Integrons deploy a variety of adaptive strategies including excision, shuffling, and duplication of cassettes that foster rapid bacterial adaptation and resistance evolution while protecting the genomic integrity of the host.
Dual resin casting of multiple lumenized systems followed by microCT allows visualization, digitalization, and quantification of architectural parameters defining the vascular and biliary systems in liver revealing new phenotypes.
Serial-Block-Face Scanning Electron Microscopy (SBF-SEM) associated with biomolecular analysis show that chloroplast differentiation proceeds by distinct ‘structure establishment’ and ‘chloroplast proliferation’ phases, each with differential protein and lipid regulation.
SWELL1 is required for basal, stretch, and flow-mediated endothelial AKT-eNOS signaling in vitro and protects against angiotensin-induced hypertension and diabetes-associated vascular dysfunction in vivo.
Unbiased and automatic annotation using structured prediction framework with efficiently built data-driven atlases is more accurate than registration-based methods for cell identifications in dense images and enables fast whole-brain analysis.
Systematic analysis of C. elegans zygotes manipulated to divide equally demonstrates that daughter cell size asymmetry is critical for proper cell cycle timing, positioning, and fates during subsequent embryogenesis.
3D reconstruction for an insect provides new internal structures at nanometer resolution, and the reconstructed feeding insect reveals unexpected contraction of stylet protractors and suggested a novel phloem sap-sucking model.
Neuronal interacting proteome reveals that the cellular dynamics of the lissencephaly-associated extracellular matrix receptor dystroglycan are governed by the exocyst complex, which is key for proper brain assembly.
FlyBrainLab is an open-source computing platform that integrates 3D exploration and visualization of diverse Drosophila connectomic/synaptomic datasets with interactive exploration of the functional logic of modeled executable brain circuits.
The lamin A/C binding protein LAP2α inhibits formation of higher order lamin structures in the nuclear interior in a lamin A/C-phosphorylation-independent manner, thereby regulating chromatin mobility.
Virus infection of the central nervous system disrupts the homeostasis of the immune-neural-synaptic axis via induction of pleiotropic genes with an unintended off-target negative impact on the neurotransmission.
RNA profiles from lungs of mice exposed to intermittent hypoxia shared similarity with gene expression changes in human lung from patients with pulmonary diseases, including pulmonary hypertension, COPD, and asthma.
Analysis of slow wave brain state unravels the functional connectivity and the biological substrate of the rodent dorsolateral and dorsomedial striatum, demonstrating its organization in two non-overlapping circuits.
Comprehensive developmental in situ analysis of the Drosophila lymph gland provides markers highlighting blood progenitor diversity and reveals that JAK-STAT signaling prevents posterior progenitor differentiation, promoting survival after immune challenge.
A comparative analysis of human and chimpanzee polyadenylation site usage establishes alternative polyadenylation as another key mechanism underlying the genetic regulation of transcript and protein expression levels in primates.
The response from discrete stages of the early auditory pathway can be measured by subtle manipulations to long-form natural speech stimuli paired with deconvolution analysis of electroencephalography data.
Single-cell dissection of recent neural crest derivatives in the vertebrate zebrafish reveals diverse transcriptomic signatures among differentiating posterior cell types during the embryonic to larval stage transition.
BLITZ system enables proximity-dependent biotin labelling in live zebrafish embryos with cell and tissue specificity, providing a versatile and valuable tool for proteomic discovery using the zebrafish model.
COVID-19 will have an ongoing impact on antimicrobial resistance acquisition, transmission, and burden, requiring the close attention of researchers globally to generate a complete evidence base for the shifted dynamics.
An iron-sensitive gene cluster encodes proteins that co-localize with phytotransferrin endosomes and are involved in key intracellular iron transformation and trafficking processes in a model marine diatom.
Studying individual Achilles tendon geometry and interface sliding capacity may allow prediction of injury sites, and targeted training on specific muscle-(sub-)tendon units may boost beneficial outcomes for Achilles tendinopathy.
Theory explains how transport of gene expression vortices by cell advection may cause intermingled defective and normal segments along the body axis during resynchronization experiments in the zebrafish segmentation clock.
Across urate, IGF-1, and testosterone, thousands of dispersed variants contribute to heritability, while previously characterized core pathways are highly enriched for associations and reveal feedback mechanisms governing serum biomarker levels.
β-Catenin-mediated expansion of nephron progenitors is independent of direct β-catenin/chromatin engagement, while progenitor induction proceeds with a β-catenin-driven switch of repressive TCFL1/TCFL2 to activating TCF7/LEF1 factors on transcriptionally poised enhancers.
Fast fluctuations of oxygen, linked to behavioral and neural dynamics in vivo, cause phasic transients is amperometry-measured cholinergic signals via the mechanism of non-steady-state enzyme kinetics in the choline-oxidase-based biosensor.
Functional brain scans of human participants show that the brain encodes other people's attention in enough richness to distinguish whether that attention was directed exogenously (stimulus-driven) or endogenously (internally driven).
The identification of a novel mechanism of chromatin remodeling, including a conserved remodeler domain and regulatory epitopes, provides targets for the design of therapeutics to modulate transcriptional regulation in cells.
The causal link between capillary amyloid‑β accumulation in the brain and cerebrovascular dysfunction, previously established in the Tg‑SwDI mouse model, is to be mitigated and remains to be fully uncovered.
Structural and functional analysis of a new class of low-molecular-weight antibody fragments, derived from bovine immunoglobulins, reveals their therapeutic potential against C5, a target for refractory inflammatory diseases.
