule dynamics in brain homeostasis and prevents neurodegeneration.
50. 1. Ecology
    2. Microbiology and Infectious Disease

    Three-dimensional biofilm colony growth supports a mutualism involving matrix and nutrient sharing

    Heidi A Arjes, Lisa Willis ... Kerwyn Casey Huang

    Novel high-throughput co-culture assays reveal how the spatial structure of colony biofilms provides opportunities for nutrient and matrix sharing and may drive the evolution of cooperative behaviors.
51. 1. Neuroscience

    The temporal and spectral characteristics of expectations and prediction errors in pain and thermoception

    Andreas Strube, Michael Rose ... Christian Büchel

    Key variables required for pain perception in the context of a predictive coding model are correlated with distinct oscillatory profiles.
52. 1. Neuroscience

    Exposing distinct subcortical components of the auditory brainstem response evoked by continuous naturalistic speech

    Melissa J Polonenko, Ross K Maddox

    The response from discrete stages of the early auditory pathway can be measured by subtle manipulations to long-form natural speech stimuli paired with deconvolution analysis of electroencephalography data.
53. 1. Genetics and Genomics

    Divergence in alternative polyadenylation contributes to gene regulatory differences between humans and chimpanzees

    Briana E Mittleman, Sebastian Pott ... Yoav Gilad

    A comparative analysis of human and chimpanzee polyadenylation site usage establishes alternative polyadenylation as another key mechanism underlying the genetic regulation of transcript and protein expression levels in primates.
54. 1. Physics of Living Systems

    Theory for the optimal detection of time-varying signals in cellular sensing systems

    Giulia Malaguti, Pieter Rein ten Wolde

    A theory is presented for the optimal design of cellular sensing systems that maximizes the sensing precision given resource constraints.
55. 1. Cell Biology

    One-shot analysis of translated mammalian lncRNAs with AHARIBO

    Luca Minati, Claudia Firrito ... Massimiliano Clamer

    Detection of translated lncRNAs through selective isolation of active ribosomes, ribosome-associated RNAs and corresponding de novo synthesised peptides.
56. 1. Chromosomes and Gene Expression

    Cohesin architecture and clustering in vivo

    Siheng Xiang, Douglas Koshland

    Cohesin adopts a flexible butterfly conformation in vivo and forms spatial and temporal regulated ordered clusters to maintain cohesion and condensation.
57. 1. Developmental Biology

    Differential activation of JAK-STAT signaling reveals functional compartmentalization in Drosophila blood progenitors

    Diana Rodrigues, Yoan Renaud ... Maneesha S Inamdar

    Comprehensive developmental in situ analysis of the Drosophila lymph gland provides markers highlighting blood progenitor diversity and reveals that JAK-STAT signaling prevents posterior progenitor differentiation, promoting survival after immune challenge.
58. 1. Epidemiology and Global Health

    Antimicrobial resistance and COVID-19: Intersections and implications

    Gwenan M Knight, Rebecca E Glover ... Clare IR Chandler

    COVID-19 will have an ongoing impact on antimicrobial resistance acquisition, transmission, and burden, requiring the close attention of researchers globally to generate a complete evidence base for the shifted dynamics.
59. 1. Biochemistry and Chemical Biology
    2. Evolutionary Biology

    Mitochondrial copper and phosphate transporter specificity was defined early in the evolution of eukaryotes

    Xinyu Zhu, Aren Boulet ... Paul A Cobine

    Ancient evolutionary signatures reveal mechanistic details of substrate specificity in copper-transporting mitochondria carrier family proteins.
60. 1. Computational and Systems Biology
    2. Medicine

    Individual variation in Achilles tendon morphology and geometry changes susceptibility to injury

    Nai-Hao Yin, Paul Fromme ... Helen L Birch

    Studying individual Achilles tendon geometry and interface sliding capacity may allow prediction of injury sites, and targeted training on specific muscle-(sub-)tendon units may boost beneficial outcomes for Achilles tendinopathy.
61. 1. Microbiology and Infectious Disease
    2. Plant Biology

    Proximity proteomics in a marine diatom reveals a putative cell surface-to-chloroplast iron trafficking pathway

    Jernej Turnšek, John K Brunson ... Andrew Ellis Allen

    An iron-sensitive gene cluster encodes proteins that co-localize with phytotransferrin endosomes and are involved in key intracellular iron transformation and trafficking processes in a model marine diatom.
62. 1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Structural basis of Stu2 recruitment to yeast kinetochores

    Jacob A Zahm, Michael G Stewart ... Matthew P Miller

    The results here show that Stu2 binds kinetochores by associating with the Ndc80 complex and that the interaction is critical for accurate chromosome segregation during cell division.
63. 1. Developmental Biology
    2. Neuroscience

    Olig3 regulates early cerebellar development

    Elijah D Lowenstein, Aleksandra Rusanova ... Luis R Hernandez-Miranda

    The transcription factor Olig3 safeguards the correct specification of early born cerebellar neuron derivatives and curtails an inhibitory interneuron differentiation program.
64. 1. Cell Biology

    In vivo proteomic mapping through GFP-directed proximity-dependent biotin labelling in zebrafish

    Zherui Xiong, Harriet P Lo ... Thomas E Hall

    BLITZ system enables proximity-dependent biotin labelling in live zebrafish embryos with cell and tissue specificity, providing a versatile and valuable tool for proteomic discovery using the zebrafish model.
65. 1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    A paucigranulocytic asthma host environment promotes the emergence of virulent influenza viral variants

    Katina D Hulme, Anjana C Karawita ... Kirsty R Short

    Patients with paucigranulocytic asthma may be more susceptible to severe influenza and could potentially be source of new, more virulent, influenza virus variants.
66. 1. Structural Biology and Molecular Biophysics

    A critical residue in the α₁M2–M3 linker regulating mammalian GABA_A receptor pore gating by diazepam

    Joseph W Nors, Shipra Gupta, Marcel P Goldschen-Ohm

    The role of an important gating domain in linkage between the benzodiazepine drug-binding site and the pore gate in GABA_A receptors.
67. 1. Neuroscience

    A spinoparabrachial circuit defined by Tacr1 expression drives pain

    Arnab Barik, Anupama Sathyamurthy ... Alexander Chesler

    A circuit involving Tacr1-expressing neurons in the spinal cord and the parabrachial nucleus controls how mice respond to a wide range of persistently painful stimuli.
68. 1. Developmental Biology

    An atlas of neural crest lineages along the posterior developing zebrafish at single-cell resolution

    Aubrey GA Howard IV, Phillip A Baker ... Rosa A Uribe

    Single-cell dissection of recent neural crest derivatives in the vertebrate zebrafish reveals diverse transcriptomic signatures among differentiating posterior cell types during the embryonic to larval stage transition.
69. 1. Developmental Biology
    2. Medicine

    Early postnatal interactions between beige adipocytes and sympathetic neurites regulate innervation of subcutaneous fat

    Jingyi Chi, Zeran Lin ... Paul Cohen

    The regulation of sympathetic innervation density by beige adipocytes in subcutaneous fat is important during a critical developmental window, but dispensable during adulthood.
70. 1. Microbiology and Infectious Disease

    BipA exerts temperature-dependent translational control of biofilm-associated colony morphology in Vibrio cholerae

    Teresa del Peso Santos, Laura Alvarez ... Felipe Cava

    Vibrio cholerae uses a conserved ribosome assembly factor to repress biofilm formation at low temperatures.
71. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    SNPC-1.3 is a sex-specific transcription factor that drives male piRNA expression in C. elegans

    Charlotte P Choi, Rebecca J Tay ... John K Kim

    snpc-1.3 specifies male piRNA transcription and is regulated by the sex determination pathway.
72. 1. Neuroscience

    Temporo-parietal cortex involved in modeling one’s own and others’ attention

    Arvid Guterstam, Branden J Bio ... Michael Graziano

    Functional brain scans of human participants show that the brain encodes other people's attention in enough richness to distinguish whether that attention was directed exogenously (stimulus-driven) or endogenously (internally driven).
73. 1. Cell Biology

    Bardet–Biedl syndrome 3 protein promotes ciliary exit of the signaling protein phospholipase D via the BBSome

    Yan-Xia Liu, Bin Xue ... Zhen-Chuan Fan

    Bardet–Biedl syndrome 3 (BBS3) protein promotes phospholipase D to load onto the BBSome at the ciliary tip for moving out of the cilium via intraflagellar transport.
74. 1. Biochemistry and Chemical Biology
    2. Neuroscience

    Phasic oxygen dynamics confounds fast choline-sensitive biosensor signals in the brain of behaving rodents

    Ricardo M Santos, Anton Sirota

    Fast fluctuations of oxygen, linked to behavioral and neural dynamics in vivo, cause phasic transients is amperometry-measured cholinergic signals via the mechanism of non-steady-state enzyme kinetics in the choline-oxidase-based biosensor.
75. 1. Genetics and Genomics

    GWAS of three molecular traits highlights core genes and pathways alongside a highly polygenic background

    Nasa Sinnott-Armstrong, Sahin Naqvi ... Jonathan K Pritchard

    Across urate, IGF-1, and testosterone, thousands of dispersed variants contribute to heritability, while previously characterized core pathways are highly enriched for associations and reveal feedback mechanisms governing serum biomarker levels.
76. 1. Neuroscience

    Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits

    Mária Ashaber, Yusuke Tomina ... Daniel A Wagenaar

    Voltage-dye imaging followed by serial blockface electron microscopy of the same ganglion reveals structure–function relationships of leech motor behaviors.
77. 1. Developmental Biology

    A β-catenin-driven switch in TCF/LEF transcription factor binding to DNA target sites promotes commitment of mammalian nephron progenitor cells