Transsynaptic mapping of the postsynaptic connections of mushroom body output neurons reveal both divergent and convergent projections allowing for multimodal integration prior to initiation of an output response.
A computational tool developed to construct three-dimensional anatomical atlases from two-dimensional data generates volumetrically complete and aligned atlases for all stages of development in the Allen Developing Mouse Brain Atlas.
Inducing neuronal mitochondrial activity during central nervous system inflammation counteracts inflammation-induced neuronal electron transport chain deficiency and calcium toxicity, thereby protecting against neuronal loss in a multiple sclerosis mouse model.
A perspective of the energy-sparing super-relaxed state of myosin and its evolutionary role in modulating skeletal and cardiac muscle power under different physiological and pathophysiological perturbations.
Perinatal granulopoiesis and cord blood serum PGLYRP-1, a specific granule protein, are altered prior to onset of childhood asthma and provide potential targets for early identification of at-risk populations.
The first structure of a bacteriophage-encoded S-adenosyl methionine degrading enzyme was solved and demonstrated to catalyze a unimolecular lyase reaction occurring at the domain interface of a trimeric structure.
Dramatic phenotypic divergence of crustacean mushroom bodies map to phylogenetic lineages, thereby offering unexplored opportunities for relating divergent cognitive centers to different ecologies and behavioral repertoires required to negotiate them.
Alterations to brain network communication leading to a progressive loss in descending inhibitory modulation of the spinal cord is a key determinate of pain state development following peripheral nerve injury.
The structure-based design established a new approach to control pathway-selective activation of opioid receptors, resulting in new dual MOR/KOR G-protein biased agonist analgesics with attenuated liabilities.
Circulating human primed innate lymphoid cell precursors have the potential to functionally induce adhesion molecules' expression in endothelial cells and possibly support the immune cells' infiltration into the tumor site.
The cotranslational membrane integration of three multispanning Escherichia coli inner membrane proteins is followed using force profile analysis, uncovering unexpected complexities in the membrane integration process.
When neurons are stimulated, calcium entry into mitochondria upregulates mitochondrial energy production, but glycolytic energy production in the cytosol is stimulated by elevated energy demand, not Ca2+ signaling.
Network propagation connects TWIST1 with epigenetic regulators CHD7, CHD8, and WHSC1, which collectively promote the bias toward neural crest while suppressing neural stem cell programmes, and subsequently enhance ectomesenchyme potential.
The first structures of Kaposi’s sarcoma-associated herpesvirus ORF68 and Epstein–Barr virus BFLF1, conserved and essential proteins required in herpesviruses, reveal new insights into viral genome packaging.
An inducedpluripotent stem cell (iPSC)-based model of KCNQ2-associated developmental epileptic encephalopathy suggests that disease is driven by dyshomeostaic neuronal mechanisms that are downstream of loss of M-current.
Test-and-release quarantine strategies for traced contacts of confirmed SARS-CoV-2 cases and returning travellers can reduce average quarantine durations while remaining as effective as 10 days of quarantine without testing.
Analysis of epigenome maps from human pancreatic progenitors and functional validation in zebrafish identify LAMA1 and CRB2 as type 2 diabetes risk-associated genes with roles in pancreatic development.
Mycobacteria employ plasma membrane compartments to organize their cell wall synthesis, and the finalized cell wall compartmentalizes the plasma membrane to promote an environment conducive to its own synthesis.
The Mediterranean variant of glucose-6-phosphate dehydrogenase deficiency confers a strong gene-dose proportional protective effect against symptomatic Plasmodium vivax malaria in the Pashtun ethnic group.
Upon DNA damage, Rad52 forms a membraneless sub-compartment where Rad52 molecules are highly dynamic and share properties with liquid-liquid phase-separated molecules, reflecting the existence of a liquid droplet around damaged DNA.
Heterochromatin formation at transposon loci depends on dimerisation of the effector complex that elicits co-transcriptional silencing and this requirement is fulfilled by co-option of the conserved dimerisation hub protein, Cut-up/LC8.
Synchronized feeding and metabolic patterns with environmental light–dark cycles is critical to maintain energy homeostasis and requires the activity of leptin-receptor neurons in the dorsomedial hypothalamus.
The absence of helminth parasites in developed countries may be exacerbating pathological, age-associated inflammation, known as inflammaging, suggesting that helminth therapies could provide protection against age-related disease.
Knockout of the methyltransferase ICMT prevents progerin methylation and improves survival in mice with Hutchinson-Gilford progeria syndrome (HGPS) and an ICMT inhibitor delays senescence and stimulates proliferation of HGPS cells.
A genetic discovery screen for epigenetic factors accelerating melanoma development in vivo identifies SATB2 as a driver of tumor invasion and resistance to FDA-approved BRAF-targeted inhibitor Vemurafenib.
H3-G34R, V, and W oncohistonesin fission yeast cause differential K36 modification, DNA damage sensitivity and genome stability outcomes, highlighting the need for a thorough evaluation of distinct mutations.
Ascending and descending cortico-cortical inputs are stronger on projection neurons that project back to the source of the inputs, forming selective interareal loops in deep but not superficial cortical layers.
Expression of almost half of a library of fluorescent reporters distinguish long- from short-lived individual Caenorhabditis elegans, suggesting that organism-wide differences in gene expression drive future lifespan.
Computational, theoretical, and in vivo studies reveal that in epithelia the self-organization of apical microtubules is robustly determined by cell geometry and minus-end distribution, not organism environment or genetics.