    Qiuyu Guo, Albert Kim ... Andrew P McMahon

    β-Catenin-mediated expansion of nephron progenitors is independent of direct β-catenin/chromatin engagement, while progenitor induction proceeds with a β-catenin-driven switch of repressive TCFL1/TCFL2 to activating TCF7/LEF1 factors on transcriptionally poised enhancers.
78. 1. Developmental Biology
    2. Physics of Living Systems

    From local resynchronization to global pattern recovery in the zebrafish segmentation clock

    Koichiro Uriu, Bo-Kai Liao ... Luis G Morelli

    Theory explains how transport of gene expression vortices by cell advection may cause intermingled defective and normal segments along the body axis during resynchronization experiments in the zebrafish segmentation clock.
79. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Complex structures of Rsu1 and PINCH1 reveal a regulatory mechanism of the ILK/PINCH/Parvin complex for F-actin dynamics

    Haibin Yang, Leishu Lin ... Cong Yu

    Structural, biochemical, and cell biology study revealed that Rsu1, through binding to PINCH1, inhibits the actin bundling ability of ILK/PINCH/Parvin to regulate the actin dynamics at focal adhesion.
80. 1. Neuroscience

    α-Synuclein plasma membrane localization correlates with cellular phosphatidylinositol polyphosphate levels

    Reeba Susan Jacob, Cédric Eichmann ... Philipp Selenko

    Plasma membrane clusters of the Parkinson's disease protein α-synuclein colocalize with negatively charged phospholipids involved in endocytosis and exocytosis.
81. 1. Developmental Biology
    2. Genetics and Genomics

    Precise base editing for the in vivo study of developmental signaling and human pathologies in zebrafish

    Marion Rosello, Juliette Vougny ... Filippo Del Bene

    Base editors, with improved specificity and expanded PAM sequence recognition, can be used in zebrafish for the rapid and efficient introduction of single base pair mutations.
82. 1. Neuroscience

    Cerebral blood flow and cerebrovascular reactivity are preserved in a mouse model of cerebral microvascular amyloidosis

    Leon P Munting, Marc Derieppe ... Louise van der Weerd

    The causal link between capillary amyloid‑β accumulation in the brain and cerebrovascular dysfunction, previously established in the Tg‑SwDI mouse model, is to be mitigated and remains to be fully uncovered.
83. 1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Basis of specificity for a conserved and promiscuous chromatin remodeling protein

    Drake A Donovan, Johnathan G Crandall ... Jeffrey N McKnight

    The identification of a novel mechanism of chromatin remodeling, including a conserved remodeler domain and regulatory epitopes, provides targets for the design of therapeutics to modulate transcriptional regulation in cells.
84. 1. Immunology and Inflammation
    2. Structural Biology and Molecular Biophysics

    The allosteric modulation of complement C5 by knob domain peptides

    Alex Macpherson, Maisem Laabei ... Jean MH van den Elsen

    Structural and functional analysis of a new class of low-molecular-weight antibody fragments, derived from bovine immunoglobulins, reveals their therapeutic potential against C5, a target for refractory inflammatory diseases.
85. 1. Cell Biology
    2. Developmental Biology

    Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex

    Eric T Hall, Miriam E Dillard ... Stacey K Ogden

    Sonic Hedgehog is transported inside cytonemes with co-receptors that promote contact-mediated signal delivery to initiate rapid, receptor-dependent responses in recipient cells.
86. 1. Neuroscience

    Transsynaptic mapping of Drosophila mushroom body output neurons

    Kristin M Scaplen, Mustafa Talay ... Karla R Kaun

    Transsynaptic mapping of the postsynaptic connections of mushroom body output neurons reveal both divergent and convergent projections allowing for multimodal integration prior to initiation of an output response.
87. 1. Neuroscience

    Dissociation of task engagement and arousal effects in auditory cortex and midbrain

    Daniela Saderi, Zachary P Schwartz ... Stephen V David

    Data collected from two important auditory brain areas distinguish effects of generalized arousal and specific task engagement on neural sensory coding.
88. 1. Computational and Systems Biology
    2. Developmental Biology

    Constructing and optimizing 3D atlases from 2D data with application to the developing mouse brain

    David M Young, Siavash Fazel Darbandi ... Stephan J Sanders

    A computational tool developed to construct three-dimensional anatomical atlases from two-dimensional data generates volumetrically complete and aligned atlases for all stages of development in the Allen Developing Mouse Brain Atlas.
89. 1. Developmental Biology

    EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    Kaustav Mukherjee, Li Xue ... James J Bieker

    Mouse fetal liver macrophages that support erythroid differentiation within erythroblastic islands exhibit a unique and transient expression signature under transcriptional control of EKLF/KLF1.
90. 1. Epidemiology and Global Health
    2. Microbiology and Infectious Disease

    The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types

    Zharko Daniloski, Tristan X Jordan ... Neville E Sanjana

    A pervasive mutation in the Spike protein of the coronavirus that causes COVID-19 results in virions that are up to eightfold more infectious.
91. 1. Immunology and Inflammation
    2. Medicine

    Perinatal granulopoiesis and risk of pediatric asthma

    Benjamin A Turturice, Juliana Theorell ... Patricia W Finn

    Perinatal granulopoiesis and cord blood serum PGLYRP-1, a specific granule protein, are altered prior to onset of childhood asthma and provide potential targets for early identification of at-risk populations.
92. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structure and mechanism of a phage-encoded SAM lyase revises catalytic function of enzyme family

    Xiaohu Guo, Annika Söderholm ... Maria Selmer

    The first structure of a bacteriophage-encoded S-adenosyl methionine degrading enzyme was solved and demonstrated to catalyze a unimolecular lyase reaction occurring at the domain interface of a trimeric structure.
93. 1. Immunology and Inflammation
    2. Neuroscience

    Enhancing mitochondrial activity in neurons protects against neurodegeneration in a mouse model of multiple sclerosis

    Sina C Rosenkranz, Artem A Shaposhnykov ... Manuel A Friese

    Inducing neuronal mitochondrial activity during central nervous system inflammation counteracts inflammation-induced neuronal electron transport chain deficiency and calcium toxicity, thereby protecting against neuronal loss in a multiple sclerosis mouse model.
94. 1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    To lie or not to lie: Super-relaxing with myosins

    Suman Nag, Darshan V Trivedi

    A perspective of the energy-sparing super-relaxed state of myosin and its evolutionary role in modulating skeletal and cardiac muscle power under different physiological and pathophysiological perturbations.
95. 1. Epidemiology and Global Health
    2. Medicine

    Epidemiological transition to mortality and refracture following an initial fracture

    Thao Phuong Ho-Le, Thach S Tran ... Tuan V Nguyen

    The concept of compound fracture risk is redefined to combine the risk that an individual will sustain a fracture and the risk of mortality once the fracture has occurred.
96. 1. Evolutionary Biology
    2. Neuroscience

    Shore crabs reveal novel evolutionary attributes of the mushroom body

    Nicholas Strausfeld, Marcel E Sayre

    Dramatic phenotypic divergence of crustacean mushroom bodies map to phylogenetic lineages, thereby offering unexplored opportunities for relating divergent cognitive centers to different ecologies and behavioral repertoires required to negotiate them.
97. 1. Cell Biology
    2. Genetics and Genomics

    An integrative study of five biological clocks in somatic and mental health

    Rick Jansen, Laura KM Han ... Brenda WJH Penninx

    An integrative study of five biological clocks in somatic and mental health indicate that one's biological age is best reflected by combining aging measures from multiple cellular levels.
98. 1. Epidemiology and Global Health
    2. Genetics and Genomics

    Improved lipidomic profile mediates the effects of adherence to healthy lifestyles on coronary heart disease

    Jiahui Si, Jiachen Li ... Jun Lv

    Lifestyle interventions and statins may target different components of the lipid profile, suggesting that they are not redundant strategies but could be combined for better benefits.
99. 1. Chromosomes and Gene Expression
    2. Neuroscience

    Intronic enhancer region governs transcript-specific Bdnf expression in rodent neurons

    Jürgen Tuvikene, Eli-Eelika Esvald ... Tõnis Timmusk

    An evolutionarily conserved neuron-specific and stimulus-dependent enhancer region downstream of the Bdnf exon III regulates the expression of Bdnf transcripts starting from the upstream 5' exons.
100. 1. Stem Cells and Regenerative Medicine

     Heterogeneity of murine periosteum progenitors involved in fracture healing

     Brya G Matthews, Sanja Novak ... Ivo Kalajzic

     Murine periosteum is highly enriched for osteoprogenitors, many of which express αSMA, but fracture callus chondrocytes are partially derived from other sources.
101. 1. Cell Biology
     2. Developmental Biology

     Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila

     Shichao Yu, Fangzhou Luo, Li Hua Jin

     Rab5 and Rab11 regulate hematopoietic homeostasis in Drosophila, and this process involves the JNK, Toll, and Ras/EGFR signaling pathways and autophagy.
102. 1. Developmental Biology
     2. Evolutionary Biology

     Developmental hourglass and heterochronic shifts in fin and limb development

     Koh Onimaru, Kaori Tatsumi ... Shigehiro Kuraku

     Omics approaches reveal conserved and diversified gene regulation between fin and limb development.
103. 1. Neuroscience

     The distinct roles of calcium in rapid control of neuronal glycolysis and the tricarboxylic acid cycle

     Carlos Manlio Díaz-García, Dylan J Meyer ... Gary Yellen

     When neurons are stimulated, calcium entry into mitochondria upregulates mitochondrial energy production, but glycolytic energy production in the cytosol is stimulated by elevated energy demand, not Ca²⁺ signaling.
104. 1. Neuroscience

     Loss of cortical control over the descending pain modulatory system determines the development of the neuropathic pain state in rats

     Robert AR Drake, Kenneth A Steel ... Anthony E Pickering

     Alterations to brain network communication leading to a progressive loss in descending inhibitory modulation of the spinal cord is a key determinate of pain state development following peripheral nerve injury.
105. 1. Developmental Biology
     2. Neuroscience

     Spinal cord precursors utilize neural crest cell mechanisms to generate hybrid peripheral myelinating glia

     Laura Fontenas, Sarah Kucenas

     Motor exit point (MEP) glia utilize mechanisms most commonly attributed to neural crest cells for their development from spinal cord precursors.
106. 1. Biochemistry and Chemical Biology

     Controlling opioid receptor functional selectivity by targeting distinct subpockets of the orthosteric site

     Rajendra Uprety, Tao Che ... Susruta Majumdar

     The structure-based design established a new approach to control pathway-selective activation of opioid receptors, resulting in new dual MOR/KOR G-protein biased agonist analgesics with attenuated liabilities.
107. 1. Cancer Biology
     2. Immunology and Inflammation

     Human primed ILCPs support endothelial activation through NF-κB signaling

     Giulia Vanoni, Giuseppe Ercolano ... Sara Trabanelli

     Circulating human primed innate lymphoid cell precursors have the potential to functionally induce adhesion molecules' expression in endothelial cells and possibly support the immune cells' infiltration into the tumor site.
108. 1. Microbiology and Infectious Disease
     2. Structural Biology and Molecular Biophysics

     A pentameric protein ring with novel architecture is required for herpesviral packaging

     Allison L Didychuk, Stephanie N Gates ... Britt A Glaunsinger

     The first structures of Kaposi’s sarcoma-associated herpesvirus ORF68 and Epstein–Barr virus BFLF1, conserved and essential proteins required in herpesviruses, reveal new insights into viral genome packaging.
109. 1. Neuroscience

     Single-cell transcriptomes of developing and adult olfactory receptor neurons in Drosophila

     Colleen N McLaughlin, Maria Brbić ... Hongjie Li

     Single-cell transcriptomes of olfactory receptor neurons at multiple developmental stages reveal cell-type-specific gene expression programs that underlie their development and sensory biology.
110. 1. Chromosomes and Gene Expression
     2. Genetics and Genomics

     Dynamic effects of genetic variation on gene expression revealed following hypoxic stress in cardiomyocytes

     Michelle C Ward, Nicholas E Banovich ... Yoav Gilad

     Cellular stress in a disease-relevant cell type uncovers novel genetic effects on gene expression, which is likely relevant for disease.
111. 1. Biochemistry and Chemical Biology
     2. Structural Biology and Molecular Biophysics

     Residue-by-residue analysis of cotranslational membrane protein integration in vivo

     Felix Nicolaus, Ane Metola ... Gunnar von Heijne

     The cotranslational membrane integration of three multispanning Escherichia coli inner membrane proteins is followed using force profile analysis, uncovering unexpected complexities in the membrane integration process.
112. 1. Biochemistry and Chemical Biology
     2. Structural Biology and Molecular Biophysics

     SARS-CoV-2 S protein:ACE2 interaction reveals novel allosteric targets

     Palur V Raghuvamsi, Nikhil K Tulsian ... Ganesh S Anand

     SARS-CoV-2 spike protein binding to receptor angiotensin-converting enzyme 2 allosterically enhances furin proteolysis at distal S1/S2 cleavage sites.
113. 1. Cell Biology
     2. Developmental Biology

     Negative feedback couples Hippo pathway activation with Kibra degradation independent of Yorkie-mediated transcription

     Sherzod A Tokamov, Ting Su ... Richard G Fehon

     Kibra-mediated Hippo complex assembly promotes Kibra degradation in a negative feedback loop that is regulated by mechanical tension.
114. 1. Cell Biology
     2. Developmental Biology

     TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation

     Xiaochen Fan, V Pragathi Masamsetti ... Patrick PL Tam

     Network propagation connects TWIST1 with epigenetic regulators CHD7, CHD8, and WHSC1, which collectively promote the bias toward neural crest while suppressing neural stem cell programmes, and subsequently enhance ectomesenchyme potential.
115. 1. Ecology

     Regularities in species’ niches reveal the world’s climate regions

     Joaquín Calatayud, Magnus Neuman ... Martin Rosvall

     Similarities in climatic niches of terrestrial vertebrates indicate the Earth's climate regions, which differ among groups and from previous plant-based climate classifications.
116. 1. Cancer Biology
     2. Computational and Systems Biology

     shinyDepMap, a tool to identify targetable cancer genes and their functional connections from Cancer Dependency Map data

     Kenichi Shimada, John A Bachman ... Timothy J Mitchison

     shinyDepMap helps users explore the essentiality, selectivity, and function of the genes across hundreds of cancer cell lines and identify cancer drug targets.
117. 1. Epidemiology and Global Health

     Protective effect of Mediterranean-type glucose-6-phosphate dehydrogenase deficiency against Plasmodium vivax malaria

     Ghulam R Awab, Fahima Aaram ... Nicholas J White

     The Mediterranean variant of glucose-6-phosphate dehydrogenase deficiency confers a strong gene-dose proportional protective effect against symptomatic Plasmodium vivax malaria in the Pashtun ethnic group.
118. 1. Computational and Systems Biology

     Cycles, sources, and sinks: Conceptualizing how phosphate balance modulates carbon flux using yeast metabolic networks

     Ritu Gupta, Sunil Laxman

     A theoretical conceptualization of how phosphates control metabolic information flow and predictably regulate the metabolic state of the cell.
119. 1. Developmental Biology
     2. Genetics and Genomics

     Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in development

     Ryan J Geusz, Allen Wang ... Maike Sander

     Analysis of epigenome maps from human pancreatic progenitors and functional validation in zebrafish identify LAMA1 and CRB2 as type 2 diabetes risk-associated genes with roles in pancreatic development.
120. 1. Neuroscience
     2. Stem Cells and Regenerative Medicine

     Dyshomeostatic modulation of Ca²⁺-activated K⁺ channels in a human neuronal model of KCNQ2 encephalopathy

     Dina Simkin, Kelly A Marshall ... Evangelos Kiskinis

     An inducedpluripotent stem cell (iPSC)-based model of KCNQ2-associated developmental epileptic encephalopathy suggests that disease is driven by dyshomeostaic neuronal mechanisms that are downstream of loss of M-current.
121. 1. Biochemistry and Chemical Biology

     Membrane-partitioned cell wall synthesis in mycobacteria

     Alam García-Heredia, Takehiro Kado ... M Sloan Siegrist

     Mycobacteria employ plasma membrane compartments to organize their cell wall synthesis, and the finalized cell wall compartmentalizes the plasma membrane to promote an environment conducive to its own synthesis.
122. 1. Epidemiology and Global Health
     2. Medicine

     Quantifying the impact of quarantine duration on COVID-19 transmission

     Peter Ashcroft, Sonja Lehtinen ... Sebastian Bonhoeffer

     Test-and-release quarantine strategies for traced contacts of confirmed SARS-CoV-2 cases and returning travellers can reduce average quarantine durations while remaining as effective as 10 days of quarantine without testing.
123. 1. Evolutionary Biology
     2. Genetics and Genomics

     Evolutionary dynamics of transposable elements in bdelloid rotifers

     Reuben W Nowell, Christopher G Wilson ... Timothy G Barraclough

     An investigation of transposable element evolution in a group of long-term asexual animals shows that many of the predicted effects of asexuality are not observed.
124. 1. Physics of Living Systems

     Single molecule microscopy reveals key physical features of repair foci in living cells

     Judith Miné-Hattab, Mathias Heltberg ... Angela Taddei

     Upon DNA damage, Rad52 forms a membraneless sub-compartment where Rad52 molecules are highly dynamic and share properties with liquid-liquid phase-separated molecules, reflecting the existence of a liquid droplet around damaged DNA.
125. 1. Immunology and Inflammation

     Cell-density independent increased lymphocyte production and loss rates post-autologous HSCT

     Mariona Baliu-Piqué, Vera van Hoeven ... Kiki Tesselaar

     Altered lymphocyte dynamics after hematopoietic stem cell transplantation cannot solely be explained by low lymphocyte numbers.
126. 1. Neuroscience

     Coupling between fast and slow oscillator circuits in Cancer borealis is temperature-compensated

     Daniel Powell, Sara A Haddad ... Eve Marder

     The mechanism that couples two rhythmic circuits is maintained over a large temperature range although both circuits are temperature-sensitive.
127. 1. Biochemistry and Chemical Biology
     2. Developmental Biology

     Hormone-sensitive lipase couples intergenerational sterol metabolism to reproductive success

     Christoph Heier, Oskar Knittelfelder ... Ronald P Kühnlein

     An ancestral enzyme tailors allocation of essential lipid resources to the progeny and connects maternal and embryonic sterol metabolism for reproductive success.
128. 1. Neuroscience

     Spatial modulation of visual responses arises in cortex with active navigation

     E Mika Diamanti, Charu Bai Reddy ... Matteo Carandini

     Spatial modulation along the visual pathway arises in the cortex and strengthens with active navigation and experience.
129. 1. Chromosomes and Gene Expression

     Dimerisation of the PICTS complex via LC8/Cut-up drives co-transcriptional transposon silencing in Drosophila

     Evelyn L Eastwood, Kayla A Jara ... Gregory J Hannon

     Heterochromatin formation at transposon loci depends on dimerisation of the effector complex that elicits co-transcriptional silencing and this requirement is fulfilled by co-option of the conserved dimerisation hub protein, Cut-up/LC8.
130. 1. Neuroscience

     Distinct neural networks for the volitional control of vocal and manual actions in the monkey homologue of Broca's area

     Natalja Gavrilov, Andreas Nieder

     Single-neuron recordings in the inferior frontal cortex of trained macaques show neurons that are selective to the volitional preparation of either vocal or manual actions.
131. 1. Cell Biology

     Tripartite suppression of fission yeast TORC1 signaling by the GATOR1-Sea3 complex, the TSC complex, and Gcn2 kinase

     Tomoyuki Fukuda, Fajar Sofyantoro ... Kazuhiro Shiozaki

     Sea3, a presumed subunit of the GATOR2 complex in fission yeast, is required for the GATOR1 function that attenuates TORC1 in parallel with the TSC complex and Gcn2 kinase.
132. 1. Immunology and Inflammation

     Gut Helicobacter presentation by multiple dendritic cell subsets enables context-specific regulatory T cell generation

     Emilie V Russler-Germain, Jaeu Yi ... Chyi-Song Hsieh

     In vitro and in vivo studies show that multiple dendritic cell subsets are capable of presenting gut Helicobacter antigens to induce regulatory T cells.
133. 1. Neuroscience

     Drosophila uses a tripod gait across all walking speeds, and the geometry of the tripod is important for speed control

     Chanwoo Chun, Tirthabir Biswas, Vikas Bhandawat

     Drosophila uses a gait close to the tripod throughout its speed range, and changes in speed are accompanied by changes in the geometry of the tripod.
134. 1. Immunology and Inflammation

     Gross ways to live long: Parasitic worms as an anti-inflammaging therapy?

     Bruce Zhang, David Gems

     The absence of helminth parasites in developed countries may be exacerbating pathological, age-associated inflammation, known as inflammaging, suggesting that helminth therapies could provide protection against age-related disease.
135. 1. Neuroscience

     Seizures are a druggable mechanistic link between TBI and subsequent tauopathy

     Hadeel Alyenbaawi, Richard Kanyo ... W Ted Allison

     A traumatic brain injury model is invented for larval zebrafish and applied to a new fluorescent 'tauopathy reporter fish', revealing a role for seizures in progression towards dementias.
136. 1. Cancer Biology

     SATB2 induction of a neural crest mesenchyme-like program drives melanoma invasion and drug resistance

     Maurizio Fazio, Ellen van Rooijen ... Leonard I Zon

     A genetic discovery screen for epigenetic factors accelerating melanoma development in vivo identifies SATB2 as a driver of tumor invasion and resistance to FDA-approved BRAF-targeted inhibitor Vemurafenib.
137. 1. Developmental Biology
     2. Stem Cells and Regenerative Medicine

     Regenerative neurogenic response from glia requires insulin-driven neuron-glia communication

     Neale J Harrison, Elizabeth Connolly ... Alicia Hidalgo

     Neuronal Ia-2 and glial Kon coordinate an injury-response insulin relay that restores glial cell populations and induces neural stem cells from glia, enabling central nervous system regeneration.
138. 1. Epidemiology and Global Health

     A modular approach to integrating multiple data sources into real-time clinical prediction for pediatric diarrhea

     Ben J Brintz, Benjamin Haaland ... Daniel T Leung

     Using a modular approach to integrate multiple data sources for the prediction of diarrhea etiology has the potential to reduce inappropriate antibiotics prescription in low-resource settings.
139. 1. Neuroscience

     Leptin receptor neurons in the dorsomedial hypothalamus regulate diurnal patterns of feeding, locomotion, and metabolism

     Chelsea L Faber, Jennifer D Deem ... Gregory J Morton

     Synchronized feeding and metabolic patterns with environmental light–dark cycles is critical to maintain energy homeostasis and requires the activity of leptin-receptor neurons in the dorsomedial hypothalamus.
140. 1. Cell Biology
     2. Developmental Biology

     Celsr1 adhesive interactions mediate the asymmetric organization of planar polarity complexes

     Sara N Stahley, Lena P Basta ... Danelle Devenport

     Planar cell polarity protein Celsr1 clusters into punctate assemblies through lateral cis-interactions to function as an organizer of intercellular Frizzled6 and Vangl2 asymmetry.
141. 1. Computational and Systems Biology

     Emergence and propagation of epistasis in metabolic networks

     Sergey Kryazhimskiy

     Mutations that affect a metabolic network generically exhibit epistasis, which propagates to higher level phenotypes, such as fitness, carrying some information about the network’s topology.
142. 1. Cancer Biology

     Convergent organization of aberrant MYB complex controls oncogenic gene expression in acute myeloid leukemia

     Sumiko Takao, Lauren Forbes ... Alex Kentsis

     Definition of leukemia gene expression mechanisms reveals general principles of cancer gene control and offers a pharmacologic strategy for its therapeutic reprogramming.
143. 1. Cell Biology

     A small-molecule ICMT inhibitor delays senescence of Hutchinson-Gilford progeria syndrome cells

     Xue Chen, Haidong Yao ... Martin O Bergo

     Knockout of the methyltransferase ICMT prevents progerin methylation and improves survival in mice with Hutchinson-Gilford progeria syndrome (HGPS) and an ICMT inhibitor delays senescence and stimulates proliferation of HGPS cells.
144. 1. Biochemistry and Chemical Biology
     2. Medicine

     Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice

     Jumpei Ito, Shigemiki Omiya ... Kinya Otsu

     Iron derived from autophagy-mediated ferritin degradation in response to pressure overload induces lipid peroxidation, necrotic cardiomyocyte death, and heart failure in mice.
145. 1. Cell Biology

     Robustness of the microtubule network self-organization in epithelia

     Aleksandra Z Płochocka, Miguel Ramirez Moreno ... Lyubov Chumakova

     Computational, theoretical, and in vivo studies reveal that in epithelia the self-organization of apical microtubules is robustly determined by cell geometry and minus-end distribution, not organism environment or genetics.
146. 1. Cell Biology
     2. Structural Biology and Molecular Biophysics

     Pre-existing bilayer stresses modulate triglyceride accumulation in the ER versus lipid droplets

     Valeria Zoni, Rasha Khaddaj ... Stefano Vanni

     In parallel to protein-driven processes, characteristic physicochemical properties of the endoplasmic reticulum membrane modulate intracellular fat accumulation and lipid droplet formation.
147. 1. Neuroscience

     Laminar-specific cortico-cortical loops in mouse visual cortex

     Hedi Young, Beatriz Belbut ... Leopoldo Petreanu

     Ascending and descending cortico-cortical inputs are stronger on projection neurons that project back to the source of the inputs, forming selective interareal loops in deep but not superficial cortical layers.
148. 1. Chromosomes and Gene Expression

     Surprising phenotypic diversity of cancer-associated mutations of Gly 34 in the histone H3 tail

     Brandon R Lowe, Rajesh K Yadav ... Janet F Partridge

     H3-G34R, V, and W oncohistonesin fission yeast cause differential K36 modification, DNA damage sensitivity and genome stability outcomes, highlighting the need for a thorough evaluation of distinct mutations.
149. 1. Cell Biology
     2. Physics of Living Systems

     Non-genetic inheritance restraint of cell-to-cell variation

     Harsh Vashistha, Maryam Kohram, Hanna Salman

     Quantitative measurement of epigenetic memory and inheritance dynamics.
150. 1. Epidemiology and Global Health
     2. Microbiology and Infectious Disease

     In-host population dynamics of Mycobacterium tuberculosis complex during active disease

     Roger Vargas, Luca Freschi ... Maha Reda Farhat

     Bulk whole genome sequencing data can be used to study the genetic variation present in pathogenic bacterial populations over the time-course of a single infection within a host.
151. 1. Chromosomes and Gene Expression

     The human origin recognition complex is essential for pre-RC assembly, mitosis, and maintenance of nuclear structure

     Hsiang-Chen Chou, Kuhulika Bhalla ... Bruce Stillman

     The initiation of human genome replication requires the six-subunit origin recognition complex (ORC) and CDC6, with ORC playing additional roles during mitosis and in organization of the cell nucleus.
152. 1. Chromosomes and Gene Expression
     2. Computational and Systems Biology

     Global, cell non-autonomous gene regulation drives individual lifespan among isogenic C. elegans

     Holly E Kinser, Matthew C Mosley ... Zachary Pincus

     Expression of almost half of a library of fluorescent reporters distinguish long- from short-lived individual Caenorhabditis elegans, suggesting that organism-wide differences in gene expression drive future lifespan.
153. 1. Neuroscience

     Performance in even a simple perceptual task depends on mouse secondary visual areas

     Hannah C Goldbach, Bradley Akitake ... Mark H Histed

     In a simple visual task, that in principle could be performed using information in V1 alone, perturbations of secondary visual areas impair perception.
154. 1. Evolutionary Biology
     2. Genetics and Genomics

     Evolutionary transcriptomics implicates HAND2 in the origins of implantation and regulation of gestation length

     Mirna Marinić, Katelyn Mika ... Vincent J Lynch

     The transcription factor HAND2 evolved uterine expression during the origins of pregnancy and may be important for maternal-fetal immune signaling and parturition.
155. 1. Developmental Biology
     2. Neuroscience

     Dual midbrain and forebrain origins of thalamic inhibitory interneurons

     Polona Jager, Gerald Moore ... Alessio Delogu

     Thalamic interneurons in the mouse thalamus are often overlooked because of their extremely low numbers, however they are developmentally complex and related to those of larger-brained species.
156. 1. Computational and Systems Biology
     2. Genetics and Genomics

     The genetic landscape for amyloid beta fibril nucleation accurately discriminates familial Alzheimer’s disease mutations

     Mireia Seuma, Andre J Faure ... Benedetta Bolognesi

     A massively parallel analysis of the effects of mutations on amyloid beta nucleation provide the first comprehensive atlas of how mutations alter the formation of amyloid fibrils.